Restriction map for
pFN21AB9822
(Linear) (Six-base) MAPSORT of: KIBB9822.seq Check: 2764 from: 1 to: 936
KIBB9822.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AfeI AGC'GCT
Cuts at: 0 133 691 936
Size: 133 558 245
Fragments arranged by size:
558 245 133
AlwNI CAG_nnn'CTG
Cuts at: 0 99 936
Size: 99 837
ApaI G_GGCC'C
Cuts at: 0 295 481 631 936
Size: 295 186 150 305
Fragments arranged by size:
305 295 186 150
ApoI r'AATT_y
Cuts at: 0 364 936
Size: 364 572
AvaI C'yCGr_G
Cuts at: 0 304 481 609 936
Size: 304 177 128 327
Fragments arranged by size:
327 304 177 128
BanI G'GyrC_C
Cuts at: 0 322 619 664 808 936
Size: 322 297 45 144 128
Fragments arranged by size:
322 297 144 128 45
BanII G_rGCy'C
Cuts at: 0 295 481 631 656 936
Size: 295 186 150 25 280
Fragments arranged by size:
295 280 186 150 25
BbeI G_GCGC'C
Cuts at: 0 326 623 812 936
Size: 326 297 189 124
Fragments arranged by size:
326 297 189 124
BglI GCCn_nnn'nGGC
Cuts at: 0 614 635 936
Size: 614 21 301
Fragments arranged by size:
614 301 21
Bme1580I G_kGCm'C
Cuts at: 0 295 481 631 667 936
Size: 295 186 150 36 269
Fragments arranged by size:
295 269 186 150 36
BmgBI CAC'GTC
Cuts at: 0 880 936
Size: 880 56
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 120 684 936
Size: 120 564 252
Fragments arranged by size:
564 252 120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 340 388 936
Size: 160 180 48 548
Fragments arranged by size:
548 180 160 48
BsaHI Gr'CG_yC
Cuts at: 0 323 620 809 936
Size: 323 297 189 127
Fragments arranged by size:
323 297 189 127
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 934 936
Size: 343 591 2
Fragments arranged by size:
591 343 2
BseYI C'CCAG_C
Cuts at: 0 258 288 936
Size: 258 30 648
Fragments arranged by size:
648 258 30
BsiEI CG_ry'CG
Cuts at: 0 423 936
Size: 423 513
BsiHKAI G_wGCw'C
Cuts at: 0 656 936
Size: 656 280
BsmBI CGTCTCn'nnnn_
Cuts at: 0 82 936
Size: 82 854
Bsp1286I G_dGCh'C
Cuts at: 0 295 481 631 656 667 936
Size: 295 186 150 25 11 269
Fragments arranged by size:
295 269 186 150 25 11
BsrBI CCG'CTC
Cuts at: 0 503 936
Size: 503 433
BsrDI GCAATG_nn'
Cuts at: 0 771 936
Size: 771 165
BsrFI r'CCGG_y
Cuts at: 0 818 936
Size: 818 118
BssHII G'CGCG_C
Cuts at: 0 348 350 936
Size: 348 2 586
Fragments arranged by size:
586 348 2
BstXI CCAn_nnnn'nTGG
Cuts at: 0 234 678 936
Size: 234 444 258
Fragments arranged by size:
444 258 234
BtgI C'CryG_G
Cuts at: 0 69 227 343 453 677 936
Size: 69 158 116 110 224 259
Fragments arranged by size:
259 224 158 116 110 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 498 936
Size: 498 438
EaeI y'GGCC_r
Cuts at: 0 236 420 504 936
Size: 236 184 84 432
Fragments arranged by size:
432 236 184 84
EagI C'GGCC_G
Cuts at: 0 420 936
Size: 420 516
EarI CTCTTCn'nnn_
Cuts at: 0 899 936
Size: 899 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 427 936
Size: 19 408 509
Fragments arranged by size:
509 408 19
EcoICRI GAG'CTC
Cuts at: 0 654 936
Size: 654 282
EcoO109I rG'GnC_Cy
Cuts at: 0 292 478 531 627 936
Size: 292 186 53 96 309
Fragments arranged by size:
309 292 186 96 53
EcoRI G'AATT_C
Cuts at: 0 364 936
Size: 364 572
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 186 711 936
Size: 186 525 225
Fragments arranged by size:
525 225 186
FspI TGC'GCA
Cuts at: 0 433 527 936
Size: 433 94 409
Fragments arranged by size:
433 409 94
HaeII r_GCGC'y
Cuts at: 0 135 326 623 693 812 936
Size: 135 191 297 70 119 124
Fragments arranged by size:
297 191 135 124 119 70
HincII GTy'rAC
Cuts at: 0 450 936
Size: 450 486
HindIII A'AGCT_T
Cuts at: 0 865 936
Size: 865 71
KasI G'GCGC_C
Cuts at: 0 322 619 808 936
Size: 322 297 189 128
Fragments arranged by size:
322 297 189 128
MslI CAynn'nnrTG
Cuts at: 0 232 676 936
Size: 232 444 260
Fragments arranged by size:
444 260 232
MspA1I CmG'CkG
Cuts at: 0 17 71 288 312 345 455 893 936
Size: 17 54 217 24 33 110 438 43
Fragments arranged by size:
438 217 110 54 43 33 24 17
NarI GG'CG_CC
Cuts at: 0 323 620 809 936
Size: 323 297 189 127
Fragments arranged by size:
323 297 189 127
NcoI C'CATG_G
Cuts at: 0 227 936
Size: 227 709
NotI GC'GGCC_GC
Cuts at: 0 420 936
Size: 420 516
PasI CC'CwG_GG
Cuts at: 0 631 936
Size: 631 305
PfoI T'CCnGG_A
Cuts at: 0 656 845 936
Size: 656 189 91
Fragments arranged by size:
656 189 91
PspOMI G'GGCC_C
Cuts at: 0 291 477 627 936
Size: 291 186 150 309
Fragments arranged by size:
309 291 186 150
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 373 405 936
Size: 373 32 531
Fragments arranged by size:
531 373 32
PvuII CAG'CTG
Cuts at: 0 288 312 936
Size: 288 24 624
Fragments arranged by size:
624 288 24
SacI G_AGCT'C
Cuts at: 0 656 936
Size: 656 280
SacII CC_GC'GG
Cuts at: 0 72 346 456 936
Size: 72 274 110 480
Fragments arranged by size:
480 274 110 72
ScaI AGT'ACT
Cuts at: 0 391 936
Size: 391 545
SfoI GGC'GCC
Cuts at: 0 324 621 810 936
Size: 324 297 189 126
Fragments arranged by size:
324 297 189 126
SmaI CCC'GGG
Cuts at: 0 483 611 936
Size: 483 128 325
Fragments arranged by size:
483 325 128
SmlI C'TyrA_G
Cuts at: 0 175 355 403 936
Size: 175 180 48 533
Fragments arranged by size:
533 180 175 48
StyI C'CwwG_G
Cuts at: 0 161 227 936
Size: 161 66 709
Fragments arranged by size:
709 161 66
TatI w'GTAC_w
Cuts at: 0 389 936
Size: 389 547
XmaI C'CCGG_G
Cuts at: 0 481 609 936
Size: 481 128 327
Fragments arranged by size:
481 327 128
Enzymes that do cut:
AfeI AlwNI ApaI ApoI AvaI BanI BanII BbeI
BglI Bme1580I BmgBI BplI BpuEI BsaHI BseRI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BsrFI BssHII
BstXI BtgI BtgZI EaeI EagI EarI EciI EcoICRI
EcoO109I EcoRI FalI FspI HaeII HincII HindIII KasI
MslI MspA1I NarI NcoI NotI PasI PfoI PspOMI
PsrI PvuII SacI SacII ScaI SfoI SmaI SmlI
StyI TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmrI BmtI BpmI Bpu10I
BsaI BsaAI BsaBI BsaWI BsaXI BsgI BsiWI BsmI
BspEI BspHI BspMI BsrGI BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII
DrdI Eco57MI EcoNI EcoRV FseI FspAI Hin4I HpaI
KpnI MfeI MluI MmeI MscI NaeI NdeI NgoMIV
NheI NruI NsiI NspI PacI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
RsrII SalI SanDI SapI SbfI SexAI SfcI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmnI
ZraI