(Circular) (Six-base) MAPSORT of: pFN21AB9775.seq Check: 1293 from: 1 to: 6739 pFN21AB9775 6739 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3993 3993 Size: 6739 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2301 4549 278 Size: 53 83 186 936 765 2248 2468 Fragments arranged by size: 2468 2248 936 765 186 83 53 AccI GT'mk_AC Cuts at: 1937 2280 4082 1937 Size: 343 1802 4594 Fragments arranged by size: 4594 1802 343 Acc65I G'GTAC_C Cuts at: 4060 4060 Size: 6739 AclI AA'CG_TT Cuts at: 1563 4866 5239 1563 Size: 3303 373 3063 Fragments arranged by size: 3303 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2228 2280 2448 2516 3140 3742 4802 6186 Size: 596 52 168 68 624 602 1060 1384 Cuts at: 6186 6454 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1060 624 602 596 268 168 68 52 AfeI AGC'GCT Cuts at: 2045 3536 2045 Size: 1491 5248 AflII C'TTAA_G Cuts at: 820 1017 2794 820 Size: 197 1777 4765 Fragments arranged by size: 4765 1777 197 AflIII A'CryG_T Cuts at: 1203 5638 6615 1203 Size: 4435 977 1327 Fragments arranged by size: 4435 1327 977 AgeI A'CCGG_T Cuts at: 5570 5570 Size: 6739 AhdI GACnn_n'nnGTC Cuts at: 5468 5468 Size: 6739 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6739 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6739 AlwNI CAG_nnn'CTG Cuts at: 2062 2359 2494 3028 3311 3349 3601 4482 6054 Size: 297 135 534 283 38 252 881 1572 Cuts at: 6054 2062 Size: 2747 Fragments arranged by size: 2747 1572 881 534 297 283 252 135 38 ApaLI G'TGCA_C Cuts at: 4796 5952 4796 Size: 1156 5583 ApoI r'AATT_y Cuts at: 1493 2420 2992 4048 4274 4426 6535 1493 Size: 927 572 1056 226 152 2109 1697 Fragments arranged by size: 2109 1697 1056 927 572 226 152 AseI AT'TA_AT Cuts at: 160 1032 5293 160 Size: 872 4261 1606 Fragments arranged by size: 4261 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6739 AvaI C'yCGr_G Cuts at: 1870 1943 4064 1870 Size: 73 2121 4545 Fragments arranged by size: 4545 2121 73 BamHI G'GATC_C Cuts at: 4069 4436 4069 Size: 367 6372 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2854 3389 3476 4060 5515 Size: 325 217 627 1067 535 87 584 1455 Cuts at: 5515 618 Size: 1842 Fragments arranged by size: 1842 1455 1067 627 584 535 325 217 87 BanII G_rGCy'C Cuts at: 1391 3426 4058 1391 Size: 2035 632 4072 Fragments arranged by size: 4072 2035 632 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3892 928 Size: 971 1993 3775 Fragments arranged by size: 3775 1993 971 BbvCI CC'TCA_GC Cuts at: 3818 3818 Size: 6739 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4930 4964 1903 Size: 34 2993 34 3678 Fragments arranged by size: 3678 2993 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4631 5841 4631 Size: 1210 5529 BclI T'GATC_A Cuts at: 1548 2730 1548 Size: 1182 5557 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5350 136 Size: 107 122 71 631 605 337 3341 1525 Fragments arranged by size: 3341 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3006 6734 3006 Size: 3728 3011 BlpI GC'TnA_GC Cuts at: 2616 3029 3396 4156 2616 Size: 413 367 760 5199 Fragments arranged by size: 5199 760 413 367 Bme1580I G_kGCm'C Cuts at: 1790 3481 3547 4800 5956 1790 Size: 1691 66 1253 1156 2573 Fragments arranged by size: 2573 1691 1253 1156 66 BmgBI CAC'GTC Cuts at: 1318 2863 2953 1318 Size: 1545 90 5104 Fragments arranged by size: 5104 1545 90 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3718 4470 5428 Size: 416 53 83 399 24 2301 752 958 Cuts at: 5428 442 Size: 1753 Fragments arranged by size: 2301 1753 958 752 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6739 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2157 2324 2627 2813 2878 2948 3200 3215 Size: 918 167 303 186 65 70 252 15 Cuts at: 3215 3359 3428 3505 3578 5399 1239 Size: 144 69 77 73 1821 2579 Fragments arranged by size: 2579 1821 918 303 252 186 167 144 77 73 70 69 65 15 Bpu10I CC'TnA_GC Cuts at: 3254 3350 3788 3818 3254 Size: 96 438 30 6175 Fragments arranged by size: 6175 438 96 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2543 3038 3581 3814 4864 5729 6027 2543 Size: 495 543 233 1050 865 298 3255 Fragments arranged by size: 3255 1050 865 543 495 298 233 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2783 5402 882 Size: 617 1284 2619 2219 Fragments arranged by size: 2619 2219 1284 617 BsaAI yAC'GTr Cuts at: 493 1204 4530 493 Size: 711 3326 2702 Fragments arranged by size: 3326 2702 711 BsaBI GATnn'nnATC Cuts at: 6733 6733 Size: 6739 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2298 3829 4546 4928 Size: 53 83 186 936 765 1531 717 382 Cuts at: 4928 5563 275 Size: 635 1451 Fragments arranged by size: 1531 1451 936 765 717 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5172 5570 5844 5991 6402 5172 Size: 398 274 147 411 5509 Fragments arranged by size: 5509 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2322 2352 3173 3203 3698 Size: 30 165 30 919 30 821 30 495 Cuts at: 3698 3728 3734 3764 1178 Size: 30 6 30 4153 Fragments arranged by size: 4153 919 821 495 165 30 30 30 30 30 30 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3742 3746 1186 Size: 2556 4 4179 Fragments arranged by size: 4179 2556 4 BseYI C'CCAG_C Cuts at: 3152 3582 5942 6438 3152 Size: 430 2360 496 3453 Fragments arranged by size: 3453 2360 496 430 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2345 2372 2975 3288 3872 3971 1163 Size: 573 609 27 603 313 584 99 3931 Fragments arranged by size: 3931 609 603 584 573 313 99 27 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4189 4950 5978 1540 Size: 275 186 2188 761 1028 2301 Fragments arranged by size: 2301 2188 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3402 3912 4800 4885 5956 3402 Size: 510 888 85 1071 4185 Fragments arranged by size: 4185 1071 888 510 85 BsmI GAATG_Cn' Cuts at: 4250 4343 4250 Size: 93 6646 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3402 3426 3481 3547 3912 4058 4800 Size: 399 1612 24 55 66 365 146 742 Cuts at: 4800 4885 5956 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1612 1071 742 399 365 146 85 66 55 24 BspEI T'CCGG_A Cuts at: 6402 6402 Size: 6739 BspHI T'CATG_A Cuts at: 2733 4628 6358 2733 Size: 1895 1730 3114 Fragments arranged by size: 3114 1895 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2182 3466 3993 4027 4094 844 Size: 1338 1284 527 34 67 3489 Fragments arranged by size: 3489 1338 1284 527 67 34 BsrBI CCG'CTC Cuts at: 1394 1823 2892 2926 3207 3258 3772 4626 1394 Size: 429 1069 34 281 51 514 854 3507 Fragments arranged by size: 3507 1069 854 514 429 281 51 34 BsrDI GCAATG_nn' Cuts at: 65 1248 5234 5408 65 Size: 1183 3986 174 1396 Fragments arranged by size: 3986 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2103 5383 5570 1700 Size: 110 293 3280 187 2869 Fragments arranged by size: 3280 2869 293 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6739 BssHII G'CGCG_C Cuts at: 1925 3625 3854 1925 Size: 1700 229 4810 Fragments arranged by size: 4810 1700 229 BssSI C'ACGA_G Cuts at: 3998 4799 5811 3998 Size: 801 1012 4926 Fragments arranged by size: 4926 1012 801 BstAPI GCAn_nnn'nTGC Cuts at: 794 2359 794 Size: 1565 5174 BstBI TT'CG_AA Cuts at: 5543 5543 Size: 6739 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6739 BstXI CCAn_nnnn'nTGG Cuts at: 3178 3850 3178 Size: 672 6067 BstYI r'GATC_y Cuts at: 1972 2305 2323 2844 3006 3414 4069 4436 4821 Size: 333 18 521 162 408 655 367 385 Cuts at: 4821 4838 6290 6376 6388 6734 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 655 521 408 385 367 346 333 162 86 18 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3126 513 Size: 552 939 1122 4126 Fragments arranged by size: 4126 1122 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6000 Fragments arranged by size: 6000 683 56 BtsI GCAGTG_nn' Cuts at: 3438 3503 3700 3889 4255 5049 5069 3438 Size: 65 197 189 366 794 20 5108 Fragments arranged by size: 5108 794 366 197 189 65 20 ClaI AT'CG_AT Cuts at: 1554 2024 1554 Size: 470 6269 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6704 DraI TTT'AAA Cuts at: 4043 4043 Size: 6739 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2587 5746 809 Size: 507 1271 3159 1802 Fragments arranged by size: 3159 1802 1271 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2814 2913 Size: 54 1425 50 275 18 180 804 99 Cuts at: 2913 2997 3168 3300 3679 4186 5075 6704 8 Size: 84 171 132 379 507 889 1629 43 Fragments arranged by size: 1629 1425 889 804 507 379 275 180 171 132 99 84 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4186 1537 Size: 275 2374 4090 Fragments arranged by size: 4090 2374 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2204 3087 4669 1353 Size: 851 883 1582 3423 Fragments arranged by size: 3423 1582 883 851 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2801 5326 5700 5846 387 Size: 1064 138 1212 2525 374 146 1280 Fragments arranged by size: 2525 1280 1212 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2157 2228 2280 2324 2448 2516 2627 Size: 393 525 71 52 44 124 68 111 Cuts at: 2627 2813 2878 2948 3140 3200 3215 3359 3428 Size: 186 65 70 192 60 15 144 69 Cuts at: 3428 3505 3578 3742 4802 5399 6186 6454 1239 Size: 77 73 164 1060 597 787 268 1524 Fragments arranged by size: 1524 1060 787 597 525 393 268 192 186 164 144 124 111 77 73 71 70 69 68 65 60 52 44 15 EcoNI CCTnn'n_nnAGG Cuts at: 3341 3485 3341 Size: 144 6595 EcoO109I rG'GnC_Cy Cuts at: 3346 3598 3346 Size: 252 6487 EcoRI G'AATT_C Cuts at: 2992 4048 4426 2992 Size: 1056 378 5305 Fragments arranged by size: 5305 1056 378 FspI TGC'GCA Cuts at: 2186 3236 5245 2186 Size: 1050 2009 3680 Fragments arranged by size: 3680 2009 1050 HaeII r_GCGC'y Cuts at: 1678 2047 2201 2359 3036 3153 3538 3739 5886 Size: 369 154 158 677 117 385 201 2147 Cuts at: 5886 1678 Size: 2531 Fragments arranged by size: 2531 2147 677 385 369 201 158 154 117 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2322 2354 3312 3344 3734 Size: 32 135 32 750 32 958 32 390 Cuts at: 3734 3766 3781 3813 5380 5412 5454 5486 1373 Size: 32 15 32 1567 32 42 32 2626 Fragments arranged by size: 2626 1567 958 750 390 135 42 32 32 32 32 32 32 32 32 15 HincII GTy'rAC Cuts at: 669 1657 1938 4083 4329 669 Size: 988 281 2145 246 3079 Fragments arranged by size: 3079 2145 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6739 HpaI GTT'AAC Cuts at: 4329 4329 Size: 6739 KpnI G_GTAC'C Cuts at: 4064 4064 Size: 6739 MfeI C'AATT_G Cuts at: 4338 4338 Size: 6739 MluI A'CGCG_T Cuts at: 6615 6615 Size: 6739 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3267 3867 5853 6037 1305 Size: 1962 600 1986 184 2007 Fragments arranged by size: 2007 1986 1962 600 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3302 10 Size: 54 1425 343 1470 3447 Fragments arranged by size: 3447 1470 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2879 4002 4697 5056 5215 518 Size: 648 1713 1123 695 359 159 2042 Fragments arranged by size: 2042 1713 1123 695 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2062 2203 2833 2941 3028 3373 3395 3474 Size: 456 141 630 108 87 345 22 79 Cuts at: 3474 3690 4155 4832 5980 6225 1606 Size: 216 465 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 630 465 456 345 245 216 141 108 87 79 22 NcoI C'CATG_G Cuts at: 513 1065 2004 3126 513 Size: 552 939 1122 4126 Fragments arranged by size: 4126 1122 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6739 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6739 NotI GC'GGCC_GC Cuts at: 4186 4186 Size: 6739 NspI r_CATG'y Cuts at: 1132 1229 4097 5642 1132 Size: 97 2868 1545 2229 Fragments arranged by size: 2868 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3801 1348 Size: 2453 4286 PciI A'CATG_T Cuts at: 5638 5638 Size: 6739 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6739 PmeI GTTT'AAAC Cuts at: 4043 4043 Size: 6739 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4808 4840 6321 6353 4808 Size: 32 1481 32 5194 Fragments arranged by size: 5194 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3346 3346 Size: 6739 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6739 PsiI TTA'TAA Cuts at: 4309 4309 Size: 6739 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2523 2555 2523 Size: 32 6707 PstI C_TGCA'G Cuts at: 830 2067 2577 3216 3463 3606 3669 4091 830 Size: 1237 510 639 247 143 63 422 3478 Fragments arranged by size: 3478 1237 639 510 422 247 143 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6739 PvuII CAG'CTG Cuts at: 2062 2833 2941 3028 2062 Size: 771 108 87 5773 Fragments arranged by size: 5773 771 108 87 SalI G'TCGA_C Cuts at: 1936 4081 1936 Size: 2145 4594 SbfI CC_TGCA'GG Cuts at: 4091 4091 Size: 6739 ScaI AGT'ACT Cuts at: 4987 6699 4987 Size: 1712 5027 SfcI C'TryA_G Cuts at: 826 1045 2063 2573 3212 3459 3602 3665 4087 Size: 219 1018 510 639 247 143 63 422 Cuts at: 4087 5222 5903 6094 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1018 681 639 510 422 247 219 191 143 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6739 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6739 SmaI CCC'GGG Cuts at: 1872 4066 1872 Size: 2194 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2522 2794 3017 3560 3793 4843 Size: 197 926 579 272 223 543 233 1050 Cuts at: 4843 5744 6006 820 Size: 901 262 1553 Fragments arranged by size: 1553 1050 926 901 579 543 272 262 233 223 197 SnaBI TAC'GTA Cuts at: 493 4530 493 Size: 4037 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6739 SphI G_CATG'C Cuts at: 1132 4097 1132 Size: 2965 3774 SspI AAT'ATT Cuts at: 5 52 2036 4663 5 Size: 47 1984 2627 2081 Fragments arranged by size: 2627 2081 1984 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6739 StyI C'CwwG_G Cuts at: 513 1065 2004 2218 2919 3126 4178 513 Size: 552 939 214 701 207 1052 3074 Fragments arranged by size: 3074 1052 939 701 552 214 207 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4766 4783 4936 5121 5566 325 Size: 1255 3186 17 153 185 445 1498 Fragments arranged by size: 3186 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4985 6697 96 Size: 274 80 33 51 1443 3008 1712 138 Fragments arranged by size: 3008 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6699 6731 6699 Size: 32 6707 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6739 XbaI T'CTAG_A Cuts at: 4075 4075 Size: 6739 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2602 3820 2602 Size: 1218 5521 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6739 XmaI C'CCGG_G Cuts at: 1870 4064 1870 Size: 2194 4545 XmnI GAAnn'nnTTC Cuts at: 2213 4868 2213 Size: 2655 4084 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2299 4547 276 Size: 53 83 186 936 765 2248 2468 Fragments arranged by size: 2468 2248 936 765 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI