(Circular) (Six-base) MAPSORT of: pFN21AB9819.seq Check: 8819 from: 1 to: 5635 pFN21AB9819 5635 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2445 2829 2445 Size: 384 5251 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3445 278 Size: 53 83 186 936 1909 2468 Fragments arranged by size: 2468 1909 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2978 1937 Size: 1041 4594 Acc65I G'GTAC_C Cuts at: 2956 2956 Size: 5635 AclI AA'CG_TT Cuts at: 1563 3762 4135 1563 Size: 2199 373 3063 Fragments arranged by size: 3063 2199 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3698 5082 5350 1632 Size: 2066 1384 268 1917 Fragments arranged by size: 2066 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5438 AflIII A'CryG_T Cuts at: 1203 2932 4534 5511 1203 Size: 1729 1602 977 1327 Fragments arranged by size: 1729 1602 1327 977 AgeI A'CCGG_T Cuts at: 4466 4466 Size: 5635 AhdI GACnn_n'nnGTC Cuts at: 4364 4364 Size: 5635 AleI CACnn'nnGTG Cuts at: 1166 2571 1166 Size: 1405 4230 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2589 1184 Size: 1405 4230 AlwNI CAG_nnn'CTG Cuts at: 3378 4950 3378 Size: 1572 4063 ApaI G_GGCC'C Cuts at: 2094 2094 Size: 5635 ApaLI G'TGCA_C Cuts at: 2432 3692 4848 2432 Size: 1260 1156 3219 Fragments arranged by size: 3219 1260 1156 ApoI r'AATT_y Cuts at: 1493 2944 3170 3322 5431 1493 Size: 1451 226 152 2109 1697 Fragments arranged by size: 2109 1697 1451 226 152 AseI AT'TA_AT Cuts at: 160 1032 4189 160 Size: 872 3157 1606 Fragments arranged by size: 3157 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5635 AvaI C'yCGr_G Cuts at: 1870 1943 2330 2729 2879 2960 1870 Size: 73 387 399 150 81 4545 Fragments arranged by size: 4545 399 387 150 81 73 BamHI G'GATC_C Cuts at: 2965 3332 2965 Size: 367 5268 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2573 2625 2883 2956 4411 Size: 325 217 627 786 52 258 73 1455 Cuts at: 4411 618 Size: 1842 Fragments arranged by size: 1842 1455 786 627 325 258 217 73 52 BanII G_rGCy'C Cuts at: 1391 2094 2306 2811 2954 1391 Size: 703 212 505 143 4072 Fragments arranged by size: 4072 703 505 212 143 BbeI G_GCGC'C Cuts at: 2887 2887 Size: 5635 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4664 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3826 3860 1903 Size: 34 1889 34 3678 Fragments arranged by size: 3678 1889 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3527 4737 3527 Size: 1210 4425 BclI T'GATC_A Cuts at: 1548 2754 1548 Size: 1206 4429 BfrBI ATG'CAT Cuts at: 2277 2277 Size: 5635 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2161 2493 2634 Size: 107 122 71 631 605 489 332 141 Cuts at: 2634 2689 2881 4246 136 Size: 55 192 1365 1525 Fragments arranged by size: 1525 1365 631 605 489 332 192 141 122 107 71 55 BglII A'GATC_T Cuts at: 5630 5630 Size: 5635 BlpI GC'TnA_GC Cuts at: 2531 2591 3052 2531 Size: 60 461 5114 Fragments arranged by size: 5114 461 60 Bme1580I G_kGCm'C Cuts at: 1790 2094 2436 3696 4852 1790 Size: 304 342 1260 1156 2573 Fragments arranged by size: 2573 1260 1156 342 304 BmgBI CAC'GTC Cuts at: 1318 2137 1318 Size: 819 4816 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2410 3366 4324 Size: 416 53 83 399 24 993 956 958 Cuts at: 4324 442 Size: 1753 Fragments arranged by size: 1753 993 958 956 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5635 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2468 2521 4295 1239 Size: 1229 53 1774 2579 Fragments arranged by size: 2579 1774 1229 53 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3760 4625 4923 3760 Size: 865 298 4472 Fragments arranged by size: 4472 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2110 4298 882 Size: 617 611 2188 2219 Fragments arranged by size: 2219 2188 617 611 BsaAI yAC'GTr Cuts at: 493 1204 2569 3426 493 Size: 711 1365 857 2702 Fragments arranged by size: 2702 1365 857 711 BsaBI GATnn'nnATC Cuts at: 5629 5629 Size: 5635 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2475 2884 3442 3824 Size: 53 83 186 936 942 409 558 382 Cuts at: 3824 4459 275 Size: 635 1451 Fragments arranged by size: 1451 942 936 635 558 409 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4068 4466 4740 4887 5298 4068 Size: 398 274 147 411 4405 Fragments arranged by size: 4405 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2102 2132 2363 2393 2860 Size: 30 165 30 699 30 231 30 467 Cuts at: 2860 2890 2910 2940 1178 Size: 30 20 30 3873 Fragments arranged by size: 3873 699 467 231 165 30 30 30 30 30 30 20 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2855 2921 2924 1186 Size: 1669 66 3 3897 Fragments arranged by size: 3897 1669 66 3 BseYI C'CCAG_C Cuts at: 2211 2897 4838 5334 2211 Size: 686 1941 496 2512 Fragments arranged by size: 2512 1941 686 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2222 2423 2807 1163 Size: 573 486 201 384 3991 Fragments arranged by size: 3991 573 486 384 201 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2061 2497 2796 3085 3846 4874 Size: 275 186 60 436 299 289 761 1028 Cuts at: 4874 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 436 299 289 275 186 60 BsiHKAI G_wGCw'C Cuts at: 2436 2811 3696 3781 4852 2436 Size: 375 885 85 1071 3219 Fragments arranged by size: 3219 1071 885 375 85 BsmI GAATG_Cn' Cuts at: 2255 2279 3146 3239 2255 Size: 24 867 93 4651 Fragments arranged by size: 4651 867 93 24 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2306 2436 2811 2954 3696 3781 Size: 399 304 212 130 375 143 742 85 Cuts at: 3781 4852 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 375 304 212 143 130 85 BspEI T'CCGG_A Cuts at: 5298 5298 Size: 5635 BspHI T'CATG_A Cuts at: 3524 5254 3524 Size: 1730 3905 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2346 2445 2829 2990 844 Size: 1502 99 384 161 3489 Fragments arranged by size: 3489 1502 384 161 99 BsrBI CCG'CTC Cuts at: 1394 1823 3522 1394 Size: 429 1699 3507 Fragments arranged by size: 3507 1699 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4130 4304 65 Size: 1183 2882 174 1396 Fragments arranged by size: 2882 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2442 2688 4279 4466 1700 Size: 110 632 246 1591 187 2869 Fragments arranged by size: 2869 1591 632 246 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5635 BssHII G'CGCG_C Cuts at: 1925 2788 1925 Size: 863 4772 BssSI C'ACGA_G Cuts at: 2240 3695 4707 2240 Size: 1455 1012 3168 Fragments arranged by size: 3168 1455 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5635 BstBI TT'CG_AA Cuts at: 4439 4439 Size: 5635 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5635 BstXI CCAn_nnnn'nTGG Cuts at: 2545 2545 Size: 5635 BstYI r'GATC_y Cuts at: 1972 2965 3332 3717 3734 5186 5272 5284 5630 Size: 993 367 385 17 1452 86 12 346 Cuts at: 5630 1972 Size: 1977 Fragments arranged by size: 1977 1452 993 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2157 2157 Size: 5635 BtgI C'CryG_G Cuts at: 513 1065 2004 2055 2231 2544 513 Size: 552 939 51 176 313 3604 Fragments arranged by size: 3604 939 552 313 176 51 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4896 Fragments arranged by size: 4896 683 56 BtsI GCAGTG_nn' Cuts at: 2931 3151 3945 3965 2931 Size: 220 794 20 4601 Fragments arranged by size: 4601 794 220 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5635 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5600 DraI TTT'AAA Cuts at: 2939 2939 Size: 5635 DraIII CAC_nnn'GTG Cuts at: 2245 2245 Size: 5635 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4642 809 Size: 507 3326 1802 Fragments arranged by size: 3326 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2494 2793 Size: 54 1425 50 275 18 228 436 299 Cuts at: 2793 3082 3971 5600 8 Size: 289 889 1629 43 Fragments arranged by size: 1629 1425 889 436 299 289 275 228 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2058 2494 2793 3082 1537 Size: 275 246 436 299 289 4090 Fragments arranged by size: 4090 436 299 289 275 246 EarI CTCTTCn'nnn_ Cuts at: 1353 2282 3565 1353 Size: 929 1283 3423 Fragments arranged by size: 3423 1283 929 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2846 4222 4596 4742 387 Size: 1064 138 1257 1376 374 146 1280 Fragments arranged by size: 1376 1280 1257 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2809 2809 Size: 5635 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2468 2521 3698 4295 5082 5350 1239 Size: 393 836 53 1177 597 787 268 1524 Fragments arranged by size: 1524 1177 836 787 597 393 268 53 EcoO109I rG'GnC_Cy Cuts at: 2091 2749 2091 Size: 658 4977 EcoRI G'AATT_C Cuts at: 2944 3322 2944 Size: 378 5257 FspI TGC'GCA Cuts at: 2552 2669 4141 2552 Size: 117 1472 4046 Fragments arranged by size: 4046 1472 117 HaeII r_GCGC'y Cuts at: 1678 2315 2544 2887 4782 1678 Size: 637 229 343 1895 2531 Fragments arranged by size: 2531 1895 637 343 229 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2363 2395 4276 4308 4350 Size: 32 135 32 791 32 1881 32 42 Cuts at: 4350 4382 1373 Size: 32 2626 Fragments arranged by size: 2626 1881 791 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2506 2979 3225 669 Size: 988 281 568 473 246 3079 Fragments arranged by size: 3079 988 568 473 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5635 HpaI GTT'AAC Cuts at: 3225 3225 Size: 5635 KasI G'GCGC_C Cuts at: 2883 2883 Size: 5635 KpnI G_GTAC'C Cuts at: 2960 2960 Size: 5635 MfeI C'AATT_G Cuts at: 3234 3234 Size: 5635 MluI A'CGCG_T Cuts at: 5511 5511 Size: 5635 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4749 4933 1305 Size: 3444 184 2007 Fragments arranged by size: 3444 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3813 Fragments arranged by size: 3813 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2571 3593 3952 4111 518 Size: 648 1405 1022 359 159 2042 Fragments arranged by size: 2042 1405 1022 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2233 2704 3051 3728 4876 5121 1606 Size: 627 471 347 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 627 471 347 245 NarI GG'CG_CC Cuts at: 2884 2884 Size: 5635 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4144 Fragments arranged by size: 4144 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5635 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5635 NotI GC'GGCC_GC Cuts at: 2494 3082 2494 Size: 588 5047 NsiI A_TGCA'T Cuts at: 2279 2279 Size: 5635 NspI r_CATG'y Cuts at: 1132 1229 2993 4538 1132 Size: 97 1764 1545 2229 Fragments arranged by size: 2229 1764 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5635 PciI A'CATG_T Cuts at: 4534 4534 Size: 5635 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5635 PmeI GTTT'AAAC Cuts at: 2939 2939 Size: 5635 PmlI CAC'GTG Cuts at: 2569 2569 Size: 5635 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3704 3736 5217 5249 3704 Size: 32 1481 32 4090 Fragments arranged by size: 4090 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2749 2749 Size: 5635 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5635 PsiI TTA'TAA Cuts at: 3205 3205 Size: 5635 PspOMI G'GGCC_C Cuts at: 2090 2090 Size: 5635 PspXI vC'TCGA_Gb Cuts at: 2729 2729 Size: 5635 PstI C_TGCA'G Cuts at: 830 2697 2805 2987 830 Size: 1867 108 182 3478 Fragments arranged by size: 3478 1867 182 108 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5635 PvuII CAG'CTG Cuts at: 2704 2704 Size: 5635 SacI G_AGCT'C Cuts at: 2811 2811 Size: 5635 SacII CC_GC'GG Cuts at: 2234 2234 Size: 5635 SalI G'TCGA_C Cuts at: 1936 2977 1936 Size: 1041 4594 SapI GCTCTTCn'nnn_ Cuts at: 2282 2282 Size: 5635 SbfI CC_TGCA'GG Cuts at: 2987 2987 Size: 5635 ScaI AGT'ACT Cuts at: 3883 5595 3883 Size: 1712 3923 SexAI A'CCwGG_T Cuts at: 2013 2013 Size: 5635 SfcI C'TryA_G Cuts at: 826 1045 2693 2801 2983 4118 4799 4990 826 Size: 219 1648 108 182 1135 681 191 1471 Fragments arranged by size: 1648 1471 1135 681 219 191 182 108 SfiI GGCCn_nnn'nGGCC Cuts at: 2493 2493 Size: 5635 SfoI GGC'GCC Cuts at: 2885 2885 Size: 5635 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5635 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5635 SmaI CCC'GGG Cuts at: 1872 2881 2962 1872 Size: 1009 81 4545 Fragments arranged by size: 4545 1009 81 SmlI C'TyrA_G Cuts at: 820 1017 1943 2729 3739 4640 4902 820 Size: 197 926 786 1010 901 262 1553 Fragments arranged by size: 1553 1010 926 901 786 262 197 SnaBI TAC'GTA Cuts at: 493 3426 493 Size: 2933 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5635 SphI G_CATG'C Cuts at: 1132 2993 1132 Size: 1861 3774 SspI AAT'ATT Cuts at: 5 52 3559 5 Size: 47 3507 2081 Fragments arranged by size: 3507 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5635 StyI C'CwwG_G Cuts at: 513 1065 2004 2577 3074 513 Size: 552 939 573 497 3074 Fragments arranged by size: 3074 939 573 552 497 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2561 3662 3679 3832 4017 4462 325 Size: 1255 981 1101 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1101 981 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3881 5593 96 Size: 274 80 33 51 1443 1904 1712 138 Fragments arranged by size: 1904 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2910 2942 5595 5627 2910 Size: 32 2653 32 2918 Fragments arranged by size: 2918 2653 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5635 XbaI T'CTAG_A Cuts at: 2743 2971 2743 Size: 228 5407 XhoI C'TCGA_G Cuts at: 1943 2729 1943 Size: 786 4849 XmaI C'CCGG_G Cuts at: 1870 2879 2960 1870 Size: 1009 81 4545 Fragments arranged by size: 4545 1009 81 XmnI GAAnn'nnTTC Cuts at: 2291 3764 2291 Size: 1473 4162 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3443 276 Size: 53 83 186 936 1909 2468 Fragments arranged by size: 2468 1909 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PsrI RsrII SanDI SrfI SwaI XcmI