Restriction map for
pFN21AB9826
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwNI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BbrPI BbsI BbvCI BciVI
BfrI BglII BlnI BpuAI BsaI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrDI BsrFI BsrGI BsrI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Cfr10I ClaI CpoI DraI
DraIII DrdI Eam1105I EarI EcoO65I
EcoRI EcoRV EcoT22I FseI HincII
HindII HindIII HinfI HpaI KpnI
Ksp632I MaeIII MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI SwaI TaqI
TfiI Tsp45I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
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AcyI 610 GGCGCC G[GA]^CG[CT]C
Alw44I 64 GTGCAC G^TGCAC
AocI 462 CCTGAGG CC^T[ACGT]AGG
Aor51HI 507 AGCGCT AGC^GCT
ApaI 264 GGGCCC GGGCC^C
ApaLI 64 GTGCAC G^TGCAC
AvaII 336 GGACC G^G[AT]CC
AviII 641 TGCGCA TGC^GCA
BalI 836 TGGCCA TGG^CCA
BbeI 610 GGCGCC GGCGC^C
BcgI 200 CGAAGGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 350 TGATCA T^GATCA
BfaI 136 CTAG C^TAG
BglI 235 GCCCACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 540 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 540 GCTGAGC GC^T[ACGT]AGC
BsaHI 610 GGCGCC G[GA]^CG[CT]C
BsiEI 146 CGGCCG CG[GA][CT]^CG
BsmAI 952 GTCTC GTCTC(1/5)
BspHI 575 TCATGA T^CATGA
BsrBI 149 CCGCTC CCGCTC(-3/-3)
BssSI 307 CACGAG CACGAG(-5/-1)
BstAPI 288 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 18 GGATCT [GA]^GATC[CT]
Bsu36I 462 CCTGAGG CC^T[ACGT]AGG
CelII 540 GCTGAGC GC^T[ACGT]AGC
EagI 146 CGGCCG C^GGCCG
EclXI 146 CGGCCG C^GGCCG
Eco47III 507 AGCGCT AGC^GCT
Eco52I 146 CGGCCG C^GGCCG
Eco81I 462 CCTGAGG CC^T[ACGT]AGG
EcoT14I 485 CCATGG C^C[AT][AT]GG
FauI 912 GCGGG CCCGC(4/6)
FbaI 350 TGATCA T^GATCA
FspI 641 TGCGCA TGC^GCA
HgaI 746 GCGTC GACGC(5/10)
Hin1I 610 GGCGCC G[GA]^CG[CT]C
KasI 610 GGCGCC G^GCGCC
KspI 606 CCGCGG CCGC^GG
MaeI 136 CTAG C^TAG
MflI 18 GGATCT [GA]^GATC[CT]
MluNI 836 TGGCCA TGG^CCA
MscI 836 TGGCCA TGG^CCA
MseI 848 TTAA T^TAA
NarI 610 GGCGCC GG^CGCC
NcoI 485 CCATGG C^CATGG
NspI 551 GCATGC [GA]CATG^[CT]
PstI 208 CTGCAG CTGCA^G
PvuII 289 CAGCTG CAG^CTG
RcaI 575 TCATGA T^CATGA
SacII 606 CCGCGG CCGC^GG
SfcI 208 CTGCAG C^T[GA][CT]AG
SfoI 610 GGCGCC GGC^GCC
SphI 551 GCATGC GCATG^C
StyI 485 CCATGG C^C[AT][AT]GG
Tru9I 848 TTAA T^TAA
Tsp509I 727 AATT ^AATT
XhoII 18 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 321 GTAC GT^AC
738 GTAC
AlwI 579 GATCC GGATC(4/5)
18 GGATC
BanI 270 GGCACC G^G[CT][GA]CC
610 GGCGCC
BpmI 511 CTGGAG CTGGAG(16/14)
892 CTGGAG
BsaBI 348 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
816 GATGCAAATC
BsmFI 686 GGGAC GGGAC(10/14)
748 GTCCC
DsaI 485 CCATGG C^C[GA][CT]GG
606 CCGCGG
EaeI 146 CGGCCG [CT]^GGCC[GA]
836 TGGCCA
EcoNI 130 CCTCCTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
207 CCTGCAGCAGG
HphI 372 GGTGA GGTGA(8/7)
809 TCACC
MamI 348 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
816 GATGCAAATC
MspA1I 289 CAGCTG C[AC]G^C[GT]G
606 CCGCGG
RsaI 321 GTAC GT^AC
738 GTAC
SacI 310 GAGCTC GAGCT^C
905 GAGCTC
SmaI 443 CCCGGG CCC^GGG
791 CCCGGG
StuI 564 AGGCCT AGG^CCT
863 AGGCCT
XmaCI 443 CCCGGG C^CCGGG
791 CCCGGG
XmaI 443 CCCGGG C^CCGGG
791 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
BanII BbvI BcnI BmyI BsaAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsoBI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII ItaI MaeII
MboI MboII MnlI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TseI TspRI