(Circular) (Six-base) MAPSORT of: pFN21AB9756.seq Check: 4689 from: 1 to: 6820 pFN21AB9756 6820 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4630 278 Size: 53 83 186 936 3094 2468 Fragments arranged by size: 3094 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2811 3966 4163 1937 Size: 874 1155 197 4594 Fragments arranged by size: 4594 1155 874 197 Acc65I G'GTAC_C Cuts at: 4141 4141 Size: 6820 AclI AA'CG_TT Cuts at: 1563 4947 5320 1563 Size: 3384 373 3063 Fragments arranged by size: 3384 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3349 3381 3710 3885 4077 4883 6267 6535 Size: 1717 32 329 175 192 806 1384 268 Cuts at: 6535 1632 Size: 1917 Fragments arranged by size: 1917 1717 1384 806 329 268 192 175 32 AfeI AGC'GCT Cuts at: 2101 2278 2101 Size: 177 6643 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6623 AflIII A'CryG_T Cuts at: 1203 5719 6696 1203 Size: 4516 977 1327 Fragments arranged by size: 4516 1327 977 AgeI A'CCGG_T Cuts at: 5651 5651 Size: 6820 AhdI GACnn_n'nnGTC Cuts at: 2155 5549 2155 Size: 3394 3426 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6820 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3309 1184 Size: 2125 4695 AlwNI CAG_nnn'CTG Cuts at: 2792 3302 4563 6135 2792 Size: 510 1261 1572 3477 Fragments arranged by size: 3477 1572 1261 510 ApaI G_GGCC'C Cuts at: 2230 2304 2230 Size: 74 6746 ApaLI G'TGCA_C Cuts at: 4877 6033 4877 Size: 1156 5664 ApoI r'AATT_y Cuts at: 1493 2840 4021 4129 4355 4507 6616 1493 Size: 1347 1181 108 226 152 2109 1697 Fragments arranged by size: 2109 1697 1347 1181 226 152 108 AseI AT'TA_AT Cuts at: 160 1032 4050 5374 160 Size: 872 3018 1324 1606 Fragments arranged by size: 3018 1606 1324 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6820 AvaI C'yCGr_G Cuts at: 1870 1943 2612 2686 4145 1870 Size: 73 669 74 1459 4545 Fragments arranged by size: 4545 1459 669 74 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2516 2549 2516 Size: 33 6787 BamHI G'GATC_C Cuts at: 4150 4517 4150 Size: 367 6453 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2515 2690 4141 5596 618 Size: 325 217 627 728 175 1451 1455 1842 Fragments arranged by size: 1842 1455 1451 728 627 325 217 175 BanII G_rGCy'C Cuts at: 1391 2036 2208 2230 2304 2324 2426 2716 3252 Size: 645 172 22 74 20 102 290 536 Cuts at: 3252 3498 4139 1391 Size: 246 641 4072 Fragments arranged by size: 4072 645 641 536 290 246 172 102 74 22 20 BbeI G_GCGC'C Cuts at: 2519 2519 Size: 6820 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5849 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5011 5045 1903 Size: 34 50 34 2990 34 3678 Fragments arranged by size: 3678 2990 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4712 5922 4712 Size: 1210 5610 BclI T'GATC_A Cuts at: 1548 2865 3340 1548 Size: 1317 475 5028 Fragments arranged by size: 5028 1317 475 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2234 2319 2384 Size: 107 122 71 631 605 562 85 65 Cuts at: 2384 2747 2833 5431 136 Size: 363 86 2598 1525 Fragments arranged by size: 2598 1525 631 605 562 363 122 107 86 85 71 65 BglII A'GATC_T Cuts at: 6815 6815 Size: 6820 BlpI GC'TnA_GC Cuts at: 4237 4237 Size: 6820 Bme1580I G_kGCm'C Cuts at: 1790 2230 2304 2609 2693 2806 4881 6037 1790 Size: 440 74 305 84 113 2075 1156 2573 Fragments arranged by size: 2573 2075 1156 440 305 113 84 74 BmgBI CAC'GTC Cuts at: 1318 2809 1318 Size: 1491 5329 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2777 4551 5509 Size: 416 53 83 399 24 1360 1774 958 Cuts at: 5509 442 Size: 1753 Fragments arranged by size: 1774 1753 1360 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6820 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2365 2365 Size: 6820 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2090 2426 2757 3480 3823 3994 5480 1239 Size: 851 336 331 723 343 171 1486 2579 Fragments arranged by size: 2579 1486 851 723 343 336 331 171 Bpu10I CC'TnA_GC Cuts at: 2420 2420 Size: 6820 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2923 3501 4945 5810 6108 2923 Size: 578 1444 865 298 3635 Fragments arranged by size: 3635 1444 865 578 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5483 882 Size: 617 3984 2219 Fragments arranged by size: 3984 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4611 493 Size: 711 3407 2702 Fragments arranged by size: 3407 2702 711 BsaBI GATnn'nnATC Cuts at: 6814 6814 Size: 6820 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2256 2516 4627 5009 Size: 53 83 186 936 723 260 2111 382 Cuts at: 5009 5644 275 Size: 635 1451 Fragments arranged by size: 2111 1451 936 723 635 382 260 186 83 53 BsaWI w'CCGG_w Cuts at: 5253 5651 5925 6072 6483 5253 Size: 398 274 147 411 5590 Fragments arranged by size: 5590 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3406 3418 3436 3448 1178 Size: 30 165 30 2003 12 18 12 4550 Fragments arranged by size: 4550 2003 165 30 30 18 12 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2121 2351 2354 1186 Size: 935 230 3 5652 Fragments arranged by size: 5652 935 230 3 BseYI C'CCAG_C Cuts at: 2114 2510 3606 6023 6519 2114 Size: 396 1096 2417 496 2415 Fragments arranged by size: 2417 2415 1096 496 396 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6247 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2314 4270 5031 6059 1540 Size: 275 186 313 1956 761 1028 2301 Fragments arranged by size: 2301 1956 1028 761 313 275 186 BsiHKAI G_wGCw'C Cuts at: 2036 2426 4881 4966 6037 2036 Size: 390 2455 85 1071 2819 Fragments arranged by size: 2819 2455 1071 390 85 BsmI GAATG_Cn' Cuts at: 4331 4424 4331 Size: 93 6727 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2208 2230 2304 2324 2426 2609 Size: 399 246 172 22 74 20 102 183 Cuts at: 2609 2693 2716 2806 3252 3498 4139 4881 4966 Size: 84 23 90 446 246 641 742 85 Cuts at: 4966 6037 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 641 446 399 246 246 183 172 102 90 85 84 74 23 22 20 BspEI T'CCGG_A Cuts at: 6483 6483 Size: 6820 BspHI T'CATG_A Cuts at: 4709 6439 4709 Size: 1730 5090 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2052 4175 844 Size: 1208 2123 3489 Fragments arranged by size: 3489 2123 1208 BsrBI CCG'CTC Cuts at: 1394 1823 2199 2431 2719 4707 1394 Size: 429 376 232 288 1988 3507 Fragments arranged by size: 3507 1988 429 376 288 232 BsrDI GCAATG_nn' Cuts at: 65 1248 3291 3692 3780 5315 5489 65 Size: 1183 2043 401 88 1535 174 1396 Fragments arranged by size: 2043 1535 1396 1183 401 174 88 BsrFI r'CCGG_y Cuts at: 1700 1810 2639 2823 5464 5651 1700 Size: 110 829 184 2641 187 2869 Fragments arranged by size: 2869 2641 829 187 184 110 BsrGI T'GTAC_A Cuts at: 96 3387 96 Size: 3291 3529 BssHII G'CGCG_C Cuts at: 1925 2052 2399 2552 1925 Size: 127 347 153 6193 Fragments arranged by size: 6193 347 153 127 BssSI C'ACGA_G Cuts at: 4880 5892 4880 Size: 1012 5808 BstAPI GCAn_nnn'nTGC Cuts at: 794 3302 794 Size: 2508 4312 BstBI TT'CG_AA Cuts at: 5624 5624 Size: 6820 BstEII G'GTnAC_C Cuts at: 1184 3007 1184 Size: 1823 4997 BstXI CCAn_nnnn'nTGG Cuts at: 3638 3638 Size: 6820 BstYI r'GATC_y Cuts at: 1972 2753 3440 4150 4517 4902 4919 6371 6457 Size: 781 687 710 367 385 17 1452 86 Cuts at: 6457 6469 6815 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 781 710 687 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3967 3967 Size: 6820 Bsu36I CC'TnA_GG Cuts at: 2457 2457 Size: 6820 BtgI C'CryG_G Cuts at: 513 1065 2232 2382 2409 2521 2595 3426 3830 Size: 552 1167 150 27 112 74 831 404 Cuts at: 3830 513 Size: 3503 Fragments arranged by size: 3503 1167 831 552 404 150 112 74 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6081 Fragments arranged by size: 6081 683 56 BtsI GCAGTG_nn' Cuts at: 2292 2394 2656 2931 3303 4336 5130 5150 2292 Size: 102 262 275 372 1033 794 20 3962 Fragments arranged by size: 3962 1033 794 372 275 262 102 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6820 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6785 DraI TTT'AAA Cuts at: 4124 4124 Size: 6820 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5827 809 Size: 507 4511 1802 Fragments arranged by size: 4511 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2104 2235 2289 Size: 54 1425 50 275 18 274 131 54 Cuts at: 2289 2311 2391 3594 4267 5156 6785 8 Size: 22 80 1203 673 889 1629 43 Fragments arranged by size: 1629 1425 1203 889 673 275 274 131 80 54 54 50 43 22 18 EagI C'GGCC_G Cuts at: 1537 1812 2311 4267 1537 Size: 275 499 1956 4090 Fragments arranged by size: 4090 1956 499 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2495 3582 3913 4053 4750 1353 Size: 1142 1087 331 140 697 3423 Fragments arranged by size: 3423 1142 1087 697 331 140 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2341 2984 5407 5781 5927 387 Size: 1064 138 752 643 2423 374 146 1280 Fragments arranged by size: 2423 1280 1064 752 643 374 146 138 EcoICRI GAG'CTC Cuts at: 2034 2424 2034 Size: 390 6430 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2426 2757 3349 3381 3480 3710 Size: 393 458 336 331 592 32 99 230 Cuts at: 3710 3823 3885 3994 4077 4883 5480 6267 6535 Size: 113 62 109 83 806 597 787 268 Cuts at: 6535 1239 Size: 1524 Fragments arranged by size: 1524 806 787 597 592 458 393 336 331 268 230 113 109 99 83 62 32 EcoO109I rG'GnC_Cy Cuts at: 2226 2227 2300 2301 2773 2226 Size: 1 73 1 472 6273 Fragments arranged by size: 6273 472 73 1 1 EcoRI G'AATT_C Cuts at: 4129 4507 4129 Size: 378 6442 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2161 2161 Size: 6820 FspI TGC'GCA Cuts at: 5326 5326 Size: 6820 HaeII r_GCGC'y Cuts at: 1678 2103 2280 2519 2989 5967 1678 Size: 425 177 239 470 2978 2531 Fragments arranged by size: 2978 2531 470 425 239 177 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2141 2173 3860 3892 5461 Size: 32 135 32 569 32 1687 32 1569 Cuts at: 5461 5493 5535 5567 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1687 1569 569 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3414 3505 4164 4410 669 Size: 988 281 1476 91 659 246 3079 Fragments arranged by size: 3079 1476 988 659 281 246 91 HindIII A'AGCT_T Cuts at: 748 748 Size: 6820 HpaI GTT'AAC Cuts at: 3414 4410 3414 Size: 996 5824 KasI G'GCGC_C Cuts at: 2515 2515 Size: 6820 KpnI G_GTAC'C Cuts at: 4145 4145 Size: 6820 MfeI C'AATT_G Cuts at: 4419 4419 Size: 6820 MluI A'CGCG_T Cuts at: 6696 6696 Size: 6820 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2121 2445 5934 6118 1305 Size: 816 324 3489 184 2007 Fragments arranged by size: 3489 2007 816 324 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2106 2291 3596 10 Size: 54 1425 343 274 185 1305 3234 Fragments arranged by size: 3234 1425 1305 343 274 185 54 MslI CAynn'nnrTG Cuts at: 518 1166 2943 3161 3296 4778 5137 5296 518 Size: 648 1777 218 135 1482 359 159 2042 Fragments arranged by size: 2042 1777 1482 648 359 218 159 135 MspA1I CmG'CkG Cuts at: 1606 2068 2091 2234 2357 2384 2597 2670 2747 Size: 462 23 143 123 27 213 73 77 Cuts at: 2747 3610 3821 4236 4913 6061 6306 1606 Size: 863 211 415 677 1148 245 2120 Fragments arranged by size: 2120 1148 863 677 462 415 245 213 211 143 123 77 73 27 23 NarI GG'CG_CC Cuts at: 2516 2516 Size: 6820 NcoI C'CATG_G Cuts at: 513 1065 2409 3830 513 Size: 552 1344 1421 3503 Fragments arranged by size: 3503 1421 1344 552 NdeI CA'TA_TG Cuts at: 387 3703 387 Size: 3316 3504 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6820 NotI GC'GGCC_GC Cuts at: 4267 4267 Size: 6820 NspI r_CATG'y Cuts at: 1132 1229 2893 3037 4178 5723 1132 Size: 97 1664 144 1141 1545 2229 Fragments arranged by size: 2229 1664 1545 1141 144 97 PasI CC'CwG_GG Cuts at: 1348 3742 1348 Size: 2394 4426 PciI A'CATG_T Cuts at: 5719 5719 Size: 6820 PflMI CCAn_nnn'nTGG Cuts at: 1108 2792 2982 3742 1108 Size: 1684 190 760 4186 Fragments arranged by size: 4186 1684 760 190 PfoI T'CCnGG_A Cuts at: 3489 3489 Size: 6820 PmeI GTTT'AAAC Cuts at: 4124 4124 Size: 6820 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4889 4921 6402 6434 4889 Size: 32 1481 32 5275 Fragments arranged by size: 5275 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6820 PsiI TTA'TAA Cuts at: 3531 4390 3531 Size: 859 5961 PspOMI G'GGCC_C Cuts at: 2226 2300 2226 Size: 74 6746 PstI C_TGCA'G Cuts at: 830 2362 4172 830 Size: 1532 1810 3478 Fragments arranged by size: 3478 1810 1532 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6820 PvuII CAG'CTG Cuts at: 3821 3821 Size: 6820 SacI G_AGCT'C Cuts at: 2036 2426 2036 Size: 390 6430 SacII CC_GC'GG Cuts at: 2235 2385 2598 2235 Size: 150 213 6457 Fragments arranged by size: 6457 213 150 SalI G'TCGA_C Cuts at: 1936 4162 1936 Size: 2226 4594 SapI GCTCTTCn'nnn_ Cuts at: 3913 3913 Size: 6820 SbfI CC_TGCA'GG Cuts at: 4172 4172 Size: 6820 ScaI AGT'ACT Cuts at: 5068 6780 5068 Size: 1712 5108 SfcI C'TryA_G Cuts at: 826 1045 2358 4168 5303 5984 6175 826 Size: 219 1313 1810 1135 681 191 1471 Fragments arranged by size: 1810 1471 1313 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2234 2234 Size: 6820 SfoI GGC'GCC Cuts at: 2517 2517 Size: 6820 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6820 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6820 SmaI CCC'GGG Cuts at: 1872 2614 2688 4147 1872 Size: 742 74 1459 4545 Fragments arranged by size: 4545 1459 742 74 SmlI C'TyrA_G Cuts at: 820 1017 1943 2902 3516 4924 5825 6087 820 Size: 197 926 959 614 1408 901 262 1553 Fragments arranged by size: 1553 1408 959 926 901 614 262 197 SnaBI TAC'GTA Cuts at: 493 4611 493 Size: 4118 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6820 SphI G_CATG'C Cuts at: 1132 4178 1132 Size: 3046 3774 SrfI GCCC'GGGC Cuts at: 2688 2688 Size: 6820 SspI AAT'ATT Cuts at: 5 52 4744 5 Size: 47 4692 2081 Fragments arranged by size: 4692 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6820 StyI C'CwwG_G Cuts at: 513 1065 2409 3830 3879 4259 513 Size: 552 1344 1421 49 380 3074 Fragments arranged by size: 3074 1421 1344 552 380 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2995 3075 3173 4847 4864 5017 5202 Size: 1255 1415 80 98 1674 17 153 185 Cuts at: 5202 5647 325 Size: 445 1498 Fragments arranged by size: 1674 1498 1415 1255 445 185 153 98 80 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2700 3387 5066 Size: 274 80 33 51 1443 723 687 1679 Cuts at: 5066 6778 96 Size: 1712 138 Fragments arranged by size: 1712 1679 1443 723 687 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3418 3450 6780 6812 3418 Size: 32 3330 32 3426 Fragments arranged by size: 3426 3330 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6820 XbaI T'CTAG_A Cuts at: 3943 4156 3943 Size: 213 6607 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2507 2507 Size: 6820 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6820 XmaI C'CCGG_G Cuts at: 1870 2612 2686 4145 1870 Size: 742 74 1459 4545 Fragments arranged by size: 4545 1459 742 74 XmnI GAAnn'nnTTC Cuts at: 4949 4949 Size: 6820 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4628 276 Size: 53 83 186 936 3094 2468 Fragments arranged by size: 3094 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BbvCI BfrBI BsiWI BsmBI DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SexAI SwaI