(Circular) (Six-base) MAPSORT of: pFN21AB9804.seq Check: 6963 from: 1 to: 5374 pFN21AB9804 5374 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3184 278 Size: 53 83 186 936 1648 2468 Fragments arranged by size: 2468 1648 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2717 1937 Size: 780 4594 Acc65I G'GTAC_C Cuts at: 2695 2695 Size: 5374 AclI AA'CG_TT Cuts at: 1563 3501 3874 1563 Size: 1938 373 3063 Fragments arranged by size: 3063 1938 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3437 4821 5089 1632 Size: 1805 1384 268 1917 Fragments arranged by size: 1917 1805 1384 268 AfeI AGC'GCT Cuts at: 2167 2167 Size: 5374 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5177 AflIII A'CryG_T Cuts at: 1203 2671 4273 5250 1203 Size: 1468 1602 977 1327 Fragments arranged by size: 1602 1468 1327 977 AgeI A'CCGG_T Cuts at: 4205 4205 Size: 5374 AhdI GACnn_n'nnGTC Cuts at: 4103 4103 Size: 5374 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5374 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5374 AlwNI CAG_nnn'CTG Cuts at: 3117 4689 3117 Size: 1572 3802 ApaI G_GGCC'C Cuts at: 2313 2515 2313 Size: 202 5172 ApaLI G'TGCA_C Cuts at: 3431 4587 3431 Size: 1156 4218 ApoI r'AATT_y Cuts at: 1493 2545 2683 2909 3061 5170 1493 Size: 1052 138 226 152 2109 1697 Fragments arranged by size: 2109 1697 1052 226 152 138 AseI AT'TA_AT Cuts at: 160 1032 3928 160 Size: 872 2896 1606 Fragments arranged by size: 2896 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 AvaI C'yCGr_G Cuts at: 1870 1943 2516 2699 1870 Size: 73 573 183 4545 Fragments arranged by size: 4545 573 183 73 BamHI G'GATC_C Cuts at: 2704 3071 2704 Size: 367 5007 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2421 2579 2695 4150 618 Size: 325 217 627 634 158 116 1455 1842 Fragments arranged by size: 1842 1455 634 627 325 217 158 116 BanII G_rGCy'C Cuts at: 1391 2264 2313 2515 2693 1391 Size: 873 49 202 178 4072 Fragments arranged by size: 4072 873 202 178 49 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2306 928 Size: 971 407 3996 Fragments arranged by size: 3996 971 407 BbvCI CC'TCA_GC Cuts at: 2507 2507 Size: 5374 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2141 2175 3565 3599 1903 Size: 34 50 34 120 34 1390 34 3678 Fragments arranged by size: 3678 1390 120 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3266 4476 3266 Size: 1210 4164 BclI T'GATC_A Cuts at: 1548 1548 Size: 5374 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2375 2384 3985 Size: 107 122 71 631 605 703 9 1601 Cuts at: 3985 136 Size: 1525 Fragments arranged by size: 1601 1525 703 631 605 122 107 71 9 BglII A'GATC_T Cuts at: 5369 5369 Size: 5374 BlpI GC'TnA_GC Cuts at: 2791 2791 Size: 5374 Bme1580I G_kGCm'C Cuts at: 1790 2313 2515 3435 4591 1790 Size: 523 202 920 1156 2573 Fragments arranged by size: 2573 1156 920 523 202 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5374 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3105 4063 442 Size: 416 53 83 399 24 1688 958 1753 Fragments arranged by size: 1753 1688 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5374 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2230 2230 Size: 5374 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4034 1239 Size: 2795 2579 Bpu10I CC'TnA_GC Cuts at: 2507 2507 Size: 5374 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3499 4364 4662 3499 Size: 865 298 4211 Fragments arranged by size: 4211 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2225 4037 882 Size: 617 726 1812 2219 Fragments arranged by size: 2219 1812 726 617 BsaAI yAC'GTr Cuts at: 493 1204 2373 3165 493 Size: 711 1169 792 2702 Fragments arranged by size: 2702 1169 792 711 BsaBI GATnn'nnATC Cuts at: 5368 5368 Size: 5374 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3181 3563 4198 275 Size: 53 83 186 936 1648 382 635 1451 Fragments arranged by size: 1648 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3807 4205 4479 4626 5037 3807 Size: 398 274 147 411 4144 Fragments arranged by size: 4144 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5149 Fragments arranged by size: 5149 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2041 2516 2534 1186 Size: 855 475 18 4026 Fragments arranged by size: 4026 855 475 18 BseYI C'CCAG_C Cuts at: 2556 4577 5073 2556 Size: 2021 496 2857 Fragments arranged by size: 2857 2021 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2255 2377 1163 Size: 573 519 122 4160 Fragments arranged by size: 4160 573 519 122 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2824 3585 4613 1540 Size: 275 186 823 761 1028 2301 Fragments arranged by size: 2301 1028 823 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3435 3520 4591 3435 Size: 85 1071 4218 Fragments arranged by size: 4218 1071 85 BsmI GAATG_Cn' Cuts at: 2885 2978 2885 Size: 93 5281 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2264 2313 2515 2693 3435 3520 4591 Size: 399 474 49 202 178 742 85 1071 Cuts at: 4591 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 474 399 202 178 85 49 BspEI T'CCGG_A Cuts at: 5037 5037 Size: 5374 BspHI T'CATG_A Cuts at: 3263 4993 3263 Size: 1730 3644 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2729 844 Size: 1885 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2267 3261 1394 Size: 429 444 994 3507 Fragments arranged by size: 3507 994 444 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2182 3869 4043 65 Size: 1183 934 1687 174 1396 Fragments arranged by size: 1687 1396 1183 934 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4018 4205 1700 Size: 110 2208 187 2869 Fragments arranged by size: 2869 2208 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5374 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5374 BssSI C'ACGA_G Cuts at: 2242 3434 4446 2242 Size: 1192 1012 3170 Fragments arranged by size: 3170 1192 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5374 BstBI TT'CG_AA Cuts at: 4178 4178 Size: 5374 BstEII G'GTnAC_C Cuts at: 1184 2471 1184 Size: 1287 4087 BstXI CCAn_nnnn'nTGG Cuts at: 2342 2342 Size: 5374 BstYI r'GATC_y Cuts at: 1972 2328 2704 3071 3456 3473 4925 5011 5023 Size: 356 376 367 385 17 1452 86 12 Cuts at: 5023 5369 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 376 367 356 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2217 2626 513 Size: 552 1152 409 3261 Fragments arranged by size: 3261 1152 552 409 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4635 Fragments arranged by size: 4635 683 56 BtsI GCAGTG_nn' Cuts at: 2890 3684 3704 2890 Size: 794 20 4560 Fragments arranged by size: 4560 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5374 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5339 DraI TTT'AAA Cuts at: 2678 2678 Size: 5374 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4381 809 Size: 507 3065 1802 Fragments arranged by size: 3065 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2605 2629 2821 Size: 54 1425 50 275 18 775 24 192 Cuts at: 2821 3710 5339 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 775 275 192 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2821 1537 Size: 275 1009 4090 Fragments arranged by size: 4090 1009 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2130 2199 3304 1353 Size: 777 69 1105 3423 Fragments arranged by size: 3423 1105 777 69 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3961 4335 4481 387 Size: 1064 138 2372 374 146 1280 Fragments arranged by size: 2372 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3437 4034 4821 5089 1239 Size: 393 1805 597 787 268 1524 Fragments arranged by size: 1805 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2519 2519 Size: 5374 EcoO109I rG'GnC_Cy Cuts at: 2310 2511 2512 2310 Size: 201 1 5172 Fragments arranged by size: 5172 201 1 EcoRI G'AATT_C Cuts at: 2683 3061 2683 Size: 378 4996 FspI TGC'GCA Cuts at: 3880 3880 Size: 5374 HaeII r_GCGC'y Cuts at: 1678 2169 4521 1678 Size: 491 2352 2531 Fragments arranged by size: 2531 2352 491 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2039 2071 4015 4047 4089 Size: 32 135 32 467 32 1944 32 42 Cuts at: 4089 4121 1373 Size: 32 2626 Fragments arranged by size: 2626 1944 467 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2718 2964 669 Size: 988 281 780 246 3079 Fragments arranged by size: 3079 988 780 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5374 HpaI GTT'AAC Cuts at: 2964 2964 Size: 5374 KpnI G_GTAC'C Cuts at: 2699 2699 Size: 5374 MfeI C'AATT_G Cuts at: 2973 2973 Size: 5374 MluI A'CGCG_T Cuts at: 5250 5250 Size: 5374 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4488 4672 1305 Size: 3183 184 2007 Fragments arranged by size: 3183 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2607 2631 10 Size: 54 1425 343 775 24 2753 Fragments arranged by size: 2753 1425 775 343 54 24 MslI CAynn'nnrTG Cuts at: 518 1166 3332 3691 3850 518 Size: 648 2166 359 159 2042 Fragments arranged by size: 2166 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2381 2446 2560 2790 3467 4615 4860 1606 Size: 775 65 114 230 677 1148 245 2120 Fragments arranged by size: 2120 1148 775 677 245 230 114 65 NcoI C'CATG_G Cuts at: 513 1065 2626 513 Size: 552 1561 3261 Fragments arranged by size: 3261 1561 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5374 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5374 NotI GC'GGCC_GC Cuts at: 2821 2821 Size: 5374 NspI r_CATG'y Cuts at: 1132 1229 2732 4277 1132 Size: 97 1503 1545 2229 Fragments arranged by size: 2229 1545 1503 97 PasI CC'CwG_GG Cuts at: 1348 2413 1348 Size: 1065 4309 PciI A'CATG_T Cuts at: 4273 4273 Size: 5374 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 4472 PmeI GTTT'AAAC Cuts at: 2678 2678 Size: 5374 PmlI CAC'GTG Cuts at: 2373 2373 Size: 5374 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3443 3475 4956 4988 3443 Size: 32 1481 32 3829 Fragments arranged by size: 3829 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5374 PsiI TTA'TAA Cuts at: 2944 2944 Size: 5374 PspOMI G'GGCC_C Cuts at: 2309 2511 2309 Size: 202 5172 PspXI vC'TCGA_Gb Cuts at: 2516 2516 Size: 5374 PstI C_TGCA'G Cuts at: 830 2445 2726 830 Size: 1615 281 3478 Fragments arranged by size: 3478 1615 281 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5374 SalI G'TCGA_C Cuts at: 1936 2716 1936 Size: 780 4594 SbfI CC_TGCA'GG Cuts at: 2726 2726 Size: 5374 ScaI AGT'ACT Cuts at: 3622 5334 3622 Size: 1712 3662 SfcI C'TryA_G Cuts at: 826 1045 2441 2722 3857 4538 4729 826 Size: 219 1396 281 1135 681 191 1471 Fragments arranged by size: 1471 1396 1135 681 281 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2375 2375 Size: 5374 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5374 SmaI CCC'GGG Cuts at: 1872 2701 1872 Size: 829 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2516 3478 4379 4641 820 Size: 197 926 573 962 901 262 1553 Fragments arranged by size: 1553 962 926 901 573 262 197 SnaBI TAC'GTA Cuts at: 493 3165 493 Size: 2672 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5374 SphI G_CATG'C Cuts at: 1132 2732 1132 Size: 1600 3774 SspI AAT'ATT Cuts at: 5 52 3298 5 Size: 47 3246 2081 Fragments arranged by size: 3246 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5374 StyI C'CwwG_G Cuts at: 513 1065 2397 2484 2626 2813 513 Size: 552 1332 87 142 187 3074 Fragments arranged by size: 3074 1332 552 187 142 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2426 2570 2649 3401 3418 3571 3756 Size: 1255 846 144 79 752 17 153 185 Cuts at: 3756 4201 325 Size: 445 1498 Fragments arranged by size: 1498 1255 846 752 445 185 153 144 79 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3620 5332 96 Size: 274 80 33 51 1443 1643 1712 138 Fragments arranged by size: 1712 1643 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2264 2296 2465 2497 5334 5366 2264 Size: 32 169 32 2837 32 2272 Fragments arranged by size: 2837 2272 169 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5374 XbaI T'CTAG_A Cuts at: 2710 2710 Size: 5374 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2192 2339 2624 2192 Size: 147 285 4942 Fragments arranged by size: 4942 285 147 XhoI C'TCGA_G Cuts at: 1943 2516 1943 Size: 573 4801 XmaI C'CCGG_G Cuts at: 1870 2699 1870 Size: 829 4545 XmnI GAAnn'nnTTC Cuts at: 3503 3503 Size: 5374 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3182 276 Size: 53 83 186 936 1648 2468 Fragments arranged by size: 2468 1648 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI