Restriction map for
pFN21AB9815
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AocI Aor51HI
ApoI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BalI BbeI
BbsI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaI BsaWI BseAI BseRI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BsrBI BsrFI BsrGI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HinfI HpaI KasI KpnI
MamI MfeI MluI MluNI MroI
MscI MseI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcyI 319 GACGCC G[GA]^CG[CT]C
AflIII 832 ACATGT A^C[GA][CT]GT
AhdI 367 GACGACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 757 GTGCAC G^TGCAC
AlwNI 436 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 673 GGGCCC GGGCC^C
ApaLI 757 GTGCAC G^TGCAC
AscI 259 GGCGCGCC GG^CGCGCC
AspEI 367 GACGACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 388 CACGTG CAC^GTG
BbvCI 69 CCTCAGC CCTCAGC(-5/-2)
BfaI 32 CTAG C^TAG
BglI 683 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 388 CACGTG [CT]AC^GT[GA]
BsaHI 319 GACGCC G[GA]^CG[CT]C
BsiEI 280 CGGCCG CG[GA][CT]^CG
BsmBI 365 GAGACG CGTCTC(1/5)
BsmFI 800 GGGAC GGGAC(10/14)
BspLU11I 832 ACATGT A^CATGT
BspMI 606 GCAGGT ACCTGC(4/8)
BsrDI 457 CATTGC GCAATG(2/0)
BstF5I 201 CATCC GGATG(2/0)
DdeI 70 CTCAG C^T[ACGT]AG
EaeI 280 CGGCCG [CT]^GGCC[GA]
EagI 280 CGGCCG C^GGCCG
Eam1105I 367 GACGACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 874 GAAGAG CTCTTC(1/4)
EclXI 280 CGGCCG C^GGCCG
Eco52I 280 CGGCCG C^GGCCG
EcoT14I 558 CCTTGG C^C[AT][AT]GG
FokI 201 CATCC GGATG(9/13)
HgaI 319 GACGC GACGC(5/10)
Hin1I 319 GACGCC G[GA]^CG[CT]C
Ksp632I 874 GAAGAG CTCTTC(1/4)
MaeI 32 CTAG C^TAG
MaeIII 544 GTCAC ^GT[ACGT]AC
MboII 874 GAAGA GAAGA(8/7)
PflMI 435 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 388 CACGTG CAC^GTG
PmlI 388 CACGTG CAC^GTG
PpuMI 437 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 72 CAGCTG CAG^CTG
ScaI 794 AGTACT AGT^ACT
SfaNI 563 GCATC GCATC(5/9)
SmaI 138 CCCGGG CCC^GGG
StyI 558 CCTTGG C^C[AT][AT]GG
Tsp45I 544 GTCAC ^GT[GC]AC
TspRI 221 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 435 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 138 CCCGGG C^CCGGG
XmaI 138 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BamHI 43 GGATCC G^GATCC
884 GGATCC
BanI 465 GGTGCC G^G[CT][GA]CC
787 GGTGCC
BcgI 406 GCAATCAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
852 GCACATGCCTCG
BsgI 385 CTGCAC GTGCAG(16/14)
850 CTGCAC
BsmAI 365 GAGAC GTCTC(1/5)
818 GAGAC
BsrI 797 ACTGG ACTGG(1/-1)
721 CCAGT
BssSI 649 CACGAG CACGAG(-5/-1)
526 CTCGTG
BstYI 43 GGATCC [GA]^GATC[CT]
884 GGATCC
DpnII 44 GATC ^GATC
885 GATC
HphI 449 TCACC GGTGA(8/7)
707 TCACC
MboI 44 GATC ^GATC
885 GATC
MflI 43 GGATCC [GA]^GATC[CT]
884 GGATCC
NdeII 44 GATC ^GATC
885 GATC
NlaIII 833 CATG CATG^
855 CATG
NspI 832 ACATGT [GA]CATG^[CT]
854 ACATGC
SacI 15 GAGCTC GAGCT^C
742 GAGCTC
Sau3AI 44 GATC ^GATC
885 GATC
TaqI 488 TCGA T^CGA
599 TCGA
TthHB8I 488 TCGA T^CGA
599 TCGA
XhoII 43 GGATCC [GA]^GATC[CT]
884 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BanII BbvI
BcnI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsoBI Bsp1286I BssHII
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DraII DsaI EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HpaII ItaI KspI
MaeII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIV PstI
RsaI SacII Sau96I ScrFI SfcI
TseI