Restriction map for
pFN21AB9786
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccIII AcsI
AflII AflIII AgeI AhdI AluI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BpmI BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmAI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HgaI HindIII
HinfI HpaI KpnI Ksp632I KspI
MaeI MaeIII MamI MboII MfeI
MluI MroI MseI MslI MspA1I
MunI NaeI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tsp45I Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 123 CGCG CG^CG
AclI 226 AACGTT AA^CGTT
AlwI 444 GATCC GGATC(4/5)
AlwNI 427 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 526 GTGCTC G[AT]GC[AT]^C
BalI 434 TGGCCA TGG^CCA
BanII 359 GAGCCC G[GA]GC[CT]^C
BbvI 417 GCAGC GCAGC(8/12)
BcnI 519 CCGGG CC^[GC]GG
BlpI 470 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 470 GCTCAGC GC^T[ACGT]AGC
BsaJI 509 CCGAGG C^C[ACGT][ACGT]GG
BseRI 184 GAGGAG GAGGAG(10/8)
BsiHKAI 526 GTGCTC G[AT]GC[AT]^C
BsmFI 167 GGGAC GGGAC(10/14)
BsmI 466 GAATGC GAATGC(1/-1)
BsrDI 249 CATTGC GCAATG(2/0)
BssHII 122 GCGCGC G^CGCGC
BstUI 123 CGCG CG^CG
BstXI 326 CCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 443 AGATCC [GA]^GATC[CT]
CelII 470 GCTCAGC GC^T[ACGT]AGC
DraII 350 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 448 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 434 TGGCCA [CT]^GGCC[GA]
EcoNI 477 CCTGCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 350 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 175 GCGGG CCCGC(4/6)
HapII 519 CCGG C^CGG
HincII 136 GTTGAC GT[CT]^[GA]AC
HindII 136 GTTGAC GT[CT]^[GA]AC
HpaII 519 CCGG C^CGG
HphI 28 GGTGA GGTGA(8/7)
MflI 443 AGATCC [GA]^GATC[CT]
MluNI 434 TGGCCA TGG^CCA
MscI 434 TGGCCA TGG^CCA
MspI 519 CCGG C^CGG
MvnI 123 CGCG CG^CG
NciI 519 CCGGG CC^[GC]GG
PflMI 426 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 350 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 226 AACGTT AA^CGTT
SmlI 385 CTCAAG C^T[CT][GA]AG
TseI 417 GCAGC G^C[AT]GC
Tsp509I 115 AATT ^AATT
Van91I 426 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 326 CCATCCTGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 443 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 380 GGCGCC G[GA]^CG[CT]C
402 GGCGCC
AfaI 93 GTAC GT^AC
203 GTAC
AvaII 351 GGACC G^G[AT]CC
557 GGTCC
BbeI 380 GGCGCC GGCGC^C
402 GGCGCC
BsaHI 380 GGCGCC G[GA]^CG[CT]C
402 GGCGCC
BsrI 432 ACTGG ACTGG(1/-1)
254 CCAGT
BstAPI 8 GCATCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
44 GCAAGACGTGC
DpnII 444 GATC ^GATC
543 GATC
Fnu4HI 279 GCGGC GC^[ACGT]GC
417 GCAGC
HaeII 380 GGCGCC [GA]GCGC^[CT]
402 GGCGCC
Hin1I 380 GGCGCC G[GA]^CG[CT]C
402 GGCGCC
ItaI 279 GCGGC GC^[ACGT]GC
417 GCAGC
KasI 380 GGCGCC G^GCGCC
402 GGCGCC
MboI 444 GATC ^GATC
543 GATC
NarI 380 GGCGCC GG^CGCC
402 GGCGCC
NdeII 444 GATC ^GATC
543 GATC
RsaI 93 GTAC GT^AC
203 GTAC
Sau3AI 444 GATC ^GATC
543 GATC
SfoI 380 GGCGCC GGC^GCC
402 GGCGCC
TaqI 110 TCGA T^CGA
464 TCGA
TthHB8I 110 TCGA T^CGA
464 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI BanI BmyI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI EcoRII FokI
HaeIII HhaI HinP1I MaeII MnlI
MvaI MwoI NlaIV Sau96I ScrFI
SfaNI TspRI