(Circular) (Six-base) MAPSORT of: pFN21AB9841.seq Check: 9730 from: 1 to: 5779 pFN21AB9841 5779 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3034 3589 278 Size: 53 83 186 936 1498 555 2468 Fragments arranged by size: 2468 1498 936 555 186 83 53 AccI GT'mk_AC Cuts at: 1937 3122 1937 Size: 1185 4594 Acc65I G'GTAC_C Cuts at: 3100 3100 Size: 5779 AclI AA'CG_TT Cuts at: 1563 3906 4279 1563 Size: 2343 373 3063 Fragments arranged by size: 3063 2343 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2836 3842 5226 5494 1632 Size: 1204 1006 1384 268 1917 Fragments arranged by size: 1917 1384 1204 1006 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5582 AflIII A'CryG_T Cuts at: 1203 4678 5655 1203 Size: 3475 977 1327 Fragments arranged by size: 3475 1327 977 AgeI A'CCGG_T Cuts at: 4610 4610 Size: 5779 AhdI GACnn_n'nnGTC Cuts at: 4508 4508 Size: 5779 AleI CACnn'nnGTG Cuts at: 1166 2462 1166 Size: 1296 4483 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5779 AlwNI CAG_nnn'CTG Cuts at: 2126 2815 3522 5094 2126 Size: 689 707 1572 2811 Fragments arranged by size: 2811 1572 707 689 ApaLI G'TGCA_C Cuts at: 3836 4992 3836 Size: 1156 4623 ApoI r'AATT_y Cuts at: 1493 2029 2837 3088 3314 3466 5575 1493 Size: 536 808 251 226 152 2109 1697 Fragments arranged by size: 2109 1697 808 536 251 226 152 AseI AT'TA_AT Cuts at: 160 1032 4333 160 Size: 872 3301 1606 Fragments arranged by size: 3301 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5779 AvaI C'yCGr_G Cuts at: 1870 1943 3104 1870 Size: 73 1161 4545 Fragments arranged by size: 4545 1161 73 BamHI G'GATC_C Cuts at: 3109 3476 3109 Size: 367 5412 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2971 3100 4555 618 Size: 325 217 627 1184 129 1455 1842 Fragments arranged by size: 1842 1455 1184 627 325 217 129 BanII G_rGCy'C Cuts at: 1391 2363 3098 1391 Size: 972 735 4072 Fragments arranged by size: 4072 972 735 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2124 2614 928 Size: 971 225 490 4093 Fragments arranged by size: 4093 971 490 225 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3970 4004 1903 Size: 34 2033 34 3678 Fragments arranged by size: 3678 2033 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2608 3671 4881 2608 Size: 1063 1210 3506 Fragments arranged by size: 3506 1210 1063 BclI T'GATC_A Cuts at: 1548 1548 Size: 5779 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2342 4390 136 Size: 107 122 71 631 605 670 2048 1525 Fragments arranged by size: 2048 1525 670 631 605 122 107 71 BglII A'GATC_T Cuts at: 5774 5774 Size: 5779 BlpI GC'TnA_GC Cuts at: 3196 3196 Size: 5779 Bme1580I G_kGCm'C Cuts at: 1790 2120 2447 2976 3840 4996 1790 Size: 330 327 529 864 1156 2573 Fragments arranged by size: 2573 1156 864 529 330 327 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5779 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2245 3510 4468 Size: 416 53 83 399 24 828 1265 958 Cuts at: 4468 442 Size: 1753 Fragments arranged by size: 1753 1265 958 828 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5779 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2057 2423 2837 4439 1239 Size: 818 366 414 1602 2579 Fragments arranged by size: 2579 1602 818 414 366 Bpu10I CC'TnA_GC Cuts at: 2666 2666 Size: 5779 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3904 4769 5067 3904 Size: 865 298 4616 Fragments arranged by size: 4616 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4442 882 Size: 617 2943 2219 Fragments arranged by size: 2943 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2596 3570 493 Size: 711 1392 974 2702 Fragments arranged by size: 2702 1392 974 711 BsaBI GATnn'nnATC Cuts at: 5773 5773 Size: 5779 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3031 3586 3968 4603 Size: 53 83 186 936 1498 555 382 635 Cuts at: 4603 275 Size: 1451 Fragments arranged by size: 1498 1451 936 635 555 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2160 4212 4610 4884 5031 5442 2160 Size: 2052 398 274 147 411 2497 Fragments arranged by size: 2497 2052 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2557 2587 2643 2673 1178 Size: 30 165 30 1154 30 56 30 4284 Fragments arranged by size: 4284 1154 165 56 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2651 1186 Size: 1465 4314 BseYI C'CCAG_C Cuts at: 2113 2409 4982 5478 2113 Size: 296 2573 496 2414 Fragments arranged by size: 2573 2414 496 296 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2488 2634 2770 1163 Size: 573 752 146 136 4172 Fragments arranged by size: 4172 752 573 146 136 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2061 3229 3990 5018 1540 Size: 275 186 60 1168 761 1028 2301 Fragments arranged by size: 2301 1168 1028 761 275 186 60 BsiHKAI G_wGCw'C Cuts at: 3840 3925 4996 3840 Size: 85 1071 4623 Fragments arranged by size: 4623 1071 85 BsmI GAATG_Cn' Cuts at: 3290 3383 3290 Size: 93 5686 BsmBI CGTCTCn'nnnn_ Cuts at: 2263 2263 Size: 5779 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2120 2363 2447 2976 3098 3840 3925 Size: 399 330 243 84 529 122 742 85 Cuts at: 3925 4996 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 529 399 330 243 122 85 84 BspEI T'CCGG_A Cuts at: 5442 5442 Size: 5779 BspHI T'CATG_A Cuts at: 3668 5398 3668 Size: 1730 4049 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3134 844 Size: 2290 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3666 1394 Size: 429 1843 3507 Fragments arranged by size: 3507 1843 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4274 4448 65 Size: 1183 3026 174 1396 Fragments arranged by size: 3026 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4423 4610 1700 Size: 110 2613 187 2869 Fragments arranged by size: 2869 2613 187 110 BsrGI T'GTAC_A Cuts at: 96 2277 96 Size: 2181 3598 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5779 BssSI C'ACGA_G Cuts at: 2108 3839 4851 2108 Size: 1731 1012 3036 Fragments arranged by size: 3036 1731 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2302 794 Size: 1508 4271 BstBI TT'CG_AA Cuts at: 4583 4583 Size: 5779 BstEII G'GTnAC_C Cuts at: 1184 2893 2993 1184 Size: 1709 100 3970 Fragments arranged by size: 3970 1709 100 BstXI CCAn_nnnn'nTGG Cuts at: 2497 2756 2497 Size: 259 5520 BstYI r'GATC_y Cuts at: 1972 3109 3476 3861 3878 5330 5416 5428 5774 Size: 1137 367 385 17 1452 86 12 346 Cuts at: 5774 1972 Size: 1977 Fragments arranged by size: 1977 1452 1137 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2967 2967 Size: 5779 BtgI C'CryG_G Cuts at: 513 1065 2004 2061 2490 2726 3007 513 Size: 552 939 57 429 236 281 3285 Fragments arranged by size: 3285 939 552 429 281 236 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5040 Fragments arranged by size: 5040 683 56 BtsI GCAGTG_nn' Cuts at: 3295 4089 4109 3295 Size: 794 20 4965 Fragments arranged by size: 4965 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5779 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5744 DraI TTT'AAA Cuts at: 3083 3083 Size: 5779 DraIII CAC_nnn'GTG Cuts at: 2302 2302 Size: 5779 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2891 4786 809 Size: 507 1575 1895 1802 Fragments arranged by size: 1895 1802 1575 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2956 3226 4115 Size: 54 1425 50 275 18 1126 270 889 Cuts at: 4115 5744 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1126 889 275 270 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3226 1537 Size: 275 1414 4090 Fragments arranged by size: 4090 1414 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2096 3709 1353 Size: 743 1613 3423 Fragments arranged by size: 3423 1613 743 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4366 4740 4886 387 Size: 1064 138 2777 374 146 1280 Fragments arranged by size: 2777 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2423 2836 2837 3842 4439 5226 Size: 393 425 366 413 1 1005 597 787 Cuts at: 5226 5494 1239 Size: 268 1524 Fragments arranged by size: 1524 1005 787 597 425 413 393 366 268 1 EcoNI CCTnn'n_nnAGG Cuts at: 2931 2931 Size: 5779 EcoRI G'AATT_C Cuts at: 3088 3466 3088 Size: 378 5401 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2638 2638 Size: 5779 FspI TGC'GCA Cuts at: 2306 4285 2306 Size: 1979 3800 FspAI rTGC'GCAy Cuts at: 2306 2306 Size: 5779 HaeII r_GCGC'y Cuts at: 1678 4926 1678 Size: 3248 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2557 2589 3056 3088 4420 Size: 32 135 32 985 32 467 32 1332 Cuts at: 4420 4452 4494 4526 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1332 985 467 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2587 3123 3369 669 Size: 988 281 649 536 246 3079 Fragments arranged by size: 3079 988 649 536 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5779 HpaI GTT'AAC Cuts at: 3369 3369 Size: 5779 KpnI G_GTAC'C Cuts at: 3104 3104 Size: 5779 MfeI C'AATT_G Cuts at: 3378 3378 Size: 5779 MluI A'CGCG_T Cuts at: 5655 5655 Size: 5779 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4893 5077 1305 Size: 3588 184 2007 Fragments arranged by size: 3588 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2958 10 Size: 54 1425 343 1126 2831 Fragments arranged by size: 2831 1425 1126 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2462 2489 2495 3737 4096 4255 518 Size: 648 1296 27 6 1242 359 159 2042 Fragments arranged by size: 2042 1296 1242 648 359 159 27 6 MspA1I CmG'CkG Cuts at: 1606 2123 2728 3195 3872 5020 5265 1606 Size: 517 605 467 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 605 517 467 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2490 3007 513 Size: 552 939 486 517 3285 Fragments arranged by size: 3285 939 552 517 486 NdeI CA'TA_TG Cuts at: 387 387 Size: 5779 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5779 NotI GC'GGCC_GC Cuts at: 3226 3226 Size: 5779 NspI r_CATG'y Cuts at: 1132 1229 2311 2555 3137 4682 1132 Size: 97 1082 244 582 1545 2229 Fragments arranged by size: 2229 1545 1082 582 244 97 PasI CC'CwG_GG Cuts at: 1348 2828 1348 Size: 1480 4299 PciI A'CATG_T Cuts at: 4678 4678 Size: 5779 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5779 PfoI T'CCnGG_A Cuts at: 2034 2034 Size: 5779 PmeI GTTT'AAAC Cuts at: 3083 3083 Size: 5779 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3848 3880 5361 5393 3848 Size: 32 1481 32 4234 Fragments arranged by size: 4234 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5779 PsiI TTA'TAA Cuts at: 3349 3349 Size: 5779 PstI C_TGCA'G Cuts at: 830 2247 3131 830 Size: 1417 884 3478 Fragments arranged by size: 3478 1417 884 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5779 PvuII CAG'CTG Cuts at: 2123 2123 Size: 5779 SacII CC_GC'GG Cuts at: 2729 2729 Size: 5779 SalI G'TCGA_C Cuts at: 1936 3121 1936 Size: 1185 4594 SbfI CC_TGCA'GG Cuts at: 3131 3131 Size: 5779 ScaI AGT'ACT Cuts at: 2519 4027 5739 2519 Size: 1508 1712 2559 Fragments arranged by size: 2559 1712 1508 SexAI A'CCwGG_T Cuts at: 2889 2989 2889 Size: 100 5679 SfcI C'TryA_G Cuts at: 826 1045 2243 3127 4262 4943 5134 826 Size: 219 1198 884 1135 681 191 1471 Fragments arranged by size: 1471 1198 1135 884 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2342 2342 Size: 5779 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5779 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5779 SmaI CCC'GGG Cuts at: 1872 3106 1872 Size: 1234 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3883 4784 5046 820 Size: 197 926 1940 901 262 1553 Fragments arranged by size: 1940 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2596 3570 493 Size: 2103 974 2702 Fragments arranged by size: 2702 2103 974 SpeI A'CTAG_T Cuts at: 152 152 Size: 5779 SphI G_CATG'C Cuts at: 1132 2311 3137 1132 Size: 1179 826 3774 Fragments arranged by size: 3774 1179 826 SspI AAT'ATT Cuts at: 5 52 3703 5 Size: 47 3651 2081 Fragments arranged by size: 3651 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5779 StyI C'CwwG_G Cuts at: 513 1065 2004 2490 3007 3218 513 Size: 552 939 486 517 211 3074 Fragments arranged by size: 3074 939 552 517 486 211 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2227 3806 3823 3976 4161 4606 325 Size: 1255 647 1579 17 153 185 445 1498 Fragments arranged by size: 1579 1498 1255 647 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2044 2277 2517 Size: 274 80 33 51 1443 67 233 240 Cuts at: 2517 4025 5737 96 Size: 1508 1712 138 Fragments arranged by size: 1712 1508 1443 274 240 233 138 80 67 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2643 2675 5739 5771 2643 Size: 32 3064 32 2651 Fragments arranged by size: 3064 2651 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5779 XbaI T'CTAG_A Cuts at: 3115 3115 Size: 5779 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2269 2494 2951 2269 Size: 225 457 5097 Fragments arranged by size: 5097 457 225 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5779 XmaI C'CCGG_G Cuts at: 1870 3104 1870 Size: 1234 4545 XmnI GAAnn'nnTTC Cuts at: 2621 3908 2621 Size: 1287 4492 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3032 3587 276 Size: 53 83 186 936 1498 555 2468 Fragments arranged by size: 2468 1498 936 555 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I EcoICRI EcoO109I EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SfoI SrfI SwaI