(Circular) (Six-base) MAPSORT of: pFN21AB9755.seq Check: 8610 from: 1 to: 6688 pFN21AB9755 6688 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2181 2181 Size: 6688 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4498 278 Size: 53 83 186 936 2962 2468 Fragments arranged by size: 2962 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4031 1937 Size: 2094 4594 Acc65I G'GTAC_C Cuts at: 4009 4009 Size: 6688 AclI AA'CG_TT Cuts at: 1563 4815 5188 1563 Size: 3252 373 3063 Fragments arranged by size: 3252 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2077 4751 6135 6403 1632 Size: 445 2674 1384 268 1917 Fragments arranged by size: 2674 1917 1384 445 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6491 AflIII A'CryG_T Cuts at: 1203 2828 5587 6564 1203 Size: 1625 2759 977 1327 Fragments arranged by size: 2759 1625 1327 977 AgeI A'CCGG_T Cuts at: 5519 5519 Size: 6688 AhdI GACnn_n'nnGTC Cuts at: 5417 5417 Size: 6688 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6688 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6688 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2514 2546 2514 Size: 32 6656 AlwNI CAG_nnn'CTG Cuts at: 2052 2116 3426 3930 4431 6003 2052 Size: 64 1310 504 501 1572 2737 Fragments arranged by size: 2737 1572 1310 504 501 64 ApaLI G'TGCA_C Cuts at: 4745 5901 4745 Size: 1156 5532 ApoI r'AATT_y Cuts at: 1493 2288 2316 2525 2801 2837 2886 3028 3222 Size: 795 28 209 276 36 49 142 194 Cuts at: 3222 3306 3390 3474 3622 3726 3810 3874 3997 Size: 84 84 84 148 104 84 64 123 Cuts at: 3997 4223 4375 6484 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 795 276 226 209 194 152 148 142 123 104 84 84 84 84 64 49 36 28 AseI AT'TA_AT Cuts at: 160 1032 3038 5242 160 Size: 872 2006 2204 1606 Fragments arranged by size: 2204 2006 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6688 AvaI C'yCGr_G Cuts at: 1870 1943 4013 1870 Size: 73 2070 4545 Fragments arranged by size: 4545 2070 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2123 2156 2123 Size: 33 6655 BamHI G'GATC_C Cuts at: 4018 4385 4018 Size: 367 6321 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4009 5464 618 Size: 325 217 627 2222 1455 1842 Fragments arranged by size: 2222 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4007 1391 Size: 2616 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2364 928 Size: 971 465 5252 Fragments arranged by size: 5252 971 465 BbvCI CC'TCA_GC Cuts at: 2768 2768 Size: 6688 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4879 4913 1903 Size: 34 50 34 2858 34 3678 Fragments arranged by size: 3678 2858 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4580 5790 4580 Size: 1210 5478 BclI T'GATC_A Cuts at: 1548 1548 Size: 6688 BfrBI ATG'CAT Cuts at: 3046 3298 3466 3970 3046 Size: 252 168 504 5764 Fragments arranged by size: 5764 504 252 168 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5299 136 Size: 107 122 71 631 605 3627 1525 Fragments arranged by size: 3627 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2847 6683 2847 Size: 3836 2852 BlpI GC'TnA_GC Cuts at: 2915 4105 2915 Size: 1190 5498 Bme1580I G_kGCm'C Cuts at: 1790 4749 5905 1790 Size: 2959 1156 2573 Fragments arranged by size: 2959 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6688 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4419 5377 442 Size: 416 53 83 399 24 3002 958 1753 Fragments arranged by size: 3002 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6688 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3173 3173 Size: 6688 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3168 3252 3420 3443 3588 3672 3924 5348 Size: 1929 84 168 23 145 84 252 1424 Cuts at: 5348 1239 Size: 2579 Fragments arranged by size: 2579 1929 1424 252 168 145 84 84 23 Bpu10I CC'TnA_GC Cuts at: 2768 2768 Size: 6688 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4813 5678 5976 4813 Size: 865 298 5525 Fragments arranged by size: 5525 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5351 882 Size: 617 3852 2219 Fragments arranged by size: 3852 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4479 493 Size: 711 3275 2702 Fragments arranged by size: 3275 2702 711 BsaBI GATnn'nnATC Cuts at: 6682 6682 Size: 6688 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2134 4495 4877 5512 Size: 53 83 186 936 601 2361 382 635 Cuts at: 5512 275 Size: 1451 Fragments arranged by size: 2361 1451 936 635 601 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5121 5519 5793 5940 6351 5121 Size: 398 274 147 411 5458 Fragments arranged by size: 5458 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6463 Fragments arranged by size: 6463 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 2326 2868 1186 Size: 931 209 542 5006 Fragments arranged by size: 5006 931 542 209 BseYI C'CCAG_C Cuts at: 2028 5891 6387 2028 Size: 3863 496 2329 Fragments arranged by size: 3863 2329 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3861 1163 Size: 573 2125 3990 Fragments arranged by size: 3990 2125 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4138 4899 5927 1540 Size: 275 186 2137 761 1028 2301 Fragments arranged by size: 2301 2137 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4749 4834 5905 4749 Size: 85 1071 5532 Fragments arranged by size: 5532 1071 85 BsmI GAATG_Cn' Cuts at: 3170 3422 4199 4292 3170 Size: 252 777 93 5566 Fragments arranged by size: 5566 777 252 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4007 4749 4834 5905 1391 Size: 399 2217 742 85 1071 2174 Fragments arranged by size: 2217 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6351 6351 Size: 6688 BspHI T'CATG_A Cuts at: 2908 4577 6307 2908 Size: 1669 1730 3289 Fragments arranged by size: 3289 1730 1669 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2181 4043 844 Size: 1337 1862 3489 Fragments arranged by size: 3489 1862 1337 BsrBI CCG'CTC Cuts at: 1394 1823 4575 1394 Size: 429 2752 3507 Fragments arranged by size: 3507 2752 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2644 3091 5183 5357 65 Size: 1183 1396 447 2092 174 1396 Fragments arranged by size: 2092 1396 1396 1183 447 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5332 5519 1700 Size: 110 3522 187 2869 Fragments arranged by size: 3522 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6688 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6688 BssSI C'ACGA_G Cuts at: 4748 5760 4748 Size: 1012 5676 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6688 BstBI TT'CG_AA Cuts at: 2190 5492 2190 Size: 3302 3386 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6688 BstYI r'GATC_y Cuts at: 1972 2847 4018 4385 4770 4787 6239 6325 6337 Size: 875 1171 367 385 17 1452 86 12 Cuts at: 6337 6683 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1171 875 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2240 513 Size: 552 1175 4961 Fragments arranged by size: 4961 1175 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3956 487 Size: 683 56 2730 3219 Fragments arranged by size: 3219 2730 683 56 BtsI GCAGTG_nn' Cuts at: 2053 3175 3259 3427 4204 4998 5018 2053 Size: 1122 84 168 777 794 20 3723 Fragments arranged by size: 3723 1122 794 777 168 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6688 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3073 3108 529 Size: 35 2509 35 4109 Fragments arranged by size: 4109 2509 35 35 DraI TTT'AAA Cuts at: 3992 3992 Size: 6688 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5695 809 Size: 507 4379 1802 Fragments arranged by size: 4379 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4135 5024 6653 Size: 54 1425 50 275 18 2305 889 1629 Cuts at: 6653 8 Size: 43 Fragments arranged by size: 2305 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4135 1537 Size: 275 2323 4090 Fragments arranged by size: 4090 2323 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2588 2701 4618 1353 Size: 1235 113 1917 3423 Fragments arranged by size: 3423 1917 1235 113 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5275 5649 5795 387 Size: 1064 138 3686 374 146 1280 Fragments arranged by size: 3686 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2077 3168 3252 3420 3443 3588 3672 Size: 393 445 1091 84 168 23 145 84 Cuts at: 3672 3924 4751 5348 6135 6403 1239 Size: 252 827 597 787 268 1524 Fragments arranged by size: 1524 1091 827 787 597 445 393 268 252 168 145 84 84 23 EcoNI CCTnn'n_nnAGG Cuts at: 2141 2141 Size: 6688 EcoO109I rG'GnC_Cy Cuts at: 3324 3687 3324 Size: 363 6325 EcoRI G'AATT_C Cuts at: 3028 3306 3390 3474 3726 3810 3997 4375 3028 Size: 278 84 84 252 84 187 378 5341 Fragments arranged by size: 5341 378 278 252 187 84 84 84 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3388 3388 Size: 6688 FspI TGC'GCA Cuts at: 5194 5194 Size: 6688 HaeII r_GCGC'y Cuts at: 1678 5835 1678 Size: 4157 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3367 3399 3703 3735 5329 Size: 32 135 32 1795 32 304 32 1594 Cuts at: 5329 5361 5403 5435 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1795 1594 304 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4032 4278 669 Size: 988 281 2094 246 3079 Fragments arranged by size: 3079 2094 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6688 HpaI GTT'AAC Cuts at: 4278 4278 Size: 6688 KpnI G_GTAC'C Cuts at: 4013 4013 Size: 6688 MfeI C'AATT_G Cuts at: 4287 4287 Size: 6688 MluI A'CGCG_T Cuts at: 6564 6564 Size: 6688 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2136 2202 5802 5986 1305 Size: 831 66 3600 184 2007 Fragments arranged by size: 3600 2007 831 184 66 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4866 Fragments arranged by size: 4866 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2046 2577 2979 3835 4646 5005 5164 Size: 648 880 531 402 856 811 359 159 Cuts at: 5164 518 Size: 2042 Fragments arranged by size: 2042 880 856 811 648 531 402 359 159 MspA1I CmG'CkG Cuts at: 1606 4104 4781 5929 6174 1606 Size: 2498 677 1148 245 2120 Fragments arranged by size: 2498 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2240 513 Size: 552 1175 4961 Fragments arranged by size: 4961 1175 552 NdeI CA'TA_TG Cuts at: 387 2996 387 Size: 2609 4079 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6688 NotI GC'GGCC_GC Cuts at: 4135 4135 Size: 6688 NsiI A_TGCA'T Cuts at: 3048 3300 3468 3972 3048 Size: 252 168 504 5764 Fragments arranged by size: 5764 504 252 168 NspI r_CATG'y Cuts at: 1132 1229 2832 4046 5591 1132 Size: 97 1603 1214 1545 2229 Fragments arranged by size: 2229 1603 1545 1214 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6688 PciI A'CATG_T Cuts at: 2828 5587 2828 Size: 2759 3929 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6688 PmeI GTTT'AAAC Cuts at: 3992 3992 Size: 6688 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4757 4789 6270 6302 4757 Size: 32 1481 32 5143 Fragments arranged by size: 5143 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3324 3324 Size: 6688 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6688 PsiI TTA'TAA Cuts at: 3750 4258 3750 Size: 508 6180 PstI C_TGCA'G Cuts at: 830 4040 830 Size: 3210 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6688 SalI G'TCGA_C Cuts at: 1936 4030 1936 Size: 2094 4594 SbfI CC_TGCA'GG Cuts at: 4040 4040 Size: 6688 ScaI AGT'ACT Cuts at: 4936 6648 4936 Size: 1712 4976 SfcI C'TryA_G Cuts at: 826 1045 2164 3735 4036 5171 5852 6043 826 Size: 219 1119 1571 301 1135 681 191 1471 Fragments arranged by size: 1571 1471 1135 1119 681 301 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6688 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6688 SmaI CCC'GGG Cuts at: 1872 4015 1872 Size: 2143 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4792 5693 5955 820 Size: 197 926 2849 901 262 1553 Fragments arranged by size: 2849 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4479 493 Size: 3986 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6688 SphI G_CATG'C Cuts at: 1132 4046 1132 Size: 2914 3774 SspI AAT'ATT Cuts at: 5 52 2484 3979 4612 5 Size: 47 2432 1495 633 2081 Fragments arranged by size: 2432 2081 1495 633 47 StuI AGG'CCT Cuts at: 1517 3017 3353 3605 1517 Size: 1500 336 252 4600 Fragments arranged by size: 4600 1500 336 252 StyI C'CwwG_G Cuts at: 513 1065 2240 4127 513 Size: 552 1175 1887 3074 Fragments arranged by size: 3074 1887 1175 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2870 3962 4715 4732 4885 5070 5515 Size: 1255 1290 1092 753 17 153 185 445 Cuts at: 5515 325 Size: 1498 Fragments arranged by size: 1498 1290 1255 1092 753 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2831 2999 3335 Size: 274 80 33 51 1443 854 168 336 Cuts at: 3335 3799 4934 6646 96 Size: 464 1135 1712 138 Fragments arranged by size: 1712 1443 1135 854 464 336 274 168 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6648 6680 6648 Size: 32 6656 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6688 XbaI T'CTAG_A Cuts at: 4024 4024 Size: 6688 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6688 XmaI C'CCGG_G Cuts at: 1870 4013 1870 Size: 2143 4545 XmnI GAAnn'nnTTC Cuts at: 2849 3017 3437 3521 3605 3773 3857 4817 2849 Size: 168 420 84 84 168 84 960 4720 Fragments arranged by size: 4720 960 420 168 168 84 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4496 276 Size: 53 83 186 936 2962 2468 Fragments arranged by size: 2962 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI