(Circular) (Six-base) MAPSORT of: pFN21AB9814.seq Check: 494 from: 1 to: 5587 pFN21AB9814 5587 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3397 278 Size: 53 83 186 936 1861 2468 Fragments arranged by size: 2468 1861 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2930 1937 Size: 993 4594 Acc65I G'GTAC_C Cuts at: 2908 2908 Size: 5587 AclI AA'CG_TT Cuts at: 1563 3714 4087 1563 Size: 2151 373 3063 Fragments arranged by size: 3063 2151 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2421 3650 5034 5302 1632 Size: 789 1229 1384 268 1917 Fragments arranged by size: 1917 1384 1229 789 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5390 AflIII A'CryG_T Cuts at: 1203 4486 5463 1203 Size: 3283 977 1327 Fragments arranged by size: 3283 1327 977 AgeI A'CCGG_T Cuts at: 4418 4418 Size: 5587 AhdI GACnn_n'nnGTC Cuts at: 2414 4316 2414 Size: 1902 3685 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5587 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5587 AlwNI CAG_nnn'CTG Cuts at: 3330 4902 3330 Size: 1572 4015 ApaI G_GGCC'C Cuts at: 2519 2519 Size: 5587 ApaLI G'TGCA_C Cuts at: 3644 4800 3644 Size: 1156 4431 ApoI r'AATT_y Cuts at: 1493 2116 2896 3122 3274 5383 1493 Size: 623 780 226 152 2109 1697 Fragments arranged by size: 2109 1697 780 623 226 152 AseI AT'TA_AT Cuts at: 160 1032 4141 160 Size: 872 3109 1606 Fragments arranged by size: 3109 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5587 AvaI C'yCGr_G Cuts at: 1870 1943 2185 2872 2912 1870 Size: 73 242 687 40 4545 Fragments arranged by size: 4545 687 242 73 40 BamHI G'GATC_C Cuts at: 2917 3284 2917 Size: 367 5220 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2375 2908 4363 618 Size: 325 217 627 588 533 1455 1842 Fragments arranged by size: 1842 1455 627 588 533 325 217 BanII G_rGCy'C Cuts at: 1391 2238 2434 2497 2519 2700 2799 2906 1391 Size: 847 196 63 22 181 99 107 4072 Fragments arranged by size: 4072 847 196 181 107 99 63 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4616 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3778 3812 1903 Size: 34 1841 34 3678 Fragments arranged by size: 3678 1841 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3479 4689 3479 Size: 1210 4377 BclI T'GATC_A Cuts at: 1548 1548 Size: 5587 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2046 2588 4198 Size: 107 122 71 631 605 374 542 1610 Cuts at: 4198 136 Size: 1525 Fragments arranged by size: 1610 1525 631 605 542 374 122 107 71 BglII A'GATC_T Cuts at: 2535 5582 2535 Size: 3047 2540 BlpI GC'TnA_GC Cuts at: 3004 3004 Size: 5587 Bme1580I G_kGCm'C Cuts at: 1790 2143 2519 3648 4804 1790 Size: 353 376 1129 1156 2573 Fragments arranged by size: 2573 1156 1129 376 353 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5587 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2140 2639 2833 Size: 416 53 83 399 24 723 499 194 Cuts at: 2833 3318 4276 442 Size: 485 958 1753 Fragments arranged by size: 1753 958 723 499 485 416 399 194 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5587 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2342 2349 2585 4247 1239 Size: 1103 7 236 1662 2579 Fragments arranged by size: 2579 1662 1103 236 7 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2261 2289 3712 4577 4875 2261 Size: 28 1423 865 298 2973 Fragments arranged by size: 2973 1423 865 298 28 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2377 2762 2862 4250 882 Size: 617 878 385 100 1388 2219 Fragments arranged by size: 2219 1388 878 617 385 100 BsaAI yAC'GTr Cuts at: 493 1204 3378 493 Size: 711 2174 2702 Fragments arranged by size: 2702 2174 711 BsaBI GATnn'nnATC Cuts at: 5581 5581 Size: 5587 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3394 3776 4411 275 Size: 53 83 186 936 1861 382 635 1451 Fragments arranged by size: 1861 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4020 4418 4692 4839 5250 4020 Size: 398 274 147 411 4357 Fragments arranged by size: 4357 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5362 Fragments arranged by size: 5362 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2098 2246 2870 1186 Size: 912 148 624 3903 Fragments arranged by size: 3903 912 624 148 BseYI C'CCAG_C Cuts at: 2647 4790 5286 2647 Size: 2143 496 2948 Fragments arranged by size: 2948 2143 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5014 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3037 3798 4826 1540 Size: 275 186 1036 761 1028 2301 Fragments arranged by size: 2301 1036 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2497 2600 3648 3733 4804 2497 Size: 103 1048 85 1071 3280 Fragments arranged by size: 3280 1071 1048 103 85 BsmI GAATG_Cn' Cuts at: 3098 3191 3098 Size: 93 5494 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2143 2238 2434 2497 2519 2600 2700 Size: 399 353 95 196 63 22 81 100 Cuts at: 2700 2799 2906 3648 3733 4804 1391 Size: 99 107 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 353 196 107 100 99 95 85 81 63 22 BspEI T'CCGG_A Cuts at: 5250 5250 Size: 5587 BspHI T'CATG_A Cuts at: 3476 5206 3476 Size: 1730 3857 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2942 844 Size: 2098 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3474 1394 Size: 429 1651 3507 Fragments arranged by size: 3507 1651 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4082 4256 65 Size: 1183 2834 174 1396 Fragments arranged by size: 2834 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4231 4418 1700 Size: 110 2421 187 2869 Fragments arranged by size: 2869 2421 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5587 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5587 BssSI C'ACGA_G Cuts at: 3647 4659 3647 Size: 1012 4575 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5587 BstBI TT'CG_AA Cuts at: 4391 4391 Size: 5587 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5587 BstXI CCAn_nnnn'nTGG Cuts at: 2098 2098 Size: 5587 BstYI r'GATC_y Cuts at: 1972 2535 2917 3284 3669 3686 5138 5224 5236 Size: 563 382 367 385 17 1452 86 12 Cuts at: 5236 5582 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 563 385 382 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2151 513 Size: 552 1086 3949 Fragments arranged by size: 3949 1086 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4848 Fragments arranged by size: 4848 683 56 BtsI GCAGTG_nn' Cuts at: 2606 3103 3897 3917 2606 Size: 497 794 20 4276 Fragments arranged by size: 4276 794 497 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5587 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5552 DraI TTT'AAA Cuts at: 2891 2891 Size: 5587 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4594 809 Size: 507 3278 1802 Fragments arranged by size: 3278 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2814 3034 3923 Size: 54 1425 50 275 18 984 220 889 Cuts at: 3923 5552 8 Size: 1629 43 Fragments arranged by size: 1629 1425 984 889 275 220 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3034 1537 Size: 275 1222 4090 Fragments arranged by size: 4090 1222 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2517 3517 1353 Size: 1164 1000 3423 Fragments arranged by size: 3423 1164 1000 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4174 4548 4694 387 Size: 1064 138 2585 374 146 1280 Fragments arranged by size: 2585 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2495 2495 Size: 5587 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2342 2349 2421 2585 3650 4247 5034 Size: 393 710 7 72 164 1065 597 787 Cuts at: 5034 5302 1239 Size: 268 1524 Fragments arranged by size: 1524 1065 787 710 597 393 268 164 72 7 EcoNI CCTnn'n_nnAGG Cuts at: 2424 2424 Size: 5587 EcoO109I rG'GnC_Cy Cuts at: 2515 2515 Size: 5587 EcoRI G'AATT_C Cuts at: 2116 2896 3274 2116 Size: 780 378 4429 Fragments arranged by size: 4429 780 378 FspI TGC'GCA Cuts at: 4093 4093 Size: 5587 HaeII r_GCGC'y Cuts at: 1678 4734 1678 Size: 3056 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2093 2125 2400 2432 2542 Size: 32 135 32 521 32 275 32 110 Cuts at: 2542 2574 4228 4260 4302 4334 1373 Size: 32 1654 32 42 32 2626 Fragments arranged by size: 2626 1654 521 275 135 110 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2931 3177 669 Size: 988 281 993 246 3079 Fragments arranged by size: 3079 993 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5587 HpaI GTT'AAC Cuts at: 3177 3177 Size: 5587 KpnI G_GTAC'C Cuts at: 2912 2912 Size: 5587 MfeI C'AATT_G Cuts at: 3186 3186 Size: 5587 MluI A'CGCG_T Cuts at: 5463 5463 Size: 5587 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2820 4701 4885 1305 Size: 1515 1881 184 2007 Fragments arranged by size: 2007 1881 1515 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3765 Fragments arranged by size: 3765 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3545 3904 4063 518 Size: 648 2379 359 159 2042 Fragments arranged by size: 2379 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2647 3003 3680 4828 5073 1606 Size: 1041 356 677 1148 245 2120 Fragments arranged by size: 2120 1148 1041 677 356 245 NcoI C'CATG_G Cuts at: 513 1065 2151 513 Size: 552 1086 3949 Fragments arranged by size: 3949 1086 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5587 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5587 NotI GC'GGCC_GC Cuts at: 3034 3034 Size: 5587 NspI r_CATG'y Cuts at: 1132 1229 2945 4490 1132 Size: 97 1716 1545 2229 Fragments arranged by size: 2229 1716 1545 97 PasI CC'CwG_GG Cuts at: 1348 2420 1348 Size: 1072 4515 PciI A'CATG_T Cuts at: 4486 4486 Size: 5587 PflMI CCAn_nnn'nTGG Cuts at: 1108 2823 1108 Size: 1715 3872 PfoI T'CCnGG_A Cuts at: 2790 2790 Size: 5587 PmeI GTTT'AAAC Cuts at: 2891 2891 Size: 5587 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3656 3688 5169 5201 3656 Size: 32 1481 32 4042 Fragments arranged by size: 4042 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5587 PsiI TTA'TAA Cuts at: 3157 3157 Size: 5587 PspOMI G'GGCC_C Cuts at: 2515 2515 Size: 5587 PstI C_TGCA'G Cuts at: 830 2535 2766 2939 830 Size: 1705 231 173 3478 Fragments arranged by size: 3478 1705 231 173 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5587 SacI G_AGCT'C Cuts at: 2497 2497 Size: 5587 SalI G'TCGA_C Cuts at: 1936 2929 1936 Size: 993 4594 SbfI CC_TGCA'GG Cuts at: 2766 2939 2766 Size: 173 5414 ScaI AGT'ACT Cuts at: 3835 5547 3835 Size: 1712 3875 SexAI A'CCwGG_T Cuts at: 2446 2446 Size: 5587 SfcI C'TryA_G Cuts at: 826 1045 2531 2762 2935 4070 4751 4942 826 Size: 219 1486 231 173 1135 681 191 1471 Fragments arranged by size: 1486 1471 1135 681 231 219 191 173 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5587 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5587 SmaI CCC'GGG Cuts at: 1872 2874 2914 1872 Size: 1002 40 4545 Fragments arranged by size: 4545 1002 40 SmlI C'TyrA_G Cuts at: 820 1017 1943 2276 2304 3691 4592 4854 820 Size: 197 926 333 28 1387 901 262 1553 Fragments arranged by size: 1553 1387 926 901 333 262 197 28 SnaBI TAC'GTA Cuts at: 493 3378 493 Size: 2885 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5587 SphI G_CATG'C Cuts at: 1132 2945 1132 Size: 1813 3774 SspI AAT'ATT Cuts at: 5 52 3511 5 Size: 47 3459 2081 Fragments arranged by size: 3459 2081 47 StuI AGG'CCT Cuts at: 1517 2068 2768 1517 Size: 551 700 4336 Fragments arranged by size: 4336 700 551 StyI C'CwwG_G Cuts at: 513 1065 2042 2151 2619 2769 3026 513 Size: 552 977 109 468 150 257 3074 Fragments arranged by size: 3074 977 552 468 257 150 109 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3614 3631 3784 3969 4414 325 Size: 1255 2034 17 153 185 445 1498 Fragments arranged by size: 2034 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3833 5545 96 Size: 274 80 33 51 1443 1856 1712 138 Fragments arranged by size: 1856 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5547 5579 5547 Size: 32 5555 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5587 XbaI T'CTAG_A Cuts at: 2923 2923 Size: 5587 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2158 2158 Size: 5587 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5587 XmaI C'CCGG_G Cuts at: 1870 2872 2912 1870 Size: 1002 40 4545 Fragments arranged by size: 4545 1002 40 XmnI GAAnn'nnTTC Cuts at: 2269 3716 2269 Size: 1447 4140 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3395 276 Size: 53 83 186 936 1861 2468 Fragments arranged by size: 2468 1861 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI