(Circular) (Six-base) MAPSORT of: pFN21AB9767.seq Check: 9257 from: 1 to: 7546 pFN21AB9767 7546 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5356 278 Size: 53 83 186 936 3820 2468 Fragments arranged by size: 3820 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2020 4889 1937 Size: 83 2869 4594 Fragments arranged by size: 4594 2869 83 Acc65I G'GTAC_C Cuts at: 4787 4867 4787 Size: 80 7466 AclI AA'CG_TT Cuts at: 1563 5673 6046 1563 Size: 4110 373 3063 Fragments arranged by size: 4110 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2084 2262 2574 2655 3083 3246 3266 3270 Size: 452 178 312 81 428 163 20 4 Cuts at: 3270 3528 3966 4116 5609 6993 7261 1632 Size: 258 438 150 1493 1384 268 1917 Fragments arranged by size: 1917 1493 1384 452 438 428 312 268 258 178 163 150 81 20 4 AfeI AGC'GCT Cuts at: 2249 2249 Size: 7546 AflII C'TTAA_G Cuts at: 820 1017 3973 820 Size: 197 2956 4393 Fragments arranged by size: 4393 2956 197 AflIII A'CryG_T Cuts at: 1203 4186 6445 7422 1203 Size: 2983 2259 977 1327 Fragments arranged by size: 2983 2259 1327 977 AgeI A'CCGG_T Cuts at: 6377 6377 Size: 7546 AhdI GACnn_n'nnGTC Cuts at: 6275 6275 Size: 7546 AleI CACnn'nnGTG Cuts at: 1166 2832 1166 Size: 1666 5880 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7546 AlwNI CAG_nnn'CTG Cuts at: 3953 5289 6861 3953 Size: 1336 1572 4638 Fragments arranged by size: 4638 1572 1336 ApaLI G'TGCA_C Cuts at: 5603 6759 5603 Size: 1156 6390 ApoI r'AATT_y Cuts at: 1493 2532 2693 3011 3844 4275 4855 5081 5233 Size: 1039 161 318 833 431 580 226 152 Cuts at: 5233 7342 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1039 833 580 431 318 226 161 152 AseI AT'TA_AT Cuts at: 160 1032 3032 6100 160 Size: 872 2000 3068 1606 Fragments arranged by size: 3068 2000 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7546 AvaI C'yCGr_G Cuts at: 1870 1943 4871 1870 Size: 73 2928 4545 Fragments arranged by size: 4545 2928 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2087 2120 2087 Size: 33 7513 BamHI G'GATC_C Cuts at: 4876 5243 4876 Size: 367 7179 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4787 4867 6322 618 Size: 325 217 627 3000 80 1455 1842 Fragments arranged by size: 3000 1842 1455 627 325 217 80 BanII G_rGCy'C Cuts at: 1391 4479 4865 1391 Size: 3088 386 4072 Fragments arranged by size: 4072 3088 386 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2298 2804 928 Size: 971 399 506 5670 Fragments arranged by size: 5670 971 506 399 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5737 5771 1903 Size: 34 3800 34 3678 Fragments arranged by size: 3800 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5438 6648 5438 Size: 1210 6336 BclI T'GATC_A Cuts at: 1548 1548 Size: 7546 BfrBI ATG'CAT Cuts at: 2879 3005 4086 4330 2879 Size: 126 1081 244 6095 Fragments arranged by size: 6095 1081 244 126 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6157 136 Size: 107 122 71 631 605 4485 1525 Fragments arranged by size: 4485 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7541 7541 Size: 7546 BlpI GC'TnA_GC Cuts at: 4963 4963 Size: 7546 Bme1580I G_kGCm'C Cuts at: 1790 5607 6763 1790 Size: 3817 1156 2573 Fragments arranged by size: 3817 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7546 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5277 6235 442 Size: 416 53 83 399 24 3860 958 1753 Fragments arranged by size: 3860 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7546 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2358 2584 3048 3299 3603 4680 6206 1239 Size: 1119 226 464 251 304 1077 1526 2579 Fragments arranged by size: 2579 1526 1119 1077 464 304 251 226 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4569 4683 5671 6536 6834 4569 Size: 114 988 865 298 5281 Fragments arranged by size: 5281 988 865 298 114 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3730 6209 882 Size: 617 2231 2479 2219 Fragments arranged by size: 2479 2231 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5337 493 Size: 711 4133 2702 Fragments arranged by size: 4133 2702 711 BsaBI GATnn'nnATC Cuts at: 7540 7540 Size: 7546 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4110 5353 5735 6370 Size: 53 83 186 936 2577 1243 382 635 Cuts at: 6370 275 Size: 1451 Fragments arranged by size: 2577 1451 1243 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5979 6377 6651 6798 7209 5979 Size: 398 274 147 411 6316 Fragments arranged by size: 6316 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2577 2607 2644 2674 1178 Size: 30 165 30 1174 30 37 30 6050 Fragments arranged by size: 6050 1174 165 37 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2037 3211 4777 4780 4783 1186 Size: 851 1174 1566 3 3 3949 Fragments arranged by size: 3949 1566 1174 851 3 3 BseYI C'CCAG_C Cuts at: 2498 6749 7245 2498 Size: 4251 496 2799 Fragments arranged by size: 4251 2799 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6973 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4996 5757 6785 1540 Size: 275 186 2995 761 1028 2301 Fragments arranged by size: 2995 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4479 4658 5607 5692 6763 4479 Size: 179 949 85 1071 5262 Fragments arranged by size: 5262 1071 949 179 85 BsmI GAATG_Cn' Cuts at: 3099 4088 4414 5057 5150 3099 Size: 989 326 643 93 5495 Fragments arranged by size: 5495 989 643 326 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4479 4658 4865 5607 5692 6763 1391 Size: 399 2689 179 207 742 85 1071 2174 Fragments arranged by size: 2689 2174 1071 742 399 207 179 85 BspEI T'CCGG_A Cuts at: 7209 7209 Size: 7546 BspHI T'CATG_A Cuts at: 3039 5435 7165 3039 Size: 2396 1730 3420 Fragments arranged by size: 3420 2396 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4901 844 Size: 4057 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5433 1394 Size: 429 3610 3507 Fragments arranged by size: 3610 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3412 4785 6041 6215 65 Size: 1183 2164 1373 1256 174 1396 Fragments arranged by size: 2164 1396 1373 1256 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6190 6377 1700 Size: 110 4380 187 2869 Fragments arranged by size: 4380 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3301 4384 96 Size: 3205 1083 3258 Fragments arranged by size: 3258 3205 1083 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7546 BssSI C'ACGA_G Cuts at: 5606 6618 5606 Size: 1012 6534 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7546 BstBI TT'CG_AA Cuts at: 3111 6350 3111 Size: 3239 4307 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7546 BstYI r'GATC_y Cuts at: 1972 2956 3129 4600 4876 5243 5628 5645 7097 Size: 984 173 1471 276 367 385 17 1452 Cuts at: 7097 7183 7195 7541 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1471 1452 984 385 367 346 276 173 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4288 4288 Size: 7546 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6994 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6807 Fragments arranged by size: 6807 683 56 BtsI GCAGTG_nn' Cuts at: 2726 3973 3997 5062 5856 5876 2726 Size: 1247 24 1065 794 20 4396 Fragments arranged by size: 4396 1247 1065 794 24 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7546 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7511 DraI TTT'AAA Cuts at: 2232 4850 2232 Size: 2618 4928 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6553 809 Size: 507 5237 1802 Fragments arranged by size: 5237 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4993 5882 7511 Size: 54 1425 50 275 18 3163 889 1629 Cuts at: 7511 8 Size: 43 Fragments arranged by size: 3163 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4993 1537 Size: 275 3181 4090 Fragments arranged by size: 4090 3181 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2406 2633 3241 3985 4484 4659 5476 1353 Size: 1053 227 608 744 499 175 817 3423 Fragments arranged by size: 3423 1053 817 744 608 499 227 175 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6133 6507 6653 387 Size: 1064 138 4544 374 146 1280 Fragments arranged by size: 4544 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4477 4477 Size: 7546 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2262 2358 2574 2584 2655 3048 Size: 393 452 178 96 216 10 71 393 Cuts at: 3048 3083 3246 3266 3270 3299 3528 3603 3966 Size: 35 163 20 4 29 229 75 363 Cuts at: 3966 4116 4680 5609 6206 6993 7261 1239 Size: 150 564 929 597 787 268 1524 Fragments arranged by size: 1524 929 787 597 564 452 393 393 363 268 229 216 178 163 150 96 75 71 35 29 20 10 4 EcoNI CCTnn'n_nnAGG Cuts at: 4287 4287 Size: 7546 EcoRI G'AATT_C Cuts at: 4855 5233 4855 Size: 378 7168 EcoRV GAT'ATC Cuts at: 3181 3181 Size: 7546 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2266 2870 3645 3800 2266 Size: 604 775 155 6012 Fragments arranged by size: 6012 775 604 155 FspI TGC'GCA Cuts at: 6052 6052 Size: 7546 HaeII r_GCGC'y Cuts at: 1678 2251 6693 1678 Size: 573 4442 2531 Fragments arranged by size: 4442 2531 573 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2577 2609 3962 3986 3994 Size: 32 135 32 1005 32 1353 24 8 Cuts at: 3994 4018 4130 4162 6187 6219 6261 6293 1373 Size: 24 112 32 2025 32 42 32 2626 Fragments arranged by size: 2626 2025 1353 1005 135 112 42 32 32 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 2021 2041 2596 2902 4890 5136 Size: 988 281 83 20 555 306 1988 246 Cuts at: 5136 669 Size: 3079 Fragments arranged by size: 3079 1988 988 555 306 281 246 83 20 HindIII A'AGCT_T Cuts at: 748 3278 748 Size: 2530 5016 HpaI GTT'AAC Cuts at: 2041 5136 2041 Size: 3095 4451 KpnI G_GTAC'C Cuts at: 4791 4871 4791 Size: 80 7466 MfeI C'AATT_G Cuts at: 5145 5145 Size: 7546 MluI A'CGCG_T Cuts at: 7422 7422 Size: 7546 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2726 6660 6844 1305 Size: 1421 3934 184 2007 Fragments arranged by size: 3934 2007 1421 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5724 Fragments arranged by size: 5724 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2832 2878 4322 4355 4796 5504 5863 Size: 648 1666 46 1444 33 441 708 359 Cuts at: 5863 6022 518 Size: 159 2042 Fragments arranged by size: 2042 1666 1444 708 648 441 359 159 46 33 MspA1I CmG'CkG Cuts at: 1606 4962 5639 6787 7032 1606 Size: 3356 677 1148 245 2120 Fragments arranged by size: 3356 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6994 NdeI CA'TA_TG Cuts at: 387 2875 387 Size: 2488 5058 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7546 NotI GC'GGCC_GC Cuts at: 4993 4993 Size: 7546 NsiI A_TGCA'T Cuts at: 2881 3007 4088 4332 2881 Size: 126 1081 244 6095 Fragments arranged by size: 6095 1081 244 126 NspI r_CATG'y Cuts at: 1132 1229 3726 4190 4904 6449 1132 Size: 97 2497 464 714 1545 2229 Fragments arranged by size: 2497 2229 1545 714 464 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7546 PciI A'CATG_T Cuts at: 4186 6445 4186 Size: 2259 5287 PflMI CCAn_nnn'nTGG Cuts at: 1108 4716 1108 Size: 3608 3938 PmeI GTTT'AAAC Cuts at: 4850 4850 Size: 7546 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5615 5647 7128 7160 5615 Size: 32 1481 32 6001 Fragments arranged by size: 6001 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7546 PsiI TTA'TAA Cuts at: 2213 2892 5116 2213 Size: 679 2224 4643 Fragments arranged by size: 4643 2224 679 PstI C_TGCA'G Cuts at: 830 4898 830 Size: 4068 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7546 SacI G_AGCT'C Cuts at: 4479 4479 Size: 7546 SalI G'TCGA_C Cuts at: 1936 2019 4888 1936 Size: 83 2869 4594 Fragments arranged by size: 4594 2869 83 SapI GCTCTTCn'nnn_ Cuts at: 2406 3985 4484 4659 2406 Size: 1579 499 175 5293 Fragments arranged by size: 5293 1579 499 175 SbfI CC_TGCA'GG Cuts at: 4898 4898 Size: 7546 ScaI AGT'ACT Cuts at: 5794 7506 5794 Size: 1712 5834 SfcI C'TryA_G Cuts at: 826 1045 2830 4620 4894 6029 6710 6901 826 Size: 219 1785 1790 274 1135 681 191 1471 Fragments arranged by size: 1790 1785 1471 1135 681 274 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7546 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7546 SmaI CCC'GGG Cuts at: 1872 4873 1872 Size: 3001 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3973 4584 4698 5650 6551 6813 Size: 197 926 2030 611 114 952 901 262 Cuts at: 6813 820 Size: 1553 Fragments arranged by size: 2030 1553 952 926 901 611 262 197 114 SnaBI TAC'GTA Cuts at: 493 5337 493 Size: 4844 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7546 SphI G_CATG'C Cuts at: 1132 4904 1132 Size: 3772 3774 SspI AAT'ATT Cuts at: 5 52 2264 5470 5 Size: 47 2212 3206 2081 Fragments arranged by size: 3206 2212 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7546 StyI C'CwwG_G Cuts at: 513 1065 4985 513 Size: 552 3920 3074 Fragments arranged by size: 3920 3074 552 SwaI ATTT'AAAT Cuts at: 2232 2232 Size: 7546 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2414 2512 5573 5590 5743 5928 6373 Size: 1255 834 98 3061 17 153 185 445 Cuts at: 6373 325 Size: 1498 Fragments arranged by size: 3061 1498 1255 834 445 185 153 98 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3054 3301 4384 Size: 274 80 33 51 1443 1077 247 1083 Cuts at: 4384 5792 7504 96 Size: 1408 1712 138 Fragments arranged by size: 1712 1443 1408 1083 1077 274 247 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4116 4148 7506 7538 4116 Size: 32 3358 32 4124 Fragments arranged by size: 4124 3358 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2939 1316 Size: 1623 5923 XbaI T'CTAG_A Cuts at: 2368 3848 4882 2368 Size: 1480 1034 5032 Fragments arranged by size: 5032 1480 1034 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7546 XmaI C'CCGG_G Cuts at: 1870 4871 1870 Size: 3001 4545 XmnI GAAnn'nnTTC Cuts at: 3783 5675 3783 Size: 1892 5654 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5354 276 Size: 53 83 186 936 3820 2468 Fragments arranged by size: 3820 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI XcmI