(Circular) (Six-base) MAPSORT of: pFN21AB9826.seq Check: 1145 from: 1 to: 5668 pFN21AB9826 5668 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3478 278 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3011 1937 Size: 1074 4594 Acc65I G'GTAC_C Cuts at: 2989 2989 Size: 5668 AclI AA'CG_TT Cuts at: 1563 3795 4168 1563 Size: 2232 373 3063 Fragments arranged by size: 3063 2232 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2759 3731 5115 5383 1632 Size: 1127 972 1384 268 1917 Fragments arranged by size: 1917 1384 1127 972 268 AfeI AGC'GCT Cuts at: 2514 2514 Size: 5668 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5471 AflIII A'CryG_T Cuts at: 1203 4567 5544 1203 Size: 3364 977 1327 Fragments arranged by size: 3364 1327 977 AgeI A'CCGG_T Cuts at: 4499 4499 Size: 5668 AhdI GACnn_n'nnGTC Cuts at: 4397 4397 Size: 5668 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5668 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2238 2573 1184 Size: 1054 335 4279 Fragments arranged by size: 4279 1054 335 AlwNI CAG_nnn'CTG Cuts at: 3411 4983 3411 Size: 1572 4096 ApaI G_GGCC'C Cuts at: 2273 2273 Size: 5668 ApaLI G'TGCA_C Cuts at: 2069 3725 4881 2069 Size: 1656 1156 2856 Fragments arranged by size: 2856 1656 1156 ApoI r'AATT_y Cuts at: 1493 2977 3203 3355 5464 1493 Size: 1484 226 152 2109 1697 Fragments arranged by size: 2109 1697 1484 226 152 AseI AT'TA_AT Cuts at: 160 1032 4222 160 Size: 872 3190 1606 Fragments arranged by size: 3190 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 AvaI C'yCGr_G Cuts at: 1870 1943 2251 2448 2522 2796 2993 1870 Size: 73 308 197 74 274 197 4545 Fragments arranged by size: 4545 308 274 197 197 74 73 BamHI G'GATC_C Cuts at: 2998 3365 2998 Size: 367 5301 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2275 2615 2989 4444 618 Size: 325 217 627 488 340 374 1455 1842 Fragments arranged by size: 1842 1455 627 488 374 340 325 217 BanII G_rGCy'C Cuts at: 1391 2135 2273 2319 2523 2795 2914 2987 1391 Size: 744 138 46 204 272 119 73 4072 Fragments arranged by size: 4072 744 272 204 138 119 73 46 BbeI G_GCGC'C Cuts at: 2619 2619 Size: 5668 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4697 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2194 2228 3859 3893 1903 Size: 34 257 34 1631 34 3678 Fragments arranged by size: 3678 1631 257 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3560 4770 3560 Size: 1210 4458 BclI T'GATC_A Cuts at: 1548 2355 1548 Size: 807 4861 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2246 4279 136 Size: 107 122 71 631 605 574 2033 1525 Fragments arranged by size: 2033 1525 631 605 574 122 107 71 BglII A'GATC_T Cuts at: 5663 5663 Size: 5668 BlpI GC'TnA_GC Cuts at: 2546 3085 2546 Size: 539 5129 Bme1580I G_kGCm'C Cuts at: 1790 2073 2273 3729 4885 1790 Size: 283 200 1456 1156 2573 Fragments arranged by size: 2573 1456 1156 283 200 BmgBI CAC'GTC Cuts at: 1318 2695 1318 Size: 1377 4291 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3399 4357 442 Size: 416 53 83 399 24 1982 958 1753 Fragments arranged by size: 1982 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5668 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2154 2154 Size: 5668 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2537 2918 4328 1239 Size: 1298 381 1410 2579 Fragments arranged by size: 2579 1410 1298 381 Bpu10I CC'TnA_GC Cuts at: 2588 2588 Size: 5668 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3793 4658 4956 3793 Size: 865 298 4505 Fragments arranged by size: 4505 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4331 882 Size: 617 2832 2219 Fragments arranged by size: 2832 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2329 2602 2761 3459 493 Size: 711 1125 273 159 698 2702 Fragments arranged by size: 2702 1125 711 698 273 159 BsaBI GATnn'nnATC Cuts at: 2357 2825 5662 2357 Size: 468 2837 2363 Fragments arranged by size: 2837 2363 468 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2616 3475 3857 4492 Size: 53 83 186 936 1083 859 382 635 Cuts at: 4492 275 Size: 1451 Fragments arranged by size: 1451 1083 936 859 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4101 4499 4773 4920 5331 4101 Size: 398 274 147 411 4438 Fragments arranged by size: 4438 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2140 2170 1178 Size: 30 165 30 737 30 4676 Fragments arranged by size: 4676 737 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2127 2243 2540 2729 2915 1186 Size: 941 116 297 189 186 3939 Fragments arranged by size: 3939 941 297 189 186 116 BseYI C'CCAG_C Cuts at: 2262 4871 5367 2262 Size: 2609 496 2563 Fragments arranged by size: 2609 2563 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2321 2431 2447 2468 1163 Size: 573 585 110 16 21 4363 Fragments arranged by size: 4363 585 573 110 21 16 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2154 3118 3879 4907 1540 Size: 275 186 153 964 761 1028 2301 Fragments arranged by size: 2301 1028 964 761 275 186 153 BsiHKAI G_wGCw'C Cuts at: 2073 2319 2914 3729 3814 4885 2073 Size: 246 595 815 85 1071 2856 Fragments arranged by size: 2856 1071 815 595 246 85 BsmI GAATG_Cn' Cuts at: 3179 3272 3179 Size: 93 5575 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2135 2273 2319 2523 2795 2914 Size: 399 283 62 138 46 204 272 119 Cuts at: 2914 2987 3729 3814 4885 1391 Size: 73 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 283 272 204 138 119 85 73 62 46 BspEI T'CCGG_A Cuts at: 5331 5331 Size: 5668 BspHI T'CATG_A Cuts at: 2580 3557 5287 2580 Size: 977 1730 2961 Fragments arranged by size: 2961 1730 977 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3023 844 Size: 2179 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2156 3555 1394 Size: 429 333 1399 3507 Fragments arranged by size: 3507 1399 429 333 BsrDI GCAATG_nn' Cuts at: 65 1248 4163 4337 65 Size: 1183 2915 174 1396 Fragments arranged by size: 2915 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4312 4499 1700 Size: 110 2502 187 2869 Fragments arranged by size: 2869 2502 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5668 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5668 BssSI C'ACGA_G Cuts at: 2312 3728 4740 2312 Size: 1416 1012 3240 Fragments arranged by size: 3240 1416 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2299 794 Size: 1505 4163 BstBI TT'CG_AA Cuts at: 4472 4472 Size: 5668 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5668 BstYI r'GATC_y Cuts at: 1972 2023 2998 3365 3750 3767 5219 5305 5317 Size: 51 975 367 385 17 1452 86 12 Cuts at: 5317 5663 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 975 385 367 346 86 51 17 12 Bsu36I CC'TnA_GG Cuts at: 2468 2468 Size: 5668 BtgI C'CryG_G Cuts at: 513 1065 2490 2611 513 Size: 552 1425 121 3570 Fragments arranged by size: 3570 1425 552 121 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4929 Fragments arranged by size: 4929 683 56 BtsI GCAGTG_nn' Cuts at: 2931 2959 3184 3978 3998 2931 Size: 28 225 794 20 4601 Fragments arranged by size: 4601 794 225 28 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5668 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5633 DraI TTT'AAA Cuts at: 2972 2972 Size: 5668 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4675 809 Size: 507 3359 1802 Fragments arranged by size: 3359 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2151 2841 3115 Size: 54 1425 50 275 18 321 690 274 Cuts at: 3115 4004 5633 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 690 321 275 274 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2151 3115 1537 Size: 275 339 964 4090 Fragments arranged by size: 4090 964 339 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3598 1353 Size: 2245 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4255 4629 4775 387 Size: 1064 138 2666 374 146 1280 Fragments arranged by size: 2666 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2317 2912 2317 Size: 595 5073 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2537 2759 2918 3731 4328 5115 5383 Size: 393 905 222 159 813 597 787 268 Cuts at: 5383 1239 Size: 1524 Fragments arranged by size: 1524 905 813 787 597 393 268 222 159 EcoNI CCTnn'n_nnAGG Cuts at: 2139 2216 2139 Size: 77 5591 EcoO109I rG'GnC_Cy Cuts at: 2053 2182 2209 2248 2053 Size: 129 27 39 5473 Fragments arranged by size: 5473 129 39 27 EcoRI G'AATT_C Cuts at: 2977 3355 2977 Size: 378 5290 FspI TGC'GCA Cuts at: 2648 4174 2648 Size: 1526 4142 HaeII r_GCGC'y Cuts at: 1678 2516 2546 2619 2631 4815 1678 Size: 838 30 73 12 2184 2531 Fragments arranged by size: 2531 2184 838 73 30 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2140 2172 4309 4341 4383 Size: 32 135 32 568 32 2137 32 42 Cuts at: 4383 4415 1373 Size: 32 2626 Fragments arranged by size: 2626 2137 568 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3012 3258 669 Size: 988 281 1074 246 3079 Fragments arranged by size: 3079 1074 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5668 HpaI GTT'AAC Cuts at: 3258 3258 Size: 5668 KasI G'GCGC_C Cuts at: 2615 2615 Size: 5668 KpnI G_GTAC'C Cuts at: 2993 2993 Size: 5668 MfeI C'AATT_G Cuts at: 3267 3267 Size: 5668 MluI A'CGCG_T Cuts at: 5544 5544 Size: 5668 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4782 4966 1305 Size: 3477 184 2007 Fragments arranged by size: 3477 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2843 10 Size: 54 1425 343 1011 2835 Fragments arranged by size: 2835 1425 1011 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2561 2600 2819 3626 3985 4144 518 Size: 648 1395 39 219 807 359 159 2042 Fragments arranged by size: 2042 1395 807 648 359 219 159 39 MspA1I CmG'CkG Cuts at: 1606 2296 2613 3084 3761 4909 5154 1606 Size: 690 317 471 677 1148 245 2120 Fragments arranged by size: 2120 1148 690 677 471 317 245 NarI GG'CG_CC Cuts at: 2616 2616 Size: 5668 NcoI C'CATG_G Cuts at: 513 1065 2490 513 Size: 552 1425 3691 Fragments arranged by size: 3691 1425 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5668 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5668 NotI GC'GGCC_GC Cuts at: 3115 3115 Size: 5668 NspI r_CATG'y Cuts at: 1132 1229 2560 3026 4571 1132 Size: 97 1331 466 1545 2229 Fragments arranged by size: 2229 1545 1331 466 97 PasI CC'CwG_GG Cuts at: 1348 2081 1348 Size: 733 4935 PciI A'CATG_T Cuts at: 4567 4567 Size: 5668 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5668 PmeI GTTT'AAAC Cuts at: 2972 2972 Size: 5668 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3737 3769 5250 5282 3737 Size: 32 1481 32 4123 Fragments arranged by size: 4123 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5668 PsiI TTA'TAA Cuts at: 3238 3238 Size: 5668 PspOMI G'GGCC_C Cuts at: 2269 2269 Size: 5668 PstI C_TGCA'G Cuts at: 830 2217 3020 830 Size: 1387 803 3478 Fragments arranged by size: 3478 1387 803 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5668 PvuII CAG'CTG Cuts at: 2296 2296 Size: 5668 SacI G_AGCT'C Cuts at: 2319 2914 2319 Size: 595 5073 SacII CC_GC'GG Cuts at: 2614 2614 Size: 5668 SalI G'TCGA_C Cuts at: 1936 3010 1936 Size: 1074 4594 SbfI CC_TGCA'GG Cuts at: 3020 3020 Size: 5668 ScaI AGT'ACT Cuts at: 3916 5628 3916 Size: 1712 3956 SfcI C'TryA_G Cuts at: 826 1045 2213 3016 4151 4832 5023 826 Size: 219 1168 803 1135 681 191 1471 Fragments arranged by size: 1471 1168 1135 803 681 219 191 SfoI GGC'GCC Cuts at: 2617 2617 Size: 5668 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5668 SmaI CCC'GGG Cuts at: 1872 2450 2798 2995 1872 Size: 578 348 197 4545 Fragments arranged by size: 4545 578 348 197 SmlI C'TyrA_G Cuts at: 820 1017 1943 3772 4673 4935 820 Size: 197 926 1829 901 262 1553 Fragments arranged by size: 1829 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3459 493 Size: 2966 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5668 SphI G_CATG'C Cuts at: 1132 2560 3026 1132 Size: 1428 466 3774 Fragments arranged by size: 3774 1428 466 SspI AAT'ATT Cuts at: 5 52 3592 5 Size: 47 3540 2081 Fragments arranged by size: 3540 2081 47 StuI AGG'CCT Cuts at: 1517 2571 2870 1517 Size: 1054 299 4315 Fragments arranged by size: 4315 1054 299 StyI C'CwwG_G Cuts at: 513 1065 2490 3107 513 Size: 552 1425 617 3074 Fragments arranged by size: 3074 1425 617 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3695 3712 3865 4050 4495 325 Size: 1255 2115 17 153 185 445 1498 Fragments arranged by size: 2115 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3914 5626 96 Size: 274 80 33 51 1443 1937 1712 138 Fragments arranged by size: 1937 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5628 5660 5628 Size: 32 5636 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5668 XbaI T'CTAG_A Cuts at: 3004 3004 Size: 5668 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5668 XmaI C'CCGG_G Cuts at: 1870 2448 2796 2993 1870 Size: 578 348 197 4545 Fragments arranged by size: 4545 578 348 197 XmnI GAAnn'nnTTC Cuts at: 3797 3797 Size: 5668 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3476 276 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI