(Circular) (Six-base) MAPSORT of: pFN21AB9836.seq Check: 1742 from: 1 to: 5758 pFN21AB9836 5758 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2581 2581 Size: 5758 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3568 278 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3101 1937 Size: 1164 4594 Acc65I G'GTAC_C Cuts at: 3079 3079 Size: 5758 AclI AA'CG_TT Cuts at: 1563 3885 4258 1563 Size: 2322 373 3063 Fragments arranged by size: 3063 2322 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2447 3821 5205 5473 1632 Size: 815 1374 1384 268 1917 Fragments arranged by size: 1917 1384 1374 815 268 AfeI AGC'GCT Cuts at: 2064 2064 Size: 5758 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5561 AflIII A'CryG_T Cuts at: 1203 4657 5634 1203 Size: 3454 977 1327 Fragments arranged by size: 3454 1327 977 AgeI A'CCGG_T Cuts at: 4589 4589 Size: 5758 AhdI GACnn_n'nnGTC Cuts at: 4487 4487 Size: 5758 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5758 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5758 AlwNI CAG_nnn'CTG Cuts at: 2839 2963 3501 5073 2839 Size: 124 538 1572 3524 Fragments arranged by size: 3524 1572 538 124 ApaI G_GGCC'C Cuts at: 2434 2434 Size: 5758 ApaLI G'TGCA_C Cuts at: 3815 4971 3815 Size: 1156 4602 ApoI r'AATT_y Cuts at: 1493 2327 2543 3067 3293 3445 5554 1493 Size: 834 216 524 226 152 2109 1697 Fragments arranged by size: 2109 1697 834 524 226 216 152 AseI AT'TA_AT Cuts at: 160 1032 4312 160 Size: 872 3280 1606 Fragments arranged by size: 3280 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 AvaI C'yCGr_G Cuts at: 1870 1943 2509 3083 1870 Size: 73 566 574 4545 Fragments arranged by size: 4545 574 566 73 AvrII C'CTAG_G Cuts at: 2744 2744 Size: 5758 BamHI G'GATC_C Cuts at: 3088 3455 3088 Size: 367 5391 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2593 3079 4534 618 Size: 325 217 627 806 486 1455 1842 Fragments arranged by size: 1842 1455 806 627 486 325 217 BanII G_rGCy'C Cuts at: 1391 2374 2434 3077 1391 Size: 983 60 643 4072 Fragments arranged by size: 4072 983 643 60 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2031 928 Size: 971 132 4655 Fragments arranged by size: 4655 971 132 BbvCI CC'TCA_GC Cuts at: 3009 3009 Size: 5758 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3949 3983 1903 Size: 34 2012 34 3678 Fragments arranged by size: 3678 2012 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3650 4860 3650 Size: 1210 4548 BclI T'GATC_A Cuts at: 1548 1548 Size: 5758 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2637 4369 136 Size: 107 122 71 631 605 965 1732 1525 Fragments arranged by size: 1732 1525 965 631 605 122 107 71 BglII A'GATC_T Cuts at: 5753 5753 Size: 5758 BlpI GC'TnA_GC Cuts at: 3175 3175 Size: 5758 Bme1580I G_kGCm'C Cuts at: 1790 2434 3819 4975 1790 Size: 644 1385 1156 2573 Fragments arranged by size: 2573 1385 1156 644 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5758 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3489 4447 442 Size: 416 53 83 399 24 2072 958 1753 Fragments arranged by size: 2072 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5758 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2388 2388 Size: 5758 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2290 2508 2840 3014 4418 1239 Size: 1051 218 332 174 1404 2579 Fragments arranged by size: 2579 1404 1051 332 218 174 Bpu10I CC'TnA_GC Cuts at: 3009 3009 Size: 5758 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2651 2735 3883 4748 5046 2651 Size: 84 1148 865 298 3363 Fragments arranged by size: 3363 1148 865 298 84 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4421 882 Size: 617 2922 2219 Fragments arranged by size: 2922 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3549 493 Size: 711 2345 2702 Fragments arranged by size: 2702 2345 711 BsaBI GATnn'nnATC Cuts at: 5752 5752 Size: 5758 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3565 3947 4582 275 Size: 53 83 186 936 2032 382 635 1451 Fragments arranged by size: 2032 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4191 4589 4863 5010 5421 4191 Size: 398 274 147 411 4528 Fragments arranged by size: 4528 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2813 2843 1178 Size: 30 165 30 1410 30 4093 Fragments arranged by size: 4093 1410 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2126 2732 2865 3026 3029 3032 1186 Size: 940 606 133 161 3 3 3912 Fragments arranged by size: 3912 940 606 161 133 3 3 BseYI C'CCAG_C Cuts at: 2641 2903 2956 4961 5457 2641 Size: 262 53 2005 496 2942 Fragments arranged by size: 2942 2005 496 262 53 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2053 1163 Size: 573 317 4868 Fragments arranged by size: 4868 573 317 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3208 3969 4997 1540 Size: 275 186 1207 761 1028 2301 Fragments arranged by size: 2301 1207 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2374 3819 3904 4975 2374 Size: 1445 85 1071 3157 Fragments arranged by size: 3157 1445 1071 85 BsmI GAATG_Cn' Cuts at: 3269 3362 3269 Size: 93 5665 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2374 2434 3077 3819 3904 4975 1391 Size: 399 584 60 643 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 643 584 399 85 60 BspEI T'CCGG_A Cuts at: 5421 5421 Size: 5758 BspHI T'CATG_A Cuts at: 3647 5377 3647 Size: 1730 4028 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2581 2746 3113 844 Size: 1737 165 367 3489 Fragments arranged by size: 3489 1737 367 165 BsrBI CCG'CTC Cuts at: 1394 1823 3645 1394 Size: 429 1822 3507 Fragments arranged by size: 3507 1822 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2483 2898 4253 4427 65 Size: 1183 1235 415 1355 174 1396 Fragments arranged by size: 1396 1355 1235 1183 415 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2075 4402 4589 1700 Size: 110 265 2327 187 2869 Fragments arranged by size: 2869 2327 265 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5758 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5758 BssSI C'ACGA_G Cuts at: 3818 4830 3818 Size: 1012 4746 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5758 BstBI TT'CG_AA Cuts at: 4562 4562 Size: 5758 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5758 BstYI r'GATC_y Cuts at: 1972 3088 3455 3840 3857 5309 5395 5407 5753 Size: 1116 367 385 17 1452 86 12 346 Cuts at: 5753 1972 Size: 1977 Fragments arranged by size: 1977 1452 1116 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2241 2241 Size: 5758 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5206 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5019 Fragments arranged by size: 5019 683 56 BtsI GCAGTG_nn' Cuts at: 2101 3274 4068 4088 2101 Size: 1173 794 20 3771 Fragments arranged by size: 3771 1173 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5758 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5723 DraI TTT'AAA Cuts at: 3062 3062 Size: 5758 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4765 809 Size: 507 3449 1802 Fragments arranged by size: 3449 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3205 4094 5723 Size: 54 1425 50 275 18 1375 889 1629 Cuts at: 5723 8 Size: 43 Fragments arranged by size: 1629 1425 1375 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3205 1537 Size: 275 1393 4090 Fragments arranged by size: 4090 1393 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2466 2603 3688 1353 Size: 1113 137 1085 3423 Fragments arranged by size: 3423 1113 1085 137 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4345 4719 4865 387 Size: 1064 138 2756 374 146 1280 Fragments arranged by size: 2756 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2372 2372 Size: 5758 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2290 2447 2508 2840 3014 3821 4418 Size: 393 658 157 61 332 174 807 597 Cuts at: 4418 5205 5473 1239 Size: 787 268 1524 Fragments arranged by size: 1524 807 787 658 597 393 332 268 174 157 61 EcoNI CCTnn'n_nnAGG Cuts at: 3029 3029 Size: 5758 EcoO109I rG'GnC_Cy Cuts at: 2431 3022 2431 Size: 591 5167 EcoRI G'AATT_C Cuts at: 3067 3445 3067 Size: 378 5380 FspI TGC'GCA Cuts at: 4264 4264 Size: 5758 HaeII r_GCGC'y Cuts at: 1678 2066 4905 1678 Size: 388 2839 2531 Fragments arranged by size: 2839 2531 388 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2662 2694 4399 4431 4473 Size: 32 135 32 1090 32 1705 32 42 Cuts at: 4473 4505 1373 Size: 32 2626 Fragments arranged by size: 2626 1705 1090 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3102 3348 669 Size: 988 281 1164 246 3079 Fragments arranged by size: 3079 1164 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5758 HpaI GTT'AAC Cuts at: 3348 3348 Size: 5758 KpnI G_GTAC'C Cuts at: 3083 3083 Size: 5758 MfeI C'AATT_G Cuts at: 2856 3357 2856 Size: 501 5257 MluI A'CGCG_T Cuts at: 5634 5634 Size: 5758 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4872 5056 1305 Size: 3567 184 2007 Fragments arranged by size: 3567 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3936 Fragments arranged by size: 3936 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2351 2385 3716 4075 4234 518 Size: 648 1185 34 1331 359 159 2042 Fragments arranged by size: 2042 1331 1185 648 359 159 34 MspA1I CmG'CkG Cuts at: 1606 3008 3174 3851 4999 5244 1606 Size: 1402 166 677 1148 245 2120 Fragments arranged by size: 2120 1402 1148 677 245 166 NaeI GCC'GGC Cuts at: 2077 2077 Size: 5758 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5206 NdeI CA'TA_TG Cuts at: 387 387 Size: 5758 NgoMIV G'CCGG_C Cuts at: 2075 2075 Size: 5758 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5758 NotI GC'GGCC_GC Cuts at: 3205 3205 Size: 5758 NspI r_CATG'y Cuts at: 1132 1229 3116 4661 1132 Size: 97 1887 1545 2229 Fragments arranged by size: 2229 1887 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5758 PciI A'CATG_T Cuts at: 4657 4657 Size: 5758 PflMI CCAn_nnn'nTGG Cuts at: 1108 2951 2963 1108 Size: 1843 12 3903 Fragments arranged by size: 3903 1843 12 PfoI T'CCnGG_A Cuts at: 2418 2418 Size: 5758 PmeI GTTT'AAAC Cuts at: 3062 3062 Size: 5758 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3827 3859 5340 5372 3827 Size: 32 1481 32 4213 Fragments arranged by size: 4213 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5758 PsiI TTA'TAA Cuts at: 3328 3328 Size: 5758 PspOMI G'GGCC_C Cuts at: 2430 2430 Size: 5758 PstI C_TGCA'G Cuts at: 830 2096 2124 2709 3110 830 Size: 1266 28 585 401 3478 Fragments arranged by size: 3478 1266 585 401 28 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5758 PvuII CAG'CTG Cuts at: 3008 3008 Size: 5758 SacI G_AGCT'C Cuts at: 2374 2374 Size: 5758 SalI G'TCGA_C Cuts at: 1936 3100 1936 Size: 1164 4594 SapI GCTCTTCn'nnn_ Cuts at: 2466 2466 Size: 5758 SbfI CC_TGCA'GG Cuts at: 3110 3110 Size: 5758 ScaI AGT'ACT Cuts at: 4006 5718 4006 Size: 1712 4046 SfcI C'TryA_G Cuts at: 826 1045 2092 2120 2705 3106 4241 4922 5113 Size: 219 1047 28 585 401 1135 681 191 Cuts at: 5113 826 Size: 1471 Fragments arranged by size: 1471 1135 1047 681 585 401 219 191 28 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5758 SmaI CCC'GGG Cuts at: 1872 3085 1872 Size: 1213 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2666 2714 3862 4763 5025 820 Size: 197 926 723 48 1148 901 262 1553 Fragments arranged by size: 1553 1148 926 901 723 262 197 48 SnaBI TAC'GTA Cuts at: 493 3549 493 Size: 3056 2702 SpeI A'CTAG_T Cuts at: 152 2222 152 Size: 2070 3688 SphI G_CATG'C Cuts at: 1132 3116 1132 Size: 1984 3774 SspI AAT'ATT Cuts at: 5 52 3039 3682 5 Size: 47 2987 643 2081 Fragments arranged by size: 2987 2081 643 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5758 StyI C'CwwG_G Cuts at: 513 1065 2139 2744 3197 513 Size: 552 1074 605 453 3074 Fragments arranged by size: 3074 1074 605 552 453 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2345 3785 3802 3955 4140 4585 325 Size: 1255 765 1440 17 153 185 445 1498 Fragments arranged by size: 1498 1440 1255 765 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2148 2809 4004 Size: 274 80 33 51 1443 171 661 1195 Cuts at: 4004 5716 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1195 661 274 171 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5718 5750 5718 Size: 32 5726 Tth111I GACn'n_nGTC Cuts at: 1316 2894 1316 Size: 1578 4180 XbaI T'CTAG_A Cuts at: 3094 3094 Size: 5758 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2900 2900 Size: 5758 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5758 XmaI C'CCGG_G Cuts at: 1870 3083 1870 Size: 1213 4545 XmnI GAAnn'nnTTC Cuts at: 3887 3887 Size: 5758 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3566 276 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI