Restriction map for
pFN21AB9826
(Linear) (Six-base) MAPSORT of: KIBB9826.seq Check: 9129 from: 1 to: 963
KIBB9826.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 963
Size: 754 209
AfeI AGC'GCT
Cuts at: 0 509 963
Size: 509 454
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 233 568 963
Size: 233 335 395
Fragments arranged by size:
395 335 233
ApaI G_GGCC'C
Cuts at: 0 268 963
Size: 268 695
ApaLI G'TGCA_C
Cuts at: 0 64 963
Size: 64 899
AvaI C'yCGr_G
Cuts at: 0 246 443 517 791 963
Size: 246 197 74 274 172
Fragments arranged by size:
274 246 197 172 74
BanI G'GyrC_C
Cuts at: 0 270 610 963
Size: 270 340 353
Fragments arranged by size:
353 340 270
BanII G_rGCy'C
Cuts at: 0 130 268 314 518 790 909 963
Size: 130 138 46 204 272 119 54
Fragments arranged by size:
272 204 138 130 119 54 46
BbeI G_GCGC'C
Cuts at: 0 614 963
Size: 614 349
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 189 223 963
Size: 189 34 740
Fragments arranged by size:
740 189 34
BclI T'GATC_A
Cuts at: 0 350 963
Size: 350 613
BglI GCCn_nnn'nGGC
Cuts at: 0 241 963
Size: 241 722
BlpI GC'TnA_GC
Cuts at: 0 541 963
Size: 541 422
Bme1580I G_kGCm'C
Cuts at: 0 68 268 963
Size: 68 200 695
Fragments arranged by size:
695 200 68
BmgBI CAC'GTC
Cuts at: 0 690 963
Size: 690 273
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 149 963
Size: 149 814
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 913 963
Size: 532 381 50
Fragments arranged by size:
532 381 50
Bpu10I CC'TnA_GC
Cuts at: 0 583 963
Size: 583 380
BsaAI yAC'GTr
Cuts at: 0 324 597 756 963
Size: 324 273 159 207
Fragments arranged by size:
324 273 207 159
BsaBI GATnn'nnATC
Cuts at: 0 352 820 963
Size: 352 468 143
Fragments arranged by size:
468 352 143
BsaHI Gr'CG_yC
Cuts at: 0 611 963
Size: 611 352
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 135 165 963
Size: 135 30 798
Fragments arranged by size:
798 135 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 122 238 535 724 910 963
Size: 122 116 297 189 186 53
Fragments arranged by size:
297 189 186 122 116 53
BseYI C'CCAG_C
Cuts at: 0 257 963
Size: 257 706
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 426 442 463 963
Size: 316 110 16 21 500
Fragments arranged by size:
500 316 110 21 16
BsiEI CG_ry'CG
Cuts at: 0 149 963
Size: 149 814
BsiHKAI G_wGCw'C
Cuts at: 0 68 314 909 963
Size: 68 246 595 54
Fragments arranged by size:
595 246 68 54
Bsp1286I G_dGCh'C
Cuts at: 0 68 130 268 314 518 790 909 963
Size: 68 62 138 46 204 272 119 54
Fragments arranged by size:
272 204 138 119 68 62 54 46
BspHI T'CATG_A
Cuts at: 0 575 963
Size: 575 388
BsrBI CCG'CTC
Cuts at: 0 151 963
Size: 151 812
BssSI C'ACGA_G
Cuts at: 0 307 963
Size: 307 656
BstAPI GCAn_nnn'nTGC
Cuts at: 0 294 963
Size: 294 669
BstYI r'GATC_y
Cuts at: 0 18 963
Size: 18 945
Bsu36I CC'TnA_GG
Cuts at: 0 463 963
Size: 463 500
BtgI C'CryG_G
Cuts at: 0 485 606 963
Size: 485 121 357
Fragments arranged by size:
485 357 121
BtsI GCAGTG_nn'
Cuts at: 0 926 954 963
Size: 926 28 9
Fragments arranged by size:
926 28 9
EaeI y'GGCC_r
Cuts at: 0 146 836 963
Size: 146 690 127
Fragments arranged by size:
690 146 127
EagI C'GGCC_G
Cuts at: 0 146 963
Size: 146 817
EcoICRI GAG'CTC
Cuts at: 0 312 907 963
Size: 312 595 56
Fragments arranged by size:
595 312 56
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 754 913 963
Size: 532 222 159 50
Fragments arranged by size:
532 222 159 50
EcoNI CCTnn'n_nnAGG
Cuts at: 0 134 211 963
Size: 134 77 752
Fragments arranged by size:
752 134 77
EcoO109I rG'GnC_Cy
Cuts at: 0 48 177 204 243 963
Size: 48 129 27 39 720
Fragments arranged by size:
720 129 48 39 27
FspI TGC'GCA
Cuts at: 0 643 963
Size: 643 320
HaeII r_GCGC'y
Cuts at: 0 511 541 614 626 963
Size: 511 30 73 12 337
Fragments arranged by size:
511 337 73 30 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 135 167 963
Size: 135 32 796
Fragments arranged by size:
796 135 32
KasI G'GCGC_C
Cuts at: 0 610 963
Size: 610 353
MscI TGG'CCA
Cuts at: 0 838 963
Size: 838 125
MslI CAynn'nnrTG
Cuts at: 0 556 595 814 963
Size: 556 39 219 149
Fragments arranged by size:
556 219 149 39
MspA1I CmG'CkG
Cuts at: 0 291 608 963
Size: 291 317 355
Fragments arranged by size:
355 317 291
NarI GG'CG_CC
Cuts at: 0 611 963
Size: 611 352
NcoI C'CATG_G
Cuts at: 0 485 963
Size: 485 478
NspI r_CATG'y
Cuts at: 0 555 963
Size: 555 408
PasI CC'CwG_GG
Cuts at: 0 76 963
Size: 76 887
PspOMI G'GGCC_C
Cuts at: 0 264 963
Size: 264 699
PstI C_TGCA'G
Cuts at: 0 212 963
Size: 212 751
PvuII CAG'CTG
Cuts at: 0 291 963
Size: 291 672
SacI G_AGCT'C
Cuts at: 0 314 909 963
Size: 314 595 54
Fragments arranged by size:
595 314 54
SacII CC_GC'GG
Cuts at: 0 609 963
Size: 609 354
SfcI C'TryA_G
Cuts at: 0 208 963
Size: 208 755
SfoI GGC'GCC
Cuts at: 0 612 963
Size: 612 351
SmaI CCC'GGG
Cuts at: 0 445 793 963
Size: 445 348 170
Fragments arranged by size:
445 348 170
SphI G_CATG'C
Cuts at: 0 555 963
Size: 555 408
StuI AGG'CCT
Cuts at: 0 566 865 963
Size: 566 299 98
Fragments arranged by size:
566 299 98
StyI C'CwwG_G
Cuts at: 0 485 963
Size: 485 478
XmaI C'CCGG_G
Cuts at: 0 443 791 963
Size: 443 348 172
Fragments arranged by size:
443 348 172
Enzymes that do cut:
AcuI AfeI AlfI ApaI ApaLI AvaI BanI BanII
BbeI BcgI BclI BglI BlpI Bme1580I BmgBI BplI
BpmI Bpu10I BsaAI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BsrBI BssSI BstAPI
BstYI Bsu36I BtgI BtsI EaeI EagI EcoICRI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I KasI MscI MslI
MspA1I NarI NcoI NspI PasI PspOMI PstI PvuII
SacI SacII SfcI SfoI SmaI SphI StuI StyI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BciVI BfrBI BglII
BmrI BmtI BpuEI BsaI BsaWI BsiWI BsmI BsmBI
BspEI BspMI BsrDI BsrFI BsrGI BssHII BstBI BstEII
BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EarI EciI EcoRI EcoRV FalI FseI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI
SspI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmnI ZraI