Restriction map for
pFN21AB9844
(Circular) (Six-base) MAPSORT of: pFN21AB9844.seq Check: 4240 from: 1 to: 5821
pFN21AB9844 5821 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3631 278
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3164 1937
Size: 1227 4594
Acc65I G'GTAC_C
Cuts at: 3142 3142
Size: 5821
AclI AA'CG_TT
Cuts at: 1563 3948 4321 1563
Size: 2385 373 3063
Fragments arranged by size:
3063 2385 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3884 5268 5536 1632
Size: 2252 1384 268 1917
Fragments arranged by size:
2252 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5624
AflIII A'CryG_T
Cuts at: 1203 4720 5697 1203
Size: 3517 977 1327
Fragments arranged by size:
3517 1327 977
AgeI A'CCGG_T
Cuts at: 4652 4652
Size: 5821
AhdI GACnn_n'nnGTC
Cuts at: 4550 4550
Size: 5821
AleI CACnn'nnGTG
Cuts at: 1166 2856 1166
Size: 1690 4131
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3014 1184
Size: 1830 3991
AlwNI CAG_nnn'CTG
Cuts at: 3564 5136 3564
Size: 1572 4249
ApaI G_GGCC'C
Cuts at: 2844 2844
Size: 5821
ApaLI G'TGCA_C
Cuts at: 2941 3878 5034 2941
Size: 937 1156 3728
Fragments arranged by size:
3728 1156 937
ApoI r'AATT_y
Cuts at: 1493 2714 3130 3356 3508 5617 1493
Size: 1221 416 226 152 2109 1697
Fragments arranged by size:
2109 1697 1221 416 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4375 160
Size: 872 3343 1606
Fragments arranged by size:
3343 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
AvaI C'yCGr_G
Cuts at: 1870 1943 3146 1870
Size: 73 1203 4545
Fragments arranged by size:
4545 1203 73
BamHI G'GATC_C
Cuts at: 3151 3518 3151
Size: 367 5454
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3142 4597 618
Size: 325 217 627 1355 1455 1842
Fragments arranged by size:
1842 1455 1355 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2070 2091 2844 2891 3140 1391
Size: 679 21 753 47 249 4072
Fragments arranged by size:
4072 753 679 249 47 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2346 3110 928
Size: 971 447 764 3639
Fragments arranged by size:
3639 971 764 447
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4012 4046 1903
Size: 34 2075 34 3678
Fragments arranged by size:
3678 2075 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3713 4923 3713
Size: 1210 4611
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2384 2474 4432
Size: 107 122 71 631 605 712 90 1958
Cuts at: 4432 136
Size: 1525
Fragments arranged by size:
1958 1525 712 631 605 122 107 90
71
BglII A'GATC_T
Cuts at: 5816 5816
Size: 5821
BlpI GC'TnA_GC
Cuts at: 3238 3238
Size: 5821
Bme1580I G_kGCm'C
Cuts at: 1790 2501 2844 2945 3035 3882 5038 1790
Size: 711 343 101 90 847 1156 2573
Fragments arranged by size:
2573 1156 847 711 343 101 90
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5821
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3552 4510 442
Size: 416 53 83 399 24 2135 958 1753
Fragments arranged by size:
2135 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 2473 2561 4481 1239
Size: 824 410 88 1920 2579
Fragments arranged by size:
2579 1920 824 410 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2903 3946 4811 5109 2903
Size: 1043 865 298 3615
Fragments arranged by size:
3615 1043 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4484 882
Size: 617 2985 2219
Fragments arranged by size:
2985 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2854 3612 493
Size: 711 1650 758 2702
Fragments arranged by size:
2702 1650 758 711
BsaBI GATnn'nnATC
Cuts at: 5815 5815
Size: 5821
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3628 4010 4645 275
Size: 53 83 186 936 2095 382 635 1451
Fragments arranged by size:
2095 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4254 4652 4926 5073 5484 4254
Size: 398 274 147 411 4591
Fragments arranged by size:
4591 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2300 2330 2534 2564 1178
Size: 30 165 30 897 30 204 30 4435
Fragments arranged by size:
4435 897 204 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5821
BseYI C'CCAG_C
Cuts at: 2426 2438 5024 5520 2426
Size: 12 2586 496 2727
Fragments arranged by size:
2727 2586 496 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2880 1163
Size: 573 1144 4104
Fragments arranged by size:
4104 1144 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3271 4032 5060 1540
Size: 275 186 1270 761 1028 2301
Fragments arranged by size:
2301 1270 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2070 2349 2891 2945 3882 3967 5038 2070
Size: 279 542 54 937 85 1071 2853
Fragments arranged by size:
2853 1071 937 542 279 85 54
BsmI GAATG_Cn'
Cuts at: 3332 3425 3332
Size: 93 5728
BsmBI CGTCTCn'nnnn_
Cuts at: 2893 2893
Size: 5821
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2091 2349 2501 2844 2891 2945
Size: 399 280 21 258 152 343 47 54
Cuts at: 2945 3035 3140 3882 3967 5038 1391
Size: 90 105 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 343 280 258 152
105 90 85 54 47 21
BspEI T'CCGG_A
Cuts at: 5484 5484
Size: 5821
BspHI T'CATG_A
Cuts at: 3710 5440 3710
Size: 1730 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2518 3176 844
Size: 1674 658 3489
Fragments arranged by size:
3489 1674 658
BsrBI CCG'CTC
Cuts at: 1394 1823 2197 3708 1394
Size: 429 374 1511 3507
Fragments arranged by size:
3507 1511 429 374
BsrDI GCAATG_nn'
Cuts at: 65 1248 4316 4490 65
Size: 1183 3068 174 1396
Fragments arranged by size:
3068 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2921 3011 4465 4652 1700
Size: 110 1111 90 1454 187 2869
Fragments arranged by size:
2869 1454 1111 187 110 90
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5821
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5821
BssSI C'ACGA_G
Cuts at: 2500 3881 4893 2500
Size: 1381 1012 3428
Fragments arranged by size:
3428 1381 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5821
BstBI TT'CG_AA
Cuts at: 2949 4625 2949
Size: 1676 4145
BstEII G'GTnAC_C
Cuts at: 1184 2673 1184
Size: 1489 4332
BstYI r'GATC_y
Cuts at: 1972 2046 2151 2695 3151 3518 3903 3920 5372
Size: 74 105 544 456 367 385 17 1452
Cuts at: 5372 5458 5470 5816 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 544 456 385 367 346 105
86 74 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2836 513
Size: 552 939 832 3498
Fragments arranged by size:
3498 939 832 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5082
Fragments arranged by size:
5082 683 56
BtsI GCAGTG_nn'
Cuts at: 2604 3337 4131 4151 2604
Size: 733 794 20 4274
Fragments arranged by size:
4274 794 733 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5821
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5786
DraI TTT'AAA
Cuts at: 3125 3125
Size: 5821
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2566 4828 809
Size: 507 1250 2262 1802
Fragments arranged by size:
2262 1802 1250 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2161 3268 4157
Size: 54 1425 50 275 18 331 1107 889
Cuts at: 4157 5786 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1107 889 331 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3268 1537
Size: 275 1456 4090
Fragments arranged by size:
4090 1456 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2204 3751 1353
Size: 851 1547 3423
Fragments arranged by size:
3423 1547 851
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2163 4408 4782 4928 387
Size: 1064 138 574 2245 374 146 1280
Fragments arranged by size:
2245 1280 1064 574 374 146 138
EcoICRI GAG'CTC
Cuts at: 2068 2889 2068
Size: 821 5000
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2473 2561 3884 4481 5268 5536
Size: 393 431 410 88 1323 597 787 268
Cuts at: 5536 1239
Size: 1524
Fragments arranged by size:
1524 1323 787 597 431 410 393 268
88
EcoRI G'AATT_C
Cuts at: 3130 3508 3130
Size: 378 5443
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2897 2897
Size: 5821
FspI TGC'GCA
Cuts at: 2990 3002 4327 2990
Size: 12 1325 4484
Fragments arranged by size:
4484 1325 12
HaeII r_GCGC'y
Cuts at: 1678 4968 1678
Size: 3290 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2809 2841 4462 4494 4536
Size: 32 135 32 1237 32 1621 32 42
Cuts at: 4536 4568 1373
Size: 32 2626
Fragments arranged by size:
2626 1621 1237 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3069 3165 3411 669
Size: 988 281 1131 96 246 3079
Fragments arranged by size:
3079 1131 988 281 246 96
HindIII A'AGCT_T
Cuts at: 748 2874 748
Size: 2126 3695
HpaI GTT'AAC
Cuts at: 3411 3411
Size: 5821
KpnI G_GTAC'C
Cuts at: 3146 3146
Size: 5821
MfeI C'AATT_G
Cuts at: 2146 3420 2146
Size: 1274 4547
MluI A'CGCG_T
Cuts at: 5697 5697
Size: 5821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2037 2135 4935 5119 1305
Size: 732 98 2800 184 2007
Fragments arranged by size:
2800 2007 732 184 98
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2163 10
Size: 54 1425 343 331 3668
Fragments arranged by size:
3668 1425 343 331 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2081 2856 3779 4138 4297 518
Size: 648 915 775 923 359 159 2042
Fragments arranged by size:
2042 923 915 775 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2442 2707 2911 3237 3914 5062 5307 1606
Size: 836 265 204 326 677 1148 245 2120
Fragments arranged by size:
2120 1148 836 677 326 265 245 204
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4330
Fragments arranged by size:
4330 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5821
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5821
NotI GC'GGCC_GC
Cuts at: 3268 3268
Size: 5821
NspI r_CATG'y
Cuts at: 1132 1229 3101 3179 4724 1132
Size: 97 1872 78 1545 2229
Fragments arranged by size:
2229 1872 1545 97 78
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5821
PciI A'CATG_T
Cuts at: 4720 4720
Size: 5821
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5821
PfoI T'CCnGG_A
Cuts at: 2325 2799 2325
Size: 474 5347
PmeI GTTT'AAAC
Cuts at: 3125 3125
Size: 5821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 5403 5435 3890
Size: 32 1481 32 4276
Fragments arranged by size:
4276 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5821
PsiI TTA'TAA
Cuts at: 3391 3391
Size: 5821
PspOMI G'GGCC_C
Cuts at: 2840 2840
Size: 5821
PstI C_TGCA'G
Cuts at: 830 3173 830
Size: 2343 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5821
PvuII CAG'CTG
Cuts at: 2707 2911 2707
Size: 204 5617
SacI G_AGCT'C
Cuts at: 2070 2891 2070
Size: 821 5000
SalI G'TCGA_C
Cuts at: 1936 3163 1936
Size: 1227 4594
SapI GCTCTTCn'nnn_
Cuts at: 2204 2204
Size: 5821
SbfI CC_TGCA'GG
Cuts at: 3173 3173
Size: 5821
ScaI AGT'ACT
Cuts at: 2642 4069 5781 2642
Size: 1427 1712 2682
Fragments arranged by size:
2682 1712 1427
SfcI C'TryA_G
Cuts at: 826 1045 3169 4304 4985 5176 826
Size: 219 2124 1135 681 191 1471
Fragments arranged by size:
2124 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
SgrAI Cr'CCGG_yG
Cuts at: 1700 2921 3011 1700
Size: 1221 90 4510
Fragments arranged by size:
4510 1221 90
SmaI CCC'GGG
Cuts at: 1872 3148 1872
Size: 1276 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2882 3925 4826 5088 820
Size: 197 926 939 1043 901 262 1553
Fragments arranged by size:
1553 1043 939 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3612 493
Size: 3119 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5821
SphI G_CATG'C
Cuts at: 1132 3179 1132
Size: 2047 3774
SspI AAT'ATT
Cuts at: 5 52 3745 5
Size: 47 3693 2081
Fragments arranged by size:
3693 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5821
StyI C'CwwG_G
Cuts at: 513 1065 2004 2590 3260 513
Size: 552 939 586 670 3074
Fragments arranged by size:
3074 939 670 586 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2591 3848 3865 4018 4203 4648 325
Size: 1255 1011 1257 17 153 185 445 1498
Fragments arranged by size:
1498 1257 1255 1011 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2640 4067 5779
Size: 274 80 33 51 1443 663 1427 1712
Cuts at: 5779 96
Size: 138
Fragments arranged by size:
1712 1443 1427 663 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2143 2175 5781 5813 2143
Size: 32 3606 32 2151
Fragments arranged by size:
3606 2151 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5821
XbaI T'CTAG_A
Cuts at: 3157 3157
Size: 5821
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5821
XmaI C'CCGG_G
Cuts at: 1870 3146 1870
Size: 1276 4545
XmnI GAAnn'nnTTC
Cuts at: 3950 3950
Size: 5821
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3629 276
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI