(Circular) (Six-base) MAPSORT of: pFN21AB9791.seq Check: 2596 from: 1 to: 5335 pFN21AB9791 5335 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2207 2453 2207 Size: 246 5089 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3145 278 Size: 53 83 186 936 1609 2468 Fragments arranged by size: 2468 1609 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2678 1937 Size: 741 4594 Acc65I G'GTAC_C Cuts at: 2656 2656 Size: 5335 AclI AA'CG_TT Cuts at: 1563 3462 3835 1563 Size: 1899 373 3063 Fragments arranged by size: 3063 1899 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3398 4782 5050 1632 Size: 1766 1384 268 1917 Fragments arranged by size: 1917 1766 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5138 AflIII A'CryG_T Cuts at: 1203 4234 5211 1203 Size: 3031 977 1327 Fragments arranged by size: 3031 1327 977 AgeI A'CCGG_T Cuts at: 4166 4166 Size: 5335 AhdI GACnn_n'nnGTC Cuts at: 4064 4064 Size: 5335 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5335 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5335 AlwNI CAG_nnn'CTG Cuts at: 2310 3078 4650 2310 Size: 768 1572 2995 Fragments arranged by size: 2995 1572 768 ApaI G_GGCC'C Cuts at: 2172 2172 Size: 5335 ApaLI G'TGCA_C Cuts at: 3392 4548 3392 Size: 1156 4179 ApoI r'AATT_y Cuts at: 1493 2644 2870 3022 5131 1493 Size: 1151 226 152 2109 1697 Fragments arranged by size: 2109 1697 1151 226 152 AseI AT'TA_AT Cuts at: 160 1032 3889 160 Size: 872 2857 1606 Fragments arranged by size: 2857 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5335 AvaI C'yCGr_G Cuts at: 1870 1943 2136 2455 2660 1870 Size: 73 193 319 205 4545 Fragments arranged by size: 4545 319 205 193 73 BamHI G'GATC_C Cuts at: 2665 3032 2665 Size: 367 4968 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2156 2219 2374 2656 4111 Size: 325 217 627 369 63 155 282 1455 Cuts at: 4111 618 Size: 1842 Fragments arranged by size: 1842 1455 627 369 325 282 217 155 63 BanII G_rGCy'C Cuts at: 1391 2172 2654 1391 Size: 781 482 4072 Fragments arranged by size: 4072 781 482 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4364 BbvCI CC'TCA_GC Cuts at: 2442 2615 2442 Size: 173 5162 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2360 2394 3526 3560 1903 Size: 34 50 34 339 34 1132 34 3678 Fragments arranged by size: 3678 1132 339 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3227 4437 3227 Size: 1210 4125 BclI T'GATC_A Cuts at: 1548 1548 Size: 5335 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2568 2625 3946 Size: 107 122 71 631 605 896 57 1321 Cuts at: 3946 136 Size: 1525 Fragments arranged by size: 1525 1321 896 631 605 122 107 71 57 BglII A'GATC_T Cuts at: 5330 5330 Size: 5335 BlpI GC'TnA_GC Cuts at: 2752 2752 Size: 5335 Bme1580I G_kGCm'C Cuts at: 1790 2161 2172 2224 3396 4552 1790 Size: 371 11 52 1172 1156 2573 Fragments arranged by size: 2573 1172 1156 371 52 11 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5335 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2052 2509 3066 Size: 416 53 83 399 24 635 457 557 Cuts at: 3066 4024 442 Size: 958 1753 Fragments arranged by size: 1753 958 635 557 457 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5335 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2210 3995 1239 Size: 971 1785 2579 Fragments arranged by size: 2579 1785 971 Bpu10I CC'TnA_GC Cuts at: 2442 2615 2442 Size: 173 5162 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3460 4325 4623 3460 Size: 865 298 4172 Fragments arranged by size: 4172 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3998 882 Size: 617 2499 2219 Fragments arranged by size: 2499 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3126 493 Size: 711 1922 2702 Fragments arranged by size: 2702 1922 711 BsaBI GATnn'nnATC Cuts at: 5329 5329 Size: 5335 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3142 3524 4159 275 Size: 53 83 186 936 1609 382 635 1451 Fragments arranged by size: 1609 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3768 4166 4440 4587 4998 3768 Size: 398 274 147 411 4105 Fragments arranged by size: 4105 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2114 2144 1178 Size: 30 165 30 711 30 4369 Fragments arranged by size: 4369 711 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 2042 2603 1186 Size: 844 12 561 3918 Fragments arranged by size: 3918 844 561 12 BseYI C'CCAG_C Cuts at: 2165 2195 2310 4538 5034 2165 Size: 30 115 2228 496 2466 Fragments arranged by size: 2466 2228 496 115 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2244 2590 1163 Size: 573 508 346 3908 Fragments arranged by size: 3908 573 508 346 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2785 3546 4574 1540 Size: 275 186 784 761 1028 2301 Fragments arranged by size: 2301 1028 784 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3396 3481 4552 3396 Size: 85 1071 4179 Fragments arranged by size: 4179 1071 85 BsmI GAATG_Cn' Cuts at: 2846 2939 2846 Size: 93 5242 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2161 2172 2224 2654 3396 3481 4552 Size: 399 371 11 52 430 742 85 1071 Cuts at: 4552 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 430 399 371 85 52 11 BspEI T'CCGG_A Cuts at: 4998 4998 Size: 5335 BspHI T'CATG_A Cuts at: 3224 4954 3224 Size: 1730 3605 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2207 2453 2690 844 Size: 1363 246 237 3489 Fragments arranged by size: 3489 1363 246 237 BsrBI CCG'CTC Cuts at: 1394 1823 3222 1394 Size: 429 1399 3507 Fragments arranged by size: 3507 1399 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2093 3830 4004 65 Size: 1183 845 1737 174 1396 Fragments arranged by size: 1737 1396 1183 845 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2238 3979 4166 1700 Size: 110 428 1741 187 2869 Fragments arranged by size: 2869 1741 428 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5335 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5335 BssSI C'ACGA_G Cuts at: 3395 4407 3395 Size: 1012 4323 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5335 BstBI TT'CG_AA Cuts at: 2584 4139 2584 Size: 1555 3780 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5335 BstYI r'GATC_y Cuts at: 1972 2665 3032 3417 3434 4886 4972 4984 5330 Size: 693 367 385 17 1452 86 12 346 Cuts at: 5330 1972 Size: 1977 Fragments arranged by size: 1977 1452 693 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4783 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2310 2590 487 Size: 683 56 1084 280 3232 Fragments arranged by size: 3232 1084 683 280 56 BtsI GCAGTG_nn' Cuts at: 2592 2851 3645 3665 2592 Size: 259 794 20 4262 Fragments arranged by size: 4262 794 259 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5335 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5300 DraI TTT'AAA Cuts at: 2639 2639 Size: 5335 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4342 809 Size: 507 3026 1802 Fragments arranged by size: 3026 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2226 2626 2782 Size: 54 1425 50 275 18 396 400 156 Cuts at: 2782 3671 5300 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 400 396 275 156 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2782 1537 Size: 275 970 4090 Fragments arranged by size: 4090 970 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2186 3265 1353 Size: 833 1079 3423 Fragments arranged by size: 3423 1079 833 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2390 3922 4296 4442 387 Size: 1064 138 801 1532 374 146 1280 Fragments arranged by size: 1532 1280 1064 801 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2210 3398 3995 4782 5050 1239 Size: 393 578 1188 597 787 268 1524 Fragments arranged by size: 1524 1188 787 597 578 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2189 2189 Size: 5335 EcoO109I rG'GnC_Cy Cuts at: 2122 2169 2199 2122 Size: 47 30 5258 Fragments arranged by size: 5258 47 30 EcoRI G'AATT_C Cuts at: 2644 3022 2644 Size: 378 4957 FspI TGC'GCA Cuts at: 3841 3841 Size: 5335 HaeII r_GCGC'y Cuts at: 1678 4482 1678 Size: 2804 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2114 2146 3976 4008 4050 Size: 32 135 32 542 32 1830 32 42 Cuts at: 4050 4082 1373 Size: 32 2626 Fragments arranged by size: 2626 1830 542 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2679 2925 669 Size: 988 281 741 246 3079 Fragments arranged by size: 3079 988 741 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5335 HpaI GTT'AAC Cuts at: 2925 2925 Size: 5335 KpnI G_GTAC'C Cuts at: 2660 2660 Size: 5335 MfeI C'AATT_G Cuts at: 2067 2934 2067 Size: 867 4468 MluI A'CGCG_T Cuts at: 5211 5211 Size: 5335 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4449 4633 1305 Size: 3144 184 2007 Fragments arranged by size: 3144 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3513 Fragments arranged by size: 3513 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3293 3652 3811 518 Size: 648 2127 359 159 2042 Fragments arranged by size: 2127 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2333 2384 2424 2549 2751 3428 4576 4821 Size: 727 51 40 125 202 677 1148 245 Cuts at: 4821 1606 Size: 2120 Fragments arranged by size: 2120 1148 727 677 245 202 125 51 40 NaeI GCC'GGC Cuts at: 2240 2240 Size: 5335 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4783 NdeI CA'TA_TG Cuts at: 387 387 Size: 5335 NgoMIV G'CCGG_C Cuts at: 2238 2238 Size: 5335 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5335 NotI GC'GGCC_GC Cuts at: 2782 2782 Size: 5335 NspI r_CATG'y Cuts at: 1132 1229 2693 4238 1132 Size: 97 1464 1545 2229 Fragments arranged by size: 2229 1545 1464 97 PasI CC'CwG_GG Cuts at: 1348 2557 1348 Size: 1209 4126 PciI A'CATG_T Cuts at: 4234 4234 Size: 5335 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5335 PmeI GTTT'AAAC Cuts at: 2639 2639 Size: 5335 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3404 3436 4917 4949 3404 Size: 32 1481 32 3790 Fragments arranged by size: 3790 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2122 2122 Size: 5335 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5335 PsiI TTA'TAA Cuts at: 2905 2905 Size: 5335 PspOMI G'GGCC_C Cuts at: 2168 2168 Size: 5335 PstI C_TGCA'G Cuts at: 830 2383 2687 830 Size: 1553 304 3478 Fragments arranged by size: 3478 1553 304 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5335 PvuII CAG'CTG Cuts at: 2384 2384 Size: 5335 RsrII CG'GwC_CG Cuts at: 2328 2328 Size: 5335 SalI G'TCGA_C Cuts at: 1936 2677 1936 Size: 741 4594 SapI GCTCTTCn'nnn_ Cuts at: 2186 2186 Size: 5335 SbfI CC_TGCA'GG Cuts at: 2687 2687 Size: 5335 ScaI AGT'ACT Cuts at: 2408 3583 5295 2408 Size: 1175 1712 2448 Fragments arranged by size: 2448 1712 1175 SfcI C'TryA_G Cuts at: 826 1045 2379 2683 3818 4499 4690 826 Size: 219 1334 304 1135 681 191 1471 Fragments arranged by size: 1471 1334 1135 681 304 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5335 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5335 SmaI CCC'GGG Cuts at: 1872 2457 2662 1872 Size: 585 205 4545 Fragments arranged by size: 4545 585 205 SmlI C'TyrA_G Cuts at: 820 1017 1943 3439 4340 4602 820 Size: 197 926 1496 901 262 1553 Fragments arranged by size: 1553 1496 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3126 493 Size: 2633 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5335 SphI G_CATG'C Cuts at: 1132 2693 1132 Size: 1561 3774 SspI AAT'ATT Cuts at: 5 52 3259 5 Size: 47 3207 2081 Fragments arranged by size: 3207 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5335 StyI C'CwwG_G Cuts at: 513 1065 2774 513 Size: 552 1709 3074 Fragments arranged by size: 3074 1709 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2302 3362 3379 3532 3717 4162 325 Size: 1255 722 1060 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1060 722 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2406 3581 5293 Size: 274 80 33 51 1443 429 1175 1712 Cuts at: 5293 96 Size: 138 Fragments arranged by size: 1712 1443 1175 429 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5295 5327 5295 Size: 32 5303 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5335 XbaI T'CTAG_A Cuts at: 2671 2671 Size: 5335 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5335 XmaI C'CCGG_G Cuts at: 1870 2455 2660 1870 Size: 585 205 4545 Fragments arranged by size: 4545 585 205 XmnI GAAnn'nnTTC Cuts at: 3464 3464 Size: 5335 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3143 276 Size: 53 83 186 936 1609 2468 Fragments arranged by size: 2468 1609 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI