(Circular) (Six-base) MAPSORT of: pFN21AB9822.seq Check: 5064 from: 1 to: 5641 pFN21AB9822 5641 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3451 278 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2984 1937 Size: 1047 4594 Acc65I G'GTAC_C Cuts at: 2962 2962 Size: 5641 AclI AA'CG_TT Cuts at: 1563 3768 4141 1563 Size: 2205 373 3063 Fragments arranged by size: 3063 2205 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3704 5088 5356 1632 Size: 2072 1384 268 1917 Fragments arranged by size: 2072 1917 1384 268 AfeI AGC'GCT Cuts at: 2138 2696 2138 Size: 558 5083 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5444 AflIII A'CryG_T Cuts at: 1203 4540 5517 1203 Size: 3337 977 1327 Fragments arranged by size: 3337 1327 977 AgeI A'CCGG_T Cuts at: 4472 4472 Size: 5641 AhdI GACnn_n'nnGTC Cuts at: 4370 4370 Size: 5641 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5641 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5641 AlwNI CAG_nnn'CTG Cuts at: 2104 3384 4956 2104 Size: 1280 1572 2789 Fragments arranged by size: 2789 1572 1280 ApaI G_GGCC'C Cuts at: 2300 2486 2636 2300 Size: 186 150 5305 Fragments arranged by size: 5305 186 150 ApaLI G'TGCA_C Cuts at: 3698 4854 3698 Size: 1156 4485 ApoI r'AATT_y Cuts at: 1493 2369 2950 3176 3328 5437 1493 Size: 876 581 226 152 2109 1697 Fragments arranged by size: 2109 1697 876 581 226 152 AseI AT'TA_AT Cuts at: 160 1032 4195 160 Size: 872 3163 1606 Fragments arranged by size: 3163 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 AvaI C'yCGr_G Cuts at: 1870 1943 2309 2486 2614 2966 1870 Size: 73 366 177 128 352 4545 Fragments arranged by size: 4545 366 352 177 128 73 BamHI G'GATC_C Cuts at: 2971 3338 2971 Size: 367 5274 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2327 2624 2669 2813 2962 Size: 325 217 627 540 297 45 144 149 Cuts at: 2962 4417 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 540 325 297 217 149 144 45 BanII G_rGCy'C Cuts at: 1391 2300 2486 2636 2661 2960 1391 Size: 909 186 150 25 299 4072 Fragments arranged by size: 4072 909 299 186 150 25 BbeI G_GCGC'C Cuts at: 2331 2628 2817 2331 Size: 297 189 5155 Fragments arranged by size: 5155 297 189 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4670 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3832 3866 1903 Size: 34 1895 34 3678 Fragments arranged by size: 3678 1895 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3533 4743 3533 Size: 1210 4431 BclI T'GATC_A Cuts at: 1548 1548 Size: 5641 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2619 2640 4252 Size: 107 122 71 631 605 947 21 1612 Cuts at: 4252 136 Size: 1525 Fragments arranged by size: 1612 1525 947 631 605 122 107 71 21 BglII A'GATC_T Cuts at: 5636 5636 Size: 5641 BlpI GC'TnA_GC Cuts at: 3058 3058 Size: 5641 Bme1580I G_kGCm'C Cuts at: 1790 2300 2486 2636 2672 3702 4858 1790 Size: 510 186 150 36 1030 1156 2573 Fragments arranged by size: 2573 1156 1030 510 186 150 36 BmgBI CAC'GTC Cuts at: 1318 2885 1318 Size: 1567 4074 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3372 4330 442 Size: 416 53 83 399 24 1955 958 1753 Fragments arranged by size: 1955 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5641 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2125 2689 2125 Size: 564 5077 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4301 1239 Size: 3062 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2165 2345 2393 3766 4631 4929 2165 Size: 180 48 1373 865 298 2877 Fragments arranged by size: 2877 1373 865 298 180 48 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4304 882 Size: 617 2805 2219 Fragments arranged by size: 2805 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3432 493 Size: 711 2228 2702 Fragments arranged by size: 2702 2228 711 BsaBI GATnn'nnATC Cuts at: 5635 5635 Size: 5641 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2328 2625 2814 3448 Size: 53 83 186 936 795 297 189 634 Cuts at: 3448 3830 4465 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 795 635 634 382 297 189 186 83 53 BsaWI w'CCGG_w Cuts at: 4074 4472 4746 4893 5304 4074 Size: 398 274 147 411 4411 Fragments arranged by size: 4411 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5416 Fragments arranged by size: 5416 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2348 2939 1186 Size: 1162 591 3888 Fragments arranged by size: 3888 1162 591 BseYI C'CCAG_C Cuts at: 2263 2293 4844 5340 2263 Size: 30 2551 496 2564 Fragments arranged by size: 2564 2551 496 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5068 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2428 3091 3852 4880 1540 Size: 275 186 427 663 761 1028 2301 Fragments arranged by size: 2301 1028 761 663 427 275 186 BsiHKAI G_wGCw'C Cuts at: 2661 3702 3787 4858 2661 Size: 1041 85 1071 3444 Fragments arranged by size: 3444 1071 1041 85 BsmI GAATG_Cn' Cuts at: 3152 3245 3152 Size: 93 5548 BsmBI CGTCTCn'nnnn_ Cuts at: 2087 2087 Size: 5641 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2300 2486 2636 2661 2672 2960 3702 Size: 399 510 186 150 25 11 288 742 Cuts at: 3702 3787 4858 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 510 399 288 186 150 85 25 11 BspEI T'CCGG_A Cuts at: 5304 5304 Size: 5641 BspHI T'CATG_A Cuts at: 3530 5260 3530 Size: 1730 3911 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2996 844 Size: 2152 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2508 3528 1394 Size: 429 685 1020 3507 Fragments arranged by size: 3507 1020 685 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2776 4136 4310 65 Size: 1183 1528 1360 174 1396 Fragments arranged by size: 1528 1396 1360 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2823 4285 4472 1700 Size: 110 1013 1462 187 2869 Fragments arranged by size: 2869 1462 1013 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5641 BssHII G'CGCG_C Cuts at: 1925 2353 2355 1925 Size: 428 2 5211 Fragments arranged by size: 5211 428 2 BssSI C'ACGA_G Cuts at: 3701 4713 3701 Size: 1012 4629 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5641 BstBI TT'CG_AA Cuts at: 4445 4445 Size: 5641 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5641 BstXI CCAn_nnnn'nTGG Cuts at: 2239 2683 2239 Size: 444 5197 BstYI r'GATC_y Cuts at: 1972 2971 3338 3723 3740 5192 5278 5290 5636 Size: 999 367 385 17 1452 86 12 346 Cuts at: 5636 1972 Size: 1977 Fragments arranged by size: 1977 1452 999 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2074 2232 2348 2458 2682 513 Size: 552 939 70 158 116 110 224 3472 Fragments arranged by size: 3472 939 552 224 158 116 110 70 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2503 487 Size: 683 56 1277 3625 Fragments arranged by size: 3625 1277 683 56 BtsI GCAGTG_nn' Cuts at: 3157 3951 3971 3157 Size: 794 20 4827 Fragments arranged by size: 4827 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5641 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5606 DraI TTT'AAA Cuts at: 2945 2945 Size: 5641 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4648 809 Size: 507 3332 1802 Fragments arranged by size: 3332 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2241 2425 2509 Size: 54 1425 50 275 18 411 184 84 Cuts at: 2509 3088 3977 5606 8 Size: 579 889 1629 43 Fragments arranged by size: 1629 1425 889 579 411 275 184 84 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2425 3088 1537 Size: 275 613 663 4090 Fragments arranged by size: 4090 663 613 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2904 3571 1353 Size: 1551 667 3423 Fragments arranged by size: 3423 1551 667 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2432 4228 4602 4748 387 Size: 1064 138 435 408 1796 374 146 1280 Fragments arranged by size: 1796 1280 1064 435 408 374 146 138 EcoICRI GAG'CTC Cuts at: 2659 2659 Size: 5641 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3704 4301 5088 5356 1239 Size: 393 2072 597 787 268 1524 Fragments arranged by size: 2072 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2297 2483 2536 2632 2297 Size: 186 53 96 5306 Fragments arranged by size: 5306 186 96 53 EcoRI G'AATT_C Cuts at: 2369 2950 3328 2369 Size: 581 378 4682 Fragments arranged by size: 4682 581 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2191 2716 2191 Size: 525 5116 FspI TGC'GCA Cuts at: 2438 2532 4147 2438 Size: 94 1615 3932 Fragments arranged by size: 3932 1615 94 HaeII r_GCGC'y Cuts at: 1678 2140 2331 2628 2698 2817 4788 1678 Size: 462 191 297 70 119 1971 2531 Fragments arranged by size: 2531 1971 462 297 191 119 70 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373 Size: 32 135 32 2710 32 42 32 2626 Fragments arranged by size: 2710 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2455 2985 3231 669 Size: 988 281 517 530 246 3079 Fragments arranged by size: 3079 988 530 517 281 246 HindIII A'AGCT_T Cuts at: 748 2870 748 Size: 2122 3519 HpaI GTT'AAC Cuts at: 3231 3231 Size: 5641 KasI G'GCGC_C Cuts at: 2327 2624 2813 2327 Size: 297 189 5155 Fragments arranged by size: 5155 297 189 KpnI G_GTAC'C Cuts at: 2966 2966 Size: 5641 MfeI C'AATT_G Cuts at: 3240 3240 Size: 5641 MluI A'CGCG_T Cuts at: 5517 5517 Size: 5641 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4755 4939 1305 Size: 3450 184 2007 Fragments arranged by size: 3450 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3819 Fragments arranged by size: 3819 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2237 2681 3599 3958 4117 518 Size: 648 1071 444 918 359 159 2042 Fragments arranged by size: 2042 1071 918 648 444 359 159 MspA1I CmG'CkG Cuts at: 1606 2022 2076 2293 2317 2350 2460 2898 3057 Size: 416 54 217 24 33 110 438 159 Cuts at: 3057 3734 4882 5127 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 438 416 245 217 159 110 54 33 24 NarI GG'CG_CC Cuts at: 2328 2625 2814 2328 Size: 297 189 5155 Fragments arranged by size: 5155 297 189 NcoI C'CATG_G Cuts at: 513 1065 2004 2232 513 Size: 552 939 228 3922 Fragments arranged by size: 3922 939 552 228 NdeI CA'TA_TG Cuts at: 387 387 Size: 5641 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5641 NotI GC'GGCC_GC Cuts at: 2425 3088 2425 Size: 663 4978 NspI r_CATG'y Cuts at: 1132 1229 2999 4544 1132 Size: 97 1770 1545 2229 Fragments arranged by size: 2229 1770 1545 97 PasI CC'CwG_GG Cuts at: 1348 2636 1348 Size: 1288 4353 PciI A'CATG_T Cuts at: 4540 4540 Size: 5641 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5641 PfoI T'CCnGG_A Cuts at: 2661 2850 2661 Size: 189 5452 PmeI GTTT'AAAC Cuts at: 2945 2945 Size: 5641 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3710 3742 5223 5255 3710 Size: 32 1481 32 4096 Fragments arranged by size: 4096 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5641 PsiI TTA'TAA Cuts at: 3211 3211 Size: 5641 PspOMI G'GGCC_C Cuts at: 2296 2482 2632 2296 Size: 186 150 5305 Fragments arranged by size: 5305 186 150 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2378 2410 2378 Size: 32 5609 PstI C_TGCA'G Cuts at: 830 2993 830 Size: 2163 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5641 PvuII CAG'CTG Cuts at: 2293 2317 2293 Size: 24 5617 SacI G_AGCT'C Cuts at: 2661 2661 Size: 5641 SacII CC_GC'GG Cuts at: 2077 2351 2461 2077 Size: 274 110 5257 Fragments arranged by size: 5257 274 110 SalI G'TCGA_C Cuts at: 1936 2983 1936 Size: 1047 4594 SbfI CC_TGCA'GG Cuts at: 2993 2993 Size: 5641 ScaI AGT'ACT Cuts at: 2396 3889 5601 2396 Size: 1493 1712 2436 Fragments arranged by size: 2436 1712 1493 SfcI C'TryA_G Cuts at: 826 1045 2989 4124 4805 4996 826 Size: 219 1944 1135 681 191 1471 Fragments arranged by size: 1944 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2329 2626 2815 2329 Size: 297 189 5155 Fragments arranged by size: 5155 297 189 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5641 SmaI CCC'GGG Cuts at: 1872 2488 2616 2968 1872 Size: 616 128 352 4545 Fragments arranged by size: 4545 616 352 128 SmlI C'TyrA_G Cuts at: 820 1017 1943 2180 2360 2408 3745 4646 4908 Size: 197 926 237 180 48 1337 901 262 Cuts at: 4908 820 Size: 1553 Fragments arranged by size: 1553 1337 926 901 262 237 197 180 48 SnaBI TAC'GTA Cuts at: 493 3432 493 Size: 2939 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5641 SphI G_CATG'C Cuts at: 1132 2999 1132 Size: 1867 3774 SspI AAT'ATT Cuts at: 5 52 3565 5 Size: 47 3513 2081 Fragments arranged by size: 3513 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5641 StyI C'CwwG_G Cuts at: 513 1065 2004 2166 2232 3080 513 Size: 552 939 162 66 848 3074 Fragments arranged by size: 3074 939 848 552 162 66 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3668 3685 3838 4023 4468 325 Size: 1255 2088 17 153 185 445 1498 Fragments arranged by size: 2088 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2394 3887 5599 Size: 274 80 33 51 1443 417 1493 1712 Cuts at: 5599 96 Size: 138 Fragments arranged by size: 1712 1493 1443 417 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5601 5633 5601 Size: 32 5609 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5641 XbaI T'CTAG_A Cuts at: 2977 2977 Size: 5641 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5641 XmaI C'CCGG_G Cuts at: 1870 2486 2614 2966 1870 Size: 616 128 352 4545 Fragments arranged by size: 4545 616 352 128 XmnI GAAnn'nnTTC Cuts at: 3770 3770 Size: 5641 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3449 276 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI