(Circular) (Six-base) MAPSORT of: pFN21AB9809.seq Check: 6659 from: 1 to: 5428 pFN21AB9809 5428 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2415 2415 Size: 5428 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3238 278 Size: 53 83 186 936 1702 2468 Fragments arranged by size: 2468 1702 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2771 1937 Size: 834 4594 Acc65I G'GTAC_C Cuts at: 2749 2749 Size: 5428 AclI AA'CG_TT Cuts at: 1563 3555 3928 1563 Size: 1992 373 3063 Fragments arranged by size: 3063 1992 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2223 2629 3491 4875 5143 1632 Size: 591 406 862 1384 268 1917 Fragments arranged by size: 1917 1384 862 591 406 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5231 AflIII A'CryG_T Cuts at: 1203 4327 5304 1203 Size: 3124 977 1327 Fragments arranged by size: 3124 1327 977 AgeI A'CCGG_T Cuts at: 4259 4259 Size: 5428 AhdI GACnn_n'nnGTC Cuts at: 4157 4157 Size: 5428 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5428 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2672 1184 Size: 1488 3940 AlwNI CAG_nnn'CTG Cuts at: 3171 4743 3171 Size: 1572 3856 ApaI G_GGCC'C Cuts at: 2448 2448 Size: 5428 ApaLI G'TGCA_C Cuts at: 3485 4641 3485 Size: 1156 4272 ApoI r'AATT_y Cuts at: 1493 2737 2963 3115 5224 1493 Size: 1244 226 152 2109 1697 Fragments arranged by size: 2109 1697 1244 226 152 AseI AT'TA_AT Cuts at: 160 1032 3982 160 Size: 872 2950 1606 Fragments arranged by size: 2950 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5428 AvaI C'yCGr_G Cuts at: 1870 1943 2753 1870 Size: 73 810 4545 Fragments arranged by size: 4545 810 73 BamHI G'GATC_C Cuts at: 2758 3125 2758 Size: 367 5061 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2749 4204 618 Size: 325 217 627 962 1455 1842 Fragments arranged by size: 1842 1455 962 627 325 217 BanII G_rGCy'C Cuts at: 1391 2448 2747 1391 Size: 1057 299 4072 Fragments arranged by size: 4072 1057 299 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2075 2276 2598 928 Size: 971 176 201 322 3758 Fragments arranged by size: 3758 971 322 201 176 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3619 3653 1903 Size: 34 1682 34 3678 Fragments arranged by size: 3678 1682 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3320 4530 3320 Size: 1210 4218 BclI T'GATC_A Cuts at: 1548 1548 Size: 5428 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2482 4039 Size: 107 122 71 631 605 337 473 1557 Cuts at: 4039 136 Size: 1525 Fragments arranged by size: 1557 1525 631 605 473 337 122 107 71 BglII A'GATC_T Cuts at: 5423 5423 Size: 5428 BlpI GC'TnA_GC Cuts at: 2845 2845 Size: 5428 Bme1580I G_kGCm'C Cuts at: 1790 2448 3489 4645 1790 Size: 658 1041 1156 2573 Fragments arranged by size: 2573 1156 1041 658 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5428 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2442 3159 4117 Size: 416 53 83 399 24 1025 717 958 Cuts at: 4117 442 Size: 1753 Fragments arranged by size: 1753 1025 958 717 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5428 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2595 2610 2717 4088 1239 Size: 1356 15 107 1371 2579 Fragments arranged by size: 2579 1371 1356 107 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3553 4418 4716 3553 Size: 865 298 4265 Fragments arranged by size: 4265 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2027 4091 882 Size: 617 528 2064 2219 Fragments arranged by size: 2219 2064 617 528 BsaAI yAC'GTr Cuts at: 493 1204 3219 493 Size: 711 2015 2702 Fragments arranged by size: 2702 2015 711 BsaBI GATnn'nnATC Cuts at: 5422 5422 Size: 5428 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2423 3235 3617 4252 Size: 53 83 186 936 890 812 382 635 Cuts at: 4252 275 Size: 1451 Fragments arranged by size: 1451 936 890 812 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2527 2555 3861 4259 4533 4680 5091 2527 Size: 28 1306 398 274 147 411 2864 Fragments arranged by size: 2864 1306 411 398 274 147 28 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2313 2343 1178 Size: 30 165 30 910 30 4263 Fragments arranged by size: 4263 910 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2645 1186 Size: 1459 3969 BseYI C'CCAG_C Cuts at: 2080 2565 4631 5127 2080 Size: 485 2066 496 2381 Fragments arranged by size: 2381 2066 496 485 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4855 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2878 3639 4667 1540 Size: 275 186 877 761 1028 2301 Fragments arranged by size: 2301 1028 877 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2151 2417 2637 3489 3574 4645 2151 Size: 266 220 852 85 1071 2934 Fragments arranged by size: 2934 1071 852 266 220 85 BsmI GAATG_Cn' Cuts at: 2325 2939 3032 2325 Size: 614 93 4721 Fragments arranged by size: 4721 614 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2151 2417 2448 2637 2747 3489 3574 Size: 399 361 266 31 189 110 742 85 Cuts at: 3574 4645 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 361 266 189 110 85 31 BspEI T'CCGG_A Cuts at: 2527 5091 2527 Size: 2564 2864 BspHI T'CATG_A Cuts at: 3317 5047 3317 Size: 1730 3698 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2415 2783 844 Size: 1571 368 3489 Fragments arranged by size: 3489 1571 368 BsrBI CCG'CTC Cuts at: 1394 1823 3315 1394 Size: 429 1492 3507 Fragments arranged by size: 3507 1492 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3923 4097 65 Size: 1183 2675 174 1396 Fragments arranged by size: 2675 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2431 4072 4259 1700 Size: 110 621 1641 187 2869 Fragments arranged by size: 2869 1641 621 187 110 BsrGI T'GTAC_A Cuts at: 96 2464 96 Size: 2368 3060 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5428 BssSI C'ACGA_G Cuts at: 3488 4500 3488 Size: 1012 4416 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5428 BstBI TT'CG_AA Cuts at: 4232 4232 Size: 5428 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5428 BstYI r'GATC_y Cuts at: 1972 2193 2524 2758 3125 3510 3527 4979 5065 Size: 221 331 234 367 385 17 1452 86 Cuts at: 5065 5077 5423 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 331 234 221 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3937 Fragments arranged by size: 3937 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4689 Fragments arranged by size: 4689 683 56 BtsI GCAGTG_nn' Cuts at: 2944 3738 3758 2944 Size: 794 20 4614 Fragments arranged by size: 4614 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5428 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5393 DraI TTT'AAA Cuts at: 2732 2732 Size: 5428 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2278 4435 809 Size: 507 962 2157 1802 Fragments arranged by size: 2157 1802 962 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2371 2433 2875 Size: 54 1425 50 275 18 541 62 442 Cuts at: 2875 3764 5393 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 541 442 275 62 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2875 1537 Size: 275 1063 4090 Fragments arranged by size: 4090 1063 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2241 3358 1353 Size: 888 1117 3423 Fragments arranged by size: 3423 1117 888 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4015 4389 4535 387 Size: 1064 138 2426 374 146 1280 Fragments arranged by size: 2426 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2223 2595 2610 2629 2717 3491 4088 Size: 393 591 372 15 19 88 774 597 Cuts at: 4088 4875 5143 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 774 597 591 393 372 268 88 19 15 EcoO109I rG'GnC_Cy Cuts at: 2444 2444 Size: 5428 EcoRI G'AATT_C Cuts at: 2737 3115 2737 Size: 378 5050 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2188 2188 Size: 5428 FspI TGC'GCA Cuts at: 3934 3934 Size: 5428 HaeII r_GCGC'y Cuts at: 1678 4575 1678 Size: 2897 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2313 2345 2516 2548 4069 Size: 32 135 32 741 32 171 32 1521 Cuts at: 4069 4101 4143 4175 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1521 741 171 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2772 3018 669 Size: 988 281 834 246 3079 Fragments arranged by size: 3079 988 834 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5428 HpaI GTT'AAC Cuts at: 3018 3018 Size: 5428 KpnI G_GTAC'C Cuts at: 2753 2753 Size: 5428 MfeI C'AATT_G Cuts at: 3027 3027 Size: 5428 MluI A'CGCG_T Cuts at: 5304 5304 Size: 5428 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4542 4726 1305 Size: 3237 184 2007 Fragments arranged by size: 3237 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2373 10 Size: 54 1425 343 541 3065 Fragments arranged by size: 3065 1425 541 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3386 3745 3904 518 Size: 648 2220 359 159 2042 Fragments arranged by size: 2220 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2015 2844 3521 4669 4914 1606 Size: 409 829 677 1148 245 2120 Fragments arranged by size: 2120 1148 829 677 409 245 NaeI GCC'GGC Cuts at: 2433 2433 Size: 5428 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3937 Fragments arranged by size: 3937 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5428 NgoMIV G'CCGG_C Cuts at: 2431 2431 Size: 5428 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5428 NotI GC'GGCC_GC Cuts at: 2875 2875 Size: 5428 NspI r_CATG'y Cuts at: 1132 1229 2786 4331 1132 Size: 97 1557 1545 2229 Fragments arranged by size: 2229 1557 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5428 PciI A'CATG_T Cuts at: 4327 4327 Size: 5428 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5428 PfoI T'CCnGG_A Cuts at: 2331 2331 Size: 5428 PmeI GTTT'AAAC Cuts at: 2732 2732 Size: 5428 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3497 3529 5010 5042 3497 Size: 32 1481 32 3883 Fragments arranged by size: 3883 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5428 PsiI TTA'TAA Cuts at: 2998 2998 Size: 5428 PspOMI G'GGCC_C Cuts at: 2444 2444 Size: 5428 PstI C_TGCA'G Cuts at: 830 2412 2780 830 Size: 1582 368 3478 Fragments arranged by size: 3478 1582 368 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5428 SalI G'TCGA_C Cuts at: 1936 2770 1936 Size: 834 4594 SbfI CC_TGCA'GG Cuts at: 2780 2780 Size: 5428 ScaI AGT'ACT Cuts at: 3676 5388 3676 Size: 1712 3716 SfcI C'TryA_G Cuts at: 826 1045 2408 2776 3911 4592 4783 826 Size: 219 1363 368 1135 681 191 1471 Fragments arranged by size: 1471 1363 1135 681 368 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5428 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5428 SmaI CCC'GGG Cuts at: 1872 2755 1872 Size: 883 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3532 4433 4695 820 Size: 197 926 1589 901 262 1553 Fragments arranged by size: 1589 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3219 493 Size: 2726 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5428 SphI G_CATG'C Cuts at: 1132 2786 1132 Size: 1654 3774 SspI AAT'ATT Cuts at: 5 52 3352 5 Size: 47 3300 2081 Fragments arranged by size: 3300 2081 47 StuI AGG'CCT Cuts at: 1517 2231 1517 Size: 714 4714 StyI C'CwwG_G Cuts at: 513 1065 2004 2867 513 Size: 552 939 863 3074 Fragments arranged by size: 3074 939 863 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2417 3455 3472 3625 3810 4255 325 Size: 1255 837 1038 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1038 837 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2464 2677 3674 Size: 274 80 33 51 1443 487 213 997 Cuts at: 3674 5386 96 Size: 1712 138 Fragments arranged by size: 1712 1443 997 487 274 213 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5388 5420 5388 Size: 32 5396 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5428 XbaI T'CTAG_A Cuts at: 2764 2764 Size: 5428 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5428 XmaI C'CCGG_G Cuts at: 1870 2753 1870 Size: 883 4545 XmnI GAAnn'nnTTC Cuts at: 3557 3557 Size: 5428 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3236 276 Size: 53 83 186 936 1702 2468 Fragments arranged by size: 2468 1702 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI