(Circular) (Six-base) MAPSORT of: pFN21AB9787.seq Check: 1049 from: 1 to: 5278 pFN21AB9787 5278 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2476 2476 Size: 5278 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2139 3088 278 Size: 53 83 186 936 603 949 2468 Fragments arranged by size: 2468 949 936 603 186 83 53 AccI GT'mk_AC Cuts at: 1937 2621 1937 Size: 684 4594 Acc65I G'GTAC_C Cuts at: 2599 2599 Size: 5278 AclI AA'CG_TT Cuts at: 1563 3405 3778 1563 Size: 1842 373 3063 Fragments arranged by size: 3063 1842 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2182 3341 4725 4993 1632 Size: 550 1159 1384 268 1917 Fragments arranged by size: 1917 1384 1159 550 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5081 AflIII A'CryG_T Cuts at: 1203 4177 5154 1203 Size: 2974 977 1327 Fragments arranged by size: 2974 1327 977 AgeI A'CCGG_T Cuts at: 4109 4109 Size: 5278 AhdI GACnn_n'nnGTC Cuts at: 4007 4007 Size: 5278 AleI CACnn'nnGTG Cuts at: 1166 2129 1166 Size: 963 4315 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5278 AlwNI CAG_nnn'CTG Cuts at: 3021 4593 3021 Size: 1572 3706 ApaI G_GGCC'C Cuts at: 2379 2379 Size: 5278 ApaLI G'TGCA_C Cuts at: 3335 4491 3335 Size: 1156 4122 ApoI r'AATT_y Cuts at: 1493 2587 2813 2965 5074 1493 Size: 1094 226 152 2109 1697 Fragments arranged by size: 2109 1697 1094 226 152 AseI AT'TA_AT Cuts at: 160 1032 3832 160 Size: 872 2800 1606 Fragments arranged by size: 2800 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5278 AvaI C'yCGr_G Cuts at: 1870 1943 2603 1870 Size: 73 660 4545 Fragments arranged by size: 4545 660 73 BamHI G'GATC_C Cuts at: 2608 2975 2608 Size: 367 4911 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2599 4054 618 Size: 325 217 627 812 1455 1842 Fragments arranged by size: 1842 1455 812 627 325 217 BanII G_rGCy'C Cuts at: 1391 2379 2597 1391 Size: 988 218 4072 Fragments arranged by size: 4072 988 218 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2131 928 Size: 971 232 4075 Fragments arranged by size: 4075 971 232 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2557 2591 3469 3503 1903 Size: 34 50 34 536 34 878 34 3678 Fragments arranged by size: 3678 878 536 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3170 4380 3170 Size: 1210 4068 BclI T'GATC_A Cuts at: 1548 1548 Size: 5278 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3889 136 Size: 107 122 71 631 605 2217 1525 Fragments arranged by size: 2217 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2571 5273 2571 Size: 2702 2576 BlpI GC'TnA_GC Cuts at: 2458 2695 2458 Size: 237 5041 Bme1580I G_kGCm'C Cuts at: 1790 2379 3339 4495 1790 Size: 589 960 1156 2573 Fragments arranged by size: 2573 1156 960 589 BmgBI CAC'GTC Cuts at: 1318 2236 1318 Size: 918 4360 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3009 3967 442 Size: 416 53 83 399 24 1592 958 1753 Fragments arranged by size: 1753 1592 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5278 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2471 3938 1239 Size: 1232 1467 2579 Fragments arranged by size: 2579 1467 1232 Bpu10I CC'TnA_GC Cuts at: 2465 2465 Size: 5278 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3403 4268 4566 3403 Size: 865 298 4115 Fragments arranged by size: 4115 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2078 3941 882 Size: 617 579 1863 2219 Fragments arranged by size: 2219 1863 617 579 BsaAI yAC'GTr Cuts at: 493 1204 3069 493 Size: 711 1865 2702 Fragments arranged by size: 2702 1865 711 BsaBI GATnn'nnATC Cuts at: 5272 5272 Size: 5278 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2136 2193 3085 3467 Size: 53 83 186 936 603 57 892 382 Cuts at: 3467 4102 275 Size: 635 1451 Fragments arranged by size: 1451 936 892 635 603 382 186 83 57 53 BsaWI w'CCGG_w Cuts at: 3711 4109 4383 4530 4941 3711 Size: 398 274 147 411 4048 Fragments arranged by size: 4048 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2372 2402 1178 Size: 30 165 30 969 30 4054 Fragments arranged by size: 4054 969 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5278 BseYI C'CCAG_C Cuts at: 4481 4977 4481 Size: 496 4782 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4705 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2728 3489 4517 1540 Size: 275 186 727 761 1028 2301 Fragments arranged by size: 2301 1028 761 727 275 186 BsiHKAI G_wGCw'C Cuts at: 2459 3339 3424 4495 2459 Size: 880 85 1071 3242 Fragments arranged by size: 3242 1071 880 85 BsmI GAATG_Cn' Cuts at: 2789 2882 2789 Size: 93 5185 BsmBI CGTCTCn'nnnn_ Cuts at: 2524 2524 Size: 5278 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2379 2459 2597 3339 3424 4495 1391 Size: 399 589 80 138 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 589 399 138 85 80 BspEI T'CCGG_A Cuts at: 4941 4941 Size: 5278 BspHI T'CATG_A Cuts at: 3167 4897 3167 Size: 1730 3548 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2476 2633 844 Size: 1632 157 3489 Fragments arranged by size: 3489 1632 157 BsrBI CCG'CTC Cuts at: 1394 1823 3165 1394 Size: 429 1342 3507 Fragments arranged by size: 3507 1342 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2194 3773 3947 65 Size: 1183 946 1579 174 1396 Fragments arranged by size: 1579 1396 1183 946 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2183 3922 4109 1700 Size: 110 373 1739 187 2869 Fragments arranged by size: 2869 1739 373 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5278 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5278 BssSI C'ACGA_G Cuts at: 3338 4350 3338 Size: 1012 4266 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5278 BstBI TT'CG_AA Cuts at: 4082 4082 Size: 5278 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5278 BstYI r'GATC_y Cuts at: 1972 2571 2608 2975 3360 3377 4829 4915 4927 Size: 599 37 367 385 17 1452 86 12 Cuts at: 4927 5273 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 599 385 367 346 86 37 17 12 BtgI C'CryG_G Cuts at: 513 1065 2403 513 Size: 552 1338 3388 Fragments arranged by size: 3388 1338 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4539 Fragments arranged by size: 4539 683 56 BtsI GCAGTG_nn' Cuts at: 2794 3588 3608 2794 Size: 794 20 4464 Fragments arranged by size: 4464 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5278 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5243 DraI TTT'AAA Cuts at: 2582 2582 Size: 5278 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4285 809 Size: 507 2969 1802 Fragments arranged by size: 2969 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2259 2346 2725 Size: 54 1425 50 275 18 429 87 379 Cuts at: 2725 3614 5243 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 429 379 275 87 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2725 1537 Size: 275 913 4090 Fragments arranged by size: 4090 913 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2555 3208 1353 Size: 1202 653 3423 Fragments arranged by size: 3423 1202 653 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3865 4239 4385 387 Size: 1064 138 2276 374 146 1280 Fragments arranged by size: 2276 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2182 2471 3341 3938 4725 4993 1239 Size: 393 550 289 870 597 787 268 1524 Fragments arranged by size: 1524 870 787 597 550 393 289 268 EcoO109I rG'GnC_Cy Cuts at: 2375 2376 2385 2375 Size: 1 9 5268 Fragments arranged by size: 5268 9 1 EcoRI G'AATT_C Cuts at: 2587 2965 2587 Size: 378 4900 FspI TGC'GCA Cuts at: 3784 3784 Size: 5278 HaeII r_GCGC'y Cuts at: 1678 4425 1678 Size: 2747 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3919 3951 3993 4025 1373 Size: 32 135 32 2347 32 42 32 2626 Fragments arranged by size: 2626 2347 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2518 2622 2868 669 Size: 988 281 580 104 246 3079 Fragments arranged by size: 3079 988 580 281 246 104 HindIII A'AGCT_T Cuts at: 748 748 Size: 5278 HpaI GTT'AAC Cuts at: 2868 2868 Size: 5278 KpnI G_GTAC'C Cuts at: 2603 2603 Size: 5278 MfeI C'AATT_G Cuts at: 2877 2877 Size: 5278 MluI A'CGCG_T Cuts at: 5154 5154 Size: 5278 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4392 4576 1305 Size: 3087 184 2007 Fragments arranged by size: 3087 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2261 2348 10 Size: 54 1425 343 429 87 2940 Fragments arranged by size: 2940 1425 429 343 87 54 MslI CAynn'nnrTG Cuts at: 518 1166 2129 3236 3595 3754 518 Size: 648 963 1107 359 159 2042 Fragments arranged by size: 2042 1107 963 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2365 2694 3371 4519 4764 1606 Size: 759 329 677 1148 245 2120 Fragments arranged by size: 2120 1148 759 677 329 245 NaeI GCC'GGC Cuts at: 2185 2185 Size: 5278 NcoI C'CATG_G Cuts at: 513 1065 2403 513 Size: 552 1338 3388 Fragments arranged by size: 3388 1338 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5278 NgoMIV G'CCGG_C Cuts at: 2183 2183 Size: 5278 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5278 NotI GC'GGCC_GC Cuts at: 2725 2725 Size: 5278 NspI r_CATG'y Cuts at: 1132 1229 2636 4181 1132 Size: 97 1407 1545 2229 Fragments arranged by size: 2229 1545 1407 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5278 PciI A'CATG_T Cuts at: 4177 4177 Size: 5278 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5278 PfoI T'CCnGG_A Cuts at: 2091 2091 Size: 5278 PmeI GTTT'AAAC Cuts at: 2582 2582 Size: 5278 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3347 3379 4860 4892 3347 Size: 32 1481 32 3733 Fragments arranged by size: 3733 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5278 PsiI TTA'TAA Cuts at: 2848 2848 Size: 5278 PspOMI G'GGCC_C Cuts at: 2375 2375 Size: 5278 PstI C_TGCA'G Cuts at: 830 2630 830 Size: 1800 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5278 SalI G'TCGA_C Cuts at: 1936 2620 1936 Size: 684 4594 SapI GCTCTTCn'nnn_ Cuts at: 2555 2555 Size: 5278 SbfI CC_TGCA'GG Cuts at: 2630 2630 Size: 5278 ScaI AGT'ACT Cuts at: 3526 5238 3526 Size: 1712 3566 SfcI C'TryA_G Cuts at: 826 1045 2626 3761 4442 4633 826 Size: 219 1581 1135 681 191 1471 Fragments arranged by size: 1581 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5278 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5278 SmaI CCC'GGG Cuts at: 1872 2605 1872 Size: 733 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3382 4283 4545 820 Size: 197 926 1439 901 262 1553 Fragments arranged by size: 1553 1439 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3069 493 Size: 2576 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5278 SphI G_CATG'C Cuts at: 1132 2636 1132 Size: 1504 3774 SspI AAT'ATT Cuts at: 5 52 3202 5 Size: 47 3150 2081 Fragments arranged by size: 3150 2081 47 StuI AGG'CCT Cuts at: 1517 2099 1517 Size: 582 4696 StyI C'CwwG_G Cuts at: 513 1065 2403 2439 2717 513 Size: 552 1338 36 278 3074 Fragments arranged by size: 3074 1338 552 278 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2366 3305 3322 3475 3660 4105 325 Size: 1255 786 939 17 153 185 445 1498 Fragments arranged by size: 1498 1255 939 786 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2479 3524 5236 Size: 274 80 33 51 1443 502 1045 1712 Cuts at: 5236 96 Size: 138 Fragments arranged by size: 1712 1443 1045 502 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2372 2404 5238 5270 2372 Size: 32 2834 32 2380 Fragments arranged by size: 2834 2380 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5278 XbaI T'CTAG_A Cuts at: 2614 2614 Size: 5278 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2446 2446 Size: 5278 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5278 XmaI C'CCGG_G Cuts at: 1870 2603 1870 Size: 733 4545 XmnI GAAnn'nnTTC Cuts at: 3407 3407 Size: 5278 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2137 3086 276 Size: 53 83 186 936 603 949 2468 Fragments arranged by size: 2468 949 936 603 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI