Restriction map for
pFN21AB9830
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccII AciI AclI
AcyI AflIII AhdI AluI AlwNI
AocI Aor51HI ApaI AscI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaHI BsaI BseRI BsiWI BsmBI
Bsp1407I BspLU11I BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I Bsu36I CelII
CpoI DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRV EcoT14I FauI FbaI
FseI FspI HaeII HgaI Hin1I
HindIII KasI KpnI KspI MfeI
MluI MluNI MscI MslI MspA1I
MunI MvnI MwoI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuII RsrII SacI
SacII SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SpeI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 74 enzymes) #
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AccI 10 GTCGAC GT^[AC][GT]AC
AccIII 158 TCCGGA T^CCGGA
AfaI 648 GTAC GT^AC
AflII 187 CTTAAG C^TTAAG
AgeI 650 ACCGGT A^CCGGT
Alw44I 48 GTGCAC G^TGCAC
ApaLI 48 GTGCAC G^TGCAC
AseI 598 ATTAAT AT^TAAT
AsnI 598 ATTAAT AT^TAAT
AvaI 791 CCCGGG C^[CT]CG[GA]G
BamHI 879 GGATCC G^GATCC
BbsI 372 GAAGAC GAAGAC(2/6)
BbvI 495 GCAGC GCAGC(8/12)
BfaI 550 CTAG C^TAG
BfrI 187 CTTAAG C^TTAAG
BpuAI 372 GAAGAC GAAGAC(2/6)
BsaAI 658 TACGTA [CT]AC^GT[GA]
BsaBI 351 GATAACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 158 TCCGGA T^CCGGA
BsgI 807 CTGCAC GTGCAG(16/14)
BsiEI 360 CGATCG CG[GA][CT]^CG
BsiYI 786 CCAAACCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 786 CCAAACCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 448 GCATTC GAATGC(1/-1)
BsoBI 791 CCCGGG C^[CT]CG[GA]G
BspDI 358 ATCGAT AT^CGAT
BspEI 158 TCCGGA T^CCGGA
BspHI 215 TCATGA T^CATGA
BsrDI 723 CATTGC GCAATG(2/0)
BsrFI 650 ACCGGT [GA]^CCGG[CT]
BstEII 1000 GGTGACC G^GT[ACGT]ACC
BstPI 1000 GGTGACC G^GT[ACGT]ACC
BstYI 879 GGATCC [GA]^GATC[CT]
CfoI 28 GCGC GCG^C
Cfr10I 650 ACCGGT [GA]^CCGG[CT]
ClaI 358 ATCGAT AT^CGAT
DraI 388 TTTAAA TTT^AAA
DraII 74 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 846 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 74 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1000 GGTGACC G^GT[ACGT]ACC
EcoRI 767 GAATTC G^AATTC
EcoT22I 869 ATGCAT ATGCA^T
Fnu4HI 495 GCAGC GC^[ACGT]GC
HhaI 28 GCGC GCG^C
HinP1I 28 GCGC G^CGC
HpaI 855 GTTAAC GTT^AAC
ItaI 495 GCAGC GC^[ACGT]GC
MaeI 550 CTAG C^TAG
MamI 351 GATAACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 879 GGATCC [GA]^GATC[CT]
MroI 158 TCCGGA T^CCGGA
NlaIV 879 GGATCC GG[ACGT]^[ACGT]CC
NsiI 869 ATGCAT ATGCA^T
NspI 871 GCATGC [GA]CATG^[CT]
PinAI 650 ACCGGT A^CCGGT
PleI 1017 GACTC GAGTC(4/5)
PpuMI 74 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 598 ATTAAT AT^TAAT
PvuI 360 CGATCG CGAT^CG
RcaI 215 TCATGA T^CATGA
RsaI 648 GTAC GT^AC
SalI 10 GTCGAC G^TCGAC
SfaNI 250 GATGC GCATC(5/9)
SmaI 791 CCCGGG CCC^GGG
SmlI 187 CTTAAG C^T[CT][GA]AG
SnaBI 658 TACGTA TAC^GTA
SphI 871 GCATGC GCATG^C
TseI 495 GCAGC G^C[AT]GC
Tsp45I 1001 GTGAC ^GT[GC]AC
XcmI 404 CCAAAAATCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 879 GGATCC [GA]^GATC[CT]
XmaCI 791 CCCGGG C^CCGGG
XmaI 791 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 880 GATCC GGATC(4/5)
879 GGATC
AspHI 48 GTGCAC G[AT]GC[AT]^C
843 GAGCAC
AvaII 75 GGTCC G^G[AT]CC
268 GGACC
BcnI 791 CCCGG CC^[GC]GG
792 CCGGG
BsaWI 158 TCCGGA [AT]^CCGG[AT]
650 ACCGGT
BsiHKAI 48 GTGCAC G[AT]GC[AT]^C
843 GAGCAC
BsrI 258 ACTGG ACTGG(1/-1)
41 CCAGT
Cac8I 3 GCAAGC GC[ACGT]^[ACGT]GC
871 GCATGC
Cfr13I 75 GGTCC G^G[ACGT]CC
268 GGACC
EarI 79 CTCTTC CTCTTC(1/4)
1012 GAAGAG
HaeIII 130 GGCC GG^CC
383 GGCC
HincII 10 GTCGAC GT[CT]^[GA]AC
855 GTTAAC
HindII 10 GTCGAC GT[CT]^[GA]AC
855 GTTAAC
Ksp632I 79 CTCTTC CTCTTC(1/4)
1012 GAAGAG
MaeII 659 ACGT A^CGT
704 ACGT
MaeIII 295 GTTAC ^GT[ACGT]AC
1001 GTGAC
NciI 791 CCCGG CC^[GC]GG
792 CCGGG
Sau96I 75 GGTCC G^G[ACGT]CC
268 GGACC
TaqI 11 TCGA T^CGA
359 TCGA
TthHB8I 11 TCGA T^CGA
359 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AcsI ApoI BmyI BsaJI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
DdeI DpnII EcoRII FokI HapII
HinfI HpaII HphI MboI MboII
MnlI MseI MspI MvaI NdeII
NlaIII Sau3AI ScrFI TfiI Tru9I
Tsp509I TspRI