(Circular) (Six-base) MAPSORT of: pFN21AB9824.seq Check: 4336 from: 1 to: 5650 pFN21AB9824 5650 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3460 278 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2993 1937 Size: 1056 4594 Acc65I G'GTAC_C Cuts at: 2971 2971 Size: 5650 AclI AA'CG_TT Cuts at: 1563 3777 4150 1563 Size: 2214 373 3063 Fragments arranged by size: 3063 2214 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3713 5097 5365 1632 Size: 2081 1384 268 1917 Fragments arranged by size: 2081 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2492 820 Size: 197 1475 3978 Fragments arranged by size: 3978 1475 197 AflIII A'CryG_T Cuts at: 1203 2119 4549 5526 1203 Size: 916 2430 977 1327 Fragments arranged by size: 2430 1327 977 916 AgeI A'CCGG_T Cuts at: 4481 4481 Size: 5650 AhdI GACnn_n'nnGTC Cuts at: 4379 4379 Size: 5650 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5650 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5650 AlwNI CAG_nnn'CTG Cuts at: 2407 3393 4965 2407 Size: 986 1572 3092 Fragments arranged by size: 3092 1572 986 ApaLI G'TGCA_C Cuts at: 2569 3707 4863 2569 Size: 1138 1156 3356 Fragments arranged by size: 3356 1156 1138 ApoI r'AATT_y Cuts at: 1493 2959 3185 3337 5446 1493 Size: 1466 226 152 2109 1697 Fragments arranged by size: 2109 1697 1466 226 152 AseI AT'TA_AT Cuts at: 160 1032 4204 160 Size: 872 3172 1606 Fragments arranged by size: 3172 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 AvaI C'yCGr_G Cuts at: 1870 1943 2975 1870 Size: 73 1032 4545 Fragments arranged by size: 4545 1032 73 AvrII C'CTAG_G Cuts at: 2279 2927 2279 Size: 648 5002 BamHI G'GATC_C Cuts at: 2980 3347 2980 Size: 367 5283 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2971 4426 618 Size: 325 217 627 1184 1455 1842 Fragments arranged by size: 1842 1455 1184 627 325 217 BanII G_rGCy'C Cuts at: 1391 2020 2088 2205 2320 2478 2969 1391 Size: 629 68 117 115 158 491 4072 Fragments arranged by size: 4072 629 491 158 117 115 68 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4679 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3841 3875 1903 Size: 34 1904 34 3678 Fragments arranged by size: 3678 1904 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3542 4752 3542 Size: 1210 4440 BclI T'GATC_A Cuts at: 1548 1548 Size: 5650 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2281 4261 Size: 107 122 71 631 605 337 272 1980 Cuts at: 4261 136 Size: 1525 Fragments arranged by size: 1980 1525 631 605 337 272 122 107 71 BglII A'GATC_T Cuts at: 5645 5645 Size: 5650 BlpI GC'TnA_GC Cuts at: 3067 3067 Size: 5650 Bme1580I G_kGCm'C Cuts at: 1790 2573 3711 4867 1790 Size: 783 1138 1156 2573 Fragments arranged by size: 2573 1156 1138 783 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5650 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3381 4339 442 Size: 416 53 83 399 24 1964 958 1753 Fragments arranged by size: 1964 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5650 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2120 2120 Size: 5650 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2270 4310 1239 Size: 1031 2040 2579 Fragments arranged by size: 2579 2040 1031 Bpu10I CC'TnA_GC Cuts at: 2262 2350 2262 Size: 88 5562 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2189 2462 3775 4640 4938 2189 Size: 273 1313 865 298 2901 Fragments arranged by size: 2901 1313 865 298 273 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2681 4313 882 Size: 617 1182 1632 2219 Fragments arranged by size: 2219 1632 1182 617 BsaAI yAC'GTr Cuts at: 493 1204 3441 493 Size: 711 2237 2702 Fragments arranged by size: 2702 2237 711 BsaBI GATnn'nnATC Cuts at: 5644 5644 Size: 5650 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2054 2100 3457 3839 Size: 53 83 186 936 521 46 1357 382 Cuts at: 3839 4474 275 Size: 635 1451 Fragments arranged by size: 1451 1357 936 635 521 382 186 83 53 46 BsaWI w'CCGG_w Cuts at: 4083 4481 4755 4902 5313 4083 Size: 398 274 147 411 4420 Fragments arranged by size: 4420 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5425 Fragments arranged by size: 5425 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2205 2383 2416 1186 Size: 1019 178 33 4420 Fragments arranged by size: 4420 1019 178 33 BseYI C'CCAG_C Cuts at: 2109 4853 5349 2109 Size: 2744 496 2410 Fragments arranged by size: 2744 2410 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2295 2507 1163 Size: 573 559 212 4306 Fragments arranged by size: 4306 573 559 212 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3100 3861 4889 1540 Size: 275 186 1099 761 1028 2301 Fragments arranged by size: 2301 1099 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2020 2268 2478 2573 3711 3796 4867 2020 Size: 248 210 95 1138 85 1071 2803 Fragments arranged by size: 2803 1138 1071 248 210 95 85 BsmI GAATG_Cn' Cuts at: 3161 3254 3161 Size: 93 5557 BsmBI CGTCTCn'nnnn_ Cuts at: 2091 2091 Size: 5650 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2020 2088 2205 2268 2320 2478 2573 Size: 399 230 68 117 63 52 158 95 Cuts at: 2573 2969 3711 3796 4867 1391 Size: 396 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 396 230 158 117 95 85 68 63 52 BspEI T'CCGG_A Cuts at: 5313 5313 Size: 5650 BspHI T'CATG_A Cuts at: 3539 5269 3539 Size: 1730 3920 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2104 2287 3005 844 Size: 1260 183 718 3489 Fragments arranged by size: 3489 1260 718 183 BsrBI CCG'CTC Cuts at: 1394 1823 3537 1394 Size: 429 1714 3507 Fragments arranged by size: 3507 1714 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4145 4319 65 Size: 1183 2897 174 1396 Fragments arranged by size: 2897 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4294 4481 1700 Size: 110 2484 187 2869 Fragments arranged by size: 2869 2484 187 110 BsrGI T'GTAC_A Cuts at: 96 2753 96 Size: 2657 2993 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5650 BssSI C'ACGA_G Cuts at: 2240 2313 3710 4722 2240 Size: 73 1397 1012 3168 Fragments arranged by size: 3168 1397 1012 73 BstAPI GCAn_nnn'nTGC Cuts at: 794 2044 794 Size: 1250 4400 BstBI TT'CG_AA Cuts at: 4454 4454 Size: 5650 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5650 BstXI CCAn_nnnn'nTGG Cuts at: 2598 2598 Size: 5650 BstYI r'GATC_y Cuts at: 1972 2330 2601 2980 3347 3732 3749 5201 5287 Size: 358 271 379 367 385 17 1452 86 Cuts at: 5287 5299 5645 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 379 367 358 346 271 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2251 2769 513 Size: 552 939 247 518 3394 Fragments arranged by size: 3394 939 552 518 247 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4911 Fragments arranged by size: 4911 683 56 BtsI GCAGTG_nn' Cuts at: 2621 3166 3960 3980 2621 Size: 545 794 20 4291 Fragments arranged by size: 4291 794 545 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5650 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5615 DraI TTT'AAA Cuts at: 2954 2954 Size: 5650 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2253 4657 809 Size: 507 937 2404 1802 Fragments arranged by size: 2404 1802 937 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3097 3986 5615 Size: 54 1425 50 275 18 1267 889 1629 Cuts at: 5615 8 Size: 43 Fragments arranged by size: 1629 1425 1267 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3097 1537 Size: 275 1285 4090 Fragments arranged by size: 4090 1285 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2684 3580 1353 Size: 1331 896 3423 Fragments arranged by size: 3423 1331 896 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4237 4611 4757 387 Size: 1064 138 2648 374 146 1280 Fragments arranged by size: 2648 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2018 2476 2018 Size: 458 5192 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2270 3713 4310 5097 5365 1239 Size: 393 638 1443 597 787 268 1524 Fragments arranged by size: 1524 1443 787 638 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2578 2578 Size: 5650 EcoO109I rG'GnC_Cy Cuts at: 2177 2177 Size: 5650 EcoRI G'AATT_C Cuts at: 2959 3337 2959 Size: 378 5272 FspI TGC'GCA Cuts at: 4156 4156 Size: 5650 HaeII r_GCGC'y Cuts at: 1678 4797 1678 Size: 3119 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2187 2219 4291 4323 4365 Size: 32 135 32 615 32 2072 32 42 Cuts at: 4365 4397 1373 Size: 32 2626 Fragments arranged by size: 2626 2072 615 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2863 2994 3240 669 Size: 988 281 925 131 246 3079 Fragments arranged by size: 3079 988 925 281 246 131 HindIII A'AGCT_T Cuts at: 748 748 Size: 5650 HpaI GTT'AAC Cuts at: 2863 3240 2863 Size: 377 5273 KpnI G_GTAC'C Cuts at: 2975 2975 Size: 5650 MfeI C'AATT_G Cuts at: 3249 3249 Size: 5650 MluI A'CGCG_T Cuts at: 5526 5526 Size: 5650 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4764 4948 1305 Size: 3459 184 2007 Fragments arranged by size: 3459 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3828 Fragments arranged by size: 3828 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3608 3967 4126 518 Size: 648 2442 359 159 2042 Fragments arranged by size: 2442 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2880 3066 3743 4891 5136 1606 Size: 1274 186 677 1148 245 2120 Fragments arranged by size: 2120 1274 1148 677 245 186 NcoI C'CATG_G Cuts at: 513 1065 2004 2769 513 Size: 552 939 765 3394 Fragments arranged by size: 3394 939 765 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5650 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5650 NotI GC'GGCC_GC Cuts at: 3097 3097 Size: 5650 NspI r_CATG'y Cuts at: 1132 1229 2123 3008 4553 1132 Size: 97 894 885 1545 2229 Fragments arranged by size: 2229 1545 894 885 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5650 PciI A'CATG_T Cuts at: 2119 4549 2119 Size: 2430 3220 PflMI CCAn_nnn'nTGG Cuts at: 1108 2198 1108 Size: 1090 4560 PmeI GTTT'AAAC Cuts at: 2954 2954 Size: 5650 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3719 3751 5232 5264 3719 Size: 32 1481 32 4105 Fragments arranged by size: 4105 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5650 PsiI TTA'TAA Cuts at: 3220 3220 Size: 5650 PstI C_TGCA'G Cuts at: 830 2052 3002 830 Size: 1222 950 3478 Fragments arranged by size: 3478 1222 950 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5650 SacI G_AGCT'C Cuts at: 2020 2478 2020 Size: 458 5192 SalI G'TCGA_C Cuts at: 1936 2992 1936 Size: 1056 4594 SbfI CC_TGCA'GG Cuts at: 3002 3002 Size: 5650 ScaI AGT'ACT Cuts at: 3898 5610 3898 Size: 1712 3938 SfcI C'TryA_G Cuts at: 826 1045 2048 2530 2998 4133 4814 5005 826 Size: 219 1003 482 468 1135 681 191 1471 Fragments arranged by size: 1471 1135 1003 681 482 468 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5650 SmaI CCC'GGG Cuts at: 1872 2977 1872 Size: 1105 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2204 2477 2492 3754 4655 4917 Size: 197 926 261 273 15 1262 901 262 Cuts at: 4917 820 Size: 1553 Fragments arranged by size: 1553 1262 926 901 273 262 261 197 15 SnaBI TAC'GTA Cuts at: 493 3441 493 Size: 2948 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5650 SphI G_CATG'C Cuts at: 1132 3008 1132 Size: 1876 3774 SspI AAT'ATT Cuts at: 5 52 3574 5 Size: 47 3522 2081 Fragments arranged by size: 3522 2081 47 StuI AGG'CCT Cuts at: 1517 2584 2869 1517 Size: 1067 285 4298 Fragments arranged by size: 4298 1067 285 StyI C'CwwG_G Cuts at: 513 1065 2004 2279 2579 2769 2820 2927 3089 Size: 552 939 275 300 190 51 107 162 Cuts at: 3089 513 Size: 3074 Fragments arranged by size: 3074 939 552 300 275 190 162 107 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2123 2762 3677 3694 3847 4032 4477 Size: 1255 543 639 915 17 153 185 445 Cuts at: 4477 325 Size: 1498 Fragments arranged by size: 1498 1255 915 639 543 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2166 2753 3896 Size: 274 80 33 51 1443 189 587 1143 Cuts at: 3896 5608 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1143 587 274 189 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5610 5642 5610 Size: 32 5618 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5650 XbaI T'CTAG_A Cuts at: 2986 2986 Size: 5650 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5650 XmaI C'CCGG_G Cuts at: 1870 2975 1870 Size: 1105 4545 XmnI GAAnn'nnTTC Cuts at: 3779 3779 Size: 5650 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3458 276 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI