(Circular) (Six-base) MAPSORT of: pFN21AB9794.seq Check: 7163 from: 1 to: 5356 pFN21AB9794 5356 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3166 278 Size: 53 83 186 936 1630 2468 Fragments arranged by size: 2468 1630 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2699 1937 Size: 762 4594 Acc65I G'GTAC_C Cuts at: 2677 2677 Size: 5356 AclI AA'CG_TT Cuts at: 1563 3483 3856 1563 Size: 1920 373 3063 Fragments arranged by size: 3063 1920 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3419 4803 5071 1632 Size: 1787 1384 268 1917 Fragments arranged by size: 1917 1787 1384 268 AfeI AGC'GCT Cuts at: 2034 2034 Size: 5356 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5159 AflIII A'CryG_T Cuts at: 1203 4255 5232 1203 Size: 3052 977 1327 Fragments arranged by size: 3052 1327 977 AgeI A'CCGG_T Cuts at: 4187 4187 Size: 5356 AhdI GACnn_n'nnGTC Cuts at: 4085 4085 Size: 5356 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5356 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5356 AlwNI CAG_nnn'CTG Cuts at: 3099 4671 3099 Size: 1572 3784 ApaLI G'TGCA_C Cuts at: 3413 4569 3413 Size: 1156 4200 ApoI r'AATT_y Cuts at: 1493 2665 2891 3043 5152 1493 Size: 1172 226 152 2109 1697 Fragments arranged by size: 2109 1697 1172 226 152 AscI GG'CGCG_CC Cuts at: 2160 2160 Size: 5356 AseI AT'TA_AT Cuts at: 160 1032 3910 160 Size: 872 2878 1606 Fragments arranged by size: 2878 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5356 AvaI C'yCGr_G Cuts at: 1870 1943 2125 2225 2243 2569 2681 1870 Size: 73 182 100 18 326 112 4545 Fragments arranged by size: 4545 326 182 112 100 73 18 BamHI G'GATC_C Cuts at: 2686 3053 2686 Size: 367 4989 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2072 2495 2677 4132 618 Size: 325 217 627 285 423 182 1455 1842 Fragments arranged by size: 1842 1455 627 423 325 285 217 182 BanII G_rGCy'C Cuts at: 1391 2049 2675 1391 Size: 658 626 4072 Fragments arranged by size: 4072 658 626 BbeI G_GCGC'C Cuts at: 2076 2076 Size: 5356 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2625 928 Size: 971 726 3659 Fragments arranged by size: 3659 971 726 BbvCI CC'TCA_GC Cuts at: 2649 2649 Size: 5356 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2521 2555 3547 3581 1903 Size: 34 584 34 992 34 3678 Fragments arranged by size: 3678 992 584 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3248 4458 3248 Size: 1210 4146 BclI T'GATC_A Cuts at: 1548 1548 Size: 5356 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2044 3967 136 Size: 107 122 71 631 605 372 1923 1525 Fragments arranged by size: 1923 1525 631 605 372 122 107 71 BglII A'GATC_T Cuts at: 5351 5351 Size: 5356 BlpI GC'TnA_GC Cuts at: 2773 2773 Size: 5356 Bme1580I G_kGCm'C Cuts at: 1790 2498 2544 3417 4573 1790 Size: 708 46 873 1156 2573 Fragments arranged by size: 2573 1156 873 708 46 BmgBI CAC'GTC Cuts at: 1318 2341 1318 Size: 1023 4333 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3087 4045 442 Size: 416 53 83 399 24 1670 958 1753 Fragments arranged by size: 1753 1670 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5356 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2101 2438 4016 1239 Size: 862 337 1578 2579 Fragments arranged by size: 2579 1578 862 337 Bpu10I CC'TnA_GC Cuts at: 2649 2649 Size: 5356 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3481 4346 4644 3481 Size: 865 298 4193 Fragments arranged by size: 4193 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4019 882 Size: 617 2520 2219 Fragments arranged by size: 2520 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3147 493 Size: 711 1943 2702 Fragments arranged by size: 2702 1943 711 BsaBI GATnn'nnATC Cuts at: 5350 5350 Size: 5356 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2073 3163 3545 4180 Size: 53 83 186 936 540 1090 382 635 Cuts at: 4180 275 Size: 1451 Fragments arranged by size: 1451 1090 936 635 540 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3789 4187 4461 4608 5019 3789 Size: 398 274 147 411 4126 Fragments arranged by size: 4126 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5131 Fragments arranged by size: 5131 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2477 1186 Size: 1291 4065 BseYI C'CCAG_C Cuts at: 2491 4559 5055 2491 Size: 2068 496 2792 Fragments arranged by size: 2792 2068 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2510 1163 Size: 573 774 4009 Fragments arranged by size: 4009 774 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2413 2806 3567 4595 1540 Size: 275 186 412 393 761 1028 2301 Fragments arranged by size: 2301 1028 761 412 393 275 186 BsiHKAI G_wGCw'C Cuts at: 3417 3502 4573 3417 Size: 85 1071 4200 Fragments arranged by size: 4200 1071 85 BsmI GAATG_Cn' Cuts at: 2867 2960 2867 Size: 93 5263 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2498 2544 2675 3417 3502 4573 Size: 399 259 449 46 131 742 85 1071 Cuts at: 4573 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 449 399 259 131 85 46 BspEI T'CCGG_A Cuts at: 5019 5019 Size: 5356 BspHI T'CATG_A Cuts at: 3245 4975 3245 Size: 1730 3626 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2184 2286 2458 2711 844 Size: 1340 102 172 253 3489 Fragments arranged by size: 3489 1340 253 172 102 BsrBI CCG'CTC Cuts at: 1394 1823 2085 3243 1394 Size: 429 262 1158 3507 Fragments arranged by size: 3507 1158 429 262 BsrDI GCAATG_nn' Cuts at: 65 1248 3851 4025 65 Size: 1183 2603 174 1396 Fragments arranged by size: 2603 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2412 2585 4000 4187 1700 Size: 110 602 173 1415 187 2869 Fragments arranged by size: 2869 1415 602 187 173 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5356 BssHII G'CGCG_C Cuts at: 1925 2160 1925 Size: 235 5121 BssSI C'ACGA_G Cuts at: 3416 4428 3416 Size: 1012 4344 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5356 BstBI TT'CG_AA Cuts at: 4160 4160 Size: 5356 BstEII G'GTnAC_C Cuts at: 1184 2470 1184 Size: 1286 4070 BstYI r'GATC_y Cuts at: 1972 2686 3053 3438 3455 4907 4993 5005 5351 Size: 714 367 385 17 1452 86 12 346 Cuts at: 5351 1972 Size: 1977 Fragments arranged by size: 1977 1452 714 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2132 513 Size: 552 939 128 3737 Fragments arranged by size: 3737 939 552 128 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4617 Fragments arranged by size: 4617 683 56 BtsI GCAGTG_nn' Cuts at: 2644 2872 3666 3686 2644 Size: 228 794 20 4314 Fragments arranged by size: 4314 794 228 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5356 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5321 DraI TTT'AAA Cuts at: 2660 2660 Size: 5356 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4363 809 Size: 507 3047 1802 Fragments arranged by size: 3047 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2451 2803 3692 Size: 54 1425 50 275 18 621 352 889 Cuts at: 3692 5321 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 621 352 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2803 1537 Size: 275 991 4090 Fragments arranged by size: 4090 991 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2049 3286 1353 Size: 696 1237 3423 Fragments arranged by size: 3423 1237 696 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2043 3943 4317 4463 387 Size: 1064 138 454 1900 374 146 1280 Fragments arranged by size: 1900 1280 1064 454 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2101 2438 3419 4016 4803 5071 1239 Size: 393 469 337 981 597 787 268 1524 Fragments arranged by size: 1524 981 787 597 469 393 337 268 EcoNI CCTnn'n_nnAGG Cuts at: 2428 2503 2428 Size: 75 5281 EcoO109I rG'GnC_Cy Cuts at: 2254 2275 2254 Size: 21 5335 EcoRI G'AATT_C Cuts at: 2665 3043 2665 Size: 378 4978 FspI TGC'GCA Cuts at: 3862 3862 Size: 5356 HaeII r_GCGC'y Cuts at: 1678 2036 2076 2124 4503 1678 Size: 358 40 48 2379 2531 Fragments arranged by size: 2531 2379 358 48 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3997 4029 4071 4103 1373 Size: 32 135 32 2425 32 42 32 2626 Fragments arranged by size: 2626 2425 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2700 2946 669 Size: 988 281 762 246 3079 Fragments arranged by size: 3079 988 762 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5356 HpaI GTT'AAC Cuts at: 2946 2946 Size: 5356 KasI G'GCGC_C Cuts at: 2072 2072 Size: 5356 KpnI G_GTAC'C Cuts at: 2681 2681 Size: 5356 MfeI C'AATT_G Cuts at: 2955 2955 Size: 5356 MluI A'CGCG_T Cuts at: 5232 5232 Size: 5356 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4470 4654 1305 Size: 3165 184 2007 Fragments arranged by size: 3165 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2453 10 Size: 54 1425 343 621 2913 Fragments arranged by size: 2913 1425 621 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3314 3673 3832 518 Size: 648 2148 359 159 2042 Fragments arranged by size: 2148 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2182 2198 2772 3449 4597 4842 1606 Size: 576 16 574 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 576 574 245 16 NaeI GCC'GGC Cuts at: 2587 2587 Size: 5356 NarI GG'CG_CC Cuts at: 2073 2073 Size: 5356 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3865 Fragments arranged by size: 3865 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5356 NgoMIV G'CCGG_C Cuts at: 2585 2585 Size: 5356 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5356 NotI GC'GGCC_GC Cuts at: 2803 2803 Size: 5356 NspI r_CATG'y Cuts at: 1132 1229 2311 2320 2714 4259 1132 Size: 97 1082 9 394 1545 2229 Fragments arranged by size: 2229 1545 1082 394 97 9 PasI CC'CwG_GG Cuts at: 1348 2505 1348 Size: 1157 4199 PciI A'CATG_T Cuts at: 4255 4255 Size: 5356 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5356 PmeI GTTT'AAAC Cuts at: 2660 2660 Size: 5356 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2528 2560 3425 3457 4938 4970 2528 Size: 32 865 32 1481 32 2914 Fragments arranged by size: 2914 1481 865 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2275 2275 Size: 5356 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5356 PsiI TTA'TAA Cuts at: 2926 2926 Size: 5356 PspXI vC'TCGA_Gb Cuts at: 2569 2569 Size: 5356 PstI C_TGCA'G Cuts at: 830 2708 830 Size: 1878 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5356 RsrII CG'GwC_CG Cuts at: 2613 2613 Size: 5356 SalI G'TCGA_C Cuts at: 1936 2698 1936 Size: 762 4594 SbfI CC_TGCA'GG Cuts at: 2708 2708 Size: 5356 ScaI AGT'ACT Cuts at: 3604 5316 3604 Size: 1712 3644 SfcI C'TryA_G Cuts at: 826 1045 2398 2704 3839 4520 4711 826 Size: 219 1353 306 1135 681 191 1471 Fragments arranged by size: 1471 1353 1135 681 306 219 191 SfoI GGC'GCC Cuts at: 2074 2074 Size: 5356 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5356 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5356 SmaI CCC'GGG Cuts at: 1872 2227 2683 1872 Size: 355 456 4545 Fragments arranged by size: 4545 456 355 SmlI C'TyrA_G Cuts at: 820 1017 1943 2569 3460 4361 4623 820 Size: 197 926 626 891 901 262 1553 Fragments arranged by size: 1553 926 901 891 626 262 197 SnaBI TAC'GTA Cuts at: 493 3147 493 Size: 2654 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5356 SphI G_CATG'C Cuts at: 1132 2311 2714 1132 Size: 1179 403 3774 Fragments arranged by size: 3774 1179 403 SspI AAT'ATT Cuts at: 5 52 3280 5 Size: 47 3228 2081 Fragments arranged by size: 3228 2081 47 StuI AGG'CCT Cuts at: 1517 2423 2647 1517 Size: 906 224 4226 Fragments arranged by size: 4226 906 224 StyI C'CwwG_G Cuts at: 513 1065 2004 2475 2795 513 Size: 552 939 471 320 3074 Fragments arranged by size: 3074 939 552 471 320 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2630 3383 3400 3553 3738 4183 325 Size: 1255 1050 753 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1050 753 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3602 5314 96 Size: 274 80 33 51 1443 1625 1712 138 Fragments arranged by size: 1712 1625 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5316 5348 5316 Size: 32 5324 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5356 XbaI T'CTAG_A Cuts at: 2692 2692 Size: 5356 XhoI C'TCGA_G Cuts at: 1943 2569 1943 Size: 626 4730 XmaI C'CCGG_G Cuts at: 1870 2225 2681 1870 Size: 355 456 4545 Fragments arranged by size: 4545 456 355 XmnI GAAnn'nnTTC Cuts at: 3485 3485 Size: 5356 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3164 276 Size: 53 83 186 936 1630 2468 Fragments arranged by size: 2468 1630 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI RsrII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PsrI PvuII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI