(Circular) (Six-base) MAPSORT of: pFN21AB9828.seq Check: 7403 from: 1 to: 5695 pFN21AB9828 5695 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2860 2860 Size: 5695 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3505 278 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3038 1937 Size: 1101 4594 Acc65I G'GTAC_C Cuts at: 2229 3016 2229 Size: 787 4908 AclI AA'CG_TT Cuts at: 1563 3822 4195 1563 Size: 2259 373 3063 Fragments arranged by size: 3063 2259 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2820 3758 5142 5410 1632 Size: 1188 938 1384 268 1917 Fragments arranged by size: 1917 1384 1188 938 268 AfeI AGC'GCT Cuts at: 2324 2324 Size: 5695 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5498 AflIII A'CryG_T Cuts at: 1203 2721 4594 5571 1203 Size: 1518 1873 977 1327 Fragments arranged by size: 1873 1518 1327 977 AgeI A'CCGG_T Cuts at: 4526 4526 Size: 5695 AhdI GACnn_n'nnGTC Cuts at: 4424 4424 Size: 5695 AleI CACnn'nnGTG Cuts at: 1166 2432 1166 Size: 1266 4429 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5695 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2959 2991 2959 Size: 32 5663 AlwNI CAG_nnn'CTG Cuts at: 2875 3438 5010 2875 Size: 563 1572 3560 Fragments arranged by size: 3560 1572 563 ApaI G_GGCC'C Cuts at: 2647 2647 Size: 5695 ApaLI G'TGCA_C Cuts at: 2135 3752 4908 2135 Size: 1617 1156 2922 Fragments arranged by size: 2922 1617 1156 ApoI r'AATT_y Cuts at: 1493 3004 3230 3382 5491 1493 Size: 1511 226 152 2109 1697 Fragments arranged by size: 2109 1697 1511 226 152 AseI AT'TA_AT Cuts at: 160 1032 4249 160 Size: 872 3217 1606 Fragments arranged by size: 3217 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 AvaI C'yCGr_G Cuts at: 1870 1943 3020 1870 Size: 73 1077 4545 Fragments arranged by size: 4545 1077 73 BamHI G'GATC_C Cuts at: 2109 3025 3392 2109 Size: 916 367 4412 Fragments arranged by size: 4412 916 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2229 2655 3016 4471 618 Size: 325 217 627 442 426 361 1455 1842 Fragments arranged by size: 1842 1455 627 442 426 361 325 217 BanII G_rGCy'C Cuts at: 1391 2027 2647 2891 3014 1391 Size: 636 620 244 123 4072 Fragments arranged by size: 4072 636 620 244 123 BbeI G_GCGC'C Cuts at: 2659 2659 Size: 5695 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4724 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2299 2333 3886 3920 1903 Size: 34 50 34 278 34 1553 34 3678 Fragments arranged by size: 3678 1553 278 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3587 4797 3587 Size: 1210 4485 BclI T'GATC_A Cuts at: 1548 2436 1548 Size: 888 4807 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2093 2542 4306 Size: 107 122 71 631 605 421 449 1764 Cuts at: 4306 136 Size: 1525 Fragments arranged by size: 1764 1525 631 605 449 421 122 107 71 BglII A'GATC_T Cuts at: 5690 5690 Size: 5695 BlpI GC'TnA_GC Cuts at: 2150 3112 2150 Size: 962 4733 Bme1580I G_kGCm'C Cuts at: 1790 2139 2647 3756 4912 1790 Size: 349 508 1109 1156 2573 Fragments arranged by size: 2573 1156 1109 508 349 BmgBI CAC'GTC Cuts at: 1318 2527 1318 Size: 1209 4486 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3426 4384 442 Size: 416 53 83 399 24 2009 958 1753 Fragments arranged by size: 2009 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5695 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2567 4355 1239 Size: 1328 1788 2579 Fragments arranged by size: 2579 1788 1328 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3820 4685 4983 3820 Size: 865 298 4532 Fragments arranged by size: 4532 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4358 882 Size: 617 2859 2219 Fragments arranged by size: 2859 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2295 3486 493 Size: 711 1091 1191 2702 Fragments arranged by size: 2702 1191 1091 711 BsaBI GATnn'nnATC Cuts at: 5689 5689 Size: 5695 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2656 3502 3884 4519 Size: 53 83 186 936 1123 846 382 635 Cuts at: 4519 275 Size: 1451 Fragments arranged by size: 1451 1123 936 846 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4128 4526 4800 4947 5358 4128 Size: 398 274 147 411 4465 Fragments arranged by size: 4465 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2345 2375 1178 Size: 30 165 30 942 30 4498 Fragments arranged by size: 4498 942 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2162 2881 1186 Size: 976 719 4000 Fragments arranged by size: 4000 976 719 BseYI C'CCAG_C Cuts at: 2121 2628 4898 5394 2121 Size: 507 2270 496 2422 Fragments arranged by size: 2422 2270 507 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2112 2369 2410 1163 Size: 573 376 257 41 4448 Fragments arranged by size: 4448 573 376 257 41 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3145 3906 4934 1540 Size: 275 186 1144 761 1028 2301 Fragments arranged by size: 2301 1144 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2027 2139 2565 3756 3841 4912 2027 Size: 112 426 1191 85 1071 2810 Fragments arranged by size: 2810 1191 1071 426 112 85 BsmI GAATG_Cn' Cuts at: 3206 3299 3206 Size: 93 5602 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2027 2139 2565 2647 2891 3014 3756 Size: 399 237 112 426 82 244 123 742 Cuts at: 3756 3841 4912 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 426 399 244 237 123 112 85 82 BspEI T'CCGG_A Cuts at: 5358 5358 Size: 5695 BspHI T'CATG_A Cuts at: 3584 5314 3584 Size: 1730 3965 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2241 2458 2501 2860 3050 844 Size: 1397 217 43 359 190 3489 Fragments arranged by size: 3489 1397 359 217 190 43 BsrBI CCG'CTC Cuts at: 1394 1823 3582 1394 Size: 429 1759 3507 Fragments arranged by size: 3507 1759 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2488 4190 4364 65 Size: 1183 1240 1702 174 1396 Fragments arranged by size: 1702 1396 1240 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2367 4339 4526 1700 Size: 110 557 1972 187 2869 Fragments arranged by size: 2869 1972 557 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5695 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5695 BssSI C'ACGA_G Cuts at: 2257 3755 4767 2257 Size: 1498 1012 3185 Fragments arranged by size: 3185 1498 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2286 2875 794 Size: 1492 589 3614 Fragments arranged by size: 3614 1492 589 BstBI TT'CG_AA Cuts at: 4499 4499 Size: 5695 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5695 BstXI CCAn_nnnn'nTGG Cuts at: 2083 2083 Size: 5695 BstYI r'GATC_y Cuts at: 1972 2109 3025 3392 3777 3794 5246 5332 5344 Size: 137 916 367 385 17 1452 86 12 Cuts at: 5344 5690 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 916 385 367 346 137 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2577 2664 2778 513 Size: 552 1512 87 114 3430 Fragments arranged by size: 3430 1512 552 114 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4956 Fragments arranged by size: 4956 683 56 BtsI GCAGTG_nn' Cuts at: 2422 3211 4005 4025 2422 Size: 789 794 20 4092 Fragments arranged by size: 4092 794 789 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5695 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2760 2795 529 Size: 35 2196 35 3429 Fragments arranged by size: 3429 2196 35 35 DraI TTT'AAA Cuts at: 2999 2999 Size: 5695 DraIII CAC_nnn'GTG Cuts at: 2734 2734 Size: 5695 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4702 809 Size: 507 3386 1802 Fragments arranged by size: 3386 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2163 3142 4031 Size: 54 1425 50 275 18 333 979 889 Cuts at: 4031 5660 8 Size: 1629 43 Fragments arranged by size: 1629 1425 979 889 333 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3142 1537 Size: 275 1330 4090 Fragments arranged by size: 4090 1330 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2015 2570 2825 2836 3625 1353 Size: 662 555 255 11 789 3423 Fragments arranged by size: 3423 789 662 555 255 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2102 2122 4282 4656 4802 387 Size: 1064 138 513 20 2160 374 146 1280 Fragments arranged by size: 2160 1280 1064 513 374 146 138 20 EcoICRI GAG'CTC Cuts at: 2025 2025 Size: 5695 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2567 2820 3758 4355 5142 5410 1239 Size: 393 935 253 938 597 787 268 1524 Fragments arranged by size: 1524 938 935 787 597 393 268 253 EcoNI CCTnn'n_nnAGG Cuts at: 2237 2237 Size: 5695 EcoO109I rG'GnC_Cy Cuts at: 2643 2643 Size: 5695 EcoRI G'AATT_C Cuts at: 3004 3382 3004 Size: 378 5317 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2044 2044 Size: 5695 FspI TGC'GCA Cuts at: 2846 4201 2846 Size: 1355 4340 HaeII r_GCGC'y Cuts at: 1678 2326 2659 4842 1678 Size: 648 333 2183 2531 Fragments arranged by size: 2531 2183 648 333 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2198 2230 2377 2409 4336 Size: 32 135 32 626 32 147 32 1927 Cuts at: 4336 4368 4410 4442 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1927 626 147 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3039 3285 669 Size: 988 281 1101 246 3079 Fragments arranged by size: 3079 1101 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5695 HpaI GTT'AAC Cuts at: 3285 3285 Size: 5695 KasI G'GCGC_C Cuts at: 2655 2655 Size: 5695 KpnI G_GTAC'C Cuts at: 2233 3020 2233 Size: 787 4908 MfeI C'AATT_G Cuts at: 3294 3294 Size: 5695 MluI A'CGCG_T Cuts at: 5571 5571 Size: 5695 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2502 4809 4993 1305 Size: 1197 2307 184 2007 Fragments arranged by size: 2307 2007 1197 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3873 Fragments arranged by size: 3873 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2432 2576 3653 4012 4171 518 Size: 648 1266 144 1077 359 159 2042 Fragments arranged by size: 2042 1266 1077 648 359 159 144 MspA1I CmG'CkG Cuts at: 1606 2226 3111 3788 4936 5181 1606 Size: 620 885 677 1148 245 2120 Fragments arranged by size: 2120 1148 885 677 620 245 NaeI GCC'GGC Cuts at: 2369 2369 Size: 5695 NarI GG'CG_CC Cuts at: 2656 2656 Size: 5695 NcoI C'CATG_G Cuts at: 513 1065 2577 513 Size: 552 1512 3631 Fragments arranged by size: 3631 1512 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5695 NgoMIV G'CCGG_C Cuts at: 2367 2367 Size: 5695 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5695 NotI GC'GGCC_GC Cuts at: 3142 3142 Size: 5695 NspI r_CATG'y Cuts at: 1132 1229 3053 4598 1132 Size: 97 1824 1545 2229 Fragments arranged by size: 2229 1824 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5695 PciI A'CATG_T Cuts at: 4594 4594 Size: 5695 PflMI CCAn_nnn'nTGG Cuts at: 1108 2734 1108 Size: 1626 4069 PmeI GTTT'AAAC Cuts at: 2999 2999 Size: 5695 PmlI CAC'GTG Cuts at: 2295 2295 Size: 5695 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3764 3796 5277 5309 3764 Size: 32 1481 32 4150 Fragments arranged by size: 4150 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5695 PsiI TTA'TAA Cuts at: 3265 3265 Size: 5695 PspOMI G'GGCC_C Cuts at: 2643 2643 Size: 5695 PstI C_TGCA'G Cuts at: 830 2291 2871 3047 830 Size: 1461 580 176 3478 Fragments arranged by size: 3478 1461 580 176 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5695 SacI G_AGCT'C Cuts at: 2027 2027 Size: 5695 SalI G'TCGA_C Cuts at: 1936 3037 1936 Size: 1101 4594 SapI GCTCTTCn'nnn_ Cuts at: 2015 2570 2825 2015 Size: 555 255 4885 Fragments arranged by size: 4885 555 255 SbfI CC_TGCA'GG Cuts at: 3047 3047 Size: 5695 ScaI AGT'ACT Cuts at: 3943 5655 3943 Size: 1712 3983 SexAI A'CCwGG_T Cuts at: 2730 2730 Size: 5695 SfcI C'TryA_G Cuts at: 826 1045 2272 2287 2867 2988 3043 4178 4859 Size: 219 1227 15 580 121 55 1135 681 Cuts at: 4859 5050 826 Size: 191 1471 Fragments arranged by size: 1471 1227 1135 681 580 219 191 121 55 15 SfiI GGCCn_nnn'nGGCC Cuts at: 2093 2093 Size: 5695 SfoI GGC'GCC Cuts at: 2657 2657 Size: 5695 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5695 SmaI CCC'GGG Cuts at: 1872 3022 1872 Size: 1150 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3799 4700 4962 820 Size: 197 926 1856 901 262 1553 Fragments arranged by size: 1856 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3486 493 Size: 2993 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5695 SphI G_CATG'C Cuts at: 1132 3053 1132 Size: 1921 3774 SspI AAT'ATT Cuts at: 5 52 3619 5 Size: 47 3567 2081 Fragments arranged by size: 3567 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5695 StyI C'CwwG_G Cuts at: 513 1065 2238 2577 3134 513 Size: 552 1173 339 557 3074 Fragments arranged by size: 3074 1173 557 552 339 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3722 3739 3892 4077 4522 325 Size: 1255 2142 17 153 185 445 1498 Fragments arranged by size: 2142 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2352 3941 5653 Size: 274 80 33 51 1443 375 1589 1712 Cuts at: 5653 96 Size: 138 Fragments arranged by size: 1712 1589 1443 375 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5655 5687 5655 Size: 32 5663 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5695 XbaI T'CTAG_A Cuts at: 3031 3031 Size: 5695 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5695 XmaI C'CCGG_G Cuts at: 1870 3020 1870 Size: 1150 4545 XmnI GAAnn'nnTTC Cuts at: 3824 3824 Size: 5695 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3503 276 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PfoI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SrfI SwaI XcmI