Restriction map for
pFN21AB9781
##################################
# NO CUT ENZYMES ( 194 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BbvI BcgI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsoBI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DpnII
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoT22I FauI
FbaI Fnu4HI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaI HphI
ItaI KasI KpnI KspI MaeI
MaeIII MamI MboI MfeI MflI
MluI MluNI MroI MscI MseI
MspA1I MunI MvnI NarI NcoI
NdeI NdeII NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI Sau3AI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TfiI Tru9I TseI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AfaI 213 GTAC GT^AC
AvaII 237 GGACC G^G[AT]CC
BanII 122 GAGCCC G[GA]GC[CT]^C
BbsI 333 GAAGAC GAAGAC(2/6)
BciVI 163 GTATCC GTATCC(6/5)
BcnI 40 CCCGG CC^[GC]GG
BpmI 278 CTGGAG CTGGAG(16/14)
BpuAI 333 GAAGAC GAAGAC(2/6)
BseRI 93 GAGGAG GAGGAG(10/8)
BsmI 115 GAATGC GAATGC(1/-1)
Bsp1407I 212 TGTACA T^GTACA
BsrDI 232 GCAATG GCAATG(2/0)
BsrFI 98 GCCGGC [GA]^CCGG[CT]
BsrGI 212 TGTACA T^GTACA
BstF5I 3 GGATG GGATG(2/0)
BstNI 356 CCTGG CC^[AT]GG
Cfr10I 98 GCCGGC [GA]^CCGG[CT]
DraII 266 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoNI 192 CCTCAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 266 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRII 356 CCTGG ^CC[AT]GG
EcoRV 140 GATATC GAT^ATC
FokI 3 GGATG GGATG(9/13)
HaeIII 267 GGCC GG^CC
MaeII 48 ACGT A^CGT
MslI 228 CATGGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 356 CCTGG CC^[AT]GG
MwoI 181 GCTATATGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 98 GCCGGC GCC^GGC
NciI 40 CCCGG CC^[GC]GG
NgoMIV 98 GCCGGC G^CCGGC
NlaIII 228 CATG CATG^
NlaIV 95 GGAGCC GG[ACGT]^[ACGT]CC
RsaI 213 GTAC GT^AC
SapI 283 GAAGAGC GCTCTTC(1/4)
SfaNI 340 GATGC GCATC(5/9)
SspBI 212 TGTACA T^GTACA
TaqI 171 TCGA T^CGA
TthHB8I 171 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AluI 134 AGCT AG^CT
180 AGCT
BmyI 122 GAGCCC G[AGT]GC[ACT]^C
188 GTGCCC
BsaJI 240 CCAAGG C^C[ACGT][ACGT]GG
323 CCAAGG
BsiYI 192 CCTCAGAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
323 CCAAGGCTGGG
BslI 192 CCTCAGAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
323 CCAAGGCTGGG
Bsp1286I 122 GAGCCC G[AGT]GC[ACT]^C
188 GTGCCC
BssKI 40 CCCGG ^CC[ACGT]GG
356 CCTGG
Cfr13I 237 GGACC G^G[ACGT]CC
267 GGCCC
EarI 154 GAAGAG CTCTTC(1/4)
283 GAAGAG
EcoT14I 240 CCAAGG C^C[AT][AT]GG
323 CCAAGG
HapII 41 CCGG C^CGG
99 CCGG
HpaII 41 CCGG C^CGG
99 CCGG
Ksp632I 154 GAAGAG CTCTTC(1/4)
283 GAAGAG
MspI 41 CCGG C^CGG
99 CCGG
Sau96I 237 GGACC G^G[ACGT]CC
267 GGCCC
ScrFI 40 CCCGG CC^[ACGT]GG
356 CCTGG
SmlI 88 CTTGAG C^T[CT][GA]AG
309 CTCAAG
StyI 240 CCAAGG C^C[AT][AT]GG
323 CCAAGG
Tsp509I 69 AATT ^AATT
352 AATT
TspRI 220 CAGTG CA[GC]TG[ACGT][ACGT]^
276 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 5 enzymes) #
#########################################
BsrI Cac8I DdeI MboII MnlI