(Circular) (Six-base) MAPSORT of: pFN21AB9845.seq Check: 4922 from: 1 to: 5824 pFN21AB9845 5824 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3634 278 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2175 3167 1937 Size: 238 992 4594 Fragments arranged by size: 4594 992 238 Acc65I G'GTAC_C Cuts at: 2576 3145 2576 Size: 569 5255 AclI AA'CG_TT Cuts at: 1563 3951 4324 1563 Size: 2388 373 3063 Fragments arranged by size: 3063 2388 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3887 5271 5539 1632 Size: 2255 1384 268 1917 Fragments arranged by size: 2255 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5627 AflIII A'CryG_T Cuts at: 1203 2271 4723 5700 1203 Size: 1068 2452 977 1327 Fragments arranged by size: 2452 1327 1068 977 AgeI A'CCGG_T Cuts at: 2448 4655 2448 Size: 2207 3617 AhdI GACnn_n'nnGTC Cuts at: 2934 4553 2934 Size: 1619 4205 AleI CACnn'nnGTG Cuts at: 1166 2669 1166 Size: 1503 4321 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5824 AlwNI CAG_nnn'CTG Cuts at: 3567 5139 3567 Size: 1572 4252 ApaI G_GGCC'C Cuts at: 2863 2863 Size: 5824 ApaLI G'TGCA_C Cuts at: 2662 3881 5037 2662 Size: 1219 1156 3449 Fragments arranged by size: 3449 1219 1156 ApoI r'AATT_y Cuts at: 1493 3049 3133 3359 3511 5620 1493 Size: 1556 84 226 152 2109 1697 Fragments arranged by size: 2109 1697 1556 226 152 84 AseI AT'TA_AT Cuts at: 160 1032 2720 4378 160 Size: 872 1688 1658 1606 Fragments arranged by size: 1688 1658 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 AvaI C'yCGr_G Cuts at: 1870 1943 3012 3149 1870 Size: 73 1069 137 4545 Fragments arranged by size: 4545 1069 137 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2443 2476 2560 2568 2593 2601 2443 Size: 33 84 8 25 8 5666 Fragments arranged by size: 5666 84 33 25 8 8 BamHI G'GATC_C Cuts at: 3154 3521 3154 Size: 367 5457 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2576 3145 4600 618 Size: 325 217 627 789 569 1455 1842 Fragments arranged by size: 1842 1455 789 627 569 325 217 BanII G_rGCy'C Cuts at: 1391 2863 3143 1391 Size: 1472 280 4072 Fragments arranged by size: 4072 1472 280 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2227 3075 928 Size: 971 328 848 3677 Fragments arranged by size: 3677 971 848 328 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4015 4049 1903 Size: 34 2078 34 3678 Fragments arranged by size: 3678 2078 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3716 4926 3716 Size: 1210 4614 BclI T'GATC_A Cuts at: 1548 1548 Size: 5824 BfrBI ATG'CAT Cuts at: 2436 2436 Size: 5824 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2057 4435 136 Size: 107 122 71 631 605 385 2378 1525 Fragments arranged by size: 2378 1525 631 605 385 122 107 71 BglII A'GATC_T Cuts at: 2746 5819 2746 Size: 3073 2751 BlpI GC'TnA_GC Cuts at: 3241 3241 Size: 5824 Bme1580I G_kGCm'C Cuts at: 1790 2446 2666 2863 3885 5041 1790 Size: 656 220 197 1022 1156 2573 Fragments arranged by size: 2573 1156 1022 656 220 197 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5824 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3555 4513 442 Size: 416 53 83 399 24 2138 958 1753 Fragments arranged by size: 2138 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5824 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2272 4484 1239 Size: 1033 2212 2579 Fragments arranged by size: 2579 2212 1033 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2459 2875 3949 4814 5112 2459 Size: 416 1074 865 298 3171 Fragments arranged by size: 3171 1074 865 416 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4487 882 Size: 617 2988 2219 Fragments arranged by size: 2988 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2305 2919 3615 493 Size: 711 1101 614 696 2702 Fragments arranged by size: 2702 1101 711 696 614 BsaBI GATnn'nnATC Cuts at: 2513 5818 2513 Size: 3305 2519 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3631 4013 4648 275 Size: 53 83 186 936 2098 382 635 1451 Fragments arranged by size: 2098 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2448 4257 4655 4929 5076 5487 2448 Size: 1809 398 274 147 411 2785 Fragments arranged by size: 2785 1809 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5599 Fragments arranged by size: 5599 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2104 2751 2782 1186 Size: 918 647 31 4228 Fragments arranged by size: 4228 918 647 31 BseYI C'CCAG_C Cuts at: 2881 5027 5523 2881 Size: 2146 496 3182 Fragments arranged by size: 3182 2146 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5251 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2094 2670 3274 4035 5063 1540 Size: 275 186 93 576 604 761 1028 2301 Fragments arranged by size: 2301 1028 761 604 576 275 186 93 BsiHKAI G_wGCw'C Cuts at: 2666 3885 3970 5041 2666 Size: 1219 85 1071 3449 Fragments arranged by size: 3449 1219 1071 85 BsmI GAATG_Cn' Cuts at: 2509 3335 3428 2509 Size: 826 93 4905 Fragments arranged by size: 4905 826 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2116 2116 Size: 5824 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2446 2666 2863 3143 3885 3970 5041 Size: 399 656 220 197 280 742 85 1071 Cuts at: 5041 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 656 399 280 220 197 85 BspEI T'CCGG_A Cuts at: 5487 5487 Size: 5824 BspHI T'CATG_A Cuts at: 3713 5443 3713 Size: 1730 4094 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2172 2643 3179 844 Size: 1328 471 536 3489 Fragments arranged by size: 3489 1328 536 471 BsrBI CCG'CTC Cuts at: 1394 1823 2256 3711 1394 Size: 429 433 1455 3507 Fragments arranged by size: 3507 1455 433 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2892 4319 4493 65 Size: 1183 1644 1427 174 1396 Fragments arranged by size: 1644 1427 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2023 2060 2167 2448 2487 4468 4655 Size: 110 213 37 107 281 39 1981 187 Cuts at: 4655 1700 Size: 2869 Fragments arranged by size: 2869 1981 281 213 187 110 107 39 37 BsrGI T'GTAC_A Cuts at: 96 2268 96 Size: 2172 3652 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5824 BssSI C'ACGA_G Cuts at: 3884 4896 3884 Size: 1012 4812 BstAPI GCAn_nnn'nTGC Cuts at: 794 2891 794 Size: 2097 3727 BstBI TT'CG_AA Cuts at: 4628 4628 Size: 5824 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5824 BstYI r'GATC_y Cuts at: 1972 2355 2746 3154 3521 3906 3923 5375 5461 Size: 383 391 408 367 385 17 1452 86 Cuts at: 5461 5473 5819 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 408 391 385 383 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2670 513 Size: 552 1605 3667 Fragments arranged by size: 3667 1605 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5085 Fragments arranged by size: 5085 683 56 BtsI GCAGTG_nn' Cuts at: 2100 3340 4134 4154 2100 Size: 1240 794 20 3770 Fragments arranged by size: 3770 1240 794 20 ClaI AT'CG_AT Cuts at: 1554 2777 1554 Size: 1223 4601 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5789 DraI TTT'AAA Cuts at: 3128 3128 Size: 5824 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4831 809 Size: 507 3515 1802 Fragments arranged by size: 3515 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2021 2058 2217 Size: 54 1425 50 275 18 191 37 159 Cuts at: 2217 2289 2673 3271 4160 5789 8 Size: 72 384 598 889 1629 43 Fragments arranged by size: 1629 1425 889 598 384 275 191 159 72 54 50 43 37 18 EagI C'GGCC_G Cuts at: 1537 1812 3271 1537 Size: 275 1459 4090 Fragments arranged by size: 4090 1459 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2570 3754 1353 Size: 1217 1184 3423 Fragments arranged by size: 3423 1217 1184 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4411 4785 4931 387 Size: 1064 138 2822 374 146 1280 Fragments arranged by size: 2822 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2272 3887 4484 5271 5539 1239 Size: 393 640 1615 597 787 268 1524 Fragments arranged by size: 1615 1524 787 640 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2859 2859 Size: 5824 EcoRI G'AATT_C Cuts at: 3133 3511 3133 Size: 378 5446 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2164 3045 2164 Size: 881 4943 FspI TGC'GCA Cuts at: 4330 4330 Size: 5824 HaeII r_GCGC'y Cuts at: 1678 4971 1678 Size: 3293 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2606 2638 2920 2952 4465 Size: 32 135 32 1034 32 282 32 1513 Cuts at: 4465 4497 4539 4571 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1513 1034 282 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3168 3414 669 Size: 988 281 1230 246 3079 Fragments arranged by size: 3079 1230 988 281 246 HindIII A'AGCT_T Cuts at: 748 3027 748 Size: 2279 3545 HpaI GTT'AAC Cuts at: 3414 3414 Size: 5824 KpnI G_GTAC'C Cuts at: 2580 3149 2580 Size: 569 5255 MfeI C'AATT_G Cuts at: 3423 3423 Size: 5824 MluI A'CGCG_T Cuts at: 5700 5700 Size: 5824 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4938 5122 1305 Size: 3633 184 2007 Fragments arranged by size: 3633 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2219 2675 10 Size: 54 1425 343 387 456 3159 Fragments arranged by size: 3159 1425 456 387 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2225 2264 2540 2669 3782 4141 4300 Size: 648 1059 39 276 129 1113 359 159 Cuts at: 4300 518 Size: 2042 Fragments arranged by size: 2042 1113 1059 648 359 276 159 129 39 MspA1I CmG'CkG Cuts at: 1606 2457 3240 3917 5065 5310 1606 Size: 851 783 677 1148 245 2120 Fragments arranged by size: 2120 1148 851 783 677 245 NaeI GCC'GGC Cuts at: 2025 2169 2489 2025 Size: 144 320 5360 Fragments arranged by size: 5360 320 144 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5272 NdeI CA'TA_TG Cuts at: 387 387 Size: 5824 NgoMIV G'CCGG_C Cuts at: 2023 2167 2487 2023 Size: 144 320 5360 Fragments arranged by size: 5360 320 144 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5824 NotI GC'GGCC_GC Cuts at: 3271 3271 Size: 5824 NsiI A_TGCA'T Cuts at: 2438 2438 Size: 5824 NspI r_CATG'y Cuts at: 1132 1229 2275 2436 2662 3182 4727 1132 Size: 97 1046 161 226 520 1545 2229 Fragments arranged by size: 2229 1545 1046 520 226 161 97 PasI CC'CwG_GG Cuts at: 1348 2482 1348 Size: 1134 4690 PciI A'CATG_T Cuts at: 2271 4723 2271 Size: 2452 3372 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5824 PmeI GTTT'AAAC Cuts at: 3128 3128 Size: 5824 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3893 3925 5406 5438 3893 Size: 32 1481 32 4279 Fragments arranged by size: 4279 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5824 PsiI TTA'TAA Cuts at: 3394 3394 Size: 5824 PspOMI G'GGCC_C Cuts at: 2859 2859 Size: 5824 PstI C_TGCA'G Cuts at: 830 2385 3176 830 Size: 1555 791 3478 Fragments arranged by size: 3478 1555 791 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5824 RsrII CG'GwC_CG Cuts at: 2063 2063 Size: 5824 SalI G'TCGA_C Cuts at: 1936 3166 1936 Size: 1230 4594 SbfI CC_TGCA'GG Cuts at: 3176 3176 Size: 5824 ScaI AGT'ACT Cuts at: 4072 5784 4072 Size: 1712 4112 SexAI A'CCwGG_T Cuts at: 2784 2784 Size: 5824 SfcI C'TryA_G Cuts at: 826 1045 2381 2653 3172 4307 4988 5179 826 Size: 219 1336 272 519 1135 681 191 1471 Fragments arranged by size: 1471 1336 1135 681 519 272 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5824 SmaI CCC'GGG Cuts at: 1872 3151 1872 Size: 1279 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2474 2854 3928 4829 5091 820 Size: 197 926 531 380 1074 901 262 1553 Fragments arranged by size: 1553 1074 926 901 531 380 262 197 SnaBI TAC'GTA Cuts at: 493 3615 493 Size: 3122 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5824 SphI G_CATG'C Cuts at: 1132 3182 1132 Size: 2050 3774 SspI AAT'ATT Cuts at: 5 52 3085 3748 5 Size: 47 3033 663 2081 Fragments arranged by size: 3033 2081 663 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5824 StyI C'CwwG_G Cuts at: 513 1065 2691 3263 513 Size: 552 1626 572 3074 Fragments arranged by size: 3074 1626 572 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2024 3851 3868 4021 4206 4651 325 Size: 1255 444 1827 17 153 185 445 1498 Fragments arranged by size: 1827 1498 1255 445 444 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2268 2797 4070 Size: 274 80 33 51 1443 291 529 1273 Cuts at: 4070 5782 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1273 529 291 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2357 2389 2709 2741 5784 5816 2357 Size: 32 320 32 3043 32 2365 Fragments arranged by size: 3043 2365 320 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5824 XbaI T'CTAG_A Cuts at: 3032 3160 3032 Size: 128 5696 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5824 XmaI C'CCGG_G Cuts at: 1870 3149 1870 Size: 1279 4545 XmnI GAAnn'nnTTC Cuts at: 3953 3953 Size: 5824 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3632 276 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI RsrII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI