(Circular) (Six-base) MAPSORT of: pFN21AB9812.seq Check: 9104 from: 1 to: 5476 pFN21AB9812 5476 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3286 278 Size: 53 83 186 936 1750 2468 Fragments arranged by size: 2468 1750 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2819 1937 Size: 882 4594 Acc65I G'GTAC_C Cuts at: 2797 2797 Size: 5476 AclI AA'CG_TT Cuts at: 1563 3603 3976 1563 Size: 2040 373 3063 Fragments arranged by size: 3063 2040 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2673 3539 4923 5191 1632 Size: 1041 866 1384 268 1917 Fragments arranged by size: 1917 1384 1041 866 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5279 AflIII A'CryG_T Cuts at: 1203 2733 4375 5352 1203 Size: 1530 1642 977 1327 Fragments arranged by size: 1642 1530 1327 977 AgeI A'CCGG_T Cuts at: 4307 4307 Size: 5476 AhdI GACnn_n'nnGTC Cuts at: 2519 4205 2519 Size: 1686 3790 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5476 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5476 AlwNI CAG_nnn'CTG Cuts at: 3219 4791 3219 Size: 1572 3904 ApaI G_GGCC'C Cuts at: 2235 2235 Size: 5476 ApaLI G'TGCA_C Cuts at: 3533 4689 3533 Size: 1156 4320 ApoI r'AATT_y Cuts at: 1493 2785 3011 3163 5272 1493 Size: 1292 226 152 2109 1697 Fragments arranged by size: 2109 1697 1292 226 152 AseI AT'TA_AT Cuts at: 160 1032 4030 160 Size: 872 2998 1606 Fragments arranged by size: 2998 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5476 AvaI C'yCGr_G Cuts at: 1870 1943 2211 2801 1870 Size: 73 268 590 4545 Fragments arranged by size: 4545 590 268 73 BamHI G'GATC_C Cuts at: 2806 3173 2806 Size: 367 5109 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2797 4252 618 Size: 325 217 627 1010 1455 1842 Fragments arranged by size: 1842 1455 1010 627 325 217 BanII G_rGCy'C Cuts at: 1391 2235 2346 2795 1391 Size: 844 111 449 4072 Fragments arranged by size: 4072 844 449 111 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2094 928 Size: 971 195 4310 Fragments arranged by size: 4310 971 195 BbvCI CC'TCA_GC Cuts at: 2202 2202 Size: 5476 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3667 3701 1903 Size: 34 1730 34 3678 Fragments arranged by size: 3678 1730 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2715 3368 4578 2715 Size: 653 1210 3613 Fragments arranged by size: 3613 1210 653 BclI T'GATC_A Cuts at: 1548 1548 Size: 5476 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4087 136 Size: 107 122 71 631 605 2415 1525 Fragments arranged by size: 2415 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5471 5471 Size: 5476 BlpI GC'TnA_GC Cuts at: 2321 2893 2321 Size: 572 4904 Bme1580I G_kGCm'C Cuts at: 1790 2235 3537 4693 1790 Size: 445 1302 1156 2573 Fragments arranged by size: 2573 1302 1156 445 BmgBI CAC'GTC Cuts at: 1318 2736 1318 Size: 1418 4058 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3207 4165 442 Size: 416 53 83 399 24 1790 958 1753 Fragments arranged by size: 1790 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5476 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4136 1239 Size: 2897 2579 Bpu10I CC'TnA_GC Cuts at: 2202 2202 Size: 5476 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3601 4466 4764 3601 Size: 865 298 4313 Fragments arranged by size: 4313 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4139 882 Size: 617 2640 2219 Fragments arranged by size: 2640 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2374 3267 493 Size: 711 1170 893 2702 Fragments arranged by size: 2702 1170 893 711 BsaBI GATnn'nnATC Cuts at: 5470 5470 Size: 5476 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3283 3665 4300 275 Size: 53 83 186 936 1750 382 635 1451 Fragments arranged by size: 1750 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3909 4307 4581 4728 5139 3909 Size: 398 274 147 411 4246 Fragments arranged by size: 4246 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2132 2162 1178 Size: 30 165 30 729 30 4492 Fragments arranged by size: 4492 729 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5476 BseYI C'CCAG_C Cuts at: 2530 4679 5175 2530 Size: 2149 496 2831 Fragments arranged by size: 2831 2149 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4903 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2926 3687 4715 1540 Size: 275 186 925 761 1028 2301 Fragments arranged by size: 2301 1028 925 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2189 2683 3537 3622 4693 2189 Size: 494 854 85 1071 2972 Fragments arranged by size: 2972 1071 854 494 85 BsmI GAATG_Cn' Cuts at: 2246 2987 3080 2246 Size: 741 93 4642 Fragments arranged by size: 4642 741 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2287 2287 Size: 5476 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2189 2235 2346 2683 2795 3537 3622 Size: 399 399 46 111 337 112 742 85 Cuts at: 3622 4693 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 399 337 112 111 85 46 BspEI T'CCGG_A Cuts at: 5139 5139 Size: 5476 BspHI T'CATG_A Cuts at: 3365 5095 3365 Size: 1730 3746 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2831 844 Size: 1987 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2224 3363 1394 Size: 429 401 1139 3507 Fragments arranged by size: 3507 1139 429 401 BsrDI GCAATG_nn' Cuts at: 65 1248 2271 3971 4145 65 Size: 1183 1023 1700 174 1396 Fragments arranged by size: 1700 1396 1183 1023 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2602 4120 4307 1700 Size: 110 792 1518 187 2869 Fragments arranged by size: 2869 1518 792 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5476 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5476 BssSI C'ACGA_G Cuts at: 2705 3536 4548 2705 Size: 831 1012 3633 Fragments arranged by size: 3633 1012 831 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5476 BstBI TT'CG_AA Cuts at: 4280 4280 Size: 5476 BstEII G'GTnAC_C Cuts at: 1184 2479 1184 Size: 1295 4181 BstXI CCAn_nnnn'nTGG Cuts at: 2158 2158 Size: 5476 BstYI r'GATC_y Cuts at: 1972 2806 3173 3558 3575 5027 5113 5125 5471 Size: 834 367 385 17 1452 86 12 346 Cuts at: 5471 1972 Size: 1977 Fragments arranged by size: 1977 1452 834 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2044 2309 2385 513 Size: 552 979 265 76 3604 Fragments arranged by size: 3604 979 552 265 76 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2599 487 Size: 683 56 1373 3364 Fragments arranged by size: 3364 1373 683 56 BtsI GCAGTG_nn' Cuts at: 2992 3786 3806 2992 Size: 794 20 4662 Fragments arranged by size: 4662 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5476 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5441 DraI TTT'AAA Cuts at: 2780 2780 Size: 5476 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2488 4483 809 Size: 507 1172 1995 1802 Fragments arranged by size: 1995 1802 1172 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2496 2923 3812 Size: 54 1425 50 275 18 666 427 889 Cuts at: 3812 5441 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 666 427 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2923 1537 Size: 275 1111 4090 Fragments arranged by size: 4090 1111 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3406 1353 Size: 2053 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2425 4063 4437 4583 387 Size: 1064 138 836 1638 374 146 1280 Fragments arranged by size: 1638 1280 1064 836 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2673 3539 4136 4923 5191 1239 Size: 393 1041 866 597 787 268 1524 Fragments arranged by size: 1524 1041 866 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2231 2314 2231 Size: 83 5393 EcoRI G'AATT_C Cuts at: 2785 3163 2785 Size: 378 5098 FspI TGC'GCA Cuts at: 3982 3982 Size: 5476 HaeII r_GCGC'y Cuts at: 1678 4623 1678 Size: 2945 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2477 2505 2509 2537 4117 Size: 32 135 32 905 28 4 28 1580 Cuts at: 4117 4149 4191 4223 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1580 905 135 42 32 32 32 32 28 28 4 HincII GTy'rAC Cuts at: 669 1657 1938 2820 3066 669 Size: 988 281 882 246 3079 Fragments arranged by size: 3079 988 882 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5476 HpaI GTT'AAC Cuts at: 3066 3066 Size: 5476 KpnI G_GTAC'C Cuts at: 2801 2801 Size: 5476 MfeI C'AATT_G Cuts at: 3075 3075 Size: 5476 MluI A'CGCG_T Cuts at: 5352 5352 Size: 5476 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4590 4774 1305 Size: 3285 184 2007 Fragments arranged by size: 3285 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3654 Fragments arranged by size: 3654 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3434 3793 3952 518 Size: 648 2268 359 159 2042 Fragments arranged by size: 2268 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2311 2892 3569 4717 4962 1606 Size: 705 581 677 1148 245 2120 Fragments arranged by size: 2120 1148 705 677 581 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4924 NdeI CA'TA_TG Cuts at: 387 387 Size: 5476 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5476 NotI GC'GGCC_GC Cuts at: 2923 2923 Size: 5476 NspI r_CATG'y Cuts at: 1132 1229 2834 4379 1132 Size: 97 1605 1545 2229 Fragments arranged by size: 2229 1605 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5476 PciI A'CATG_T Cuts at: 4375 4375 Size: 5476 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5476 PmeI GTTT'AAAC Cuts at: 2780 2780 Size: 5476 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3545 3577 5058 5090 3545 Size: 32 1481 32 3931 Fragments arranged by size: 3931 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2314 2314 Size: 5476 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5476 PsiI TTA'TAA Cuts at: 3046 3046 Size: 5476 PspOMI G'GGCC_C Cuts at: 2231 2231 Size: 5476 PstI C_TGCA'G Cuts at: 830 2828 830 Size: 1998 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5476 SacII CC_GC'GG Cuts at: 2312 2312 Size: 5476 SalI G'TCGA_C Cuts at: 1936 2818 1936 Size: 882 4594 SbfI CC_TGCA'GG Cuts at: 2828 2828 Size: 5476 ScaI AGT'ACT Cuts at: 2450 3724 5436 2450 Size: 1274 1712 2490 Fragments arranged by size: 2490 1712 1274 SexAI A'CCwGG_T Cuts at: 2517 2517 Size: 5476 SfcI C'TryA_G Cuts at: 826 1045 2824 3959 4640 4831 826 Size: 219 1779 1135 681 191 1471 Fragments arranged by size: 1779 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5476 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5476 SmaI CCC'GGG Cuts at: 1872 2803 1872 Size: 931 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3580 4481 4743 820 Size: 197 926 1637 901 262 1553 Fragments arranged by size: 1637 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3267 493 Size: 2774 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5476 SphI G_CATG'C Cuts at: 1132 2834 1132 Size: 1702 3774 SspI AAT'ATT Cuts at: 5 52 2570 3400 5 Size: 47 2518 830 2081 Fragments arranged by size: 2518 2081 830 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5476 StyI C'CwwG_G Cuts at: 513 1065 2053 2337 2915 513 Size: 552 988 284 578 3074 Fragments arranged by size: 3074 988 578 552 284 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2467 2498 3503 3520 3673 3858 4303 Size: 1255 887 31 1005 17 153 185 445 Cuts at: 4303 325 Size: 1498 Fragments arranged by size: 1498 1255 1005 887 445 185 153 31 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2448 3722 5434 Size: 274 80 33 51 1443 471 1274 1712 Cuts at: 5434 96 Size: 138 Fragments arranged by size: 1712 1443 1274 471 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2298 2330 5436 5468 2298 Size: 32 3106 32 2306 Fragments arranged by size: 3106 2306 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5476 XbaI T'CTAG_A Cuts at: 2812 2812 Size: 5476 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2158 2158 Size: 5476 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5476 XmaI C'CCGG_G Cuts at: 1870 2801 1870 Size: 931 4545 XmnI GAAnn'nnTTC Cuts at: 3605 3605 Size: 5476 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3284 276 Size: 53 83 186 936 1750 2468 Fragments arranged by size: 2468 1750 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI