(Circular) (Six-base) MAPSORT of: pFN21AB9820.seq Check: 4195 from: 1 to: 5638 pFN21AB9820 5638 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3448 278 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2981 1937 Size: 1044 4594 Acc65I G'GTAC_C Cuts at: 2959 2959 Size: 5638 AclI AA'CG_TT Cuts at: 1563 3765 4138 1563 Size: 2202 373 3063 Fragments arranged by size: 3063 2202 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3701 5085 5353 1632 Size: 2069 1384 268 1917 Fragments arranged by size: 2069 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2774 820 Size: 197 1757 3684 Fragments arranged by size: 3684 1757 197 AflIII A'CryG_T Cuts at: 1203 4537 5514 1203 Size: 3334 977 1327 Fragments arranged by size: 3334 1327 977 AgeI A'CCGG_T Cuts at: 4469 4469 Size: 5638 AhdI GACnn_n'nnGTC Cuts at: 2359 4367 2359 Size: 2008 3630 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5638 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2157 1184 Size: 973 4665 AlwNI CAG_nnn'CTG Cuts at: 3381 4953 3381 Size: 1572 4066 ApaLI G'TGCA_C Cuts at: 3695 4851 3695 Size: 1156 4482 ApoI r'AATT_y Cuts at: 1493 2030 2947 3173 3325 5434 1493 Size: 537 917 226 152 2109 1697 Fragments arranged by size: 2109 1697 917 537 226 152 AseI AT'TA_AT Cuts at: 160 1032 4192 160 Size: 872 3160 1606 Fragments arranged by size: 3160 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 AvaI C'yCGr_G Cuts at: 1870 1943 2963 1870 Size: 73 1020 4545 Fragments arranged by size: 4545 1020 73 BamHI G'GATC_C Cuts at: 2968 3335 2968 Size: 367 5271 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2761 2832 2959 4414 618 Size: 325 217 627 974 71 127 1455 1842 Fragments arranged by size: 1842 1455 974 627 325 217 127 71 BanII G_rGCy'C Cuts at: 1391 2466 2957 1391 Size: 1075 491 4072 Fragments arranged by size: 4072 1075 491 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4667 BbvCI CC'TCA_GC Cuts at: 2337 2481 2337 Size: 144 5494 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3829 3863 1903 Size: 34 1892 34 3678 Fragments arranged by size: 3678 1892 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2450 3530 4740 2450 Size: 1080 1210 3348 Fragments arranged by size: 3348 1210 1080 BclI T'GATC_A Cuts at: 1548 1548 Size: 5638 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2274 4249 136 Size: 107 122 71 631 605 602 1975 1525 Fragments arranged by size: 1975 1525 631 605 602 122 107 71 BglII A'GATC_T Cuts at: 2655 5633 2655 Size: 2978 2660 BlpI GC'TnA_GC Cuts at: 2130 3055 2130 Size: 925 4713 Bme1580I G_kGCm'C Cuts at: 1790 2270 2835 3699 4855 1790 Size: 480 565 864 1156 2573 Fragments arranged by size: 2573 1156 864 565 480 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5638 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2083 2193 2292 Size: 416 53 83 399 24 666 110 99 Cuts at: 2292 2445 3369 4327 442 Size: 153 924 958 1753 Fragments arranged by size: 1753 958 924 666 416 399 153 110 99 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5638 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2392 2489 4298 1239 Size: 1153 97 1809 2579 Fragments arranged by size: 2579 1809 1153 97 Bpu10I CC'TnA_GC Cuts at: 2337 2481 2863 2337 Size: 144 382 5112 Fragments arranged by size: 5112 382 144 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3763 4628 4926 3763 Size: 865 298 4475 Fragments arranged by size: 4475 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2188 4301 882 Size: 617 689 2113 2219 Fragments arranged by size: 2219 2113 689 617 BsaAI yAC'GTr Cuts at: 493 1204 3429 493 Size: 711 2225 2702 Fragments arranged by size: 2702 2225 711 BsaBI GATnn'nnATC Cuts at: 5632 5632 Size: 5638 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3445 3827 4462 275 Size: 53 83 186 936 1912 382 635 1451 Fragments arranged by size: 1912 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2701 4071 4469 4743 4890 5301 2701 Size: 1370 398 274 147 411 3038 Fragments arranged by size: 3038 1370 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2185 2215 2483 2513 1178 Size: 30 165 30 782 30 268 30 4303 Fragments arranged by size: 4303 782 268 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2206 2489 2864 1186 Size: 1020 283 375 3960 Fragments arranged by size: 3960 1020 375 283 BseYI C'CCAG_C Cuts at: 4841 5337 4841 Size: 496 5142 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2669 1163 Size: 573 933 4132 Fragments arranged by size: 4132 933 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3088 3849 4877 1540 Size: 275 186 1087 761 1028 2301 Fragments arranged by size: 2301 1087 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2123 2466 2720 3699 3784 4855 2123 Size: 343 254 979 85 1071 2906 Fragments arranged by size: 2906 1071 979 343 254 85 BsmI GAATG_Cn' Cuts at: 3149 3242 3149 Size: 93 5545 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2123 2270 2466 2720 2835 2957 3699 Size: 399 333 147 196 254 115 122 742 Cuts at: 3699 3784 4855 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 333 254 196 147 122 115 85 BspEI T'CCGG_A Cuts at: 2701 5301 2701 Size: 2600 3038 BspHI T'CATG_A Cuts at: 3527 5257 3527 Size: 1730 3908 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2993 844 Size: 2149 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3525 1394 Size: 429 1702 3507 Fragments arranged by size: 3507 1702 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4133 4307 65 Size: 1183 2885 174 1396 Fragments arranged by size: 2885 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4282 4469 1700 Size: 110 2472 187 2869 Fragments arranged by size: 2869 2472 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5638 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5638 BssSI C'ACGA_G Cuts at: 2856 3698 4710 2856 Size: 842 1012 3784 Fragments arranged by size: 3784 1012 842 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5638 BstBI TT'CG_AA Cuts at: 4442 4442 Size: 5638 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5638 BstYI r'GATC_y Cuts at: 1972 2311 2655 2968 3335 3720 3737 5189 5275 Size: 339 344 313 367 385 17 1452 86 Cuts at: 5275 5287 5633 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 344 339 313 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2047 513 Size: 552 939 43 4104 Fragments arranged by size: 4104 939 552 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4899 Fragments arranged by size: 4899 683 56 BtsI GCAGTG_nn' Cuts at: 3154 3948 3968 3154 Size: 794 20 4824 Fragments arranged by size: 4824 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5638 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5603 DraI TTT'AAA Cuts at: 2942 2942 Size: 5638 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4645 809 Size: 507 3329 1802 Fragments arranged by size: 3329 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2050 3085 3974 Size: 54 1425 50 275 18 220 1035 889 Cuts at: 3974 5603 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1035 889 275 220 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3085 1537 Size: 275 1273 4090 Fragments arranged by size: 4090 1273 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2183 2466 2708 3568 1353 Size: 830 283 242 860 3423 Fragments arranged by size: 3423 860 830 283 242 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4225 4599 4745 387 Size: 1064 138 2636 374 146 1280 Fragments arranged by size: 2636 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2464 2464 Size: 5638 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2392 2489 3701 4298 5085 5353 1239 Size: 393 760 97 1212 597 787 268 1524 Fragments arranged by size: 1524 1212 787 760 597 393 268 97 EcoNI CCTnn'n_nnAGG Cuts at: 2575 2575 Size: 5638 EcoO109I rG'GnC_Cy Cuts at: 2056 2287 2580 2056 Size: 231 293 5114 Fragments arranged by size: 5114 293 231 EcoRI G'AATT_C Cuts at: 2947 3325 2947 Size: 378 5260 FspI TGC'GCA Cuts at: 4144 4144 Size: 5638 HaeII r_GCGC'y Cuts at: 1678 4785 1678 Size: 3107 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2105 2137 2185 2217 2345 Size: 32 135 32 533 32 48 32 128 Cuts at: 2345 2377 2483 2515 4279 4311 4353 4385 1373 Size: 32 106 32 1764 32 42 32 2626 Fragments arranged by size: 2626 1764 533 135 128 106 48 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2919 2982 3228 669 Size: 988 281 981 63 246 3079 Fragments arranged by size: 3079 988 981 281 246 63 HindIII A'AGCT_T Cuts at: 748 748 Size: 5638 HpaI GTT'AAC Cuts at: 3228 3228 Size: 5638 KpnI G_GTAC'C Cuts at: 2963 2963 Size: 5638 MfeI C'AATT_G Cuts at: 2590 3237 2590 Size: 647 4991 MluI A'CGCG_T Cuts at: 5514 5514 Size: 5638 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2137 4752 4936 1305 Size: 832 2615 184 2007 Fragments arranged by size: 2615 2007 832 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2052 10 Size: 54 1425 343 220 3596 Fragments arranged by size: 3596 1425 343 220 54 MslI CAynn'nnrTG Cuts at: 518 1166 3596 3955 4114 518 Size: 648 2430 359 159 2042 Fragments arranged by size: 2430 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3054 3731 4879 5124 1606 Size: 1448 677 1148 245 2120 Fragments arranged by size: 2120 1448 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2047 513 Size: 552 939 43 4104 Fragments arranged by size: 4104 939 552 43 NdeI CA'TA_TG Cuts at: 387 387 Size: 5638 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5638 NotI GC'GGCC_GC Cuts at: 3085 3085 Size: 5638 NspI r_CATG'y Cuts at: 1132 1229 2996 4541 1132 Size: 97 1767 1545 2229 Fragments arranged by size: 2229 1767 1545 97 PasI CC'CwG_GG Cuts at: 1348 2060 1348 Size: 712 4926 PciI A'CATG_T Cuts at: 4537 4537 Size: 5638 PflMI CCAn_nnn'nTGG Cuts at: 1108 2238 1108 Size: 1130 4508 PfoI T'CCnGG_A Cuts at: 2742 2742 Size: 5638 PmeI GTTT'AAAC Cuts at: 2942 2942 Size: 5638 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3707 3739 5220 5252 3707 Size: 32 1481 32 4093 Fragments arranged by size: 4093 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2056 2056 Size: 5638 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5638 PsiI TTA'TAA Cuts at: 3208 3208 Size: 5638 PstI C_TGCA'G Cuts at: 830 2990 830 Size: 2160 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5638 SacI G_AGCT'C Cuts at: 2466 2466 Size: 5638 SalI G'TCGA_C Cuts at: 1936 2980 1936 Size: 1044 4594 SapI GCTCTTCn'nnn_ Cuts at: 2708 2708 Size: 5638 SbfI CC_TGCA'GG Cuts at: 2990 2990 Size: 5638 ScaI AGT'ACT Cuts at: 3886 5598 3886 Size: 1712 3926 SfcI C'TryA_G Cuts at: 826 1045 2986 4121 4802 4993 826 Size: 219 1941 1135 681 191 1471 Fragments arranged by size: 1941 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5638 SmaI CCC'GGG Cuts at: 1872 2965 1872 Size: 1093 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2774 3742 4643 4905 820 Size: 197 926 831 968 901 262 1553 Fragments arranged by size: 1553 968 926 901 831 262 197 SnaBI TAC'GTA Cuts at: 493 3429 493 Size: 2936 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5638 SphI G_CATG'C Cuts at: 1132 2996 1132 Size: 1864 3774 SspI AAT'ATT Cuts at: 5 52 3562 5 Size: 47 3510 2081 Fragments arranged by size: 3510 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5638 StyI C'CwwG_G Cuts at: 513 1065 2004 2047 2272 3077 513 Size: 552 939 43 225 805 3074 Fragments arranged by size: 3074 939 805 552 225 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2236 3665 3682 3835 4020 4465 325 Size: 1255 656 1429 17 153 185 445 1498 Fragments arranged by size: 1498 1429 1255 656 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3884 5596 96 Size: 274 80 33 51 1443 1907 1712 138 Fragments arranged by size: 1907 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5598 5630 5598 Size: 32 5606 Tth111I GACn'n_nGTC Cuts at: 1316 2199 1316 Size: 883 4755 XbaI T'CTAG_A Cuts at: 2974 2974 Size: 5638 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5638 XmaI C'CCGG_G Cuts at: 1870 2963 1870 Size: 1093 4545 XmnI GAAnn'nnTTC Cuts at: 3767 3767 Size: 5638 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3446 276 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI