(Circular) (Six-base) MAPSORT of: pFN21AB9779.seq Check: 9047 from: 1 to: 7366 pFN21AB9779 7366 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4437 4437 Size: 7366 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5176 278 Size: 53 83 186 936 3640 2468 Fragments arranged by size: 3640 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4709 1937 Size: 2772 4594 Acc65I G'GTAC_C Cuts at: 4687 4687 Size: 7366 AclI AA'CG_TT Cuts at: 1563 5493 5866 1563 Size: 3930 373 3063 Fragments arranged by size: 3930 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3342 4098 5429 6813 7081 1632 Size: 1710 756 1331 1384 268 1917 Fragments arranged by size: 1917 1710 1384 1331 756 268 AflII C'TTAA_G Cuts at: 820 1017 2066 820 Size: 197 1049 6120 Fragments arranged by size: 6120 1049 197 AflIII A'CryG_T Cuts at: 1203 6265 7242 1203 Size: 5062 977 1327 Fragments arranged by size: 5062 1327 977 AgeI A'CCGG_T Cuts at: 6197 6197 Size: 7366 AhdI GACnn_n'nnGTC Cuts at: 6095 6095 Size: 7366 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7366 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7366 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4448 4480 4448 Size: 32 7334 AlwNI CAG_nnn'CTG Cuts at: 2366 4070 4588 5109 6681 2366 Size: 1704 518 521 1572 3051 Fragments arranged by size: 3051 1704 1572 521 518 ApaLI G'TGCA_C Cuts at: 5423 6579 5423 Size: 1156 6210 ApoI r'AATT_y Cuts at: 1493 2663 2794 2999 3223 3298 3310 3754 4538 Size: 1170 131 205 224 75 12 444 784 Cuts at: 4538 4675 4901 5053 7162 1493 Size: 137 226 152 2109 1697 Fragments arranged by size: 2109 1697 1170 784 444 226 224 205 152 137 131 75 12 AseI AT'TA_AT Cuts at: 160 1032 5920 160 Size: 872 4888 1606 Fragments arranged by size: 4888 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7366 AvaI C'yCGr_G Cuts at: 1870 1943 4691 1870 Size: 73 2748 4545 Fragments arranged by size: 4545 2748 73 BamHI G'GATC_C Cuts at: 4309 4696 5063 4309 Size: 387 367 6612 Fragments arranged by size: 6612 387 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4236 4687 6142 618 Size: 325 217 627 2449 451 1455 1842 Fragments arranged by size: 2449 1842 1455 627 451 325 217 BanII G_rGCy'C Cuts at: 1391 4685 1391 Size: 3294 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2130 928 Size: 971 231 6164 Fragments arranged by size: 6164 971 231 BbvCI CC'TCA_GC Cuts at: 4161 4161 Size: 7366 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5557 5591 1903 Size: 34 3620 34 3678 Fragments arranged by size: 3678 3620 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5258 6468 5258 Size: 1210 6156 BclI T'GATC_A Cuts at: 1548 4206 1548 Size: 2658 4708 BfrBI ATG'CAT Cuts at: 2425 2425 Size: 7366 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2321 3044 5977 Size: 107 122 71 631 605 649 723 2933 Cuts at: 5977 136 Size: 1525 Fragments arranged by size: 2933 1525 723 649 631 605 122 107 71 BglII A'GATC_T Cuts at: 2944 3424 7361 2944 Size: 480 3937 2949 Fragments arranged by size: 3937 2949 480 BlpI GC'TnA_GC Cuts at: 4783 4783 Size: 7366 Bme1580I G_kGCm'C Cuts at: 1790 3850 4373 5427 6583 1790 Size: 2060 523 1054 1156 2573 Fragments arranged by size: 2573 2060 1156 1054 523 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7366 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4645 5097 6055 Size: 416 53 83 399 24 3228 452 958 Cuts at: 6055 442 Size: 1753 Fragments arranged by size: 3228 1753 958 452 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7366 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2182 2182 Size: 7366 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4678 6026 1239 Size: 3439 1348 2579 Fragments arranged by size: 3439 2579 1348 Bpu10I CC'TnA_GC Cuts at: 4161 4161 Size: 7366 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3015 5491 6356 6654 3015 Size: 2476 865 298 3727 Fragments arranged by size: 3727 2476 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6029 882 Size: 617 4530 2219 Fragments arranged by size: 4530 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5157 493 Size: 711 3953 2702 Fragments arranged by size: 3953 2702 711 BsaBI GATnn'nnATC Cuts at: 7360 7360 Size: 7366 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5173 5555 6190 275 Size: 53 83 186 936 3640 382 635 1451 Fragments arranged by size: 3640 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3820 5799 6197 6471 6618 7029 3820 Size: 1979 398 274 147 411 4157 Fragments arranged by size: 4157 1979 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2464 2494 1178 Size: 30 165 30 1061 30 6050 Fragments arranged by size: 6050 1061 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3363 1186 Size: 2177 5189 BseYI C'CCAG_C Cuts at: 6569 7065 6569 Size: 496 6870 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3618 3843 1163 Size: 573 1882 225 4686 Fragments arranged by size: 4686 1882 573 225 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4816 5577 6605 1540 Size: 275 186 2815 761 1028 2301 Fragments arranged by size: 2815 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5427 5512 6583 5427 Size: 85 1071 6210 Fragments arranged by size: 6210 1071 85 BsmI GAATG_Cn' Cuts at: 2317 4101 4877 4970 2317 Size: 1784 776 93 4713 Fragments arranged by size: 4713 1784 776 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3850 4373 4685 5427 5512 6583 1391 Size: 399 2060 523 312 742 85 1071 2174 Fragments arranged by size: 2174 2060 1071 742 523 399 312 85 BspEI T'CCGG_A Cuts at: 3820 7029 3820 Size: 3209 4157 BspHI T'CATG_A Cuts at: 5255 6985 5255 Size: 1730 5636 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2310 4437 4721 844 Size: 1466 2127 284 3489 Fragments arranged by size: 3489 2127 1466 284 BsrBI CCG'CTC Cuts at: 1394 1823 5253 1394 Size: 429 3430 3507 Fragments arranged by size: 3507 3430 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3728 5861 6035 65 Size: 1183 2480 2133 174 1396 Fragments arranged by size: 2480 2133 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6010 6197 1700 Size: 110 4200 187 2869 Fragments arranged by size: 4200 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7366 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7366 BssSI C'ACGA_G Cuts at: 2878 5426 6438 2878 Size: 2548 1012 3806 Fragments arranged by size: 3806 2548 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4579 794 Size: 3785 3581 BstBI TT'CG_AA Cuts at: 6170 6170 Size: 7366 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7366 BstYI r'GATC_y Cuts at: 1972 2944 3022 3424 3532 4309 4696 5063 5448 Size: 972 78 402 108 777 387 367 385 Cuts at: 5448 5465 6917 7003 7015 7361 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 972 777 402 387 385 367 346 108 86 78 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5875 Fragments arranged by size: 5875 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6627 Fragments arranged by size: 6627 683 56 BtsI GCAGTG_nn' Cuts at: 2367 4629 4882 5676 5696 2367 Size: 2262 253 794 20 4037 Fragments arranged by size: 4037 2262 794 253 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7366 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7331 DraI TTT'AAA Cuts at: 3841 4670 3841 Size: 829 6537 DraIII CAC_nnn'GTG Cuts at: 2566 4133 4379 2566 Size: 1567 246 5553 Fragments arranged by size: 5553 1567 246 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2435 6373 809 Size: 507 1119 3938 1802 Fragments arranged by size: 3938 1802 1119 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4813 5702 7331 Size: 54 1425 50 275 18 2983 889 1629 Cuts at: 7331 8 Size: 43 Fragments arranged by size: 2983 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4813 1537 Size: 275 3001 4090 Fragments arranged by size: 4090 3001 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2084 3370 4280 4326 4557 5296 1353 Size: 731 1286 910 46 231 739 3423 Fragments arranged by size: 3423 1286 910 739 731 231 46 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5953 6327 6473 387 Size: 1064 138 4364 374 146 1280 Fragments arranged by size: 4364 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3342 4098 4678 5429 6026 6813 7081 Size: 393 1710 756 580 751 597 787 268 Cuts at: 7081 1239 Size: 1524 Fragments arranged by size: 1710 1524 787 756 751 597 580 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2370 2370 Size: 7366 EcoRI G'AATT_C Cuts at: 4675 5053 4675 Size: 378 6988 EcoRV GAT'ATC Cuts at: 2053 3444 2053 Size: 1391 5975 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2862 3956 2862 Size: 1094 6272 FspI TGC'GCA Cuts at: 5872 5872 Size: 7366 HaeII r_GCGC'y Cuts at: 1678 6513 1678 Size: 4835 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2509 2541 2717 2749 3089 Size: 32 135 32 937 32 176 32 340 Cuts at: 3089 3121 6007 6039 6081 6113 1373 Size: 32 2886 32 42 32 2626 Fragments arranged by size: 2886 2626 937 340 176 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4710 4956 669 Size: 988 281 2772 246 3079 Fragments arranged by size: 3079 2772 988 281 246 HindIII A'AGCT_T Cuts at: 748 2077 2819 748 Size: 1329 742 5295 Fragments arranged by size: 5295 1329 742 HpaI GTT'AAC Cuts at: 4956 4956 Size: 7366 KpnI G_GTAC'C Cuts at: 4691 4691 Size: 7366 MfeI C'AATT_G Cuts at: 3197 4965 3197 Size: 1768 5598 MluI A'CGCG_T Cuts at: 7242 7242 Size: 7366 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4207 4536 6480 6664 1305 Size: 2902 329 1944 184 2007 Fragments arranged by size: 2902 2007 1944 329 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5544 Fragments arranged by size: 5544 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3918 5324 5683 5842 518 Size: 648 2752 1406 359 159 2042 Fragments arranged by size: 2752 2042 1406 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4782 5459 6607 6852 1606 Size: 3176 677 1148 245 2120 Fragments arranged by size: 3176 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5875 Fragments arranged by size: 5875 939 552 NdeI CA'TA_TG Cuts at: 387 2333 2932 387 Size: 1946 599 4821 Fragments arranged by size: 4821 1946 599 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7366 NotI GC'GGCC_GC Cuts at: 4813 4813 Size: 7366 NsiI A_TGCA'T Cuts at: 2427 2427 Size: 7366 NspI r_CATG'y Cuts at: 1132 1229 2166 2410 4582 4724 6269 1132 Size: 97 937 244 2172 142 1545 2229 Fragments arranged by size: 2229 2172 1545 937 244 142 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7366 PciI A'CATG_T Cuts at: 6265 6265 Size: 7366 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7366 PfoI T'CCnGG_A Cuts at: 2118 2350 2118 Size: 232 7134 PmeI GTTT'AAAC Cuts at: 4670 4670 Size: 7366 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3411 3443 5435 5467 6948 6980 3411 Size: 32 1992 32 1481 32 3797 Fragments arranged by size: 3797 1992 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7366 PsiI TTA'TAA Cuts at: 2109 3341 4014 4936 2109 Size: 1232 673 922 4539 Fragments arranged by size: 4539 1232 922 673 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4379 4411 4379 Size: 32 7334 PstI C_TGCA'G Cuts at: 830 3319 4624 4718 830 Size: 2489 1305 94 3478 Fragments arranged by size: 3478 2489 1305 94 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7366 SalI G'TCGA_C Cuts at: 1936 4708 1936 Size: 2772 4594 SbfI CC_TGCA'GG Cuts at: 4718 4718 Size: 7366 ScaI AGT'ACT Cuts at: 4655 5614 7326 4655 Size: 959 1712 4695 Fragments arranged by size: 4695 1712 959 SfcI C'TryA_G Cuts at: 826 1045 2035 2367 3315 3722 4620 4714 5849 Size: 219 990 332 948 407 898 94 1135 Cuts at: 5849 6530 6721 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 990 948 898 681 407 332 219 191 94 SfiI GGCCn_nnn'nGGCC Cuts at: 3044 3044 Size: 7366 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7366 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7366 SmaI CCC'GGG Cuts at: 1872 4693 1872 Size: 2821 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2066 3030 5470 6371 6633 820 Size: 197 926 123 964 2440 901 262 1553 Fragments arranged by size: 2440 1553 964 926 901 262 197 123 SnaBI TAC'GTA Cuts at: 493 5157 493 Size: 4664 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7366 SphI G_CATG'C Cuts at: 1132 4724 1132 Size: 3592 3774 SspI AAT'ATT Cuts at: 5 52 2896 5290 5 Size: 47 2844 2394 2081 Fragments arranged by size: 2844 2394 2081 47 StuI AGG'CCT Cuts at: 1517 3047 1517 Size: 1530 5836 StyI C'CwwG_G Cuts at: 513 1065 2004 2416 3048 3093 4805 513 Size: 552 939 412 632 45 1712 3074 Fragments arranged by size: 3074 1712 939 632 552 412 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5393 5410 5563 5748 6193 325 Size: 1255 3813 17 153 185 445 1498 Fragments arranged by size: 3813 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2249 3552 3679 Size: 274 80 33 51 1443 272 1303 127 Cuts at: 3679 4031 4653 5612 7324 96 Size: 352 622 959 1712 138 Fragments arranged by size: 1712 1443 1303 959 622 352 274 272 138 127 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2346 2378 2464 2496 2550 2582 4188 4220 7326 Size: 32 86 32 54 32 1606 32 3106 Cuts at: 7326 7358 2346 Size: 32 2354 Fragments arranged by size: 3106 2354 1606 86 54 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7366 XbaI T'CTAG_A Cuts at: 4702 4702 Size: 7366 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3046 4286 3046 Size: 1240 6126 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7366 XmaI C'CCGG_G Cuts at: 1870 4691 1870 Size: 2821 4545 XmnI GAAnn'nnTTC Cuts at: 3310 4438 4635 5495 3310 Size: 1128 197 860 5181 Fragments arranged by size: 5181 1128 860 197 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5174 276 Size: 53 83 186 936 3640 2468 Fragments arranged by size: 3640 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI