(Circular) (Six-base) MAPSORT of: pFN21AB9751.seq Check: 4998 from: 1 to: 6523 pFN21AB9751 6523 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4333 278 Size: 53 83 186 936 2797 2468 Fragments arranged by size: 2797 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3866 1937 Size: 1929 4594 Acc65I G'GTAC_C Cuts at: 2905 3844 2905 Size: 939 5584 AclI AA'CG_TT Cuts at: 1563 4650 5023 1563 Size: 3087 373 3063 Fragments arranged by size: 3087 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2923 3424 4586 5970 6238 1632 Size: 1291 501 1162 1384 268 1917 Fragments arranged by size: 1917 1384 1291 1162 501 268 AfeI AGC'GCT Cuts at: 3281 3281 Size: 6523 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6326 AflIII A'CryG_T Cuts at: 1203 5422 6399 1203 Size: 4219 977 1327 Fragments arranged by size: 4219 1327 977 AgeI A'CCGG_T Cuts at: 5354 5354 Size: 6523 AhdI GACnn_n'nnGTC Cuts at: 5252 5252 Size: 6523 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6523 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6523 AlwNI CAG_nnn'CTG Cuts at: 4266 5838 4266 Size: 1572 4951 ApaLI G'TGCA_C Cuts at: 4580 5736 4580 Size: 1156 5367 ApoI r'AATT_y Cuts at: 1493 2057 2168 2191 2407 2682 2990 3028 3210 Size: 564 111 23 216 275 308 38 182 Cuts at: 3210 3294 3714 3832 4058 4210 6319 1493 Size: 84 420 118 226 152 2109 1697 Fragments arranged by size: 2109 1697 564 420 308 275 226 216 182 152 118 111 84 38 23 AseI AT'TA_AT Cuts at: 160 1032 5077 160 Size: 872 4045 1606 Fragments arranged by size: 4045 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6523 AvaI C'yCGr_G Cuts at: 1870 1943 3848 1870 Size: 73 1905 4545 Fragments arranged by size: 4545 1905 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2325 2358 2325 Size: 33 6490 BamHI G'GATC_C Cuts at: 3853 4220 3853 Size: 367 6156 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2225 2905 3844 5299 618 Size: 325 217 627 438 680 939 1455 1842 Fragments arranged by size: 1842 1455 939 680 627 438 325 217 BanII G_rGCy'C Cuts at: 1391 2867 3200 3842 1391 Size: 1476 333 642 4072 Fragments arranged by size: 4072 1476 642 333 BbeI G_GCGC'C Cuts at: 2229 2229 Size: 6523 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2631 2948 3261 928 Size: 971 732 317 313 4190 Fragments arranged by size: 4190 971 732 317 313 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4714 4748 1903 Size: 34 2777 34 3678 Fragments arranged by size: 3678 2777 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4415 5625 4415 Size: 1210 5313 BclI T'GATC_A Cuts at: 1548 3778 1548 Size: 2230 4293 BfrBI ATG'CAT Cuts at: 2924 2924 Size: 6523 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5134 136 Size: 107 122 71 631 605 3462 1525 Fragments arranged by size: 3462 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2946 3370 6518 2946 Size: 424 3148 2951 Fragments arranged by size: 3148 2951 424 BlpI GC'TnA_GC Cuts at: 3703 3940 3703 Size: 237 6286 Bme1580I G_kGCm'C Cuts at: 1790 4584 5740 1790 Size: 2794 1156 2573 Fragments arranged by size: 2794 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6523 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2302 2573 4254 Size: 416 53 83 399 24 885 271 1681 Cuts at: 4254 5212 442 Size: 958 1753 Fragments arranged by size: 1753 1681 958 885 416 399 271 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6523 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2756 2756 Size: 6523 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2751 3504 5183 1239 Size: 1512 753 1679 2579 Fragments arranged by size: 2579 1679 1512 753 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2430 4648 5513 5811 2430 Size: 2218 865 298 3142 Fragments arranged by size: 3142 2218 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5186 882 Size: 617 3687 2219 Fragments arranged by size: 3687 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4314 493 Size: 711 3110 2702 Fragments arranged by size: 3110 2702 711 BsaBI GATnn'nnATC Cuts at: 6517 6517 Size: 6523 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2226 4330 4712 5347 Size: 53 83 186 936 693 2104 382 635 Cuts at: 5347 275 Size: 1451 Fragments arranged by size: 2104 1451 936 693 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2867 4956 5354 5628 5775 6186 2867 Size: 2089 398 274 147 411 3204 Fragments arranged by size: 3204 2089 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2084 2114 1178 Size: 30 165 30 681 30 5587 Fragments arranged by size: 5587 681 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2063 1186 Size: 877 5646 BseYI C'CCAG_C Cuts at: 3276 5726 6222 3276 Size: 2450 496 3577 Fragments arranged by size: 3577 2450 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5950 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3973 4734 5762 1540 Size: 275 186 1972 761 1028 2301 Fragments arranged by size: 2301 1972 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2867 3200 4584 4669 5740 2867 Size: 333 1384 85 1071 3650 Fragments arranged by size: 3650 1384 1071 333 85 BsmI GAATG_Cn' Cuts at: 2224 2566 3378 4034 4127 2224 Size: 342 812 656 93 4620 Fragments arranged by size: 4620 812 656 342 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2867 3200 3842 4584 4669 5740 1391 Size: 399 1077 333 642 742 85 1071 2174 Fragments arranged by size: 2174 1077 1071 742 642 399 333 85 BspEI T'CCGG_A Cuts at: 2867 6186 2867 Size: 3319 3204 BspHI T'CATG_A Cuts at: 3589 4412 6142 3589 Size: 823 1730 3970 Fragments arranged by size: 3970 1730 823 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3878 844 Size: 3034 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4410 1394 Size: 429 2587 3507 Fragments arranged by size: 3507 2587 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5018 5192 65 Size: 1183 3770 174 1396 Fragments arranged by size: 3770 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5167 5354 1700 Size: 110 3357 187 2869 Fragments arranged by size: 3357 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6523 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6523 BssSI C'ACGA_G Cuts at: 4583 5595 4583 Size: 1012 5511 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6523 BstBI TT'CG_AA Cuts at: 3208 3712 5327 3208 Size: 504 1615 4404 Fragments arranged by size: 4404 1615 504 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6523 BstYI r'GATC_y Cuts at: 1972 2946 3370 3853 4220 4605 4622 6074 6160 Size: 974 424 483 367 385 17 1452 86 Cuts at: 6160 6172 6518 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 974 483 424 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2535 513 Size: 552 939 531 4501 Fragments arranged by size: 4501 939 552 531 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5784 Fragments arranged by size: 5784 683 56 BtsI GCAGTG_nn' Cuts at: 4039 4833 4853 4039 Size: 794 20 5709 Fragments arranged by size: 5709 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6523 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2195 2230 529 Size: 35 1631 35 4822 Fragments arranged by size: 4822 1631 35 35 DraI TTT'AAA Cuts at: 3079 3827 3079 Size: 748 5775 DraIII CAC_nnn'GTG Cuts at: 2705 2705 Size: 6523 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5530 809 Size: 507 4214 1802 Fragments arranged by size: 4214 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3970 4859 6488 Size: 54 1425 50 275 18 2140 889 1629 Cuts at: 6488 8 Size: 43 Fragments arranged by size: 2140 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3970 1537 Size: 275 2158 4090 Fragments arranged by size: 4090 2158 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2979 3501 3714 3805 4453 1353 Size: 1626 522 213 91 648 3423 Fragments arranged by size: 3423 1626 648 522 213 91 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5110 5484 5630 387 Size: 1064 138 3521 374 146 1280 Fragments arranged by size: 3521 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2865 3198 2865 Size: 333 6190 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2751 2923 3424 3504 4586 5183 5970 Size: 393 1119 172 501 80 1082 597 787 Cuts at: 5970 6238 1239 Size: 268 1524 Fragments arranged by size: 1524 1119 1082 787 597 501 393 268 172 80 EcoRI G'AATT_C Cuts at: 3210 3714 3832 4210 3210 Size: 504 118 378 5523 Fragments arranged by size: 5523 504 378 118 FspI TGC'GCA Cuts at: 5029 5029 Size: 6523 HaeII r_GCGC'y Cuts at: 1678 2229 3283 5670 1678 Size: 551 1054 2387 2531 Fragments arranged by size: 2531 2387 1054 551 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2938 2970 3091 3123 3187 Size: 32 135 32 1366 32 121 32 64 Cuts at: 3187 3219 5164 5196 5238 5270 1373 Size: 32 1945 32 42 32 2626 Fragments arranged by size: 2626 1945 1366 135 121 64 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2845 3867 4113 669 Size: 988 281 907 1022 246 3079 Fragments arranged by size: 3079 1022 988 907 281 246 HindIII A'AGCT_T Cuts at: 748 3455 748 Size: 2707 3816 HpaI GTT'AAC Cuts at: 4113 4113 Size: 6523 KasI G'GCGC_C Cuts at: 2225 2225 Size: 6523 KpnI G_GTAC'C Cuts at: 2909 3848 2909 Size: 939 5584 MfeI C'AATT_G Cuts at: 4122 4122 Size: 6523 MluI A'CGCG_T Cuts at: 6399 6399 Size: 6523 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5637 5821 1305 Size: 4332 184 2007 Fragments arranged by size: 4332 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4701 Fragments arranged by size: 4701 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3163 3594 4481 4840 4999 518 Size: 648 1997 431 887 359 159 2042 Fragments arranged by size: 2042 1997 887 648 431 359 159 MspA1I CmG'CkG Cuts at: 1606 3939 4616 5764 6009 1606 Size: 2333 677 1148 245 2120 Fragments arranged by size: 2333 2120 1148 677 245 NarI GG'CG_CC Cuts at: 2226 2226 Size: 6523 NcoI C'CATG_G Cuts at: 513 1065 2004 2535 513 Size: 552 939 531 4501 Fragments arranged by size: 4501 939 552 531 NdeI CA'TA_TG Cuts at: 387 387 Size: 6523 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6523 NotI GC'GGCC_GC Cuts at: 3970 3970 Size: 6523 NsiI A_TGCA'T Cuts at: 2926 2926 Size: 6523 NspI r_CATG'y Cuts at: 1132 1229 2769 2928 3881 5426 1132 Size: 97 1540 159 953 1545 2229 Fragments arranged by size: 2229 1545 1540 953 159 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6523 PciI A'CATG_T Cuts at: 5422 5422 Size: 6523 PflMI CCAn_nnn'nTGG Cuts at: 1108 2351 1108 Size: 1243 5280 PfoI T'CCnGG_A Cuts at: 2061 2519 2061 Size: 458 6065 PmeI GTTT'AAAC Cuts at: 3827 3827 Size: 6523 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2538 2570 4592 4624 6105 6137 2538 Size: 32 2022 32 1481 32 2924 Fragments arranged by size: 2924 2022 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3357 1613 Size: 1744 4779 PsiI TTA'TAA Cuts at: 3246 3330 3768 4093 3246 Size: 84 438 325 5676 Fragments arranged by size: 5676 438 325 84 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3111 3143 3111 Size: 32 6491 PstI C_TGCA'G Cuts at: 830 3875 830 Size: 3045 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6523 SacI G_AGCT'C Cuts at: 2867 3200 2867 Size: 333 6190 SalI G'TCGA_C Cuts at: 1936 3865 1936 Size: 1929 4594 SapI GCTCTTCn'nnn_ Cuts at: 3714 3714 Size: 6523 SbfI CC_TGCA'GG Cuts at: 3875 3875 Size: 6523 ScaI AGT'ACT Cuts at: 2657 3120 4771 6483 2657 Size: 463 1651 1712 2697 Fragments arranged by size: 2697 1712 1651 463 SfcI C'TryA_G Cuts at: 826 1045 2086 3871 5006 5687 5878 826 Size: 219 1041 1785 1135 681 191 1471 Fragments arranged by size: 1785 1471 1135 1041 681 219 191 SfoI GGC'GCC Cuts at: 2227 2227 Size: 6523 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6523 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6523 SmaI CCC'GGG Cuts at: 1872 3850 1872 Size: 1978 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2445 4627 5528 5790 820 Size: 197 926 502 2182 901 262 1553 Fragments arranged by size: 2182 1553 926 901 502 262 197 SnaBI TAC'GTA Cuts at: 493 4314 493 Size: 3821 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6523 SphI G_CATG'C Cuts at: 1132 2769 3881 1132 Size: 1637 1112 3774 Fragments arranged by size: 3774 1637 1112 SspI AAT'ATT Cuts at: 5 52 3764 4447 5 Size: 47 3712 683 2081 Fragments arranged by size: 3712 2081 683 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6523 StyI C'CwwG_G Cuts at: 513 1065 2004 2417 2535 3962 513 Size: 552 939 413 118 1427 3074 Fragments arranged by size: 3074 1427 939 552 413 118 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4550 4567 4720 4905 5350 325 Size: 1255 2970 17 153 185 445 1498 Fragments arranged by size: 2970 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2655 3118 3223 Size: 274 80 33 51 1443 678 463 105 Cuts at: 3223 3475 4769 6481 96 Size: 252 1294 1712 138 Fragments arranged by size: 1712 1443 1294 678 463 274 252 138 105 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2084 2116 6483 6515 2084 Size: 32 4367 32 2092 Fragments arranged by size: 4367 2092 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6523 XbaI T'CTAG_A Cuts at: 3859 3859 Size: 6523 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6523 XmaI C'CCGG_G Cuts at: 1870 3848 1870 Size: 1978 4545 XmnI GAAnn'nnTTC Cuts at: 2262 2592 3437 4652 2262 Size: 330 845 1215 4133 Fragments arranged by size: 4133 1215 845 330 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4331 276 Size: 53 83 186 936 2797 2468 Fragments arranged by size: 2797 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI