(Circular) (Six-base) MAPSORT of: pFN21AB9758.seq Check: 8626 from: 1 to: 6838 pFN21AB9758 6838 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2846 3068 3187 2846 Size: 222 119 6497 Fragments arranged by size: 6497 222 119 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4648 278 Size: 53 83 186 936 3112 2468 Fragments arranged by size: 3112 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2235 2286 4181 1937 Size: 298 51 1895 4594 Fragments arranged by size: 4594 1895 298 51 Acc65I G'GTAC_C Cuts at: 4159 4159 Size: 6838 AclI AA'CG_TT Cuts at: 1563 4965 5338 1563 Size: 3402 373 3063 Fragments arranged by size: 3402 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2568 3023 3113 4901 6285 6553 1632 Size: 936 455 90 1788 1384 268 1917 Fragments arranged by size: 1917 1788 1384 936 455 268 90 AfeI AGC'GCT Cuts at: 3267 3267 Size: 6838 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6641 AflIII A'CryG_T Cuts at: 1203 3488 5737 6714 1203 Size: 2285 2249 977 1327 Fragments arranged by size: 2285 2249 1327 977 AgeI A'CCGG_T Cuts at: 5669 5669 Size: 6838 AhdI GACnn_n'nnGTC Cuts at: 5567 5567 Size: 6838 AleI CACnn'nnGTG Cuts at: 1166 2094 2526 3038 3462 4031 1166 Size: 928 432 512 424 569 3973 Fragments arranged by size: 3973 928 569 512 432 424 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2769 1184 Size: 1585 5253 AlwNI CAG_nnn'CTG Cuts at: 2861 4581 6153 2861 Size: 1720 1572 3546 Fragments arranged by size: 3546 1720 1572 ApaLI G'TGCA_C Cuts at: 3113 4895 6051 3113 Size: 1782 1156 3900 Fragments arranged by size: 3900 1782 1156 ApoI r'AATT_y Cuts at: 1493 2428 3369 3598 4147 4373 4525 6634 1493 Size: 935 941 229 549 226 152 2109 1697 Fragments arranged by size: 2109 1697 941 935 549 229 226 152 AseI AT'TA_AT Cuts at: 160 1032 5392 160 Size: 872 4360 1606 Fragments arranged by size: 4360 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6838 AvaI C'yCGr_G Cuts at: 1870 1943 4163 1870 Size: 73 2220 4545 Fragments arranged by size: 4545 2220 73 BamHI G'GATC_C Cuts at: 3548 4168 4535 3548 Size: 620 367 5851 Fragments arranged by size: 5851 620 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3222 3326 4159 5614 618 Size: 325 217 627 1435 104 833 1455 1842 Fragments arranged by size: 1842 1455 1435 833 627 325 217 104 BanII G_rGCy'C Cuts at: 1391 4157 1391 Size: 2766 4072 BbeI G_GCGC'C Cuts at: 3330 3330 Size: 6838 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2574 2737 928 Size: 971 675 163 5029 Fragments arranged by size: 5029 971 675 163 BbvCI CC'TCA_GC Cuts at: 2757 2757 Size: 6838 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2057 2091 5029 5063 1903 Size: 34 120 34 2938 34 3678 Fragments arranged by size: 3678 2938 120 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4730 5940 4730 Size: 1210 5628 BclI T'GATC_A Cuts at: 1548 1548 Size: 6838 BfrBI ATG'CAT Cuts at: 2068 2068 Size: 6838 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2153 2495 3240 Size: 107 122 71 631 605 481 342 745 Cuts at: 3240 4016 5449 136 Size: 776 1433 1525 Fragments arranged by size: 1525 1433 776 745 631 605 481 342 122 107 71 BglII A'GATC_T Cuts at: 6833 6833 Size: 6838 BlpI GC'TnA_GC Cuts at: 4073 4255 4073 Size: 182 6656 Bme1580I G_kGCm'C Cuts at: 1790 2816 3117 4899 6055 1790 Size: 1026 301 1782 1156 2573 Fragments arranged by size: 2573 1782 1156 1026 301 BmgBI CAC'GTC Cuts at: 1318 2101 3178 1318 Size: 783 1077 4978 Fragments arranged by size: 4978 1077 783 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2520 4569 5527 Size: 416 53 83 399 24 1103 2049 958 Cuts at: 5527 442 Size: 1753 Fragments arranged by size: 2049 1753 1103 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6838 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3674 5498 1239 Size: 2435 1824 2579 Fragments arranged by size: 2579 2435 1824 Bpu10I CC'TnA_GC Cuts at: 2757 2757 Size: 6838 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2297 3338 4963 5828 6126 2297 Size: 1041 1625 865 298 3009 Fragments arranged by size: 3009 1625 1041 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2537 3291 5501 882 Size: 617 1038 754 2210 2219 Fragments arranged by size: 2219 2210 1038 754 617 BsaAI yAC'GTr Cuts at: 493 1204 3112 3190 4629 493 Size: 711 1908 78 1439 2702 Fragments arranged by size: 2702 1908 1439 711 78 BsaBI GATnn'nnATC Cuts at: 6832 6832 Size: 6838 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3327 4645 5027 5662 Size: 53 83 186 936 1794 1318 382 635 Cuts at: 5662 275 Size: 1451 Fragments arranged by size: 1794 1451 1318 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5271 5669 5943 6090 6501 5271 Size: 398 274 147 411 5608 Fragments arranged by size: 5608 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2656 2686 2861 2891 1178 Size: 30 165 30 1253 30 175 30 5125 Fragments arranged by size: 5125 1253 175 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2912 2921 2924 2927 2930 2933 1186 Size: 1726 9 3 3 3 3 5091 Fragments arranged by size: 5091 1726 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 2684 3591 3675 6041 6537 2684 Size: 907 84 2366 496 2985 Fragments arranged by size: 2985 2366 907 496 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2037 2270 2342 2980 3260 3298 1163 Size: 573 301 233 72 638 280 38 4703 Fragments arranged by size: 4703 638 573 301 280 233 72 38 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4288 5049 6077 1540 Size: 275 186 2287 761 1028 2301 Fragments arranged by size: 2301 2287 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3117 4899 4984 6055 3117 Size: 1782 85 1071 3900 Fragments arranged by size: 3900 1782 1071 85 BsmI GAATG_Cn' Cuts at: 2334 4349 4442 2334 Size: 2015 93 4730 Fragments arranged by size: 4730 2015 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2816 3117 4157 4899 4984 6055 1391 Size: 399 1026 301 1040 742 85 1071 2174 Fragments arranged by size: 2174 1071 1040 1026 742 399 301 85 BspEI T'CCGG_A Cuts at: 6501 6501 Size: 6838 BspHI T'CATG_A Cuts at: 4727 6457 4727 Size: 1730 5108 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2242 2818 2846 3068 3187 4193 844 Size: 1398 576 28 222 119 1006 3489 Fragments arranged by size: 3489 1398 1006 576 222 119 28 BsrBI CCG'CTC Cuts at: 1394 1823 3220 4725 1394 Size: 429 1397 1505 3507 Fragments arranged by size: 3507 1505 1397 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2578 3742 5333 5507 65 Size: 1183 1330 1164 1591 174 1396 Fragments arranged by size: 1591 1396 1330 1183 1164 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3300 5482 5669 1700 Size: 110 1490 2182 187 2869 Fragments arranged by size: 2869 2182 1490 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6838 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6838 BssSI C'ACGA_G Cuts at: 3278 4898 5910 3278 Size: 1620 1012 4206 Fragments arranged by size: 4206 1620 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2065 2329 2967 3193 3321 3741 794 Size: 1271 264 638 226 128 420 3891 Fragments arranged by size: 3891 1271 638 420 264 226 128 BstBI TT'CG_AA Cuts at: 5642 5642 Size: 6838 BstEII G'GTnAC_C Cuts at: 1184 2087 1184 Size: 903 5935 BstXI CCAn_nnnn'nTGG Cuts at: 3040 3040 Size: 6838 BstYI r'GATC_y Cuts at: 1972 2706 3548 4168 4535 4920 4937 6389 6475 Size: 734 842 620 367 385 17 1452 86 Cuts at: 6475 6487 6833 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 842 734 620 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3022 3809 4029 513 Size: 552 939 1018 787 220 3322 Fragments arranged by size: 3322 1018 939 787 552 220 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6099 Fragments arranged by size: 6099 683 56 BtsI GCAGTG_nn' Cuts at: 2163 2289 2467 4354 5148 5168 2163 Size: 126 178 1887 794 20 3833 Fragments arranged by size: 3833 1887 794 178 126 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6838 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6803 DraI TTT'AAA Cuts at: 3562 4142 3562 Size: 580 6258 DraIII CAC_nnn'GTG Cuts at: 3028 3321 3028 Size: 293 6545 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2105 5845 809 Size: 507 789 3740 1802 Fragments arranged by size: 3740 1802 789 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2505 3030 3064 Size: 54 1425 50 275 18 675 525 34 Cuts at: 3064 3232 3937 4053 4285 5174 6803 8 Size: 168 705 116 232 889 1629 43 Fragments arranged by size: 1629 1425 889 705 675 525 275 232 168 116 54 50 43 34 18 EagI C'GGCC_G Cuts at: 1537 1812 4285 1537 Size: 275 2473 4090 Fragments arranged by size: 4090 2473 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2201 2898 2939 2987 3089 3777 3879 4768 Size: 848 697 41 48 102 688 102 889 Cuts at: 4768 1353 Size: 3423 Fragments arranged by size: 3423 889 848 697 688 102 102 48 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5425 5799 5945 387 Size: 1064 138 3836 374 146 1280 Fragments arranged by size: 3836 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2568 3023 3113 3674 4901 5498 6285 Size: 393 936 455 90 561 1227 597 787 Cuts at: 6285 6553 1239 Size: 268 1524 Fragments arranged by size: 1524 1227 936 787 597 561 455 393 268 90 EcoO109I rG'GnC_Cy Cuts at: 3247 3247 Size: 6838 EcoRI G'AATT_C Cuts at: 3369 3598 4147 4525 3369 Size: 229 549 378 5682 Fragments arranged by size: 5682 549 378 229 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2213 2968 3535 2213 Size: 755 567 5516 Fragments arranged by size: 5516 755 567 FspI TGC'GCA Cuts at: 5344 5344 Size: 6838 HaeII r_GCGC'y Cuts at: 1678 3109 3130 3269 3330 3697 5985 1678 Size: 1431 21 139 61 367 2288 2531 Fragments arranged by size: 2531 2288 1431 367 139 61 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2861 2893 3878 3910 5479 Size: 32 135 32 1289 32 985 32 1569 Cuts at: 5479 5511 5553 5585 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1569 1289 985 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4182 4428 669 Size: 988 281 2244 246 3079 Fragments arranged by size: 3079 2244 988 281 246 HindIII A'AGCT_T Cuts at: 748 3902 748 Size: 3154 3684 HpaI GTT'AAC Cuts at: 4428 4428 Size: 6838 KasI G'GCGC_C Cuts at: 3326 3326 Size: 6838 KpnI G_GTAC'C Cuts at: 4163 4163 Size: 6838 MfeI C'AATT_G Cuts at: 4437 4437 Size: 6838 MluI A'CGCG_T Cuts at: 6714 6714 Size: 6838 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2847 5952 6136 1305 Size: 1542 3105 184 2007 Fragments arranged by size: 3105 2007 1542 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2507 3032 3939 4055 10 Size: 54 1425 343 675 525 907 116 2793 Fragments arranged by size: 2793 1425 907 675 525 343 116 54 MslI CAynn'nnrTG Cuts at: 518 1166 2094 2526 2819 3038 3462 3730 4031 Size: 648 928 432 293 219 424 268 301 Cuts at: 4031 4796 5155 5314 518 Size: 765 359 159 2042 Fragments arranged by size: 2042 928 765 648 432 424 359 301 293 268 219 159 MspA1I CmG'CkG Cuts at: 1606 3051 3084 3228 3595 4254 4931 6079 6324 Size: 1445 33 144 367 659 677 1148 245 Cuts at: 6324 1606 Size: 2120 Fragments arranged by size: 2120 1445 1148 677 659 367 245 144 33 NarI GG'CG_CC Cuts at: 3327 3327 Size: 6838 NcoI C'CATG_G Cuts at: 513 1065 2004 3809 513 Size: 552 939 1805 3542 Fragments arranged by size: 3542 1805 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6838 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6838 NotI GC'GGCC_GC Cuts at: 4285 4285 Size: 6838 NsiI A_TGCA'T Cuts at: 2070 2070 Size: 6838 NspI r_CATG'y Cuts at: 1132 1229 2581 3436 3492 4196 5741 1132 Size: 97 1352 855 56 704 1545 2229 Fragments arranged by size: 2229 1545 1352 855 704 97 56 PasI CC'CwG_GG Cuts at: 1348 2493 1348 Size: 1145 5693 PciI A'CATG_T Cuts at: 3488 5737 3488 Size: 2249 4589 PflMI CCAn_nnn'nTGG Cuts at: 1108 3028 3952 1108 Size: 1920 924 3994 Fragments arranged by size: 3994 1920 924 PmeI GTTT'AAAC Cuts at: 4142 4142 Size: 6838 PmlI CAC'GTG Cuts at: 3112 3190 3112 Size: 78 6760 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4907 4939 6420 6452 4907 Size: 32 1481 32 5293 Fragments arranged by size: 5293 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2231 1613 Size: 618 6220 PsiI TTA'TAA Cuts at: 4408 4408 Size: 6838 PstI C_TGCA'G Cuts at: 830 3198 3961 4190 830 Size: 2368 763 229 3478 Fragments arranged by size: 3478 2368 763 229 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6838 SalI G'TCGA_C Cuts at: 1936 4180 1936 Size: 2244 4594 SapI GCTCTTCn'nnn_ Cuts at: 2939 2987 3089 2939 Size: 48 102 6688 Fragments arranged by size: 6688 102 48 SbfI CC_TGCA'GG Cuts at: 4190 4190 Size: 6838 ScaI AGT'ACT Cuts at: 2412 3518 5086 6798 2412 Size: 1106 1568 1712 2452 Fragments arranged by size: 2452 1712 1568 1106 SfcI C'TryA_G Cuts at: 826 1045 2776 3194 3957 4186 5321 6002 6193 Size: 219 1731 418 763 229 1135 681 191 Cuts at: 6193 826 Size: 1471 Fragments arranged by size: 1731 1471 1135 763 681 418 229 219 191 SfoI GGC'GCC Cuts at: 3328 3328 Size: 6838 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6838 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6838 SmaI CCC'GGG Cuts at: 1872 4165 1872 Size: 2293 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2276 3353 4942 5843 6105 820 Size: 197 926 333 1077 1589 901 262 1553 Fragments arranged by size: 1589 1553 1077 926 901 333 262 197 SnaBI TAC'GTA Cuts at: 493 4629 493 Size: 4136 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6838 SphI G_CATG'C Cuts at: 1132 4196 1132 Size: 3064 3774 SspI AAT'ATT Cuts at: 5 52 4762 5 Size: 47 4710 2081 Fragments arranged by size: 4710 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6838 StyI C'CwwG_G Cuts at: 513 1065 2004 2544 2607 3809 4277 513 Size: 552 939 540 63 1202 468 3074 Fragments arranged by size: 3074 1202 939 552 540 468 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3689 4865 4882 5035 5220 5665 325 Size: 1255 2109 1176 17 153 185 445 1498 Fragments arranged by size: 2109 1498 1255 1176 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2410 3516 5084 Size: 274 80 33 51 1443 433 1106 1568 Cuts at: 5084 6796 96 Size: 1712 138 Fragments arranged by size: 1712 1568 1443 1106 433 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2656 2688 2765 2797 6798 6830 2656 Size: 32 77 32 4001 32 2664 Fragments arranged by size: 4001 2664 77 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6838 XbaI T'CTAG_A Cuts at: 4174 4174 Size: 6838 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2500 2500 Size: 6838 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6838 XmaI C'CCGG_G Cuts at: 1870 4163 1870 Size: 2293 4545 XmnI GAAnn'nnTTC Cuts at: 2996 3158 3818 4967 2996 Size: 162 660 1149 4867 Fragments arranged by size: 4867 1149 660 162 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4646 276 Size: 53 83 186 936 3112 2468 Fragments arranged by size: 3112 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI