(Circular) (Six-base) MAPSORT of: pFN21AB9830.seq Check: 9704 from: 1 to: 5731 pFN21AB9830 5731 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3541 278 Size: 53 83 186 936 2005 2468 Fragments arranged by size: 2468 2005 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2016 3074 1937 Size: 79 1058 4594 Fragments arranged by size: 4594 1058 79 Acc65I G'GTAC_C Cuts at: 3052 3052 Size: 5731 AclI AA'CG_TT Cuts at: 1563 3858 4231 1563 Size: 2295 373 3063 Fragments arranged by size: 3063 2295 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2902 2963 3794 5178 5446 1632 Size: 1270 61 831 1384 268 1917 Fragments arranged by size: 1917 1384 1270 831 268 61 AflII C'TTAA_G Cuts at: 820 1017 2192 820 Size: 197 1175 4359 Fragments arranged by size: 4359 1175 197 AflIII A'CryG_T Cuts at: 1203 4630 5607 1203 Size: 3427 977 1327 Fragments arranged by size: 3427 1327 977 AgeI A'CCGG_T Cuts at: 2655 4562 2655 Size: 1907 3824 AhdI GACnn_n'nnGTC Cuts at: 4460 4460 Size: 5731 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5731 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5731 AlwNI CAG_nnn'CTG Cuts at: 3474 5046 3474 Size: 1572 4159 ApaLI G'TGCA_C Cuts at: 2053 3788 4944 2053 Size: 1735 1156 2840 Fragments arranged by size: 2840 1735 1156 ApoI r'AATT_y Cuts at: 1493 2244 2772 2923 3040 3266 3418 5527 1493 Size: 751 528 151 117 226 152 2109 1697 Fragments arranged by size: 2109 1697 751 528 226 152 151 117 AseI AT'TA_AT Cuts at: 160 1032 2604 4285 160 Size: 872 1572 1681 1606 Fragments arranged by size: 1681 1606 1572 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5731 AvaI C'yCGr_G Cuts at: 1870 1943 2796 3056 1870 Size: 73 853 260 4545 Fragments arranged by size: 4545 853 260 73 BamHI G'GATC_C Cuts at: 2884 3061 3428 2884 Size: 177 367 5187 Fragments arranged by size: 5187 367 177 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3052 4507 618 Size: 325 217 627 1265 1455 1842 Fragments arranged by size: 1842 1455 1265 627 325 217 BanII G_rGCy'C Cuts at: 1391 3050 1391 Size: 1659 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2384 928 Size: 971 485 4275 Fragments arranged by size: 4275 971 485 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3922 3956 1903 Size: 34 50 34 1901 34 3678 Fragments arranged by size: 3678 1901 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3623 4833 3623 Size: 1210 4521 BclI T'GATC_A Cuts at: 1548 1548 Size: 5731 BfrBI ATG'CAT Cuts at: 2876 2876 Size: 5731 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4342 136 Size: 107 122 71 631 605 2670 1525 Fragments arranged by size: 2670 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5726 5726 Size: 5731 BlpI GC'TnA_GC Cuts at: 3148 3148 Size: 5731 Bme1580I G_kGCm'C Cuts at: 1790 2057 2951 3792 4948 1790 Size: 267 894 841 1156 2573 Fragments arranged by size: 2573 1156 894 841 267 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5731 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2273 3462 4420 Size: 416 53 83 399 24 856 1189 958 Cuts at: 4420 442 Size: 1753 Fragments arranged by size: 1753 1189 958 856 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5731 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4391 1239 Size: 3152 2579 Bpu10I CC'TnA_GC Cuts at: 2786 2786 Size: 5731 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3856 4721 5019 3856 Size: 865 298 4568 Fragments arranged by size: 4568 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4394 882 Size: 617 2895 2219 Fragments arranged by size: 2895 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2665 3522 493 Size: 711 1461 857 2702 Fragments arranged by size: 2702 1461 857 711 BsaBI GATnn'nnATC Cuts at: 2360 5725 2360 Size: 3365 2366 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3538 3920 4555 275 Size: 53 83 186 936 2005 382 635 1451 Fragments arranged by size: 2005 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2163 2655 4164 4562 4836 4983 5394 2163 Size: 492 1509 398 274 147 411 2500 Fragments arranged by size: 2500 1509 492 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5506 Fragments arranged by size: 5506 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5731 BseYI C'CCAG_C Cuts at: 4934 5430 4934 Size: 496 5235 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2797 1163 Size: 573 1061 4097 Fragments arranged by size: 4097 1061 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2368 3181 3942 4970 1540 Size: 275 186 367 813 761 1028 2301 Fragments arranged by size: 2301 1028 813 761 367 275 186 BsiHKAI G_wGCw'C Cuts at: 2057 2852 3792 3877 4948 2057 Size: 795 940 85 1071 2840 Fragments arranged by size: 2840 1071 940 795 85 BsmI GAATG_Cn' Cuts at: 2453 3242 3335 2453 Size: 789 93 4849 Fragments arranged by size: 4849 789 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2852 2951 3050 3792 3877 4948 Size: 399 267 795 99 99 742 85 1071 Cuts at: 4948 1391 Size: 2174 Fragments arranged by size: 2174 1071 795 742 399 267 99 99 85 BspEI T'CCGG_A Cuts at: 2163 5394 2163 Size: 3231 2500 BspHI T'CATG_A Cuts at: 2220 3620 5350 2220 Size: 1400 1730 2601 Fragments arranged by size: 2601 1730 1400 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3086 844 Size: 2242 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3618 1394 Size: 429 1795 3507 Fragments arranged by size: 3507 1795 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2727 4226 4400 65 Size: 1183 1479 1499 174 1396 Fragments arranged by size: 1499 1479 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2655 4375 4562 1700 Size: 110 845 1720 187 2869 Fragments arranged by size: 2869 1720 845 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5731 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5731 BssSI C'ACGA_G Cuts at: 3791 4803 3791 Size: 1012 4719 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5731 BstBI TT'CG_AA Cuts at: 4535 4535 Size: 5731 BstEII G'GTnAC_C Cuts at: 1184 3005 1184 Size: 1821 3910 BstYI r'GATC_y Cuts at: 1972 2884 3061 3428 3813 3830 5282 5368 5380 Size: 912 177 367 385 17 1452 86 12 Cuts at: 5380 5726 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 912 385 367 346 177 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5179 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2156 487 Size: 683 56 930 4062 Fragments arranged by size: 4062 930 683 56 BtsI GCAGTG_nn' Cuts at: 2090 2814 3247 4041 4061 2090 Size: 724 433 794 20 3760 Fragments arranged by size: 3760 794 724 433 20 ClaI AT'CG_AT Cuts at: 1554 2364 1554 Size: 810 4921 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5696 DraI TTT'AAA Cuts at: 2395 3035 2395 Size: 640 5091 DraIII CAC_nnn'GTG Cuts at: 2856 2856 Size: 5731 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4738 809 Size: 507 3422 1802 Fragments arranged by size: 3422 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3178 4067 5696 Size: 54 1425 50 275 18 1348 889 1629 Cuts at: 5696 8 Size: 43 Fragments arranged by size: 1629 1425 1348 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3178 1537 Size: 275 1366 4090 Fragments arranged by size: 4090 1366 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2090 3012 3661 1353 Size: 737 922 649 3423 Fragments arranged by size: 3423 922 737 649 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4318 4692 4838 387 Size: 1064 138 2729 374 146 1280 Fragments arranged by size: 2729 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2902 2963 3794 4391 5178 5446 1239 Size: 393 1270 61 831 597 787 268 1524 Fragments arranged by size: 1524 1270 831 787 597 393 268 61 EcoO109I rG'GnC_Cy Cuts at: 2080 2080 Size: 5731 EcoRI G'AATT_C Cuts at: 2772 3040 3418 2772 Size: 268 378 5085 Fragments arranged by size: 5085 378 268 FspI TGC'GCA Cuts at: 4237 4237 Size: 5731 HaeII r_GCGC'y Cuts at: 1678 4878 1678 Size: 3200 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4372 4404 4446 4478 1373 Size: 32 135 32 2800 32 42 32 2626 Fragments arranged by size: 2800 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2017 2862 3075 3321 669 Size: 988 281 79 845 213 246 3079 Fragments arranged by size: 3079 988 845 281 246 213 79 HindIII A'AGCT_T Cuts at: 748 748 Size: 5731 HpaI GTT'AAC Cuts at: 2862 3321 2862 Size: 459 5272 KpnI G_GTAC'C Cuts at: 3056 3056 Size: 5731 MfeI C'AATT_G Cuts at: 3330 3330 Size: 5731 MluI A'CGCG_T Cuts at: 5607 5607 Size: 5731 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2098 2427 4845 5029 1305 Size: 793 329 2418 184 2007 Fragments arranged by size: 2418 2007 793 329 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3909 Fragments arranged by size: 3909 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3689 4048 4207 518 Size: 648 2523 359 159 2042 Fragments arranged by size: 2523 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3147 3824 4972 5217 1606 Size: 1541 677 1148 245 2120 Fragments arranged by size: 2120 1541 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5179 NdeI CA'TA_TG Cuts at: 387 387 Size: 5731 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5731 NotI GC'GGCC_GC Cuts at: 3178 3178 Size: 5731 NsiI A_TGCA'T Cuts at: 2878 2878 Size: 5731 NspI r_CATG'y Cuts at: 1132 1229 2880 3089 4634 1132 Size: 97 1651 209 1545 2229 Fragments arranged by size: 2229 1651 1545 209 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5731 PciI A'CATG_T Cuts at: 4630 4630 Size: 5731 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5731 PmeI GTTT'AAAC Cuts at: 3035 3035 Size: 5731 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3800 3832 5313 5345 3800 Size: 32 1481 32 4186 Fragments arranged by size: 4186 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2080 2080 Size: 5731 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5731 PsiI TTA'TAA Cuts at: 3301 3301 Size: 5731 PstI C_TGCA'G Cuts at: 830 3083 830 Size: 2253 3478 PvuI CG_AT'CG Cuts at: 2001 2368 2001 Size: 367 5364 SalI G'TCGA_C Cuts at: 1936 2015 3073 1936 Size: 79 1058 4594 Fragments arranged by size: 4594 1058 79 SbfI CC_TGCA'GG Cuts at: 3083 3083 Size: 5731 ScaI AGT'ACT Cuts at: 3979 5691 3979 Size: 1712 4019 SfcI C'TryA_G Cuts at: 826 1045 3079 4214 4895 5086 826 Size: 219 2034 1135 681 191 1471 Fragments arranged by size: 2034 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5731 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5731 SmaI CCC'GGG Cuts at: 1872 2798 3058 1872 Size: 926 260 4545 Fragments arranged by size: 4545 926 260 SmlI C'TyrA_G Cuts at: 820 1017 1943 2192 3835 4736 4998 820 Size: 197 926 249 1643 901 262 1553 Fragments arranged by size: 1643 1553 926 901 262 249 197 SnaBI TAC'GTA Cuts at: 493 2665 3522 493 Size: 2172 857 2702 Fragments arranged by size: 2702 2172 857 SpeI A'CTAG_T Cuts at: 152 152 Size: 5731 SphI G_CATG'C Cuts at: 1132 2880 3089 1132 Size: 1748 209 3774 Fragments arranged by size: 3774 1748 209 SspI AAT'ATT Cuts at: 5 52 3655 5 Size: 47 3603 2081 Fragments arranged by size: 3603 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5731 StyI C'CwwG_G Cuts at: 513 1065 3170 513 Size: 552 2105 3074 Fragments arranged by size: 3074 2105 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2090 2540 3758 3775 3928 4113 4558 Size: 1255 510 450 1218 17 153 185 445 Cuts at: 4558 325 Size: 1498 Fragments arranged by size: 1498 1255 1218 510 450 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3977 5689 96 Size: 274 80 33 51 1443 2000 1712 138 Fragments arranged by size: 2000 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5691 5723 5691 Size: 32 5699 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5731 XbaI T'CTAG_A Cuts at: 3067 3067 Size: 5731 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2416 2416 Size: 5731 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5731 XmaI C'CCGG_G Cuts at: 1870 2796 3056 1870 Size: 926 260 4545 Fragments arranged by size: 4545 926 260 XmnI GAAnn'nnTTC Cuts at: 3860 3860 Size: 5731 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3539 276 Size: 53 83 186 936 2005 2468 Fragments arranged by size: 2468 2005 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI