Restriction map for
pFN21AB9822
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BalI BamHI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrGI BsrI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
HpaI KpnI MaeIII MamI MfeI
MflI MluI MluNI MroI MscI
MseI MunI NaeI NdeI NgoMIV
NheI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SalI SapI SbfI SexAI
SfcI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp45I
TspRI Tth111I Van91I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcsI 364 GAATTC [GA]^AATT[CT]
AlwNI 94 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 364 GAATTC [GA]^AATT[CT]
AspHI 652 GAGCTC G[AT]GC[AT]^C
AvaII 399 GGACC G^G[AT]CC
BfaI 296 CTAG C^TAG
BsiEI 420 CGGCCG CG[GA][CT]^CG
BsiHKAI 652 GAGCTC G[AT]GC[AT]^C
BsmAI 88 GAGAC GTCTC(1/5)
BsmBI 88 GAGACG CGTCTC(1/5)
BsmFI 66 GTCCC GGGAC(10/14)
BsrBI 501 GAGCGG CCGCTC(-3/-3)
BsrDI 764 GCAATG GCAATG(2/0)
BsrFI 818 GCCGGT [GA]^CCGG[CT]
Cfr10I 818 GCCGGT [GA]^CCGG[CT]
DdeI 141 CTTAG C^T[ACGT]AG
DpnII 180 GATC ^GATC
EagI 420 CGGCCG C^GGCCG
EarI 904 GAAGAG CTCTTC(1/4)
EclXI 420 CGGCCG C^GGCCG
Eco52I 420 CGGCCG C^GGCCG
EcoRI 364 GAATTC G^AATTC
HincII 448 GTCAAC GT[CT]^[GA]AC
HindII 448 GTCAAC GT[CT]^[GA]AC
HindIII 865 AAGCTT A^AGCTT
Ksp632I 904 GAAGAG CTCTTC(1/4)
MaeI 296 CTAG C^TAG
MaeII 879 ACGT A^CGT
MboI 180 GATC ^GATC
NcoI 227 CCATGG C^CATGG
NdeII 180 GATC ^GATC
NlaIII 228 CATG CATG^
NotI 419 GCGGCCGC GC^GGCCGC
SacI 652 GAGCTC GAGCT^C
Sau3AI 180 GATC ^GATC
ScaI 389 AGTACT AGT^ACT
SfaNI 434 GCATC GCATC(5/9)
Tsp509I 365 AATT ^AATT
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# TWO CUT ENZYMES ( 17 enzymes) #
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AfaI 390 GTAC GT^AC
778 GTAC
Aor51HI 131 AGCGCT AGC^GCT
689 AGCGCT
AviII 431 TGCGCA TGC^GCA
525 TGCGCA
BglI 608 GCCCGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
629 GCCCAGGGGGC
BseRI 328 GAGGAG GAGGAG(10/8)
919 GAGGAG
BssHII 348 GCGCGC G^CGCGC
350 GCGCGC
BstXI 227 CCATGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
671 CCATCACCGTGG
Eco47III 131 AGCGCT AGC^GCT
689 AGCGCT
EcoT14I 161 CCAAGG C^C[AT][AT]GG
227 CCATGG
FspI 431 TGCGCA TGC^GCA
525 TGCGCA
MslI 228 CATGGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
672 CATCACCGTG
PvuII 286 CAGCTG CAG^CTG
310 CAGCTG
RsaI 390 GTAC GT^AC
778 GTAC
SmaI 481 CCCGGG CCC^GGG
609 CCCGGG
StyI 161 CCAAGG C^C[AT][AT]GG
227 CCATGG
XmaCI 481 CCCGGG C^CCGGG
609 CCCGGG
XmaI 481 CCCGGG C^CCGGG
609 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AvaI BanI BanII BbeI BbvI
BcnI BmyI BsaHI BsaJI BsiYI
BslI BsoBI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
KspI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NlaIV PleI SacII Sau96I ScrFI
SfoI SmlI TaqI TseI TthHB8I