Restriction map for
pFN21AB9841
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# NO CUT ENZYMES ( 129 enzymes) #
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Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AvrII BamHI BbeI BbrPI BbvCI
BcgI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BseAI BsiHKAI BsiWI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BssHII Bst1107I
BstBI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraII EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoRI EcoRV EcoT22I FauI FbaI
FseI HaeII HgaI HindIII HpaI
KasI KpnI MaeI MamI MfeI
MflI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI SfoI SfuI SgrAI
SmaI SmlI SpeI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 1025 GACGTC GACGT^C
AccII 722 CGCG CG^CG
AcyI 1025 GACGTC G[GA]^CG[CT]C
AlwI 474 GATCC GGATC(4/5)
AocI 961 CCTGAGG CC^T[ACGT]AGG
Asp700I 612 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 348 GGTCC G^G[AT]CC
AviII 299 TGCGCA TGC^GCA
BalI 951 TGGCCA TGG^CCA
BanI 966 GGTGCC G^G[CT][GA]CC
BanII 354 GGGCTC G[GA]GC[CT]^C
BciVI 592 GTATCC GTATCC(6/5)
BglI 331 GCCGAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 589 TACGTA [CT]AC^GT[GA]
BsaHI 1025 GACGTC G[GA]^CG[CT]C
BsaWI 155 TCCGGT [AT]^CCGG[AT]
BseRI 655 CTCCTC GAGGAG(10/8)
BsiEI 53 CGACCG CG[GA][CT]^CG
BsmBI 252 CGTCTC CGTCTC(1/5)
Bsp1407I 272 TGTACA T^GTACA
BsrGI 272 TGTACA T^GTACA
BssSI 103 CTCGTG CACGAG(-5/-1)
BstAPI 291 GCACACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 722 CGCG CG^CG
Bsu36I 961 CCTGAGG CC^T[ACGT]AGG
DraIII 292 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 880 GACAACCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 951 TGGCCA [CT]^GGCC[GA]
EarI 85 CTCTTC CTCTTC(1/4)
Eco81I 961 CCTGAGG CC^T[ACGT]AGG
EcoNI 922 CCTGGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 299 TGCGCA TGC^GCA
Hin1I 1025 GACGTC G[GA]^CG[CT]C
HincII 580 GTCAAC GT[CT]^[GA]AC
HindII 580 GTCAAC GT[CT]^[GA]AC
Ksp632I 85 CTCTTC CTCTTC(1/4)
KspI 721 CCGCGG CCGC^GG
MluNI 951 TGGCCA TGG^CCA
MscI 951 TGGCCA TGG^CCA
MseI 800 TTAA T^TAA
MvnI 722 CGCG CG^CG
PstI 238 CTGCAG CTGCA^G
PvuII 116 CAGCTG CAG^CTG
SacII 721 CCGCGG CCGC^GG
ScaI 512 AGTACT AGT^ACT
SfaNI 467 GCATC GCATC(5/9)
SfcI 238 CTGCAG C^T[GA][CT]AG
SfiI 330 GGCCGAGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 589 TACGTA TAC^GTA
SphI 302 GCATGC GCATG^C
SspBI 272 TGTACA T^GTACA
TaqI 52 TCGA T^CGA
Tru9I 800 TTAA T^TAA
TthHB8I 52 TCGA T^CGA
XmnI 612 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcsI 24 AAATTT [GA]^AATT[CT]
832 AAATTC
AlwNI 116 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
805 CAGTGCCTG
ApoI 24 AAATTT [GA]^AATT[CT]
832 AAATTC
BbsI 126 GTCTTC GAAGAC(2/6)
616 GTCTTC
BpuAI 126 GTCTTC GAAGAC(2/6)
616 GTCTTC
BsmAI 253 GTCTC GTCTC(1/5)
979 GTCTC
BsrI 230 ACTGG ACTGG(1/-1)
510 CCAGT
BstEII 888 GGTCACC G^GT[ACGT]ACC
988 GGTCACC
BstPI 888 GGTCACC G^GT[ACGT]ACC
988 GGTCACC
BstXI 485 CCATGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
744 CCAGCTTTCTGG
CfoI 300 GCGC GCG^C
315 GCGC
EcoO65I 888 GGTCACC G^GT[ACGT]ACC
988 GGTCACC
EcoT14I 485 CCATGG C^C[AT][AT]GG
1002 CCATGG
HhaI 300 GCGC GCG^C
315 GCGC
HinP1I 300 GCGC G^CGC
315 GCGC
MaeII 590 ACGT A^CGT
1026 ACGT
MspA1I 116 CAGCTG C[AC]G^C[GT]G
721 CCGCGG
NcoI 485 CCATGG C^CATGG
1002 CCATGG
NlaIV 754 GGCCCC GG[ACGT]^[ACGT]CC
966 GGTGCC
NspI 302 GCATGC [GA]CATG^[CT]
546 GCATGT
SexAI 884 ACCTGGT A^CC[AT]GGT
984 ACCAGGT
StyI 485 CCATGG C^C[AT][AT]GG
1002 CCATGG
TfiI 168 GATTC G^A[AT]TC
527 GAATC
Tsp509I 25 AATT ^AATT
833 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI BbvI BcnI
BmyI BpmI BsaJI BsgI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DsaI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeIII MboI MboII MnlI MslI
MspI MvaI MwoI NciI NdeII
NlaIII PleI RsaI Sau3AI Sau96I
ScrFI TseI Tsp45I TspRI XcmI