(Circular) (Six-base) MAPSORT of: pFN21AB9837.seq Check: 4783 from: 1 to: 5761 pFN21AB9837 5761 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2543 2833 2543 Size: 290 5471 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3571 278 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3104 1937 Size: 1167 4594 Acc65I G'GTAC_C Cuts at: 2382 3082 2382 Size: 700 5061 AclI AA'CG_TT Cuts at: 1563 3888 4261 1563 Size: 2325 373 3063 Fragments arranged by size: 3063 2325 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2207 3824 5208 5476 1632 Size: 575 1617 1384 268 1917 Fragments arranged by size: 1917 1617 1384 575 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5564 AflIII A'CryG_T Cuts at: 1203 4660 5637 1203 Size: 3457 977 1327 Fragments arranged by size: 3457 1327 977 AgeI A'CCGG_T Cuts at: 4592 4592 Size: 5761 AhdI GACnn_n'nnGTC Cuts at: 4490 4490 Size: 5761 AleI CACnn'nnGTG Cuts at: 1166 2462 1166 Size: 1296 4465 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5761 AlwNI CAG_nnn'CTG Cuts at: 3504 5076 3504 Size: 1572 4189 ApaLI G'TGCA_C Cuts at: 3818 4974 3818 Size: 1156 4605 ApoI r'AATT_y Cuts at: 1493 2578 2789 3070 3296 3448 5557 1493 Size: 1085 211 281 226 152 2109 1697 Fragments arranged by size: 2109 1697 1085 281 226 211 152 AseI AT'TA_AT Cuts at: 160 1032 4315 160 Size: 872 3283 1606 Fragments arranged by size: 3283 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 AvaI C'yCGr_G Cuts at: 1870 1943 2647 2999 3086 1870 Size: 73 704 352 87 4545 Fragments arranged by size: 4545 704 352 87 73 BamHI G'GATC_C Cuts at: 3091 3458 3091 Size: 367 5394 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2382 3082 4537 618 Size: 325 217 627 595 700 1455 1842 Fragments arranged by size: 1842 1455 700 627 595 325 217 BanII G_rGCy'C Cuts at: 1391 2061 2266 2325 2752 3080 1391 Size: 670 205 59 427 328 4072 Fragments arranged by size: 4072 670 427 328 205 59 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2516 2926 928 Size: 971 617 410 3763 Fragments arranged by size: 3763 971 617 410 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3952 3986 1903 Size: 34 2015 34 3678 Fragments arranged by size: 3678 2015 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3653 4863 3653 Size: 1210 4551 BclI T'GATC_A Cuts at: 1548 2454 1548 Size: 906 4855 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4372 136 Size: 107 122 71 631 605 2700 1525 Fragments arranged by size: 2700 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2507 5756 2507 Size: 3249 2512 BlpI GC'TnA_GC Cuts at: 3178 3178 Size: 5761 Bme1580I G_kGCm'C Cuts at: 1790 3822 4978 1790 Size: 2032 1156 2573 Fragments arranged by size: 2573 2032 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5761 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2275 3042 3492 Size: 416 53 83 399 24 858 767 450 Cuts at: 3492 4450 442 Size: 958 1753 Fragments arranged by size: 1753 958 858 767 450 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5761 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4421 1239 Size: 3182 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3886 4751 5049 3886 Size: 865 298 4598 Fragments arranged by size: 4598 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4424 882 Size: 617 2925 2219 Fragments arranged by size: 2925 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3552 493 Size: 711 2348 2702 Fragments arranged by size: 2702 2348 711 BsaBI GATnn'nnATC Cuts at: 2600 5755 2600 Size: 3155 2606 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3568 3950 4585 275 Size: 53 83 186 936 2035 382 635 1451 Fragments arranged by size: 2035 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4194 4592 4866 5013 5424 4194 Size: 398 274 147 411 4531 Fragments arranged by size: 4531 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5536 Fragments arranged by size: 5536 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5761 BseYI C'CCAG_C Cuts at: 4964 5460 4964 Size: 496 5265 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5188 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3211 3972 5000 1540 Size: 275 186 1210 761 1028 2301 Fragments arranged by size: 2301 1210 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3822 3907 4978 3822 Size: 85 1071 4605 Fragments arranged by size: 4605 1071 85 BsmI GAATG_Cn' Cuts at: 3272 3365 3272 Size: 93 5668 BsmBI CGTCTCn'nnnn_ Cuts at: 2487 2487 Size: 5761 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2266 2325 2752 3080 3822 3907 Size: 399 271 205 59 427 328 742 85 Cuts at: 3907 4978 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 427 399 328 271 205 85 59 BspEI T'CCGG_A Cuts at: 5424 5424 Size: 5761 BspHI T'CATG_A Cuts at: 2724 3650 5380 2724 Size: 926 1730 3105 Fragments arranged by size: 3105 1730 926 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2217 2543 2833 3116 844 Size: 1373 326 290 283 3489 Fragments arranged by size: 3489 1373 326 290 283 BsrBI CCG'CTC Cuts at: 1394 1823 2110 3648 1394 Size: 429 287 1538 3507 Fragments arranged by size: 3507 1538 429 287 BsrDI GCAATG_nn' Cuts at: 65 1248 2949 4256 4430 65 Size: 1183 1701 1307 174 1396 Fragments arranged by size: 1701 1396 1307 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4405 4592 1700 Size: 110 2595 187 2869 Fragments arranged by size: 2869 2595 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5761 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5761 BssSI C'ACGA_G Cuts at: 3821 4833 3821 Size: 1012 4749 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5761 BstBI TT'CG_AA Cuts at: 4565 4565 Size: 5761 BstEII G'GTnAC_C Cuts at: 1184 2428 1184 Size: 1244 4517 BstYI r'GATC_y Cuts at: 1972 2507 3091 3458 3843 3860 5312 5398 5410 Size: 535 584 367 385 17 1452 86 12 Cuts at: 5410 5756 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 584 535 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4270 Fragments arranged by size: 4270 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5022 Fragments arranged by size: 5022 683 56 BtsI GCAGTG_nn' Cuts at: 2869 3277 4071 4091 2869 Size: 408 794 20 4539 Fragments arranged by size: 4539 794 408 20 ClaI AT'CG_AT Cuts at: 1554 2376 1554 Size: 822 4939 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5726 DraI TTT'AAA Cuts at: 2415 3065 2415 Size: 650 5111 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4768 809 Size: 507 3452 1802 Fragments arranged by size: 3452 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2220 3208 4097 Size: 54 1425 50 275 18 390 988 889 Cuts at: 4097 5726 8 Size: 1629 43 Fragments arranged by size: 1629 1425 988 889 390 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3208 1537 Size: 275 1396 4090 Fragments arranged by size: 4090 1396 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2183 2684 2747 3691 1353 Size: 830 501 63 944 3423 Fragments arranged by size: 3423 944 830 501 63 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4348 4722 4868 387 Size: 1064 138 2759 374 146 1280 Fragments arranged by size: 2759 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 3824 4421 5208 5476 1239 Size: 393 575 1617 597 787 268 1524 Fragments arranged by size: 1617 1524 787 597 575 393 268 EcoRI G'AATT_C Cuts at: 2789 3070 3448 2789 Size: 281 378 5102 Fragments arranged by size: 5102 378 281 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2540 2540 Size: 5761 FspI TGC'GCA Cuts at: 4267 4267 Size: 5761 HaeII r_GCGC'y Cuts at: 1678 4908 1678 Size: 3230 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2839 2871 4402 4434 4476 Size: 32 135 32 1267 32 1531 32 42 Cuts at: 4476 4508 1373 Size: 32 2626 Fragments arranged by size: 2626 1531 1267 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2309 3105 3351 669 Size: 988 281 371 796 246 3079 Fragments arranged by size: 3079 988 796 371 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5761 HpaI GTT'AAC Cuts at: 3351 3351 Size: 5761 KpnI G_GTAC'C Cuts at: 2386 3086 2386 Size: 700 5061 MfeI C'AATT_G Cuts at: 3360 3360 Size: 5761 MluI A'CGCG_T Cuts at: 5637 5637 Size: 5761 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2808 4875 5059 1305 Size: 1503 2067 184 2007 Fragments arranged by size: 2067 2007 1503 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2222 10 Size: 54 1425 343 390 3549 Fragments arranged by size: 3549 1425 390 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2462 2723 3719 4078 4237 518 Size: 648 1296 261 996 359 159 2042 Fragments arranged by size: 2042 1296 996 648 359 261 159 MspA1I CmG'CkG Cuts at: 1606 2623 3177 3854 5002 5247 1606 Size: 1017 554 677 1148 245 2120 Fragments arranged by size: 2120 1148 1017 677 554 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4270 Fragments arranged by size: 4270 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5761 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5761 NotI GC'GGCC_GC Cuts at: 3208 3208 Size: 5761 NspI r_CATG'y Cuts at: 1132 1229 2152 3119 4664 1132 Size: 97 923 967 1545 2229 Fragments arranged by size: 2229 1545 967 923 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5761 PciI A'CATG_T Cuts at: 4660 4660 Size: 5761 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5761 PfoI T'CCnGG_A Cuts at: 2784 2784 Size: 5761 PmeI GTTT'AAAC Cuts at: 3065 3065 Size: 5761 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3830 3862 5343 5375 3830 Size: 32 1481 32 4216 Fragments arranged by size: 4216 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5761 PsiI TTA'TAA Cuts at: 3331 3331 Size: 5761 PstI C_TGCA'G Cuts at: 830 2540 3113 830 Size: 1710 573 3478 Fragments arranged by size: 3478 1710 573 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5761 SalI G'TCGA_C Cuts at: 1936 3103 1936 Size: 1167 4594 SapI GCTCTTCn'nnn_ Cuts at: 2183 2183 Size: 5761 SbfI CC_TGCA'GG Cuts at: 3113 3113 Size: 5761 ScaI AGT'ACT Cuts at: 4009 5721 4009 Size: 1712 4049 SfcI C'TryA_G Cuts at: 826 1045 2536 3109 4244 4925 5116 826 Size: 219 1491 573 1135 681 191 1471 Fragments arranged by size: 1491 1471 1135 681 573 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5761 SmaI CCC'GGG Cuts at: 1872 3088 1872 Size: 1216 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3865 4766 5028 820 Size: 197 926 1922 901 262 1553 Fragments arranged by size: 1922 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3552 493 Size: 3059 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5761 SphI G_CATG'C Cuts at: 1132 3119 1132 Size: 1987 3774 SspI AAT'ATT Cuts at: 5 52 3685 5 Size: 47 3633 2081 Fragments arranged by size: 3633 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5761 StyI C'CwwG_G Cuts at: 513 1065 2004 3200 513 Size: 552 939 1196 3074 Fragments arranged by size: 3074 1196 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2452 3788 3805 3958 4143 4588 325 Size: 1255 872 1336 17 153 185 445 1498 Fragments arranged by size: 1498 1336 1255 872 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4007 5719 96 Size: 274 80 33 51 1443 2030 1712 138 Fragments arranged by size: 2030 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5721 5753 5721 Size: 32 5729 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5761 XbaI T'CTAG_A Cuts at: 3097 3097 Size: 5761 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2288 2792 2288 Size: 504 5257 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5761 XmaI C'CCGG_G Cuts at: 1870 3086 1870 Size: 1216 4545 XmnI GAAnn'nnTTC Cuts at: 2523 2579 2780 3890 2523 Size: 56 201 1110 4394 Fragments arranged by size: 4394 1110 201 56 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3569 276 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI