################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## Acc65I AccI AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII BanI BbeI BbrPI BcgI BciVI BfrI BglI BglII BpmI BsaAI BsgI BsiWI BsmI BspDI BspHI BspLU11I BsrDI Bst1107I BstBI BstXI BstZ17I Bsu36I ClaI DraI DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoRI EcoT22I FseI FspI HindIII HpaI KasI KpnI Ksp632I MluI MslI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI StuI SwaI XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 65 enzymes) # ################################### AatII 243 GACGTC GACGT^C AccIII 494 TCCGGA T^CCGGA AcyI 243 GACGTC G[GA]^CG[CT]C ApaI 225 GGGCCC GGGCC^C AspHI 513 GAGCAC G[AT]GC[AT]^C AspI 358 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 75 CCTAGG C^CTAGG BalI 551 TGGCCA TGG^CCA BamHI 425 GGATCC G^GATCC BbsI 737 GAAGAC GAAGAC(2/6) BbvCI 1069 GCTGAGG CCTCAGC(-5/-2) BclI 782 TGATCA T^GATCA BfaI 76 CTAG C^TAG BlnI 75 CCTAGG C^CTAGG BlpI 123 GCTGAGC GC^T[ACGT]AGC Bpu1102I 123 GCTGAGC GC^T[ACGT]AGC BpuAI 737 GAAGAC GAAGAC(2/6) BsaHI 243 GACGTC G[GA]^CG[CT]C BsaI 1265 GAGACC GGTCTC(1/5) BseAI 494 TCCGGA T^CCGGA BseRI 765 GAGGAG GAGGAG(10/8) BsiEI 210 CGGCCG CG[GA][CT]^CG BsiHKAI 513 GAGCAC G[AT]GC[AT]^C BsmBI 363 CGTCTC CGTCTC(1/5) Bsp1407I 437 TGTACA T^GTACA BspEI 494 TCCGGA T^CCGGA BspMI 656 ACCTGC ACCTGC(4/8) BsrBI 613 GAGCGG CCGCTC(-3/-3) BsrGI 437 TGTACA T^GTACA BsrI 1041 CCAGT ACTGG(1/-1) BssHII 273 GCGCGC G^CGCGC BstEII 1240 GGTGACC G^GT[ACGT]ACC BstPI 1240 GGTGACC G^GT[ACGT]ACC CelII 123 GCTGAGC GC^T[ACGT]AGC CpoI 260 CGGTCCG CG^G[AT]CCG DraIII 327 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 210 CGGCCG C^GGCCG EclXI 210 CGGCCG C^GGCCG Eco52I 210 CGGCCG C^GGCCG EcoO65I 1240 GGTGACC G^GT[ACGT]ACC EcoRV 883 GATATC GAT^ATC FbaI 782 TGATCA T^GATCA HaeII 183 GGCGCT [GA]GCGC^[CT] Hin1I 243 GACGTC G[GA]^CG[CT]C HincII 355 GTTGAC GT[CT]^[GA]AC HindII 355 GTTGAC GT[CT]^[GA]AC MaeI 76 CTAG C^TAG MaeII 244 ACGT A^CGT MboII 737 GAAGA GAAGA(8/7) MfeI 1196 CAATTG C^AATTG MluNI 551 TGGCCA TGG^CCA MroI 494 TCCGGA T^CCGGA MscI 551 TGGCCA TGG^CCA MunI 1196 CAATTG C^AATTG NspI 1166 GCATGC [GA]CATG^[CT] PflFI 358 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC RsrII 260 CGGTCCG CG^G[AT]CCG SmaI 229 CCCGGG CCC^GGG SphI 1166 GCATGC GCATG^C SspBI 437 TGTACA T^GTACA TaqI 835 TCGA T^CGA Tth111I 358 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 835 TCGA T^CGA XmaCI 229 CCCGGG C^CCGGG XmaI 229 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AfaI 438 GTAC GT^AC 654 GTAC AvaI 34 CCCGAG C^[CT]CG[GA]G 229 CCCGGG BsaWI 320 TCCGGT [AT]^CCGG[AT] 494 TCCGGA BsoBI 34 CCCGAG C^[CT]CG[GA]G 229 CCCGGG BssSI 118 CTCGTG CACGAG(-5/-1) 676 CTCGTG EaeI 210 CGGCCG [CT]^GGCC[GA] 551 TGGCCA KspI 136 CCGCGG CCGC^GG 221 CCGCGG MseI 731 TTAA T^TAA 817 TTAA NcoI 59 CCATGG C^CATGG 1260 CCATGG PflMI 326 CCACCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1255 CCAACCCATGG PvuII 199 CAGCTG CAG^CTG 430 CAGCTG RsaI 438 GTAC GT^AC 654 GTAC SacII 136 CCGCGG CCGC^GG 221 CCGCGG SmlI 868 CTTGAG C^T[CT][GA]AG 1130 CTCAAG Tru9I 731 TTAA T^TAA 817 TTAA Van91I 326 CCACCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1255 CCAACCCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AccII AciI AcsI AluI AlwI AlwNI ApoI AvaII BanII BbvI BcnI BmyI BsaBI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MamI MboI MflI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PpuMI PstI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI TseI Tsp45I Tsp509I TspRI XhoII