Restriction map for
pFN21AB9803
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BanI BbeI BbrPI BbvCI BbvI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsoBI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I MaeI MaeIII
MamI MfeI MluI MluNI MroI
MscI MseI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI Tru9I TseI
Tsp45I Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 281 CGCG CG^CG
AfaI 505 GTAC GT^AC
AluI 455 AGCT AG^CT
AvaII 212 GGTCC G^G[AT]CC
BamHI 510 GGATCC G^GATCC
BanII 443 GGGCTC G[GA]GC[CT]^C
BbsI 120 GAAGAC GAAGAC(2/6)
BpuAI 120 GAAGAC GAAGAC(2/6)
BsiYI 276 CCAGCCGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 276 CCAGCCGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 127 GAATGC GAATGC(1/-1)
Bsp1407I 504 TGTACA T^GTACA
BspMI 209 GCAGGT ACCTGC(4/8)
BsrGI 504 TGTACA T^GTACA
BsrI 177 ACTGG ACTGG(1/-1)
BstUI 281 CGCG CG^CG
BstYI 510 GGATCC [GA]^GATC[CT]
CfoI 297 GCGC GCG^C
DpnII 511 GATC ^GATC
DsaI 280 CCGCGG C^C[GA][CT]GG
EcoRV 586 GATATC GAT^ATC
FauI 282 GCGGG CCCGC(4/6)
HaeII 296 AGCGCC [GA]GCGC^[CT]
HhaI 297 GCGC GCG^C
HinP1I 297 GCGC G^CGC
KspI 280 CCGCGG CCGC^GG
MaeII 110 ACGT A^CGT
MboI 511 GATC ^GATC
MflI 510 GGATCC [GA]^GATC[CT]
MspA1I 280 CCGCGG C[AC]G^C[GT]G
MvnI 281 CGCG CG^CG
NdeII 511 GATC ^GATC
PleI 370 GACTC GAGTC(4/5)
RsaI 505 GTAC GT^AC
SacII 280 CCGCGG CCGC^GG
Sau3AI 511 GATC ^GATC
SmlI 446 CTCAAG C^T[CT][GA]AG
SspBI 504 TGTACA T^GTACA
TfiI 358 GATTC G^A[AT]TC
TspRI 405 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 510 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 511 GATCC GGATC(4/5)
510 GGATC
BcnI 300 CCCGG CC^[GC]GG
320 CCCGG
BglI 253 GCCTCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
569 GCCAGAGGGGC
BmyI 316 GTGCCC G[AGT]GC[ACT]^C
443 GGGCTC
BseRI 409 GAGGAG GAGGAG(10/8)
451 GAGGAG
Bsp1286I 316 GTGCCC G[AGT]GC[ACT]^C
443 GGGCTC
BstF5I 147 GGATG GGATG(2/0)
491 GGATG
EcoT14I 365 CCAAGG C^C[AT][AT]GG
523 CCAAGG
Fnu4HI 279 GCCGC GC^[ACGT]GC
286 GCCGC
FokI 147 GGATG GGATG(9/13)
491 GGATG
HaeIII 285 GGCC GG^CC
568 GGCC
HapII 301 CCGG C^CGG
321 CCGG
HinfI 358 GATTC G^A[ACGT]TC
370 GACTC
HpaII 301 CCGG C^CGG
321 CCGG
HphI 163 GGTGA GGTGA(8/7)
220 GGTGA
ItaI 279 GCCGC GC^[ACGT]GC
286 GCCGC
MspI 301 CCGG C^CGG
321 CCGG
MwoI 253 GCCTCCCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
569 GCCAGAGGGGC
NciI 300 CCCGG CC^[GC]GG
320 CCCGG
NlaIV 510 GGATCC GG[ACGT]^[ACGT]CC
566 GGGGCC
SfaNI 492 GATGC GCATC(5/9)
396 GCATC
StyI 365 CCAAGG C^C[AT][AT]GG
523 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AciI BsaJI BsmFI BssKI BstNI
Cac8I Cfr13I DdeI EcoRII MboII
MnlI MvaI Sau96I ScrFI