Restriction map for
pFN21AB9767
(Linear) (Six-base) MAPSORT of: KIBB9767.seq Check: 6330 from: 1 to: 2841
KIBB9767.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AccI GT'mk_AC
Cuts at: 0 15 2841
Size: 15 2826
Acc65I G'GTAC_C
Cuts at: 0 2782 2841
Size: 2782 59
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 257 569 650 1078 1241 1261 1265
Size: 79 178 312 81 428 163 20 4
Cuts at: 1265 1523 1961 2111 2841
Size: 258 438 150 730
Fragments arranged by size:
730 438 428 312 258 178 163 150
81 79 20 4
AfeI AGC'GCT
Cuts at: 0 244 2841
Size: 244 2597
AflII C'TTAA_G
Cuts at: 0 1968 2841
Size: 1968 873
AflIII A'CryG_T
Cuts at: 0 2181 2841
Size: 2181 660
AleI CACnn'nnGTG
Cuts at: 0 827 2841
Size: 827 2014
AlwNI CAG_nnn'CTG
Cuts at: 0 1948 2841
Size: 1948 893
ApoI r'AATT_y
Cuts at: 0 527 688 1006 1839 2270 2841
Size: 527 161 318 833 431 571
Fragments arranged by size:
833 571 527 431 318 161
AseI AT'TA_AT
Cuts at: 0 1027 2841
Size: 1027 1814
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 82 115 2841
Size: 82 33 2726
Fragments arranged by size:
2726 82 33
BanI G'GyrC_C
Cuts at: 0 2782 2841
Size: 2782 59
BanII G_rGCy'C
Cuts at: 0 2474 2841
Size: 2474 367
BbsI GAAGACnn'nnnn_
Cuts at: 0 293 799 2841
Size: 293 506 2042
Fragments arranged by size:
2042 506 293
BfrBI ATG'CAT
Cuts at: 0 874 1000 2081 2325 2841
Size: 874 126 1081 244 516
Fragments arranged by size:
1081 874 516 244 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 579 1043 1294 1598 2675 2841
Size: 353 226 464 251 304 1077 166
Fragments arranged by size:
1077 464 353 304 251 226 166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2564 2678 2841
Size: 2564 114 163
Fragments arranged by size:
2564 163 114
BsaI GGTCTCn'nnnn_
Cuts at: 0 1725 2841
Size: 1725 1116
BsaHI Gr'CG_yC
Cuts at: 0 2105 2841
Size: 2105 736
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 572 602 639 669 2841
Size: 572 30 37 30 2172
Fragments arranged by size:
2172 572 37 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 32 1206 2772 2775 2778 2841
Size: 32 1174 1566 3 3 63
Fragments arranged by size:
1566 1174 63 32 3 3
BseYI C'CCAG_C
Cuts at: 0 493 2841
Size: 493 2348
BsiHKAI G_wGCw'C
Cuts at: 0 2474 2653 2841
Size: 2474 179 188
Fragments arranged by size:
2474 188 179
BsmI GAATG_Cn'
Cuts at: 0 1094 2083 2409 2841
Size: 1094 989 326 432
Fragments arranged by size:
1094 989 432 326
Bsp1286I G_dGCh'C
Cuts at: 0 2474 2653 2841
Size: 2474 179 188
Fragments arranged by size:
2474 188 179
BspHI T'CATG_A
Cuts at: 0 1034 2841
Size: 1034 1807
BsrDI GCAATG_nn'
Cuts at: 0 1407 2780 2841
Size: 1407 1373 61
Fragments arranged by size:
1407 1373 61
BsrGI T'GTAC_A
Cuts at: 0 1296 2379 2841
Size: 1296 1083 462
Fragments arranged by size:
1296 1083 462
BstBI TT'CG_AA
Cuts at: 0 1106 2841
Size: 1106 1735
BstYI r'GATC_y
Cuts at: 0 951 1124 2595 2841
Size: 951 173 1471 246
Fragments arranged by size:
1471 951 246 173
Bsu36I CC'TnA_GG
Cuts at: 0 2283 2841
Size: 2283 558
BtsI GCAGTG_nn'
Cuts at: 0 721 1968 1992 2841
Size: 721 1247 24 849
Fragments arranged by size:
1247 849 721 24
DraI TTT'AAA
Cuts at: 0 227 2841
Size: 227 2614
EarI CTCTTCn'nnn_
Cuts at: 0 401 628 1236 1980 2479 2654 2841
Size: 401 227 608 744 499 175 187
Fragments arranged by size:
744 608 499 401 227 187 175
EcoICRI GAG'CTC
Cuts at: 0 2472 2841
Size: 2472 369
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 257 353 569 579 650 1043 1078
Size: 79 178 96 216 10 71 393 35
Cuts at: 1078 1241 1261 1265 1294 1523 1598 1961 2111
Size: 163 20 4 29 229 75 363 150
Cuts at: 2111 2675 2841
Size: 564 166
Fragments arranged by size:
564 393 363 229 216 178 166 163
150 96 79 75 71 35 29 20
10 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2282 2841
Size: 2282 559
EcoRV GAT'ATC
Cuts at: 0 1176 2841
Size: 1176 1665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 261 865 1640 1795 2841
Size: 261 604 775 155 1046
Fragments arranged by size:
1046 775 604 261 155
HaeII r_GCGC'y
Cuts at: 0 246 2841
Size: 246 2595
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 572 604 1957 1981 1989 2013 2125 2157
Size: 572 32 1353 24 8 24 112 32
Cuts at: 2157 2841
Size: 684
Fragments arranged by size:
1353 684 572 112 32 32 24 24
8
HincII GTy'rAC
Cuts at: 0 16 36 591 897 2841
Size: 16 20 555 306 1944
Fragments arranged by size:
1944 555 306 20 16
HindIII A'AGCT_T
Cuts at: 0 1273 2841
Size: 1273 1568
HpaI GTT'AAC
Cuts at: 0 36 2841
Size: 36 2805
KpnI G_GTAC'C
Cuts at: 0 2786 2841
Size: 2786 55
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 2841
Size: 721 2120
MslI CAynn'nnrTG
Cuts at: 0 827 873 2317 2350 2791 2841
Size: 827 46 1444 33 441 50
Fragments arranged by size:
1444 827 441 50 46 33
NdeI CA'TA_TG
Cuts at: 0 870 2841
Size: 870 1971
NsiI A_TGCA'T
Cuts at: 0 876 1002 2083 2327 2841
Size: 876 126 1081 244 514
Fragments arranged by size:
1081 876 514 244 126
NspI r_CATG'y
Cuts at: 0 1721 2185 2841
Size: 1721 464 656
Fragments arranged by size:
1721 656 464
PciI A'CATG_T
Cuts at: 0 2181 2841
Size: 2181 660
PflMI CCAn_nnn'nTGG
Cuts at: 0 2711 2841
Size: 2711 130
PsiI TTA'TAA
Cuts at: 0 208 887 2841
Size: 208 679 1954
Fragments arranged by size:
1954 679 208
SacI G_AGCT'C
Cuts at: 0 2474 2841
Size: 2474 367
SalI G'TCGA_C
Cuts at: 0 14 2841
Size: 14 2827
SapI GCTCTTCn'nnn_
Cuts at: 0 401 1980 2479 2654 2841
Size: 401 1579 499 175 187
Fragments arranged by size:
1579 499 401 187 175
SfcI C'TryA_G
Cuts at: 0 825 2615 2841
Size: 825 1790 226
Fragments arranged by size:
1790 825 226
SmlI C'TyrA_G
Cuts at: 0 1968 2579 2693 2841
Size: 1968 611 114 148
Fragments arranged by size:
1968 611 148 114
SspI AAT'ATT
Cuts at: 0 259 2841
Size: 259 2582
SwaI ATTT'AAAT
Cuts at: 0 227 2841
Size: 227 2614
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 409 507 2841
Size: 409 98 2334
Fragments arranged by size:
2334 409 98
TatI w'GTAC_w
Cuts at: 0 1049 1296 2379 2841
Size: 1049 247 1083 462
Fragments arranged by size:
1083 1049 462 247
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2111 2143 2841
Size: 2111 32 698
Fragments arranged by size:
2111 698 32
Tth111I GACn'n_nGTC
Cuts at: 0 934 2841
Size: 934 1907
XbaI T'CTAG_A
Cuts at: 0 363 1843 2841
Size: 363 1480 998
Fragments arranged by size:
1480 998 363
XmnI GAAnn'nnTTC
Cuts at: 0 1778 2841
Size: 1778 1063
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflII AflIII AleI AlwNI
ApoI AseI BaeI BanI BanII BbsI BfrBI BpmI
BpuEI BsaI BsaHI BsaXI BseRI BseYI BsiHKAI BsmI
Bsp1286I BspHI BsrDI BsrGI BstBI BstYI Bsu36I BtsI
DraI EarI EcoICRI Eco57MI EcoNI EcoRV FalI HaeII
Hin4I HincII HindIII HpaI KpnI MmeI MslI NdeI
NsiI NspI PciI PflMI PsiI SacI SalI SapI
SfcI SmlI SspI SwaI TaqII TatI TstI Tth111I
XbaI XmnI
Enzymes that do not cut:
AarI AatII AclI AgeI AhdI AlfI AloI ApaI
ApaLI AscI AsiSI AvaI AvrII BamHI BbeI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsgI
BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstEII BstXI BstZ17I BtgI BtgZI ClaI
CspCI DraIII DrdI EaeI EagI EciI EcoO109I EcoRI
FseI FspI FspAI KasI MfeI MluI MscI MspA1I
NaeI NarI NcoI NgoMIV NheI NotI NruI PacI
PasI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacII SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI StyI XcmI XhoI
XmaI ZraI