(Circular) (Six-base) MAPSORT of: pFN21AB9770.seq Check: 2231 from: 1 to: 6091 pFN21AB9770 6091 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2522 2522 Size: 6091 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3901 278 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2669 3434 1937 Size: 732 765 4594 Fragments arranged by size: 4594 765 732 Acc65I G'GTAC_C Cuts at: 3412 3412 Size: 6091 AclI AA'CG_TT Cuts at: 1563 4218 4591 1563 Size: 2655 373 3063 Fragments arranged by size: 3063 2655 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2248 4154 5538 5806 1632 Size: 616 1906 1384 268 1917 Fragments arranged by size: 1917 1906 1384 616 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5894 AflIII A'CryG_T Cuts at: 1203 4990 5967 1203 Size: 3787 977 1327 Fragments arranged by size: 3787 1327 977 AgeI A'CCGG_T Cuts at: 2559 4922 2559 Size: 2363 3728 AhdI GACnn_n'nnGTC Cuts at: 4820 4820 Size: 6091 AleI CACnn'nnGTG Cuts at: 1166 2045 2840 1166 Size: 879 795 4417 Fragments arranged by size: 4417 879 795 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6091 AlwNI CAG_nnn'CTG Cuts at: 3834 5406 3834 Size: 1572 4519 ApaI G_GGCC'C Cuts at: 2192 2225 2192 Size: 33 6058 ApaLI G'TGCA_C Cuts at: 4148 5304 4148 Size: 1156 4935 ApoI r'AATT_y Cuts at: 1493 3400 3626 3778 5887 1493 Size: 1907 226 152 2109 1697 Fragments arranged by size: 2109 1907 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4645 160 Size: 872 3613 1606 Fragments arranged by size: 3613 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 AvaI C'yCGr_G Cuts at: 1870 1943 2134 3416 1870 Size: 73 191 1282 4545 Fragments arranged by size: 4545 1282 191 73 BamHI G'GATC_C Cuts at: 2706 2859 2973 3421 3788 2706 Size: 153 114 448 367 5009 Fragments arranged by size: 5009 448 367 153 114 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2239 2562 3032 3373 3412 Size: 325 217 627 452 323 470 341 39 Cuts at: 3412 4867 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 470 452 341 325 323 217 39 BanII G_rGCy'C Cuts at: 1391 2171 2192 2225 2736 3153 3294 3410 1391 Size: 780 21 33 511 417 141 116 4072 Fragments arranged by size: 4072 780 511 417 141 116 33 21 BbeI G_GCGC'C Cuts at: 3036 3377 3036 Size: 341 5750 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5120 BbvCI CC'TCA_GC Cuts at: 3179 3179 Size: 6091 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4282 4316 1903 Size: 34 2345 34 3678 Fragments arranged by size: 3678 2345 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3983 5193 3983 Size: 1210 4881 BclI T'GATC_A Cuts at: 1548 1548 Size: 6091 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2346 4702 136 Size: 107 122 71 631 605 674 2356 1525 Fragments arranged by size: 2356 1525 674 631 605 122 107 71 BglII A'GATC_T Cuts at: 6086 6086 Size: 6091 BlpI GC'TnA_GC Cuts at: 2285 2718 3252 3508 2285 Size: 433 534 256 4868 Fragments arranged by size: 4868 534 433 256 Bme1580I G_kGCm'C Cuts at: 1790 2192 2225 2244 4152 5308 1790 Size: 402 33 19 1908 1156 2573 Fragments arranged by size: 2573 1908 1156 402 33 19 BmgBI CAC'GTC Cuts at: 1318 2998 1318 Size: 1680 4411 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3822 4780 442 Size: 416 53 83 399 24 2405 958 1753 Fragments arranged by size: 2405 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6091 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2732 3389 4751 1239 Size: 1493 657 1362 2579 Fragments arranged by size: 2579 1493 1362 657 Bpu10I CC'TnA_GC Cuts at: 2165 3179 2165 Size: 1014 5077 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2339 4216 5081 5379 2339 Size: 1877 865 298 3051 Fragments arranged by size: 3051 1877 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2738 4754 882 Size: 617 1239 2016 2219 Fragments arranged by size: 2219 2016 1239 617 BsaAI yAC'GTr Cuts at: 493 1204 2752 3882 493 Size: 711 1548 1130 2702 Fragments arranged by size: 2702 1548 1130 711 BsaBI GATnn'nnATC Cuts at: 6085 6085 Size: 6091 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2349 3033 3374 3898 Size: 53 83 186 936 816 684 341 524 Cuts at: 3898 4280 4915 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 816 684 635 524 382 341 186 83 53 BsaWI w'CCGG_w Cuts at: 2559 2646 3003 4524 4922 5196 5343 5754 2559 Size: 87 357 1521 398 274 147 411 2896 Fragments arranged by size: 2896 1521 411 398 357 274 147 87 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3050 3080 3125 3155 1178 Size: 30 165 30 1647 30 45 30 4114 Fragments arranged by size: 4114 1647 165 45 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2044 2419 2639 1186 Size: 858 375 220 4638 Fragments arranged by size: 4638 858 375 220 BseYI C'CCAG_C Cuts at: 2280 2777 3103 5294 5790 2280 Size: 497 326 2191 496 2581 Fragments arranged by size: 2581 2191 497 496 326 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2087 2639 2978 1163 Size: 573 351 552 339 4276 Fragments arranged by size: 4276 573 552 351 339 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3165 3541 4302 5330 1540 Size: 275 186 1164 376 761 1028 2301 Fragments arranged by size: 2301 1164 1028 761 376 275 186 BsiHKAI G_wGCw'C Cuts at: 2171 4152 4237 5308 2171 Size: 1981 85 1071 2954 Fragments arranged by size: 2954 1981 1071 85 BsmI GAATG_Cn' Cuts at: 2146 3602 3695 2146 Size: 1456 93 4542 Fragments arranged by size: 4542 1456 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2171 2192 2225 2244 2736 3153 3294 Size: 399 381 21 33 19 492 417 141 Cuts at: 3294 3410 4152 4237 5308 1391 Size: 116 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 492 417 399 381 141 116 85 33 21 19 BspEI T'CCGG_A Cuts at: 5754 5754 Size: 6091 BspHI T'CATG_A Cuts at: 3980 5710 3980 Size: 1730 4361 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2522 3446 844 Size: 1678 924 3489 Fragments arranged by size: 3489 1678 924 BsrBI CCG'CTC Cuts at: 1394 1823 2029 2427 2629 2952 3978 1394 Size: 429 206 398 202 323 1026 3507 Fragments arranged by size: 3507 1026 429 398 323 206 202 BsrDI GCAATG_nn' Cuts at: 65 1248 4586 4760 65 Size: 1183 3338 174 1396 Fragments arranged by size: 3338 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2559 2695 3271 4735 4922 1700 Size: 110 749 136 576 1464 187 2869 Fragments arranged by size: 2869 1464 749 576 187 136 110 BsrGI T'GTAC_A Cuts at: 96 2451 96 Size: 2355 3736 BssHII G'CGCG_C Cuts at: 1925 3115 1925 Size: 1190 4901 BssSI C'ACGA_G Cuts at: 4151 5163 4151 Size: 1012 5079 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6091 BstBI TT'CG_AA Cuts at: 4895 4895 Size: 6091 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6091 BstYI r'GATC_y Cuts at: 1972 2706 2859 2973 3421 3788 4173 4190 5642 Size: 734 153 114 448 367 385 17 1452 Cuts at: 5642 5728 5740 6086 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 734 448 385 367 346 153 114 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2374 2431 2374 Size: 57 6034 BtgI C'CryG_G Cuts at: 513 1065 2004 2085 2217 2595 2727 513 Size: 552 939 81 132 378 132 3877 Fragments arranged by size: 3877 939 552 378 132 132 81 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2917 2941 487 Size: 683 56 1691 24 3637 Fragments arranged by size: 3637 1691 683 56 24 BtsI GCAGTG_nn' Cuts at: 3607 4401 4421 3607 Size: 794 20 5277 Fragments arranged by size: 5277 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6091 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6056 DraI TTT'AAA Cuts at: 3395 3395 Size: 6091 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2894 5098 809 Size: 507 1578 2204 1802 Fragments arranged by size: 2204 1802 1578 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2592 2724 2982 Size: 54 1425 50 275 18 762 132 258 Cuts at: 2982 3162 3538 4427 6056 8 Size: 180 376 889 1629 43 Fragments arranged by size: 1629 1425 889 762 376 275 258 180 132 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3162 3538 1537 Size: 275 1350 376 4090 Fragments arranged by size: 4090 1350 376 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3317 4021 1353 Size: 1964 704 3423 Fragments arranged by size: 3423 1964 704 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3254 4678 5052 5198 387 Size: 1064 138 1665 1424 374 146 1280 Fragments arranged by size: 1665 1424 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2169 2169 Size: 6091 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2248 2732 3389 4154 4751 5538 5806 Size: 393 616 484 657 765 597 787 268 Cuts at: 5806 1239 Size: 1524 Fragments arranged by size: 1524 787 765 657 616 597 484 393 268 EcoO109I rG'GnC_Cy Cuts at: 2117 2222 2359 2500 2117 Size: 105 137 141 5708 Fragments arranged by size: 5708 141 137 105 EcoRI G'AATT_C Cuts at: 3400 3778 3400 Size: 378 5713 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2494 2494 Size: 6091 FspI TGC'GCA Cuts at: 2495 4597 2495 Size: 2102 3989 HaeII r_GCGC'y Cuts at: 1678 2682 3015 3036 3179 3377 5238 1678 Size: 1004 333 21 143 198 1861 2531 Fragments arranged by size: 2531 1861 1004 333 198 143 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2414 2446 2651 2683 3050 Size: 32 135 32 842 32 205 32 367 Cuts at: 3050 3082 4732 4764 4806 4838 1373 Size: 32 1650 32 42 32 2626 Fragments arranged by size: 2626 1650 842 367 205 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2670 3435 3681 669 Size: 988 281 732 765 246 3079 Fragments arranged by size: 3079 988 765 732 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6091 HpaI GTT'AAC Cuts at: 3681 3681 Size: 6091 KasI G'GCGC_C Cuts at: 3032 3373 3032 Size: 341 5750 KpnI G_GTAC'C Cuts at: 3416 3416 Size: 6091 MfeI C'AATT_G Cuts at: 3690 3690 Size: 6091 MluI A'CGCG_T Cuts at: 5967 5967 Size: 6091 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5205 5389 1305 Size: 3900 184 2007 Fragments arranged by size: 3900 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2594 2984 10 Size: 54 1425 343 762 390 3117 Fragments arranged by size: 3117 1425 762 390 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2045 2237 2600 2696 2840 2960 3086 Size: 648 879 192 363 96 144 120 126 Cuts at: 3086 4049 4408 4567 518 Size: 963 359 159 2042 Fragments arranged by size: 2042 963 879 648 363 359 192 159 144 126 120 96 MspA1I CmG'CkG Cuts at: 1606 2143 2457 2554 3507 4184 5332 5577 1606 Size: 537 314 97 953 677 1148 245 2120 Fragments arranged by size: 2120 1148 953 677 537 314 245 97 NaeI GCC'GGC Cuts at: 3273 3273 Size: 6091 NarI GG'CG_CC Cuts at: 3033 3374 3033 Size: 341 5750 NcoI C'CATG_G Cuts at: 513 1065 2004 2085 2217 2595 513 Size: 552 939 81 132 378 4009 Fragments arranged by size: 4009 939 552 378 132 81 NdeI CA'TA_TG Cuts at: 387 387 Size: 6091 NgoMIV G'CCGG_C Cuts at: 3271 3271 Size: 6091 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6091 NotI GC'GGCC_GC Cuts at: 3538 3538 Size: 6091 NspI r_CATG'y Cuts at: 1132 1229 2695 3085 3449 4994 1132 Size: 97 1466 390 364 1545 2229 Fragments arranged by size: 2229 1545 1466 390 364 97 PasI CC'CwG_GG Cuts at: 1348 2113 2257 2335 1348 Size: 765 144 78 5104 Fragments arranged by size: 5104 765 144 78 PciI A'CATG_T Cuts at: 4990 4990 Size: 6091 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6091 PfoI T'CCnGG_A Cuts at: 2709 2709 Size: 6091 PmeI GTTT'AAAC Cuts at: 3395 3395 Size: 6091 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2765 2797 4160 4192 5673 5705 2765 Size: 32 1363 32 1481 32 3151 Fragments arranged by size: 3151 1481 1363 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2359 2500 2359 Size: 141 5950 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6091 PsiI TTA'TAA Cuts at: 3661 3661 Size: 6091 PspOMI G'GGCC_C Cuts at: 2188 2221 2188 Size: 33 6058 PstI C_TGCA'G Cuts at: 830 3443 830 Size: 2613 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6091 PvuII CAG'CTG Cuts at: 2143 2457 2143 Size: 314 5777 SacI G_AGCT'C Cuts at: 2171 2171 Size: 6091 SalI G'TCGA_C Cuts at: 1936 2668 3433 1936 Size: 732 765 4594 Fragments arranged by size: 4594 765 732 SbfI CC_TGCA'GG Cuts at: 3443 3443 Size: 6091 ScaI AGT'ACT Cuts at: 2570 4339 6051 2570 Size: 1769 1712 2610 Fragments arranged by size: 2610 1769 1712 SexAI A'CCwGG_T Cuts at: 2892 2892 Size: 6091 SfcI C'TryA_G Cuts at: 826 1045 3439 4574 5255 5446 826 Size: 219 2394 1135 681 191 1471 Fragments arranged by size: 2394 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 3034 3375 3034 Size: 341 5750 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6091 SmaI CCC'GGG Cuts at: 1872 2136 3418 1872 Size: 264 1282 4545 Fragments arranged by size: 4545 1282 264 SmlI C'TyrA_G Cuts at: 820 1017 1943 2354 4195 5096 5358 820 Size: 197 926 411 1841 901 262 1553 Fragments arranged by size: 1841 1553 926 901 411 262 197 SnaBI TAC'GTA Cuts at: 493 3882 493 Size: 3389 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6091 SphI G_CATG'C Cuts at: 1132 3449 1132 Size: 2317 3774 SspI AAT'ATT Cuts at: 5 52 4015 5 Size: 47 3963 2081 Fragments arranged by size: 3963 2081 47 StuI AGG'CCT Cuts at: 1517 3209 1517 Size: 1692 4399 StyI C'CwwG_G Cuts at: 513 1065 2004 2085 2217 2595 3144 3201 3530 Size: 552 939 81 132 378 549 57 329 Cuts at: 3530 513 Size: 3074 Fragments arranged by size: 3074 939 552 549 378 329 132 81 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2057 2191 2266 2756 3355 4118 4135 Size: 1255 477 134 75 490 599 763 17 Cuts at: 4135 4288 4473 4918 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 763 599 490 477 445 185 153 134 75 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2451 2568 4337 Size: 274 80 33 51 1443 474 117 1769 Cuts at: 4337 6049 96 Size: 1712 138 Fragments arranged by size: 1769 1712 1443 474 274 138 117 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6051 6083 6051 Size: 32 6059 Tth111I GACn'n_nGTC Cuts at: 1316 2795 2894 1316 Size: 1479 99 4513 Fragments arranged by size: 4513 1479 99 XbaI T'CTAG_A Cuts at: 3427 3427 Size: 6091 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2182 2254 2182 Size: 72 6019 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6091 XmaI C'CCGG_G Cuts at: 1870 2134 3416 1870 Size: 264 1282 4545 Fragments arranged by size: 4545 1282 264 XmnI GAAnn'nnTTC Cuts at: 4220 4220 Size: 6091 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3899 276 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI