(Circular) (Six-base) MAPSORT of: pFN21AB9764.seq Check: 2057 from: 1 to: 7222 pFN21AB9764 7222 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5032 278 Size: 53 83 186 936 3496 2468 Fragments arranged by size: 3496 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4565 1937 Size: 2628 4594 Acc65I G'GTAC_C Cuts at: 4543 4543 Size: 7222 AclI AA'CG_TT Cuts at: 1563 2950 5349 5722 1563 Size: 1387 2399 373 3063 Fragments arranged by size: 3063 2399 1387 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2380 2469 2552 3637 5285 6669 6937 1632 Size: 748 89 83 1085 1648 1384 268 1917 Fragments arranged by size: 1917 1648 1384 1085 748 268 89 83 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7025 AflIII A'CryG_T Cuts at: 1203 2903 4467 6121 7098 1203 Size: 1700 1564 1654 977 1327 Fragments arranged by size: 1700 1654 1564 1327 977 AgeI A'CCGG_T Cuts at: 6053 6053 Size: 7222 AhdI GACnn_n'nnGTC Cuts at: 5951 5951 Size: 7222 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7222 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7222 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2123 2155 2123 Size: 32 7190 AlwNI CAG_nnn'CTG Cuts at: 4965 6537 4965 Size: 1572 5650 ApaLI G'TGCA_C Cuts at: 5279 6435 5279 Size: 1156 6066 ApoI r'AATT_y Cuts at: 1493 2051 2288 2613 2821 2989 3015 3073 3099 Size: 558 237 325 208 168 26 58 26 Cuts at: 3099 3241 3267 3577 3603 3745 3771 3855 3997 Size: 142 26 310 26 142 26 84 142 Cuts at: 3997 4249 4275 4359 4516 4531 4757 4909 7018 Size: 252 26 84 157 15 226 152 2109 Cuts at: 7018 1493 Size: 1697 Fragments arranged by size: 2109 1697 558 325 310 252 237 226 208 168 157 152 142 142 142 84 84 58 26 26 26 26 26 26 15 AseI AT'TA_AT Cuts at: 160 1032 5776 160 Size: 872 4744 1606 Fragments arranged by size: 4744 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7222 AvaI C'yCGr_G Cuts at: 1870 1943 4547 1870 Size: 73 2604 4545 Fragments arranged by size: 4545 2604 73 BamHI G'GATC_C Cuts at: 4552 4919 4552 Size: 367 6855 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4543 5998 618 Size: 325 217 627 2756 1455 1842 Fragments arranged by size: 2756 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3157 4541 1391 Size: 1766 1384 4072 Fragments arranged by size: 4072 1766 1384 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3055 3223 3559 3727 3979 4231 928 Size: 971 1156 168 336 168 252 252 3919 Fragments arranged by size: 3919 1156 971 336 252 252 168 168 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5413 5447 1903 Size: 34 3476 34 3678 Fragments arranged by size: 3678 3476 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5114 6324 5114 Size: 1210 6012 BclI T'GATC_A Cuts at: 1548 2268 1548 Size: 720 6502 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5833 136 Size: 107 122 71 631 605 4161 1525 Fragments arranged by size: 4161 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7217 7217 Size: 7222 BlpI GC'TnA_GC Cuts at: 3166 4639 3166 Size: 1473 5749 Bme1580I G_kGCm'C Cuts at: 1790 5283 6439 1790 Size: 3493 1156 2573 Fragments arranged by size: 3493 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2110 1318 Size: 792 6430 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4953 5911 442 Size: 416 53 83 399 24 3536 958 1753 Fragments arranged by size: 3536 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7222 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3045 3129 3283 3297 3465 3549 3633 3717 Size: 1806 84 154 14 168 84 84 84 Cuts at: 3717 3801 3885 4053 4137 4305 5882 1239 Size: 84 84 168 84 168 1577 2579 Fragments arranged by size: 2579 1806 1577 168 168 168 154 84 84 84 84 84 84 84 14 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2447 5347 6212 6510 2447 Size: 2900 865 298 3159 Fragments arranged by size: 3159 2900 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5885 882 Size: 617 4386 2219 Fragments arranged by size: 4386 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5013 493 Size: 711 3809 2702 Fragments arranged by size: 3809 2702 711 BsaBI GATnn'nnATC Cuts at: 7216 7216 Size: 7222 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5029 5411 6046 275 Size: 53 83 186 936 3496 382 635 1451 Fragments arranged by size: 3496 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3359 4367 5655 6053 6327 6474 6885 3359 Size: 1008 1288 398 274 147 411 3696 Fragments arranged by size: 3696 1288 1008 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2123 2153 1178 Size: 30 165 30 720 30 6247 Fragments arranged by size: 6247 720 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 1186 Size: 892 6330 BseYI C'CCAG_C Cuts at: 6425 6921 6425 Size: 496 6726 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6649 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4672 5433 6461 1540 Size: 275 186 2671 761 1028 2301 Fragments arranged by size: 2671 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3157 3493 5283 5368 6439 3157 Size: 336 1790 85 1071 3940 Fragments arranged by size: 3940 1790 1071 336 85 BsmI GAATG_Cn' Cuts at: 3392 4733 4826 3392 Size: 1341 93 5788 Fragments arranged by size: 5788 1341 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3157 3493 4541 5283 5368 6439 1391 Size: 399 1367 336 1048 742 85 1071 2174 Fragments arranged by size: 2174 1367 1071 1048 742 399 336 85 BspEI T'CCGG_A Cuts at: 6885 6885 Size: 7222 BspHI T'CATG_A Cuts at: 3382 5111 6841 3382 Size: 1729 1730 3763 Fragments arranged by size: 3763 1730 1729 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4577 844 Size: 3733 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2087 5109 1394 Size: 429 264 3022 3507 Fragments arranged by size: 3507 3022 429 264 BsrDI GCAATG_nn' Cuts at: 65 1248 4228 5717 5891 65 Size: 1183 2980 1489 174 1396 Fragments arranged by size: 2980 1489 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5866 6053 1700 Size: 110 4056 187 2869 Fragments arranged by size: 4056 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2100 96 Size: 2004 5218 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7222 BssSI C'ACGA_G Cuts at: 5282 6294 5282 Size: 1012 6210 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7222 BstBI TT'CG_AA Cuts at: 6026 6026 Size: 7222 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7222 BstXI CCAn_nnnn'nTGG Cuts at: 2247 4445 2247 Size: 2198 5024 BstYI r'GATC_y Cuts at: 1972 4552 4919 5304 5321 6773 6859 6871 7217 Size: 2580 367 385 17 1452 86 12 346 Cuts at: 7217 1972 Size: 1977 Fragments arranged by size: 2580 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5731 Fragments arranged by size: 5731 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6483 Fragments arranged by size: 6483 683 56 BtsI GCAGTG_nn' Cuts at: 4738 5532 5552 4738 Size: 794 20 6408 Fragments arranged by size: 6408 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7222 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4210 4245 529 Size: 35 3646 35 3506 Fragments arranged by size: 3646 3506 35 35 DraI TTT'AAA Cuts at: 4526 4526 Size: 7222 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6229 809 Size: 507 4913 1802 Fragments arranged by size: 4913 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4669 5558 7187 Size: 54 1425 50 275 18 2839 889 1629 Cuts at: 7187 8 Size: 43 Fragments arranged by size: 2839 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4669 1537 Size: 275 2857 4090 Fragments arranged by size: 4090 2857 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2287 2315 2497 2740 5152 1353 Size: 934 28 182 243 2412 3423 Fragments arranged by size: 3423 2412 934 243 182 28 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5809 6183 6329 387 Size: 1064 138 4220 374 146 1280 Fragments arranged by size: 4220 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3155 3155 Size: 7222 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2380 2469 2552 3045 3129 3283 3297 Size: 393 748 89 83 493 84 154 14 Cuts at: 3297 3465 3549 3633 3637 3717 3801 3885 4053 Size: 168 84 84 4 80 84 84 168 Cuts at: 4053 4137 4305 5285 5882 6669 6937 1239 Size: 84 168 980 597 787 268 1524 Fragments arranged by size: 1524 980 787 748 597 493 393 268 168 168 168 154 89 84 84 84 84 84 84 83 80 14 4 EcoRI G'AATT_C Cuts at: 2051 3073 3099 3267 3603 3745 3771 3855 3997 Size: 1022 26 168 336 142 26 84 142 Cuts at: 3997 4249 4275 4359 4516 4531 4909 2051 Size: 252 26 84 157 15 378 4364 Fragments arranged by size: 4364 1022 378 336 252 168 157 142 142 84 84 26 26 26 15 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2486 2486 Size: 7222 FspI TGC'GCA Cuts at: 5728 5728 Size: 7222 HaeII r_GCGC'y Cuts at: 1678 6369 1678 Size: 4691 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2260 2292 2717 2749 5863 Size: 32 135 32 688 32 425 32 3114 Cuts at: 5863 5895 5937 5969 1373 Size: 32 42 32 2626 Fragments arranged by size: 3114 2626 688 425 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4566 4812 669 Size: 988 281 2628 246 3079 Fragments arranged by size: 3079 2628 988 281 246 HindIII A'AGCT_T Cuts at: 748 2537 3163 4088 748 Size: 1789 626 925 3882 Fragments arranged by size: 3882 1789 925 626 HpaI GTT'AAC Cuts at: 4812 4812 Size: 7222 KpnI G_GTAC'C Cuts at: 4547 4547 Size: 7222 MfeI C'AATT_G Cuts at: 4821 4821 Size: 7222 MluI A'CGCG_T Cuts at: 7098 7098 Size: 7222 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2097 2163 2337 6336 6520 1305 Size: 792 66 174 3999 184 2007 Fragments arranged by size: 3999 2007 792 184 174 66 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5400 Fragments arranged by size: 5400 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3948 4132 4384 4443 5180 5539 5698 Size: 648 2782 184 252 59 737 359 159 Cuts at: 5698 518 Size: 2042 Fragments arranged by size: 2782 2042 737 648 359 252 184 159 59 MspA1I CmG'CkG Cuts at: 1606 4638 5315 6463 6708 1606 Size: 3032 677 1148 245 2120 Fragments arranged by size: 3032 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5731 Fragments arranged by size: 5731 939 552 NdeI CA'TA_TG Cuts at: 387 4129 4381 387 Size: 3742 252 3228 Fragments arranged by size: 3742 3228 252 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7222 NotI GC'GGCC_GC Cuts at: 4669 4669 Size: 7222 NspI r_CATG'y Cuts at: 1132 1229 2076 4580 6125 1132 Size: 97 847 2504 1545 2229 Fragments arranged by size: 2504 2229 1545 847 97 PasI CC'CwG_GG Cuts at: 1348 2017 2143 1348 Size: 669 126 6427 Fragments arranged by size: 6427 669 126 PciI A'CATG_T Cuts at: 6121 6121 Size: 7222 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7222 PmeI GTTT'AAAC Cuts at: 4526 4526 Size: 7222 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2123 2155 5291 5323 6804 6836 2123 Size: 32 3136 32 1481 32 2509 Fragments arranged by size: 3136 2509 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7222 PsiI TTA'TAA Cuts at: 2688 3039 3459 3795 4792 2688 Size: 351 420 336 997 5118 Fragments arranged by size: 5118 997 420 351 336 PstI C_TGCA'G Cuts at: 830 2592 4574 830 Size: 1762 1982 3478 Fragments arranged by size: 3478 1982 1762 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7222 SacI G_AGCT'C Cuts at: 3157 3157 Size: 7222 SalI G'TCGA_C Cuts at: 1936 4564 1936 Size: 2628 4594 SbfI CC_TGCA'GG Cuts at: 4574 4574 Size: 7222 ScaI AGT'ACT Cuts at: 5470 7182 5470 Size: 1712 5510 SfcI C'TryA_G Cuts at: 826 1045 2046 2588 4570 5705 6386 6577 826 Size: 219 1001 542 1982 1135 681 191 1471 Fragments arranged by size: 1982 1471 1135 1001 681 542 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7222 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7222 SmaI CCC'GGG Cuts at: 1872 4549 1872 Size: 2677 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2426 5326 6227 6489 820 Size: 197 926 483 2900 901 262 1553 Fragments arranged by size: 2900 1553 926 901 483 262 197 SnaBI TAC'GTA Cuts at: 493 5013 493 Size: 4520 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7222 SphI G_CATG'C Cuts at: 1132 4580 1132 Size: 3448 3774 SspI AAT'ATT Cuts at: 5 52 5146 5 Size: 47 5094 2081 Fragments arranged by size: 5094 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7222 StyI C'CwwG_G Cuts at: 513 1065 2004 4661 513 Size: 552 939 2657 3074 Fragments arranged by size: 3074 2657 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2030 5249 5266 5419 5604 6049 325 Size: 1255 450 3219 17 153 185 445 1498 Fragments arranged by size: 3219 1498 1255 450 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2100 2221 5468 Size: 274 80 33 51 1443 123 121 3247 Cuts at: 5468 7180 96 Size: 1712 138 Fragments arranged by size: 3247 1712 1443 274 138 123 121 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7182 7214 7182 Size: 32 7190 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7222 XbaI T'CTAG_A Cuts at: 4558 4558 Size: 7222 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7222 XmaI C'CCGG_G Cuts at: 1870 4547 1870 Size: 2677 4545 XmnI GAAnn'nnTTC Cuts at: 2642 2905 4238 5351 2642 Size: 263 1333 1113 4513 Fragments arranged by size: 4513 1333 1113 263 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5030 276 Size: 53 83 186 936 3496 2468 Fragments arranged by size: 3496 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI