(Circular) (Six-base) MAPSORT of: pFN21AB9789.seq Check: 1226 from: 1 to: 5311 pFN21AB9789 5311 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3121 278 Size: 53 83 186 936 1585 2468 Fragments arranged by size: 2468 1585 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2654 1937 Size: 717 4594 Acc65I G'GTAC_C Cuts at: 2632 2632 Size: 5311 AclI AA'CG_TT Cuts at: 1563 3438 3811 1563 Size: 1875 373 3063 Fragments arranged by size: 3063 1875 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3374 4758 5026 1632 Size: 1742 1384 268 1917 Fragments arranged by size: 1917 1742 1384 268 AfeI AGC'GCT Cuts at: 2412 2412 Size: 5311 AflII C'TTAA_G Cuts at: 820 1017 2414 820 Size: 197 1397 3717 Fragments arranged by size: 3717 1397 197 AflIII A'CryG_T Cuts at: 1203 4210 5187 1203 Size: 3007 977 1327 Fragments arranged by size: 3007 1327 977 AgeI A'CCGG_T Cuts at: 4142 4142 Size: 5311 AhdI GACnn_n'nnGTC Cuts at: 4040 4040 Size: 5311 AleI CACnn'nnGTG Cuts at: 1166 2293 1166 Size: 1127 4184 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5311 AlwNI CAG_nnn'CTG Cuts at: 2362 3054 4626 2362 Size: 692 1572 3047 Fragments arranged by size: 3047 1572 692 ApaI G_GGCC'C Cuts at: 2453 2529 2453 Size: 76 5235 ApaLI G'TGCA_C Cuts at: 3368 4524 3368 Size: 1156 4155 ApoI r'AATT_y Cuts at: 1493 2620 2846 2998 5107 1493 Size: 1127 226 152 2109 1697 Fragments arranged by size: 2109 1697 1127 226 152 AscI GG'CGCG_CC Cuts at: 2105 2281 2105 Size: 176 5135 AseI AT'TA_AT Cuts at: 160 1032 3865 160 Size: 872 2833 1606 Fragments arranged by size: 2833 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5311 AvaI C'yCGr_G Cuts at: 1870 1943 2322 2501 2636 1870 Size: 73 379 179 135 4545 Fragments arranged by size: 4545 379 179 135 73 AvrII C'CTAG_G Cuts at: 2536 2536 Size: 5311 BamHI G'GATC_C Cuts at: 2641 3008 2641 Size: 367 4944 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2082 2471 2632 4087 618 Size: 325 217 627 295 389 161 1455 1842 Fragments arranged by size: 1842 1455 627 389 325 295 217 161 BanII G_rGCy'C Cuts at: 1391 2193 2424 2453 2529 2630 1391 Size: 802 231 29 76 101 4072 Fragments arranged by size: 4072 802 231 101 76 29 BbeI G_GCGC'C Cuts at: 2086 2475 2086 Size: 389 4922 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4340 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3502 3536 1903 Size: 34 1565 34 3678 Fragments arranged by size: 3678 1565 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3203 4413 3203 Size: 1210 4101 BclI T'GATC_A Cuts at: 1548 1548 Size: 5311 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2175 2457 Size: 107 122 71 631 605 337 166 282 Cuts at: 2457 2469 3922 136 Size: 12 1453 1525 Fragments arranged by size: 1525 1453 631 605 337 282 166 122 107 71 12 BglII A'GATC_T Cuts at: 5306 5306 Size: 5311 BlpI GC'TnA_GC Cuts at: 2728 2728 Size: 5311 Bme1580I G_kGCm'C Cuts at: 1790 2453 2529 3372 4528 1790 Size: 663 76 843 1156 2573 Fragments arranged by size: 2573 1156 843 663 76 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5311 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3042 4000 442 Size: 416 53 83 399 24 1625 958 1753 Fragments arranged by size: 1753 1625 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5311 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2048 2393 2536 3971 1239 Size: 809 345 143 1435 2579 Fragments arranged by size: 2579 1435 809 345 143 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2177 3436 4301 4599 2177 Size: 1259 865 298 2889 Fragments arranged by size: 2889 1259 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3974 882 Size: 617 2475 2219 Fragments arranged by size: 2475 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3102 493 Size: 711 1898 2702 Fragments arranged by size: 2702 1898 711 BsaBI GATnn'nnATC Cuts at: 5305 5305 Size: 5311 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2083 2209 2472 3118 Size: 53 83 186 936 550 126 263 646 Cuts at: 3118 3500 4135 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 646 635 550 382 263 186 126 83 53 BsaWI w'CCGG_w Cuts at: 2575 3744 4142 4416 4563 4974 2575 Size: 1169 398 274 147 411 2912 Fragments arranged by size: 2912 1169 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5086 Fragments arranged by size: 5086 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 2060 2111 2402 1186 Size: 859 15 51 291 4095 Fragments arranged by size: 4095 859 291 51 15 BseYI C'CCAG_C Cuts at: 2434 2465 4514 5010 2434 Size: 31 2049 496 2735 Fragments arranged by size: 2735 2049 496 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2144 1163 Size: 573 408 4330 Fragments arranged by size: 4330 573 408 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2134 2761 3522 4550 1540 Size: 275 186 133 627 761 1028 2301 Fragments arranged by size: 2301 1028 761 627 275 186 133 BsiHKAI G_wGCw'C Cuts at: 2193 2424 3372 3457 4528 2193 Size: 231 948 85 1071 2976 Fragments arranged by size: 2976 1071 948 231 85 BsmI GAATG_Cn' Cuts at: 2822 2915 2822 Size: 93 5218 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2193 2424 2453 2529 2630 3372 3457 Size: 399 403 231 29 76 101 742 85 Cuts at: 3457 4528 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 403 399 231 101 85 76 29 BspEI T'CCGG_A Cuts at: 2575 4974 2575 Size: 2399 2912 BspHI T'CATG_A Cuts at: 3200 4930 3200 Size: 1730 3581 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2666 844 Size: 1822 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3198 1394 Size: 429 1375 3507 Fragments arranged by size: 3507 1375 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3806 3980 65 Size: 1183 2558 174 1396 Fragments arranged by size: 2558 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2205 3955 4142 1700 Size: 110 395 1750 187 2869 Fragments arranged by size: 2869 1750 395 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5311 BssHII G'CGCG_C Cuts at: 1925 2105 2281 1925 Size: 180 176 4955 Fragments arranged by size: 4955 180 176 BssSI C'ACGA_G Cuts at: 3371 4383 3371 Size: 1012 4299 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5311 BstBI TT'CG_AA Cuts at: 4115 4115 Size: 5311 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5311 BstYI r'GATC_y Cuts at: 1972 2181 2641 3008 3393 3410 4862 4948 4960 Size: 209 460 367 385 17 1452 86 12 Cuts at: 4960 5306 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 460 385 367 346 209 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2455 513 Size: 552 939 451 3369 Fragments arranged by size: 3369 939 552 451 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2556 487 Size: 683 56 1330 3242 Fragments arranged by size: 3242 1330 683 56 BtsI GCAGTG_nn' Cuts at: 2827 3621 3641 2827 Size: 794 20 4497 Fragments arranged by size: 4497 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5311 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5276 DraI TTT'AAA Cuts at: 2615 2615 Size: 5311 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4318 809 Size: 507 3002 1802 Fragments arranged by size: 3002 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2131 2364 2758 Size: 54 1425 50 275 18 301 233 394 Cuts at: 2758 3647 5276 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 394 301 275 233 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2131 2758 1537 Size: 275 319 627 4090 Fragments arranged by size: 4090 627 319 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2064 2309 3241 1353 Size: 711 245 932 3423 Fragments arranged by size: 3423 932 711 245 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3898 4272 4418 387 Size: 1064 138 2309 374 146 1280 Fragments arranged by size: 2309 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2191 2422 2191 Size: 231 5080 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2393 2536 3374 3971 4758 5026 Size: 393 416 345 143 838 597 787 268 Cuts at: 5026 1239 Size: 1524 Fragments arranged by size: 1524 838 787 597 416 393 345 268 143 EcoNI CCTnn'n_nnAGG Cuts at: 2141 2141 Size: 5311 EcoO109I rG'GnC_Cy Cuts at: 2310 2338 2449 2450 2526 2310 Size: 28 111 1 76 5095 Fragments arranged by size: 5095 111 76 28 1 EcoRI G'AATT_C Cuts at: 2620 2998 2620 Size: 378 4933 FspI TGC'GCA Cuts at: 3817 3817 Size: 5311 HaeII r_GCGC'y Cuts at: 1678 2060 2086 2269 2414 2435 2475 4458 1678 Size: 382 26 183 145 21 40 1983 2531 Fragments arranged by size: 2531 1983 382 183 145 40 26 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2534 2566 3952 3984 4026 Size: 32 135 32 962 32 1386 32 42 Cuts at: 4026 4058 1373 Size: 32 2626 Fragments arranged by size: 2626 1386 962 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2655 2901 669 Size: 988 281 717 246 3079 Fragments arranged by size: 3079 988 717 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5311 HpaI GTT'AAC Cuts at: 2901 2901 Size: 5311 KasI G'GCGC_C Cuts at: 2082 2471 2082 Size: 389 4922 KpnI G_GTAC'C Cuts at: 2636 2636 Size: 5311 MfeI C'AATT_G Cuts at: 2910 2910 Size: 5311 MluI A'CGCG_T Cuts at: 5187 5187 Size: 5311 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4425 4609 1305 Size: 3120 184 2007 Fragments arranged by size: 3120 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3489 Fragments arranged by size: 3489 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2293 3269 3628 3787 518 Size: 648 1127 976 359 159 2042 Fragments arranged by size: 2042 1127 976 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2161 2457 2469 2727 3404 4552 4797 1606 Size: 555 296 12 258 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 555 296 258 245 12 NarI GG'CG_CC Cuts at: 2083 2472 2083 Size: 389 4922 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3820 Fragments arranged by size: 3820 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5311 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5311 NotI GC'GGCC_GC Cuts at: 2131 2758 2131 Size: 627 4684 NspI r_CATG'y Cuts at: 1132 1229 2669 4214 1132 Size: 97 1440 1545 2229 Fragments arranged by size: 2229 1545 1440 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5311 PciI A'CATG_T Cuts at: 4210 4210 Size: 5311 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5311 PfoI T'CCnGG_A Cuts at: 2253 2253 Size: 5311 PmeI GTTT'AAAC Cuts at: 2615 2615 Size: 5311 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3380 3412 4893 4925 3380 Size: 32 1481 32 3766 Fragments arranged by size: 3766 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2310 2338 2310 Size: 28 5283 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5311 PsiI TTA'TAA Cuts at: 2881 2881 Size: 5311 PspOMI G'GGCC_C Cuts at: 2449 2525 2449 Size: 76 5235 PstI C_TGCA'G Cuts at: 830 2166 2217 2663 830 Size: 1336 51 446 3478 Fragments arranged by size: 3478 1336 446 51 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5311 PvuII CAG'CTG Cuts at: 2161 2161 Size: 5311 SacI G_AGCT'C Cuts at: 2193 2424 2193 Size: 231 5080 SacII CC_GC'GG Cuts at: 2458 2458 Size: 5311 SalI G'TCGA_C Cuts at: 1936 2653 1936 Size: 717 4594 SanDI GG'GwC_CC Cuts at: 2310 2310 Size: 5311 SapI GCTCTTCn'nnn_ Cuts at: 2064 2309 2064 Size: 245 5066 SbfI CC_TGCA'GG Cuts at: 2663 2663 Size: 5311 ScaI AGT'ACT Cuts at: 3559 5271 3559 Size: 1712 3599 SfcI C'TryA_G Cuts at: 826 1045 2162 2213 2659 3794 4475 4666 826 Size: 219 1117 51 446 1135 681 191 1471 Fragments arranged by size: 1471 1135 1117 681 446 219 191 51 SfoI GGC'GCC Cuts at: 2084 2473 2084 Size: 389 4922 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5311 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5311 SmaI CCC'GGG Cuts at: 1872 2324 2638 1872 Size: 452 314 4545 Fragments arranged by size: 4545 452 314 SmlI C'TyrA_G Cuts at: 820 1017 1943 2192 2414 3415 4316 4578 820 Size: 197 926 249 222 1001 901 262 1553 Fragments arranged by size: 1553 1001 926 901 262 249 222 197 SnaBI TAC'GTA Cuts at: 493 3102 493 Size: 2609 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5311 SphI G_CATG'C Cuts at: 1132 2669 1132 Size: 1537 3774 SspI AAT'ATT Cuts at: 5 52 3235 5 Size: 47 3183 2081 Fragments arranged by size: 3183 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5311 StyI C'CwwG_G Cuts at: 513 1065 2004 2536 2750 513 Size: 552 939 532 214 3074 Fragments arranged by size: 3074 939 552 532 214 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3338 3355 3508 3693 4138 325 Size: 1255 1758 17 153 185 445 1498 Fragments arranged by size: 1758 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3557 5269 96 Size: 274 80 33 51 1443 1580 1712 138 Fragments arranged by size: 1712 1580 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5271 5303 5271 Size: 32 5279 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5311 XbaI T'CTAG_A Cuts at: 2647 2647 Size: 5311 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5311 XmaI C'CCGG_G Cuts at: 1870 2322 2636 1870 Size: 452 314 4545 Fragments arranged by size: 4545 452 314 XmnI GAAnn'nnTTC Cuts at: 3440 3440 Size: 5311 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3119 276 Size: 53 83 186 936 1585 2468 Fragments arranged by size: 2468 1585 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SexAI SfiI SrfI SwaI XcmI