Restriction map for
pFN21AB9832
(Circular) (Six-base) MAPSORT of: pFN21AB9832.seq Check: 4236 from: 1 to: 5734
pFN21AB9832 5734 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3544 278
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3077 1937
Size: 1140 4594
Acc65I G'GTAC_C
Cuts at: 3055 3055
Size: 5734
AclI AA'CG_TT
Cuts at: 1563 3861 4234 1563
Size: 2298 373 3063
Fragments arranged by size:
3063 2298 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2180 2208 2345 3797 5181 5449 1632
Size: 548 28 137 1452 1384 268 1917
Fragments arranged by size:
1917 1452 1384 548 268 137 28
AfeI AGC'GCT
Cuts at: 2193 2193
Size: 5734
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5537
AflIII A'CryG_T
Cuts at: 1203 4633 5610 1203
Size: 3430 977 1327
Fragments arranged by size:
3430 1327 977
AgeI A'CCGG_T
Cuts at: 4565 4565
Size: 5734
AhdI GACnn_n'nnGTC
Cuts at: 4463 4463
Size: 5734
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5734
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2541 1184
Size: 1357 4377
AlwNI CAG_nnn'CTG
Cuts at: 2077 3477 5049 2077
Size: 1400 1572 2762
Fragments arranged by size:
2762 1572 1400
ApaLI G'TGCA_C
Cuts at: 3791 4947 3791
Size: 1156 4578
ApoI r'AATT_y
Cuts at: 1493 2155 2583 2807 3043 3269 3421 5530 1493
Size: 662 428 224 236 226 152 2109 1697
Fragments arranged by size:
2109 1697 662 428 236 226 224 152
AseI AT'TA_AT
Cuts at: 160 1032 4288 160
Size: 872 3256 1606
Fragments arranged by size:
3256 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 3059 1870
Size: 73 67 1049 4545
Fragments arranged by size:
4545 1049 73 67
BamHI G'GATC_C
Cuts at: 2506 2719 3064 3431 2506
Size: 213 345 367 4809
Fragments arranged by size:
4809 367 345 213
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2255 3055 4510 618
Size: 325 217 627 468 800 1455 1842
Fragments arranged by size:
1842 1455 800 627 468 325 217
BanII G_rGCy'C
Cuts at: 1391 2206 2830 2971 3053 1391
Size: 815 624 141 82 4072
Fragments arranged by size:
4072 815 624 141 82
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4763
BbvCI CC'TCA_GC
Cuts at: 2301 2301
Size: 5734
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3925 3959 1903
Size: 34 50 34 1904 34 3678
Fragments arranged by size:
3678 1904 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3626 4836 3626
Size: 1210 4524
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5734
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2099 4345 136
Size: 107 122 71 631 605 427 2246 1525
Fragments arranged by size:
2246 1525 631 605 427 122 107 71
BglII A'GATC_T
Cuts at: 5729 5729
Size: 5734
BlpI GC'TnA_GC
Cuts at: 3151 3151
Size: 5734
Bme1580I G_kGCm'C
Cuts at: 1790 2038 2647 2784 3795 4951 1790
Size: 248 609 137 1011 1156 2573
Fragments arranged by size:
2573 1156 1011 609 248 137
BmgBI CAC'GTC
Cuts at: 1318 3009 1318
Size: 1691 4043
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2624 3465 4423
Size: 416 53 83 399 24 1207 841 958
Cuts at: 4423 442
Size: 1753
Fragments arranged by size:
1753 1207 958 841 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5734
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2134 2134
Size: 5734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2786 2879 4394 1239
Size: 1547 93 1515 2579
Fragments arranged by size:
2579 1547 1515 93
Bpu10I CC'TnA_GC
Cuts at: 2301 3030 2301
Size: 729 5005
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2275 2435 3859 4724 5022 2275
Size: 160 1424 865 298 2987
Fragments arranged by size:
2987 1424 865 298 160
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4397 882
Size: 617 2898 2219
Fragments arranged by size:
2898 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3525 493
Size: 711 2321 2702
Fragments arranged by size:
2702 2321 711
BsaBI GATnn'nnATC
Cuts at: 5728 5728
Size: 5734
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3541 3923 4558 275
Size: 53 83 186 936 2008 382 635 1451
Fragments arranged by size:
2008 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4167 4565 4839 4986 5397 4167
Size: 398 274 147 411 4504
Fragments arranged by size:
4504 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5509
Fragments arranged by size:
5509 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5734
BseYI C'CCAG_C
Cuts at: 4937 5433 4937
Size: 496 5238
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2771 1163
Size: 573 1035 4126
Fragments arranged by size:
4126 1035 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3184 3945 4973 1540
Size: 275 186 1183 761 1028 2301
Fragments arranged by size:
2301 1183 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2469 2830 3795 3880 4951 2469
Size: 361 965 85 1071 3252
Fragments arranged by size:
3252 1071 965 361 85
BsmI GAATG_Cn'
Cuts at: 3245 3338 3245
Size: 93 5641
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 2206 2469 2647 2784 2830 2971
Size: 399 248 168 263 178 137 46 141
Cuts at: 2971 3053 3795 3880 4951 1391
Size: 82 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 263 248 178 168
141 137 85 82 46
BspEI T'CCGG_A
Cuts at: 5397 5397
Size: 5734
BspHI T'CATG_A
Cuts at: 2733 3623 5353 2733
Size: 890 1730 3114
Fragments arranged by size:
3114 1730 890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3089 844
Size: 2245 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3621 1394
Size: 429 1798 3507
Fragments arranged by size:
3507 1798 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4229 4403 65
Size: 1183 2981 174 1396
Fragments arranged by size:
2981 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4378 4565 1700
Size: 110 2568 187 2869
Fragments arranged by size:
2869 2568 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5734
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5734
BssSI C'ACGA_G
Cuts at: 3794 4806 3794
Size: 1012 4722
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2356 794
Size: 1562 4172
BstBI TT'CG_AA
Cuts at: 4538 4538
Size: 5734
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5734
BstXI CCAn_nnnn'nTGG
Cuts at: 2746 2746
Size: 5734
BstYI r'GATC_y
Cuts at: 1972 2074 2317 2445 2506 2719 3064 3431 3816
Size: 102 243 128 61 213 345 367 385
Cuts at: 3816 3833 5285 5371 5383 5729 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 345 243 213
128 102 86 61 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2982 513
Size: 552 1917 3265
Fragments arranged by size:
3265 1917 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4995
Fragments arranged by size:
4995 683 56
BtsI GCAGTG_nn'
Cuts at: 3250 4044 4064 3250
Size: 794 20 4920
Fragments arranged by size:
4920 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5734
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5699
DraI TTT'AAA
Cuts at: 2325 3038 2325
Size: 713 5021
DraIII CAC_nnn'GTG
Cuts at: 2486 2618 2486
Size: 132 5602
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4741 809
Size: 507 3425 1802
Fragments arranged by size:
3425 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3181 4070 5699
Size: 54 1425 50 275 18 1351 889 1629
Cuts at: 5699 8
Size: 43
Fragments arranged by size:
1629 1425 1351 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3181 1537
Size: 275 1369 4090
Fragments arranged by size:
4090 1369 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2015 2646 2910 3664 1353
Size: 662 631 264 754 3423
Fragments arranged by size:
3423 754 662 631 264
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4321 4695 4841 387
Size: 1064 138 2732 374 146 1280
Fragments arranged by size:
2732 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2828 2828
Size: 5734
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 2208 2345 2786 2879 3797 4394
Size: 393 548 28 137 441 93 918 597
Cuts at: 4394 5181 5449 1239
Size: 787 268 1524
Fragments arranged by size:
1524 918 787 597 548 441 393 268
137 93 28
EcoRI G'AATT_C
Cuts at: 2807 3043 3421 2807
Size: 236 378 5120
Fragments arranged by size:
5120 378 236
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2452 2452
Size: 5734
FspI TGC'GCA
Cuts at: 4240 4240
Size: 5734
HaeII r_GCGC'y
Cuts at: 1678 2195 4881 1678
Size: 517 2686 2531
Fragments arranged by size:
2686 2531 517
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4375 4407 4449 4481 1373
Size: 32 135 32 2803 32 42 32 2626
Fragments arranged by size:
2803 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3078 3324 669
Size: 988 281 1140 246 3079
Fragments arranged by size:
3079 1140 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5734
HpaI GTT'AAC
Cuts at: 3324 3324
Size: 5734
KpnI G_GTAC'C
Cuts at: 3059 3059
Size: 5734
MfeI C'AATT_G
Cuts at: 3333 3333
Size: 5734
MluI A'CGCG_T
Cuts at: 5610 5610
Size: 5734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4848 5032 1305
Size: 3543 184 2007
Fragments arranged by size:
3543 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3912
Fragments arranged by size:
3912 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2439 2523 3692 4051 4210 518
Size: 648 1273 84 1169 359 159 2042
Fragments arranged by size:
2042 1273 1169 648 359 159 84
MspA1I CmG'CkG
Cuts at: 1606 3150 3827 4975 5220 1606
Size: 1544 677 1148 245 2120
Fragments arranged by size:
2120 1544 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2982 513
Size: 552 1917 3265
Fragments arranged by size:
3265 1917 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5734
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5734
NotI GC'GGCC_GC
Cuts at: 3181 3181
Size: 5734
NspI r_CATG'y
Cuts at: 1132 1229 3092 4637 1132
Size: 97 1863 1545 2229
Fragments arranged by size:
2229 1863 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5734
PciI A'CATG_T
Cuts at: 4633 4633
Size: 5734
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5734
PfoI T'CCnGG_A
Cuts at: 2856 2856
Size: 5734
PmeI GTTT'AAAC
Cuts at: 3038 3038
Size: 5734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3803 3835 5316 5348 3803
Size: 32 1481 32 4189
Fragments arranged by size:
4189 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5734
PsiI TTA'TAA
Cuts at: 3304 3304
Size: 5734
PspXI vC'TCGA_Gb
Cuts at: 2010 2010
Size: 5734
PstI C_TGCA'G
Cuts at: 830 2962 3086 830
Size: 2132 124 3478
Fragments arranged by size:
3478 2132 124
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5734
SacI G_AGCT'C
Cuts at: 2830 2830
Size: 5734
SalI G'TCGA_C
Cuts at: 1936 3076 1936
Size: 1140 4594
SbfI CC_TGCA'GG
Cuts at: 3086 3086
Size: 5734
ScaI AGT'ACT
Cuts at: 3982 5694 3982
Size: 1712 4022
SfcI C'TryA_G
Cuts at: 826 1045 2958 3082 4217 4898 5089 826
Size: 219 1913 124 1135 681 191 1471
Fragments arranged by size:
1913 1471 1135 681 219 191 124
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5734
SmaI CCC'GGG
Cuts at: 1872 3061 1872
Size: 1189 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2010 2290 2450 3838 4739 5001
Size: 197 926 67 280 160 1388 901 262
Cuts at: 5001 820
Size: 1553
Fragments arranged by size:
1553 1388 926 901 280 262 197 160
67
SnaBI TAC'GTA
Cuts at: 493 3525 493
Size: 3032 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5734
SphI G_CATG'C
Cuts at: 1132 3092 1132
Size: 1960 3774
SspI AAT'ATT
Cuts at: 5 52 3658 5
Size: 47 3606 2081
Fragments arranged by size:
3606 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5734
StyI C'CwwG_G
Cuts at: 513 1065 2982 3173 513
Size: 552 1917 191 3074
Fragments arranged by size:
3074 1917 552 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2606 2672 3761 3778 3931 4116 4561
Size: 1255 1026 66 1089 17 153 185 445
Cuts at: 4561 325
Size: 1498
Fragments arranged by size:
1498 1255 1089 1026 445 185 153 66
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3980 5692 96
Size: 274 80 33 51 1443 2003 1712 138
Fragments arranged by size:
2003 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2166 2198 5694 5726 2166
Size: 32 3496 32 2174
Fragments arranged by size:
3496 2174 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5734
XbaI T'CTAG_A
Cuts at: 3070 3070
Size: 5734
XhoI C'TCGA_G
Cuts at: 1943 2010 1943
Size: 67 5667
XmaI C'CCGG_G
Cuts at: 1870 3059 1870
Size: 1189 4545
XmnI GAAnn'nnTTC
Cuts at: 2193 2583 2705 3863 2193
Size: 390 122 1158 4064
Fragments arranged by size:
4064 1158 390 122
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3542 276
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspXI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI