(Circular) (Six-base) MAPSORT of: pFN21AB9818.seq Check: 5600 from: 1 to: 5632 pFN21AB9818 5632 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2531 2752 2531 Size: 221 5411 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3442 278 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2975 1937 Size: 1038 4594 Acc65I G'GTAC_C Cuts at: 2953 2953 Size: 5632 AclI AA'CG_TT Cuts at: 1563 3759 4132 1563 Size: 2196 373 3063 Fragments arranged by size: 3063 2196 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3695 5079 5347 1632 Size: 2063 1384 268 1917 Fragments arranged by size: 2063 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5435 AflIII A'CryG_T Cuts at: 1203 4531 5508 1203 Size: 3328 977 1327 Fragments arranged by size: 3328 1327 977 AgeI A'CCGG_T Cuts at: 4463 4463 Size: 5632 AhdI GACnn_n'nnGTC Cuts at: 4361 4361 Size: 5632 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5632 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5632 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2279 2311 2279 Size: 32 5600 AlwNI CAG_nnn'CTG Cuts at: 3375 4947 3375 Size: 1572 4060 ApaI G_GGCC'C Cuts at: 2815 2815 Size: 5632 ApaLI G'TGCA_C Cuts at: 3689 4845 3689 Size: 1156 4476 ApoI r'AATT_y Cuts at: 1493 2941 3167 3319 5428 1493 Size: 1448 226 152 2109 1697 Fragments arranged by size: 2109 1697 1448 226 152 AseI AT'TA_AT Cuts at: 160 1032 4186 160 Size: 872 3154 1606 Fragments arranged by size: 3154 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 AvaI C'yCGr_G Cuts at: 1870 1943 2957 1870 Size: 73 1014 4545 Fragments arranged by size: 4545 1014 73 AvrII C'CTAG_G Cuts at: 2527 2527 Size: 5632 BamHI G'GATC_C Cuts at: 2962 3329 2962 Size: 367 5265 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2126 2140 2764 2953 4408 Size: 325 217 627 339 14 624 189 1455 Cuts at: 4408 618 Size: 1842 Fragments arranged by size: 1842 1455 627 624 339 325 217 189 14 BanII G_rGCy'C Cuts at: 1391 2709 2815 2951 1391 Size: 1318 106 136 4072 Fragments arranged by size: 4072 1318 136 106 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4661 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3823 3857 1903 Size: 34 1886 34 3678 Fragments arranged by size: 3678 1886 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2291 3524 4734 2291 Size: 1233 1210 3189 Fragments arranged by size: 3189 1233 1210 BclI T'GATC_A Cuts at: 1548 2853 1548 Size: 1305 4327 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2054 4243 136 Size: 107 122 71 631 605 382 2189 1525 Fragments arranged by size: 2189 1525 631 605 382 122 107 71 BglII A'GATC_T Cuts at: 2430 5627 2430 Size: 3197 2435 BlpI GC'TnA_GC Cuts at: 3049 3049 Size: 5632 Bme1580I G_kGCm'C Cuts at: 1790 2129 2145 2815 3693 4849 1790 Size: 339 16 670 878 1156 2573 Fragments arranged by size: 2573 1156 878 670 339 16 BmgBI CAC'GTC Cuts at: 1318 2479 1318 Size: 1161 4471 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2226 3363 4321 Size: 416 53 83 399 24 809 1137 958 Cuts at: 4321 442 Size: 1753 Fragments arranged by size: 1753 1137 958 809 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5632 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2153 2306 2411 2771 4292 1239 Size: 914 153 105 360 1521 2579 Fragments arranged by size: 2579 1521 914 360 153 105 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2033 2072 2864 3757 4622 4920 2033 Size: 39 792 893 865 298 2745 Fragments arranged by size: 2745 893 865 792 298 39 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2537 4295 882 Size: 617 1038 1758 2219 Fragments arranged by size: 2219 1758 1038 617 BsaAI yAC'GTr Cuts at: 493 1204 3423 493 Size: 711 2219 2702 Fragments arranged by size: 2702 2219 711 BsaBI GATnn'nnATC Cuts at: 5626 5626 Size: 5632 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3439 3821 4456 275 Size: 53 83 186 936 1906 382 635 1451 Fragments arranged by size: 1906 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4065 4463 4737 4884 5295 4065 Size: 398 274 147 411 4402 Fragments arranged by size: 4402 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2149 2179 2279 2309 1178 Size: 30 165 30 746 30 100 30 4501 Fragments arranged by size: 4501 746 165 100 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2035 2092 2095 2098 2103 2150 2273 2336 Size: 849 57 3 3 5 47 123 63 Cuts at: 2336 2651 2666 2930 1186 Size: 315 15 264 3888 Fragments arranged by size: 3888 849 315 264 123 63 57 47 15 5 3 3 BseYI C'CCAG_C Cuts at: 2150 2551 4835 5331 2150 Size: 401 2284 496 2451 Fragments arranged by size: 2451 2284 496 401 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2012 2108 2756 1163 Size: 573 276 96 648 4039 Fragments arranged by size: 4039 648 573 276 96 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3082 3843 4871 1540 Size: 275 186 1081 761 1028 2301 Fragments arranged by size: 2301 1081 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2691 3693 3778 4849 2691 Size: 1002 85 1071 3474 Fragments arranged by size: 3474 1071 1002 85 BsmI GAATG_Cn' Cuts at: 3143 3236 3143 Size: 93 5539 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2129 2145 2691 2709 2815 2951 3693 Size: 399 339 16 546 18 106 136 742 Cuts at: 3693 3778 4849 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 546 399 339 136 106 85 18 16 BspEI T'CCGG_A Cuts at: 5295 5295 Size: 5632 BspHI T'CATG_A Cuts at: 2604 3521 5251 2604 Size: 917 1730 2985 Fragments arranged by size: 2985 1730 917 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2531 2752 2987 844 Size: 1687 221 235 3489 Fragments arranged by size: 3489 1687 235 221 BsrBI CCG'CTC Cuts at: 1394 1823 3519 1394 Size: 429 1696 3507 Fragments arranged by size: 3507 1696 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2619 4127 4301 65 Size: 1183 1371 1508 174 1396 Fragments arranged by size: 1508 1396 1371 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2498 2757 4276 4463 1700 Size: 110 688 259 1519 187 2869 Fragments arranged by size: 2869 1519 688 259 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5632 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5632 BssSI C'ACGA_G Cuts at: 3692 4704 3692 Size: 1012 4620 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5632 BstBI TT'CG_AA Cuts at: 4436 4436 Size: 5632 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5632 BstYI r'GATC_y Cuts at: 1972 2161 2430 2962 3329 3714 3731 5183 5269 Size: 189 269 532 367 385 17 1452 86 Cuts at: 5269 5281 5627 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 532 385 367 346 269 189 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2040 2178 2634 2040 Size: 138 456 5038 Fragments arranged by size: 5038 456 138 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5080 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4893 Fragments arranged by size: 4893 683 56 BtsI GCAGTG_nn' Cuts at: 2029 2145 3148 3942 3962 2029 Size: 116 1003 794 20 3699 Fragments arranged by size: 3699 1003 794 116 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5632 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5597 DraI TTT'AAA Cuts at: 2936 2936 Size: 5632 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4639 809 Size: 507 3323 1802 Fragments arranged by size: 3323 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2500 2623 3079 Size: 54 1425 50 275 18 670 123 456 Cuts at: 3079 3968 5597 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 670 456 275 123 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3079 1537 Size: 275 1267 4090 Fragments arranged by size: 4090 1267 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2250 2916 3562 1353 Size: 897 666 646 3423 Fragments arranged by size: 3423 897 666 646 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2179 4219 4593 4739 387 Size: 1064 138 590 2040 374 146 1280 Fragments arranged by size: 2040 1280 1064 590 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2153 2306 2411 2771 3695 4292 5079 Size: 393 521 153 105 360 924 597 787 Cuts at: 5079 5347 1239 Size: 268 1524 Fragments arranged by size: 1524 924 787 597 521 393 360 268 153 105 EcoNI CCTnn'n_nnAGG Cuts at: 2135 2135 Size: 5632 EcoO109I rG'GnC_Cy Cuts at: 2068 2811 2812 2068 Size: 743 1 4888 Fragments arranged by size: 4888 743 1 EcoRI G'AATT_C Cuts at: 2941 3319 2941 Size: 378 5254 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2292 2599 2292 Size: 307 5325 FspI TGC'GCA Cuts at: 4138 4138 Size: 5632 HaeII r_GCGC'y Cuts at: 1678 4779 1678 Size: 3101 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2292 2324 4273 4305 4347 Size: 32 135 32 720 32 1949 32 42 Cuts at: 4347 4379 1373 Size: 32 2626 Fragments arranged by size: 2626 1949 720 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2157 2976 3222 669 Size: 988 281 219 819 246 3079 Fragments arranged by size: 3079 988 819 281 246 219 HindIII A'AGCT_T Cuts at: 748 748 Size: 5632 HpaI GTT'AAC Cuts at: 3222 3222 Size: 5632 KpnI G_GTAC'C Cuts at: 2957 2957 Size: 5632 MfeI C'AATT_G Cuts at: 3231 3231 Size: 5632 MluI A'CGCG_T Cuts at: 5508 5508 Size: 5632 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4746 4930 1305 Size: 3441 184 2007 Fragments arranged by size: 3441 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3810 Fragments arranged by size: 3810 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2477 2508 3590 3949 4108 518 Size: 648 1311 31 1082 359 159 2042 Fragments arranged by size: 2042 1311 1082 648 359 159 31 MspA1I CmG'CkG Cuts at: 1606 2461 3048 3725 4873 5118 1606 Size: 855 587 677 1148 245 2120 Fragments arranged by size: 2120 1148 855 677 587 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5080 NdeI CA'TA_TG Cuts at: 387 387 Size: 5632 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5632 NotI GC'GGCC_GC Cuts at: 3079 3079 Size: 5632 NspI r_CATG'y Cuts at: 1132 1229 2990 4535 1132 Size: 97 1761 1545 2229 Fragments arranged by size: 2229 1761 1545 97 PasI CC'CwG_GG Cuts at: 1348 2071 1348 Size: 723 4909 PciI A'CATG_T Cuts at: 4531 4531 Size: 5632 PflMI CCAn_nnn'nTGG Cuts at: 1108 2731 1108 Size: 1623 4009 PfoI T'CCnGG_A Cuts at: 2283 2283 Size: 5632 PmeI GTTT'AAAC Cuts at: 2936 2936 Size: 5632 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2279 2311 3701 3733 5214 5246 2279 Size: 32 1390 32 1481 32 2665 Fragments arranged by size: 2665 1481 1390 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5632 PsiI TTA'TAA Cuts at: 3202 3202 Size: 5632 PspOMI G'GGCC_C Cuts at: 2811 2811 Size: 5632 PstI C_TGCA'G Cuts at: 830 2984 830 Size: 2154 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5632 PvuII CAG'CTG Cuts at: 2461 2461 Size: 5632 SalI G'TCGA_C Cuts at: 1936 2974 1936 Size: 1038 4594 SbfI CC_TGCA'GG Cuts at: 2984 2984 Size: 5632 ScaI AGT'ACT Cuts at: 3880 5592 3880 Size: 1712 3920 SfcI C'TryA_G Cuts at: 826 1045 2980 4115 4796 4987 826 Size: 219 1935 1135 681 191 1471 Fragments arranged by size: 1935 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5632 SmaI CCC'GGG Cuts at: 1872 2959 1872 Size: 1087 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2012 2051 2843 3736 4637 4899 Size: 197 926 69 39 792 893 901 262 Cuts at: 4899 820 Size: 1553 Fragments arranged by size: 1553 926 901 893 792 262 197 69 39 SnaBI TAC'GTA Cuts at: 493 3423 493 Size: 2930 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5632 SphI G_CATG'C Cuts at: 1132 2990 1132 Size: 1858 3774 SspI AAT'ATT Cuts at: 5 52 3556 5 Size: 47 3504 2081 Fragments arranged by size: 3504 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5632 StyI C'CwwG_G Cuts at: 513 1065 2527 2807 3071 513 Size: 552 1462 280 264 3074 Fragments arranged by size: 3074 1462 552 280 264 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2543 2738 3659 3676 3829 4014 4459 Size: 1255 963 195 921 17 153 185 445 Cuts at: 4459 325 Size: 1498 Fragments arranged by size: 1498 1255 963 921 445 195 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3878 5590 96 Size: 274 80 33 51 1443 1901 1712 138 Fragments arranged by size: 1901 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5592 5624 5592 Size: 32 5600 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5632 XbaI T'CTAG_A Cuts at: 2968 2968 Size: 5632 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5632 XmaI C'CCGG_G Cuts at: 1870 2957 1870 Size: 1087 4545 XmnI GAAnn'nnTTC Cuts at: 3761 3761 Size: 5632 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3440 276 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI