(Circular) (Six-base) MAPSORT of: pFN21AB9815.seq Check: 8955 from: 1 to: 5602 pFN21AB9815 5602 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2602 2602 Size: 5602 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3412 278 Size: 53 83 186 936 1876 2468 Fragments arranged by size: 2468 1876 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2945 1937 Size: 1008 4594 Acc65I G'GTAC_C Cuts at: 2923 2923 Size: 5602 AclI AA'CG_TT Cuts at: 1563 3729 4102 1563 Size: 2166 373 3063 Fragments arranged by size: 3063 2166 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3665 5049 5317 1632 Size: 2033 1384 268 1917 Fragments arranged by size: 2033 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5405 AflIII A'CryG_T Cuts at: 1203 2837 4501 5478 1203 Size: 1634 1664 977 1327 Fragments arranged by size: 1664 1634 1327 977 AgeI A'CCGG_T Cuts at: 4433 4433 Size: 5602 AhdI GACnn_n'nnGTC Cuts at: 2377 4331 2377 Size: 1954 3648 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5602 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5602 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2435 2467 2435 Size: 32 5570 AlwNI CAG_nnn'CTG Cuts at: 2446 3345 4917 2446 Size: 899 1572 3131 Fragments arranged by size: 3131 1572 899 ApaI G_GGCC'C Cuts at: 2682 2682 Size: 5602 ApaLI G'TGCA_C Cuts at: 2762 3659 4815 2762 Size: 897 1156 3549 Fragments arranged by size: 3549 1156 897 ApoI r'AATT_y Cuts at: 1493 2911 3137 3289 5398 1493 Size: 1418 226 152 2109 1697 Fragments arranged by size: 2109 1697 1418 226 152 AscI GG'CGCG_CC Cuts at: 2265 2265 Size: 5602 AseI AT'TA_AT Cuts at: 160 1032 4156 160 Size: 872 3124 1606 Fragments arranged by size: 3124 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 AvaI C'yCGr_G Cuts at: 1870 1943 2017 2143 2240 2635 2690 2927 1870 Size: 73 74 126 97 395 55 237 4545 Fragments arranged by size: 4545 395 237 126 97 74 73 55 BamHI G'GATC_C Cuts at: 2048 2889 2932 3299 2048 Size: 841 43 367 4351 Fragments arranged by size: 4351 841 367 43 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2470 2792 2923 4378 618 Size: 325 217 627 683 322 131 1455 1842 Fragments arranged by size: 1842 1455 683 627 325 322 217 131 BanII G_rGCy'C Cuts at: 1391 2024 2144 2682 2751 2921 1391 Size: 633 120 538 69 170 4072 Fragments arranged by size: 4072 633 538 170 120 69 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4631 BbvCI CC'TCA_GC Cuts at: 2075 2075 Size: 5602 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2400 2434 2846 2880 3793 3827 1903 Size: 34 463 34 412 34 913 34 3678 Fragments arranged by size: 3678 913 463 412 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3494 4704 3494 Size: 1210 4392 BclI T'GATC_A Cuts at: 1548 1548 Size: 5602 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2694 4213 136 Size: 107 122 71 631 605 1022 1519 1525 Fragments arranged by size: 1525 1519 1022 631 605 122 107 71 BglII A'GATC_T Cuts at: 5597 5597 Size: 5602 BlpI GC'TnA_GC Cuts at: 3019 3019 Size: 5602 Bme1580I G_kGCm'C Cuts at: 1790 2475 2682 2699 2766 3663 4819 1790 Size: 685 207 17 67 897 1156 2573 Fragments arranged by size: 2573 1156 897 685 207 67 17 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5602 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2812 3333 4291 Size: 416 53 83 399 24 1395 521 958 Cuts at: 4291 442 Size: 1753 Fragments arranged by size: 1753 1395 958 521 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5602 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2770 2770 Size: 5602 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2468 2561 2765 4262 1239 Size: 893 336 93 204 1497 2579 Fragments arranged by size: 2579 1497 893 336 204 93 Bpu10I CC'TnA_GC Cuts at: 2075 2075 Size: 5602 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3727 4592 4890 3727 Size: 865 298 4439 Fragments arranged by size: 4439 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4265 882 Size: 617 2766 2219 Fragments arranged by size: 2766 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2395 3393 493 Size: 711 1191 998 2702 Fragments arranged by size: 2702 1191 998 711 BsaBI GATnn'nnATC Cuts at: 5596 5596 Size: 5602 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2325 3409 3791 4426 Size: 53 83 186 936 792 1084 382 635 Cuts at: 4426 275 Size: 1451 Fragments arranged by size: 1451 1084 936 792 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4035 4433 4707 4854 5265 4035 Size: 398 274 147 411 4372 Fragments arranged by size: 4372 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5377 Fragments arranged by size: 5377 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5602 BseYI C'CCAG_C Cuts at: 2231 4805 5301 2231 Size: 2574 496 2532 Fragments arranged by size: 2574 2532 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2375 2840 1163 Size: 573 639 465 3925 Fragments arranged by size: 3925 639 573 465 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2288 3052 3813 4841 1540 Size: 275 186 287 764 761 1028 2301 Fragments arranged by size: 2301 1028 764 761 287 275 186 BsiHKAI G_wGCw'C Cuts at: 2024 2751 2766 3663 3748 4819 2024 Size: 727 15 897 85 1071 2807 Fragments arranged by size: 2807 1071 897 727 85 15 BsmI GAATG_Cn' Cuts at: 3113 3206 3113 Size: 93 5509 BsmBI CGTCTCn'nnnn_ Cuts at: 2364 2364 Size: 5602 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2144 2475 2682 2699 2751 2766 Size: 399 234 120 331 207 17 52 15 Cuts at: 2766 2921 3663 3748 4819 1391 Size: 155 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 331 234 207 155 120 85 52 17 15 BspEI T'CCGG_A Cuts at: 5265 5265 Size: 5602 BspHI T'CATG_A Cuts at: 3491 5221 3491 Size: 1730 3872 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2602 2957 844 Size: 1758 355 3489 Fragments arranged by size: 3489 1758 355 BsrBI CCG'CTC Cuts at: 1394 1823 3489 1394 Size: 429 1666 3507 Fragments arranged by size: 3507 1666 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2461 4097 4271 65 Size: 1183 1213 1636 174 1396 Fragments arranged by size: 1636 1396 1213 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4246 4433 1700 Size: 110 2436 187 2869 Fragments arranged by size: 2869 2436 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5602 BssHII G'CGCG_C Cuts at: 1925 2151 2219 2265 1925 Size: 226 68 46 5262 Fragments arranged by size: 5262 226 68 46 BssSI C'ACGA_G Cuts at: 2531 2654 3662 4674 2531 Size: 123 1008 1012 3459 Fragments arranged by size: 3459 1012 1008 123 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5602 BstBI TT'CG_AA Cuts at: 4406 4406 Size: 5602 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5602 BstYI r'GATC_y Cuts at: 1972 2048 2889 2932 3299 3684 3701 5153 5239 Size: 76 841 43 367 385 17 1452 86 Cuts at: 5239 5251 5597 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 841 385 367 346 86 76 43 17 12 BtgI C'CryG_G Cuts at: 513 1065 2025 2117 2259 513 Size: 552 960 92 142 3856 Fragments arranged by size: 3856 960 552 142 92 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4863 Fragments arranged by size: 4863 683 56 BtsI GCAGTG_nn' Cuts at: 2225 3118 3912 3932 2225 Size: 893 794 20 3895 Fragments arranged by size: 3895 893 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5602 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5567 DraI TTT'AAA Cuts at: 2906 2906 Size: 5602 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4609 809 Size: 507 3293 1802 Fragments arranged by size: 3293 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2285 3049 3938 Size: 54 1425 50 275 18 455 764 889 Cuts at: 3938 5567 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 764 455 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2285 3049 1537 Size: 275 473 764 4090 Fragments arranged by size: 4090 764 473 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2874 3532 1353 Size: 1521 658 3423 Fragments arranged by size: 3423 1521 658 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4189 4563 4709 387 Size: 1064 138 2600 374 146 1280 Fragments arranged by size: 2600 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2022 2749 2022 Size: 727 4875 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 2468 2561 2765 3665 4262 5049 Size: 393 500 336 93 204 900 597 787 Cuts at: 5049 5317 1239 Size: 268 1524 Fragments arranged by size: 1524 900 787 597 500 393 336 268 204 93 EcoO109I rG'GnC_Cy Cuts at: 2130 2443 2687 2130 Size: 313 244 5045 Fragments arranged by size: 5045 313 244 EcoRI G'AATT_C Cuts at: 2911 3289 2911 Size: 378 5224 FspI TGC'GCA Cuts at: 4108 4108 Size: 5602 HaeII r_GCGC'y Cuts at: 1678 2043 2174 2248 2277 2518 4749 1678 Size: 365 131 74 29 241 2231 2531 Fragments arranged by size: 2531 2231 365 241 131 74 29 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2363 2395 4243 4275 4317 Size: 32 135 32 791 32 1848 32 42 Cuts at: 4317 4349 1373 Size: 32 2626 Fragments arranged by size: 2626 1848 791 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2946 3192 669 Size: 988 281 1008 246 3079 Fragments arranged by size: 3079 1008 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5602 HpaI GTT'AAC Cuts at: 3192 3192 Size: 5602 KpnI G_GTAC'C Cuts at: 2927 2927 Size: 5602 MfeI C'AATT_G Cuts at: 3201 3201 Size: 5602 MluI A'CGCG_T Cuts at: 5478 5478 Size: 5602 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2067 4716 4900 1305 Size: 762 2649 184 2007 Fragments arranged by size: 2649 2007 762 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3780 Fragments arranged by size: 3780 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3560 3919 4078 518 Size: 648 2394 359 159 2042 Fragments arranged by size: 2394 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2027 2079 2119 2261 2389 2524 2574 3018 Size: 421 52 40 142 128 135 50 444 Cuts at: 3018 3695 4843 5088 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 444 421 245 142 135 128 52 50 40 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5050 NdeI CA'TA_TG Cuts at: 387 387 Size: 5602 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5602 NotI GC'GGCC_GC Cuts at: 3049 3049 Size: 5602 NspI r_CATG'y Cuts at: 1132 1229 2841 2863 2960 4505 1132 Size: 97 1612 22 97 1545 2229 Fragments arranged by size: 2229 1612 1545 97 97 22 PasI CC'CwG_GG Cuts at: 1348 2044 2135 2329 1348 Size: 696 91 194 4621 Fragments arranged by size: 4621 696 194 91 PciI A'CATG_T Cuts at: 2837 4501 2837 Size: 1664 3938 PflMI CCAn_nnn'nTGG Cuts at: 1108 2446 1108 Size: 1338 4264 PfoI T'CCnGG_A Cuts at: 2051 2051 Size: 5602 PmeI GTTT'AAAC Cuts at: 2906 2906 Size: 5602 PmlI CAC'GTG Cuts at: 2395 2395 Size: 5602 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3671 3703 5184 5216 3671 Size: 32 1481 32 4057 Fragments arranged by size: 4057 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2443 2443 Size: 5602 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5602 PsiI TTA'TAA Cuts at: 3172 3172 Size: 5602 PspOMI G'GGCC_C Cuts at: 2678 2678 Size: 5602 PstI C_TGCA'G Cuts at: 830 2297 2435 2523 2954 830 Size: 1467 138 88 431 3478 Fragments arranged by size: 3478 1467 431 138 88 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5602 PvuII CAG'CTG Cuts at: 2079 2079 Size: 5602 SacI G_AGCT'C Cuts at: 2024 2751 2024 Size: 727 4875 SacII CC_GC'GG Cuts at: 2028 2120 2262 2028 Size: 92 142 5368 Fragments arranged by size: 5368 142 92 SalI G'TCGA_C Cuts at: 1936 2944 1936 Size: 1008 4594 SbfI CC_TGCA'GG Cuts at: 2954 2954 Size: 5602 ScaI AGT'ACT Cuts at: 2801 3850 5562 2801 Size: 1049 1712 2841 Fragments arranged by size: 2841 1712 1049 SfcI C'TryA_G Cuts at: 826 1045 2125 2293 2431 2519 2827 2950 4085 Size: 219 1080 168 138 88 308 123 1135 Cuts at: 4085 4766 4957 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1080 681 308 219 191 168 138 123 88 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5602 SmaI CCC'GGG Cuts at: 1872 2145 2929 1872 Size: 273 784 4545 Fragments arranged by size: 4545 784 273 SmlI C'TyrA_G Cuts at: 820 1017 1943 3706 4607 4869 820 Size: 197 926 1763 901 262 1553 Fragments arranged by size: 1763 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3393 493 Size: 2900 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5602 SphI G_CATG'C Cuts at: 1132 2960 1132 Size: 1828 3774 SrfI GCCC'GGGC Cuts at: 2145 2145 Size: 5602 SspI AAT'ATT Cuts at: 5 52 3526 5 Size: 47 3474 2081 Fragments arranged by size: 3474 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5602 StyI C'CwwG_G Cuts at: 513 1065 2563 3041 513 Size: 552 1498 478 3074 Fragments arranged by size: 3074 1498 552 478 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3629 3646 3799 3984 4429 325 Size: 1255 2049 17 153 185 445 1498 Fragments arranged by size: 2049 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2799 3848 5560 Size: 274 80 33 51 1443 822 1049 1712 Cuts at: 5560 96 Size: 138 Fragments arranged by size: 1712 1443 1049 822 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5562 5594 5562 Size: 32 5570 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5602 XbaI T'CTAG_A Cuts at: 2938 2938 Size: 5602 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5602 XmaI C'CCGG_G Cuts at: 1870 2143 2927 1870 Size: 273 784 4545 Fragments arranged by size: 4545 784 273 XmnI GAAnn'nnTTC Cuts at: 3731 3731 Size: 5602 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3410 276 Size: 53 83 186 936 1876 2468 Fragments arranged by size: 2468 1876 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SwaI XcmI