Restriction map for
pFN21AB9841
(Linear) (Six-base) MAPSORT of: KIBB9841.seq Check: 1822 from: 1 to: 1074
KIBB9841.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AatII G_ACGT'C
Cuts at: 0 1029 1074
Size: 1029 45
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 831 1074
Size: 831 243
AleI CACnn'nnGTG
Cuts at: 0 457 1074
Size: 457 617
AlwNI CAG_nnn'CTG
Cuts at: 0 121 810 1074
Size: 121 689 264
Fragments arranged by size:
689 264 121
ApoI r'AATT_y
Cuts at: 0 24 832 1074
Size: 24 808 242
Fragments arranged by size:
808 242 24
BanI G'GyrC_C
Cuts at: 0 966 1074
Size: 966 108
BanII G_rGCy'C
Cuts at: 0 358 1074
Size: 358 716
BbsI GAAGACnn'nnnn_
Cuts at: 0 119 609 1074
Size: 119 490 465
Fragments arranged by size:
490 465 119
BciVI GTATCCnnnnn_n'
Cuts at: 0 603 1074
Size: 603 471
BglI GCCn_nnn'nGGC
Cuts at: 0 337 1074
Size: 337 737
Bme1580I G_kGCm'C
Cuts at: 0 115 442 971 1074
Size: 115 327 529 103
Fragments arranged by size:
529 327 115 103
BmrI ACTGGGnnnn_n'
Cuts at: 0 240 1074
Size: 240 834
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 418 832 1074
Size: 52 366 414 242
Fragments arranged by size:
414 366 242 52
Bpu10I CC'TnA_GC
Cuts at: 0 661 1074
Size: 661 413
BsaAI yAC'GTr
Cuts at: 0 591 1074
Size: 591 483
BsaHI Gr'CG_yC
Cuts at: 0 1026 1074
Size: 1026 48
BsaWI w'CCGG_w
Cuts at: 0 155 1074
Size: 155 919
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 552 582 638 668 1074
Size: 552 30 56 30 406
Fragments arranged by size:
552 406 56 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 646 1074
Size: 646 428
BseYI C'CCAG_C
Cuts at: 0 108 404 1074
Size: 108 296 670
Fragments arranged by size:
670 296 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 483 629 765 1074
Size: 483 146 136 309
Fragments arranged by size:
483 309 146 136
BsiEI CG_ry'CG
Cuts at: 0 56 1074
Size: 56 1018
BsmBI CGTCTCn'nnnn_
Cuts at: 0 258 1074
Size: 258 816
Bsp1286I G_dGCh'C
Cuts at: 0 115 358 442 971 1074
Size: 115 243 84 529 103
Fragments arranged by size:
529 243 115 103 84
BsrGI T'GTAC_A
Cuts at: 0 272 1074
Size: 272 802
BssSI C'ACGA_G
Cuts at: 0 103 1074
Size: 103 971
BstAPI GCAn_nnn'nTGC
Cuts at: 0 297 1074
Size: 297 777
BstEII G'GTnAC_C
Cuts at: 0 888 988 1074
Size: 888 100 86
Fragments arranged by size:
888 100 86
BstXI CCAn_nnnn'nTGG
Cuts at: 0 492 751 1074
Size: 492 259 323
Fragments arranged by size:
492 323 259
Bsu36I CC'TnA_GG
Cuts at: 0 962 1074
Size: 962 112
BtgI C'CryG_G
Cuts at: 0 56 485 721 1002 1074
Size: 56 429 236 281 72
Fragments arranged by size:
429 281 236 72 56
DraIII CAC_nnn'GTG
Cuts at: 0 297 1074
Size: 297 777
DrdI GACnn_nn'nnGTC
Cuts at: 0 886 1074
Size: 886 188
EaeI y'GGCC_r
Cuts at: 0 951 1074
Size: 951 123
EarI CTCTTCn'nnn_
Cuts at: 0 91 1074
Size: 91 983
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 418 831 832 1074
Size: 52 366 413 1 242
Fragments arranged by size:
413 366 242 52 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 926 1074
Size: 926 148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 633 1074
Size: 633 441
FspI TGC'GCA
Cuts at: 0 301 1074
Size: 301 773
FspAI rTGC'GCAy
Cuts at: 0 301 1074
Size: 301 773
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 552 584 1051 1074
Size: 552 32 467 23
Fragments arranged by size:
552 467 32 23
HincII GTy'rAC
Cuts at: 0 582 1074
Size: 582 492
MscI TGG'CCA
Cuts at: 0 953 1074
Size: 953 121
MslI CAynn'nnrTG
Cuts at: 0 457 484 490 1074
Size: 457 27 6 584
Fragments arranged by size:
584 457 27 6
MspA1I CmG'CkG
Cuts at: 0 118 723 1074
Size: 118 605 351
Fragments arranged by size:
605 351 118
NcoI C'CATG_G
Cuts at: 0 485 1002 1074
Size: 485 517 72
Fragments arranged by size:
517 485 72
NspI r_CATG'y
Cuts at: 0 306 550 1074
Size: 306 244 524
Fragments arranged by size:
524 306 244
PasI CC'CwG_GG
Cuts at: 0 823 1074
Size: 823 251
PfoI T'CCnGG_A
Cuts at: 0 29 1074
Size: 29 1045
PstI C_TGCA'G
Cuts at: 0 242 1074
Size: 242 832
PvuII CAG'CTG
Cuts at: 0 118 1074
Size: 118 956
SacII CC_GC'GG
Cuts at: 0 724 1074
Size: 724 350
ScaI AGT'ACT
Cuts at: 0 514 1074
Size: 514 560
SexAI A'CCwGG_T
Cuts at: 0 884 984 1074
Size: 884 100 90
Fragments arranged by size:
884 100 90
SfcI C'TryA_G
Cuts at: 0 238 1074
Size: 238 836
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 337 1074
Size: 337 737
SnaBI TAC'GTA
Cuts at: 0 591 1074
Size: 591 483
SphI G_CATG'C
Cuts at: 0 306 1074
Size: 306 768
StyI C'CwwG_G
Cuts at: 0 485 1002 1074
Size: 485 517 72
Fragments arranged by size:
517 485 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 222 1074
Size: 222 852
TatI w'GTAC_w
Cuts at: 0 39 272 512 1074
Size: 39 233 240 562
Fragments arranged by size:
562 240 233 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 638 670 1074
Size: 638 32 404
Fragments arranged by size:
638 404 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 264 489 946 1074
Size: 264 225 457 128
Fragments arranged by size:
457 264 225 128
XmnI GAAnn'nnTTC
Cuts at: 0 616 1074
Size: 616 458
ZraI GAC'GTC
Cuts at: 0 1027 1074
Size: 1027 47
Enzymes that do cut:
AatII AcuI AleI AlwNI ApoI BanI BanII BbsI
BciVI BglI Bme1580I BmrI BpmI Bpu10I BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I
BsrGI BssSI BstAPI BstEII BstXI Bsu36I BtgI DraIII
DrdI EaeI EarI Eco57MI EcoNI FalI FspI FspAI
Hin4I HincII MscI MslI MspA1I NcoI NspI PasI
PfoI PstI PvuII SacII ScaI SexAI SfcI SfiI
SnaBI SphI StyI TaqII TatI TstI XcmI XmnI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BclI
BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaI
BsaBI BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BstBI BstYI BstZ17I BtgZI BtsI
ClaI CspCI DraI EagI EciI EcoICRI EcoO109I EcoRI
EcoRV FseI HaeII HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SalI SanDI SapI SbfI SfoI SgfI SgrAI
SmaI SmlI SpeI SrfI SspI StuI SwaI Tth111I
XbaI XhoI XmaI