Restriction map for
pFN21AB9806
(Circular) (Six-base) MAPSORT of: pFN21AB9806.seq Check: 7217 from: 1 to: 5383
pFN21AB9806 5383 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3193 278
Size: 53 83 186 936 1657 2468
Fragments arranged by size:
2468 1657 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2726 1937
Size: 789 4594
Acc65I G'GTAC_C
Cuts at: 2704 2704
Size: 5383
AclI AA'CG_TT
Cuts at: 1563 3510 3883 1563
Size: 1947 373 3063
Fragments arranged by size:
3063 1947 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3446 4830 5098 1632
Size: 1814 1384 268 1917
Fragments arranged by size:
1917 1814 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2198 820
Size: 197 1181 4005
Fragments arranged by size:
4005 1181 197
AflIII A'CryG_T
Cuts at: 1203 4282 5259 1203
Size: 3079 977 1327
Fragments arranged by size:
3079 1327 977
AgeI A'CCGG_T
Cuts at: 4214 4214
Size: 5383
AhdI GACnn_n'nnGTC
Cuts at: 4112 4112
Size: 5383
AleI CACnn'nnGTG
Cuts at: 1166 2291 1166
Size: 1125 4258
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2309 1184
Size: 1125 4258
AlwNI CAG_nnn'CTG
Cuts at: 3126 4698 3126
Size: 1572 3811
ApaI G_GGCC'C
Cuts at: 2127 2127
Size: 5383
ApaLI G'TGCA_C
Cuts at: 3440 4596 3440
Size: 1156 4227
ApoI r'AATT_y
Cuts at: 1493 2692 2918 3070 5179 1493
Size: 1199 226 152 2109 1697
Fragments arranged by size:
2109 1697 1199 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3937 160
Size: 872 2905 1606
Fragments arranged by size:
2905 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5383
AvaI C'yCGr_G
Cuts at: 1870 1943 2708 1870
Size: 73 765 4545
Fragments arranged by size:
4545 765 73
BamHI G'GATC_C
Cuts at: 2713 3080 2713
Size: 367 5016
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2041 2093 2285 2384 2704
Size: 325 217 627 254 52 192 99 320
Cuts at: 2704 4159 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 325 320 254 217 192
99 52
BanII G_rGCy'C
Cuts at: 1391 2127 2525 2702 1391
Size: 736 398 177 4072
Fragments arranged by size:
4072 736 398 177
BbeI G_GCGC'C
Cuts at: 2045 2388 2045
Size: 343 5040
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2471 928
Size: 971 572 3840
Fragments arranged by size:
3840 971 572
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3574 3608 1903
Size: 34 1637 34 3678
Fragments arranged by size:
3678 1637 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3275 4485 3275
Size: 1210 4173
BclI T'GATC_A
Cuts at: 1548 2142 1548
Size: 594 4789
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2039 2213 2378
Size: 107 122 71 631 605 367 174 165
Cuts at: 2378 2435 2654 2663 3994 136
Size: 57 219 9 1331 1525
Fragments arranged by size:
1525 1331 631 605 367 219 174 165
122 107 71 57 9
BglII A'GATC_T
Cuts at: 5378 5378
Size: 5383
BlpI GC'TnA_GC
Cuts at: 2106 2622 2800 2106
Size: 516 178 4689
Fragments arranged by size:
4689 516 178
Bme1580I G_kGCm'C
Cuts at: 1790 2127 2288 2309 3444 4600 1790
Size: 337 161 21 1135 1156 2573
Fragments arranged by size:
2573 1156 1135 337 161 21
BmgBI CAC'GTC
Cuts at: 1318 2230 1318
Size: 912 4471
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2123 3114 4072
Size: 416 53 83 399 24 706 991 958
Cuts at: 4072 442
Size: 1753
Fragments arranged by size:
1753 991 958 706 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5383
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4043 1239
Size: 2804 2579
Bpu10I CC'TnA_GC
Cuts at: 2209 2209
Size: 5383
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2249 3508 4373 4671 2249
Size: 1259 865 298 2961
Fragments arranged by size:
2961 1259 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4046 882
Size: 617 2547 2219
Fragments arranged by size:
2547 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3174 493
Size: 711 1970 2702
Fragments arranged by size:
2702 1970 711
BsaBI GATnn'nnATC
Cuts at: 5377 5377
Size: 5383
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2042 2385 2502 3190
Size: 53 83 186 936 509 343 117 688
Cuts at: 3190 3572 4207 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 688 635 509 382 343 186
117 83 53
BsaWI w'CCGG_w
Cuts at: 3816 4214 4488 4635 5046 3816
Size: 398 274 147 411 4153
Fragments arranged by size:
4153 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5158
Fragments arranged by size:
5158 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5383
BseYI C'CCAG_C
Cuts at: 2257 4586 5082 2257
Size: 2329 496 2558
Fragments arranged by size:
2558 2329 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2315 1163
Size: 573 579 4231
Fragments arranged by size:
4231 579 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2217 2833 3594 4622 1540
Size: 275 186 216 616 761 1028 2301
Fragments arranged by size:
2301 1028 761 616 275 216 186
BsiHKAI G_wGCw'C
Cuts at: 3444 3529 4600 3444
Size: 85 1071 4227
Fragments arranged by size:
4227 1071 85
BsmI GAATG_Cn'
Cuts at: 2894 2987 2894
Size: 93 5290
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2288 2309 2525 2702 3444 3529
Size: 399 337 161 21 216 177 742 85
Cuts at: 3529 4600 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 337 216 177 161
85 21
BspEI T'CCGG_A
Cuts at: 5046 5046
Size: 5383
BspHI T'CATG_A
Cuts at: 3272 5002 3272
Size: 1730 3653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2738 844
Size: 1894 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2018 3270 1394
Size: 429 195 1252 3507
Fragments arranged by size:
3507 1252 429 195
BsrDI GCAATG_nn'
Cuts at: 65 1248 2182 3878 4052 65
Size: 1183 934 1696 174 1396
Fragments arranged by size:
1696 1396 1183 934 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2044 2090 2096 2216 4027 4214 1700
Size: 110 234 46 6 120 1811 187 2869
Fragments arranged by size:
2869 1811 234 187 120 110 46 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5383
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5383
BssSI C'ACGA_G
Cuts at: 3443 4455 3443
Size: 1012 4371
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5383
BstBI TT'CG_AA
Cuts at: 4187 4187
Size: 5383
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5383
BstXI CCAn_nnnn'nTGG
Cuts at: 2155 2155
Size: 5383
BstYI r'GATC_y
Cuts at: 1972 2713 3080 3465 3482 4934 5020 5032 5378
Size: 741 367 385 17 1452 86 12 346
Cuts at: 5378 1972
Size: 1977
Fragments arranged by size:
1977 1452 741 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2406 2490 513
Size: 552 1341 84 3406
Fragments arranged by size:
3406 1341 552 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4644
Fragments arranged by size:
4644 683 56
BtsI GCAGTG_nn'
Cuts at: 2463 2899 3693 3713 2463
Size: 436 794 20 4133
Fragments arranged by size:
4133 794 436 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5383
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5348
DraI TTT'AAA
Cuts at: 2329 2687 2329
Size: 358 5025
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4390 809
Size: 507 3074 1802
Fragments arranged by size:
3074 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2214 2830 3719
Size: 54 1425 50 275 18 384 616 889
Cuts at: 3719 5348 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 616 384 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2214 2830 1537
Size: 275 402 616 4090
Fragments arranged by size:
4090 616 402 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2513 3313 1353
Size: 1160 800 3423
Fragments arranged by size:
3423 1160 800
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3970 4344 4490 387
Size: 1064 138 2381 374 146 1280
Fragments arranged by size:
2381 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3446 4043 4830 5098 1239
Size: 393 1814 597 787 268 1524
Fragments arranged by size:
1814 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2201 2201
Size: 5383
EcoO109I rG'GnC_Cy
Cuts at: 2123 2124 2123
Size: 1 5382
EcoRI G'AATT_C
Cuts at: 2692 3070 2692
Size: 378 5005
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 5383
FspI TGC'GCA
Cuts at: 3889 3889
Size: 5383
HaeII r_GCGC'y
Cuts at: 1678 2045 2103 2388 4530 1678
Size: 367 58 285 2142 2531
Fragments arranged by size:
2531 2142 367 285 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4024 4056 4098 4130 1373
Size: 32 135 32 2452 32 42 32 2626
Fragments arranged by size:
2626 2452 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2727 2973 669
Size: 988 281 789 246 3079
Fragments arranged by size:
3079 988 789 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5383
HpaI GTT'AAC
Cuts at: 2973 2973
Size: 5383
KasI G'GCGC_C
Cuts at: 2041 2384 2041
Size: 343 5040
KpnI G_GTAC'C
Cuts at: 2708 2708
Size: 5383
MfeI C'AATT_G
Cuts at: 2982 2982
Size: 5383
MluI A'CGCG_T
Cuts at: 5259 5259
Size: 5383
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 4497 4681 1305
Size: 684 2508 184 2007
Fragments arranged by size:
2508 2007 684 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3561
Fragments arranged by size:
3561 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2291 3341 3700 3859 518
Size: 648 1125 1050 359 159 2042
Fragments arranged by size:
2042 1125 1050 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2408 2492 2543 2799 3476 4624 4869 1606
Size: 802 84 51 256 677 1148 245 2120
Fragments arranged by size:
2120 1148 802 677 256 245 84 51
NaeI GCC'GGC
Cuts at: 2098 2098
Size: 5383
NarI GG'CG_CC
Cuts at: 2042 2385 2042
Size: 343 5040
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4831
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5383
NgoMIV G'CCGG_C
Cuts at: 2096 2096
Size: 5383
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5383
NotI GC'GGCC_GC
Cuts at: 2830 2830
Size: 5383
NspI r_CATG'y
Cuts at: 1132 1229 2741 4286 1132
Size: 97 1512 1545 2229
Fragments arranged by size:
2229 1545 1512 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5383
PciI A'CATG_T
Cuts at: 4282 4282
Size: 5383
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5383
PmeI GTTT'AAAC
Cuts at: 2687 2687
Size: 5383
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3452 3484 4965 4997 3452
Size: 32 1481 32 3838
Fragments arranged by size:
3838 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5383
PsiI TTA'TAA
Cuts at: 2953 2953
Size: 5383
PspOMI G'GGCC_C
Cuts at: 2123 2123
Size: 5383
PstI C_TGCA'G
Cuts at: 830 2443 2735 830
Size: 1613 292 3478
Fragments arranged by size:
3478 1613 292
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5383
SacII CC_GC'GG
Cuts at: 2409 2493 2409
Size: 84 5299
SalI G'TCGA_C
Cuts at: 1936 2725 1936
Size: 789 4594
SapI GCTCTTCn'nnn_
Cuts at: 2513 2513
Size: 5383
SbfI CC_TGCA'GG
Cuts at: 2735 2735
Size: 5383
ScaI AGT'ACT
Cuts at: 3631 5343 3631
Size: 1712 3671
SfcI C'TryA_G
Cuts at: 826 1045 2439 2731 3866 4547 4738 826
Size: 219 1394 292 1135 681 191 1471
Fragments arranged by size:
1471 1394 1135 681 292 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2213 2213
Size: 5383
SfoI GGC'GCC
Cuts at: 2043 2386 2043
Size: 343 5040
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5383
SgrAI Cr'CCGG_yG
Cuts at: 1700 2044 1700
Size: 344 5039
SmaI CCC'GGG
Cuts at: 1872 2710 1872
Size: 838 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2198 2264 3487 4388 4650 820
Size: 197 926 255 66 1223 901 262 1553
Fragments arranged by size:
1553 1223 926 901 262 255 197 66
SnaBI TAC'GTA
Cuts at: 493 3174 493
Size: 2681 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5383
SphI G_CATG'C
Cuts at: 1132 2741 1132
Size: 1609 3774
SspI AAT'ATT
Cuts at: 5 52 3307 5
Size: 47 3255 2081
Fragments arranged by size:
3255 2081 47
StuI AGG'CCT
Cuts at: 1517 2207 1517
Size: 690 4693
StyI C'CwwG_G
Cuts at: 513 1065 2148 2301 2535 2583 2822 513
Size: 552 1083 153 234 48 239 3074
Fragments arranged by size:
3074 1083 552 239 234 153 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3410 3427 3580 3765 4210 325
Size: 1255 1830 17 153 185 445 1498
Fragments arranged by size:
1830 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3629 5341 96
Size: 274 80 33 51 1443 1652 1712 138
Fragments arranged by size:
1712 1652 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5343 5375 5343
Size: 32 5351
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5383
XbaI T'CTAG_A
Cuts at: 2719 2719
Size: 5383
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5383
XmaI C'CCGG_G
Cuts at: 1870 2708 1870
Size: 838 4545
XmnI GAAnn'nnTTC
Cuts at: 3512 3512
Size: 5383
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3191 276
Size: 53 83 186 936 1657 2468
Fragments arranged by size:
2468 1657 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI PvuII RsrII SacI SanDI SexAI SrfI
SwaI XcmI