Restriction map for
pFN21AB9769
(Circular) (Six-base) MAPSORT of: pFN21AB9769.seq Check: 9934 from: 1 to: 7846
pFN21AB9769 7846 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5656 278
Size: 53 83 186 936 4120 2468
Fragments arranged by size:
4120 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4584 5189 1937
Size: 2647 605 4594
Fragments arranged by size:
4594 2647 605
Acc65I G'GTAC_C
Cuts at: 5167 5167
Size: 7846
AclI AA'CG_TT
Cuts at: 1563 2094 3895 5973 6346 1563
Size: 531 1801 2078 373 3063
Fragments arranged by size:
3063 2078 1801 531 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2598 2963 3137 3795 3842 3936 4439 4580
Size: 966 365 174 658 47 94 503 141
Cuts at: 4580 4827 4839 5909 7293 7561 1632
Size: 247 12 1070 1384 268 1917
Fragments arranged by size:
1917 1384 1070 966 658 503 365 268
247 174 141 94 47 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7649
AflIII A'CryG_T
Cuts at: 1203 2976 6745 7722 1203
Size: 1773 3769 977 1327
Fragments arranged by size:
3769 1773 1327 977
AgeI A'CCGG_T
Cuts at: 6677 6677
Size: 7846
AhdI GACnn_n'nnGTC
Cuts at: 6575 6575
Size: 7846
AleI CACnn'nnGTG
Cuts at: 1166 3263 4214 4232 1166
Size: 2097 951 18 4780
Fragments arranged by size:
4780 2097 951 18
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7846
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4205 4237 4205
Size: 32 7814
AlwNI CAG_nnn'CTG
Cuts at: 3251 4826 5589 7161 3251
Size: 1575 763 1572 3936
Fragments arranged by size:
3936 1575 1572 763
ApaLI G'TGCA_C
Cuts at: 5075 5903 7059 5075
Size: 828 1156 5862
Fragments arranged by size:
5862 1156 828
ApoI r'AATT_y
Cuts at: 1493 2426 2591 3068 4348 4391 4663 5155 5381
Size: 933 165 477 1280 43 272 492 226
Cuts at: 5381 5533 7642 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1280 933 492 477 272 226
165 152 43
AseI AT'TA_AT
Cuts at: 160 1032 6400 160
Size: 872 5368 1606
Fragments arranged by size:
5368 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7846
AvaI C'yCGr_G
Cuts at: 1870 1943 3369 3906 5171 1870
Size: 73 1426 537 1265 4545
Fragments arranged by size:
4545 1426 1265 537 73
AvrII C'CTAG_G
Cuts at: 3046 3046
Size: 7846
BamHI G'GATC_C
Cuts at: 5176 5543 5176
Size: 367 7479
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3823 5167 6622 618
Size: 325 217 627 2036 1344 1455 1842
Fragments arranged by size:
2036 1842 1455 1344 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2305 2544 3083 4432 5165 1391
Size: 914 239 539 1349 733 4072
Fragments arranged by size:
4072 1349 914 733 539 239
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2575 4730 928
Size: 971 676 2155 4044
Fragments arranged by size:
4044 2155 971 676
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6037 6071 1903
Size: 34 4100 34 3678
Fragments arranged by size:
4100 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5738 6948 5738
Size: 1210 6636
BclI T'GATC_A
Cuts at: 1548 3960 4614 1548
Size: 2412 654 4780
Fragments arranged by size:
4780 2412 654
BfrBI ATG'CAT
Cuts at: 4276 4276
Size: 7846
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6457 136
Size: 107 122 71 631 605 4785 1525
Fragments arranged by size:
4785 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3588 3994 7841 3588
Size: 406 3847 3593
Fragments arranged by size:
3847 3593 406
BlpI GC'TnA_GC
Cuts at: 4299 5263 4299
Size: 964 6882
Bme1580I G_kGCm'C
Cuts at: 1790 5079 5907 7063 1790
Size: 3289 828 1156 2573
Fragments arranged by size:
3289 2573 1156 828
BmgBI CAC'GTC
Cuts at: 1318 2977 2998 1318
Size: 1659 21 6166
Fragments arranged by size:
6166 1659 21
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5577 6535 442
Size: 416 53 83 399 24 4160 958 1753
Fragments arranged by size:
4160 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7846
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4307 4307
Size: 7846
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2454 2906 4064 4799 5003 6506 1239
Size: 1215 452 1158 735 204 1503 2579
Fragments arranged by size:
2579 1503 1215 1158 735 452 204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2753 2874 4160 5971 6836 7134 2753
Size: 121 1286 1811 865 298 3465
Fragments arranged by size:
3465 1811 1286 865 298 121
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6509 882
Size: 617 5010 2219
Fragments arranged by size:
5010 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5637 493
Size: 711 4433 2702
Fragments arranged by size:
4433 2702 711
BsaBI GATnn'nnATC
Cuts at: 3593 7840 3593
Size: 4247 3599
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5653 6035 6670 275
Size: 53 83 186 936 4120 382 635 1451
Fragments arranged by size:
4120 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3972 6279 6677 6951 7098 7509 3972
Size: 2307 398 274 147 411 4309
Fragments arranged by size:
4309 2307 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4077 4107 1178
Size: 30 165 30 2674 30 4917
Fragments arranged by size:
4917 2674 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2948 3338 3341 3344 4098 4895 1186
Size: 1762 390 3 3 754 797 4137
Fragments arranged by size:
4137 1762 797 754 390 3 3
BseYI C'CCAG_C
Cuts at: 2699 3244 7049 7545 2699
Size: 545 3805 496 3000
Fragments arranged by size:
3805 3000 545 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2520 4148 4238 5106 1163
Size: 573 784 1628 90 868 3903
Fragments arranged by size:
3903 1628 868 784 573 90
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5296 6057 7085 1540
Size: 275 186 3295 761 1028 2301
Fragments arranged by size:
3295 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2544 3433 3739 4432 5079 5907 5992 7063 2544
Size: 889 306 693 647 828 85 1071 3327
Fragments arranged by size:
3327 1071 889 828 693 647 306 85
BsmI GAATG_Cn'
Cuts at: 2296 3868 4186 5357 5450 2296
Size: 1572 318 1171 93 4692
Fragments arranged by size:
4692 1572 1171 318 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2305 2544 3083 3433 3739 4432 5079
Size: 399 515 239 539 350 306 693 647
Cuts at: 5079 5165 5907 5992 7063 1391
Size: 86 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 693 647 539 515 399
350 306 239 86 85
BspEI T'CCGG_A
Cuts at: 7509 7509
Size: 7846
BspHI T'CATG_A
Cuts at: 2442 4719 5735 7465 2442
Size: 2277 1016 1730 2823
Fragments arranged by size:
2823 2277 1730 1016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4454 5201 844
Size: 3610 747 3489
Fragments arranged by size:
3610 3489 747
BsrBI CCG'CTC
Cuts at: 1394 1823 4543 5733 1394
Size: 429 2720 1190 3507
Fragments arranged by size:
3507 2720 1190 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2290 3636 3654 6341 6515 65
Size: 1183 1042 1346 18 2687 174 1396
Fragments arranged by size:
2687 1396 1346 1183 1042 174 18
BsrFI r'CCGG_y
Cuts at: 1700 1810 6490 6677 1700
Size: 110 4680 187 2869
Fragments arranged by size:
4680 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2055 4104 96
Size: 1959 2049 3838
Fragments arranged by size:
3838 2049 1959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7846
BssSI C'ACGA_G
Cuts at: 5906 6918 5906
Size: 1012 6834
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7846
BstBI TT'CG_AA
Cuts at: 4755 6650 4755
Size: 1895 5951
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7846
BstXI CCAn_nnnn'nTGG
Cuts at: 4058 4255 4058
Size: 197 7649
BstYI r'GATC_y
Cuts at: 1972 3588 3994 5176 5543 5928 5945 7397 7483
Size: 1616 406 1182 367 385 17 1452 86
Cuts at: 7483 7495 7841 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1616 1452 1182 406 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3561 4743 4785 3561
Size: 1182 42 6622
Fragments arranged by size:
6622 1182 42
BtgI C'CryG_G
Cuts at: 513 1065 2004 2826 3684 4248 5089 5101 513
Size: 552 939 822 858 564 841 12 3258
Fragments arranged by size:
3258 939 858 841 822 564 552 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2503 487
Size: 683 56 1277 5830
Fragments arranged by size:
5830 1277 683 56
BtsI GCAGTG_nn'
Cuts at: 2315 3739 5362 6156 6176 2315
Size: 1424 1623 794 20 3985
Fragments arranged by size:
3985 1623 1424 794 20
ClaI AT'CG_AT
Cuts at: 1554 3597 1554
Size: 2043 5803
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7811
DraI TTT'AAA
Cuts at: 4345 5150 4345
Size: 805 7041
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6853 809
Size: 507 5537 1802
Fragments arranged by size:
5537 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2232 4493 5293
Size: 54 1425 50 275 18 402 2261 800
Cuts at: 5293 6182 7811 8
Size: 889 1629 43
Fragments arranged by size:
2261 1629 1425 889 800 402 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5293 1537
Size: 275 3481 4090
Fragments arranged by size:
4090 3481 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2042 2441 2676 3183 3315 3355 3411 3421
Size: 689 399 235 507 132 40 56 10
Cuts at: 3421 3912 4866 4872 4911 4950 5054 5776 1353
Size: 491 954 6 39 39 104 722 3423
Fragments arranged by size:
3423 954 722 689 507 491 399 235
132 104 56 40 39 39 10 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2083 6433 6807 6953 387
Size: 1064 138 494 4350 374 146 1280
Fragments arranged by size:
4350 1280 1064 494 374 146 138
EcoICRI GAG'CTC
Cuts at: 2542 4430 2542
Size: 1888 5958
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2454 2598 2906 2963 3137 3795 3842
Size: 393 822 144 308 57 174 658 47
Cuts at: 3842 3936 4064 4439 4580 4799 4827 4839 5003
Size: 94 128 375 141 219 28 12 164
Cuts at: 5003 5909 6506 7293 7561 1239
Size: 906 597 787 268 1524
Fragments arranged by size:
1524 906 822 787 658 597 393 375
308 268 219 174 164 144 141 128
94 57 47 28 12
EcoO109I rG'GnC_Cy
Cuts at: 3349 3349
Size: 7846
EcoRI G'AATT_C
Cuts at: 3068 4663 5155 5533 3068
Size: 1595 492 378 5381
Fragments arranged by size:
5381 1595 492 378
EcoRV GAT'ATC
Cuts at: 2494 2494
Size: 7846
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2371 2371
Size: 7846
FspI TGC'GCA
Cuts at: 6352 6352
Size: 7846
HaeII r_GCGC'y
Cuts at: 1678 6993 1678
Size: 5315 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2866 2898 4808 4834 4840
Size: 32 135 32 1294 32 1910 26 6
Cuts at: 4840 4866 4889 4921 6487 6519 6561 6593 1373
Size: 26 23 32 1566 32 42 32 2626
Fragments arranged by size:
2626 1910 1566 1294 135 42 32 32
32 32 32 32 26 26 23 6
HincII GTy'rAC
Cuts at: 669 1657 1938 5190 5436 669
Size: 988 281 3252 246 3079
Fragments arranged by size:
3252 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7846
HpaI GTT'AAC
Cuts at: 5436 5436
Size: 7846
KpnI G_GTAC'C
Cuts at: 5171 5171
Size: 7846
MfeI C'AATT_G
Cuts at: 2062 5445 2062
Size: 3383 4463
MluI A'CGCG_T
Cuts at: 7722 7722
Size: 7846
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2880 6960 7144 1305
Size: 1575 4080 184 2007
Fragments arranged by size:
4080 2007 1575 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2234 4495 10
Size: 54 1425 343 402 2261 3361
Fragments arranged by size:
3361 2261 1425 402 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2228 2873 2996 3263 4056 4212
Size: 648 843 219 645 123 267 793 156
Cuts at: 4212 4214 4232 4275 5804 6163 6322 518
Size: 2 18 43 1529 359 159 2042
Fragments arranged by size:
2042 1529 843 793 648 645 359 267
219 159 156 123 43 18 2
MspA1I CmG'CkG
Cuts at: 1606 5262 5939 7087 7332 1606
Size: 3656 677 1148 245 2120
Fragments arranged by size:
3656 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2826 513
Size: 552 939 822 5533
Fragments arranged by size:
5533 939 822 552
NdeI CA'TA_TG
Cuts at: 387 2799 4272 387
Size: 2412 1473 3961
Fragments arranged by size:
3961 2412 1473
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7846
NotI GC'GGCC_GC
Cuts at: 5293 5293
Size: 7846
NsiI A_TGCA'T
Cuts at: 4278 4278
Size: 7846
NspI r_CATG'y
Cuts at: 1132 1229 4124 5204 6749 1132
Size: 97 2895 1080 1545 2229
Fragments arranged by size:
2895 2229 1545 1080 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7846
PciI A'CATG_T
Cuts at: 6745 6745
Size: 7846
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7846
PfoI T'CCnGG_A
Cuts at: 4776 4776
Size: 7846
PmeI GTTT'AAAC
Cuts at: 5150 5150
Size: 7846
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5915 5947 7428 7460 5915
Size: 32 1481 32 6301
Fragments arranged by size:
6301 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3349 3349
Size: 7846
PshAI GACnn'nnGTC
Cuts at: 1613 3785 1613
Size: 2172 5674
PsiI TTA'TAA
Cuts at: 2910 3900 5416 2910
Size: 990 1516 5340
Fragments arranged by size:
5340 1516 990
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3825 3857 3825
Size: 32 7814
PstI C_TGCA'G
Cuts at: 830 3415 5198 830
Size: 2585 1783 3478
Fragments arranged by size:
3478 2585 1783
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7846
RsrII CG'GwC_CG
Cuts at: 5005 5005
Size: 7846
SacI G_AGCT'C
Cuts at: 2544 4432 2544
Size: 1888 5958
SalI G'TCGA_C
Cuts at: 1936 5188 1936
Size: 3252 4594
SapI GCTCTTCn'nnn_
Cuts at: 2441 3421 2441
Size: 980 6866
SbfI CC_TGCA'GG
Cuts at: 5198 5198
Size: 7846
ScaI AGT'ACT
Cuts at: 6094 7806 6094
Size: 1712 6134
SfcI C'TryA_G
Cuts at: 826 1045 3411 4435 4594 4629 5194 6329 7010
Size: 219 2366 1024 159 35 565 1135 681
Cuts at: 7010 7201 826
Size: 191 1471
Fragments arranged by size:
2366 1471 1135 1024 681 565 219 191
159 35
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7846
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7846
SmaI CCC'GGG
Cuts at: 1872 5173 1872
Size: 3301 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2768 2889 3906 4175 5950 6851
Size: 197 926 825 121 1017 269 1775 901
Cuts at: 6851 7113 820
Size: 262 1553
Fragments arranged by size:
1775 1553 1017 926 901 825 269 262
197 121
SnaBI TAC'GTA
Cuts at: 493 5637 493
Size: 5144 2702
SpeI A'CTAG_T
Cuts at: 152 4463 152
Size: 4311 3535
SphI G_CATG'C
Cuts at: 1132 4124 5204 1132
Size: 2992 1080 3774
Fragments arranged by size:
3774 2992 1080
SspI AAT'ATT
Cuts at: 5 52 3817 4015 4114 5770 5
Size: 47 3765 198 99 1656 2081
Fragments arranged by size:
3765 2081 1656 198 99 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7846
StyI C'CwwG_G
Cuts at: 513 1065 2004 2826 3046 5285 513
Size: 552 939 822 220 2239 3074
Fragments arranged by size:
3074 2239 939 822 552 220
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2108 4296 5022 5873 5890 6043 6228
Size: 1255 528 2188 726 851 17 153 185
Cuts at: 6228 6673 325
Size: 445 1498
Fragments arranged by size:
2188 1498 1255 851 726 528 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2055 3886 4104
Size: 274 80 33 51 1443 78 1831 218
Cuts at: 4104 4693 6092 7804 96
Size: 589 1399 1712 138
Fragments arranged by size:
1831 1712 1443 1399 589 274 218 138
80 78 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4077 4109 7806 7838 4077
Size: 32 3697 32 4085
Fragments arranged by size:
4085 3697 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7846
XbaI T'CTAG_A
Cuts at: 5182 5182
Size: 7846
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4139 4139
Size: 7846
XhoI C'TCGA_G
Cuts at: 1943 3906 1943
Size: 1963 5883
XmaI C'CCGG_G
Cuts at: 1870 5171 1870
Size: 3301 4545
XmnI GAAnn'nnTTC
Cuts at: 3364 3866 5975 3364
Size: 502 2109 5235
Fragments arranged by size:
5235 2109 502
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5654 276
Size: 53 83 186 936 4120 2468
Fragments arranged by size:
4120 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI RsrII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoNI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI
PvuII SacII SanDI SexAI SfiI SfoI SrfI SwaI