(Circular) (Six-base) MAPSORT of: pFN21AB9749.seq Check: 7219 from: 1 to: 6382 pFN21AB9749 6382 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2361 2361 Size: 6382 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4192 278 Size: 53 83 186 936 2656 2468 Fragments arranged by size: 2656 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3725 1937 Size: 1788 4594 Acc65I G'GTAC_C Cuts at: 3703 3703 Size: 6382 AclI AA'CG_TT Cuts at: 1563 4509 4882 1563 Size: 2946 373 3063 Fragments arranged by size: 3063 2946 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3304 4445 5829 6097 1632 Size: 1672 1141 1384 268 1917 Fragments arranged by size: 1917 1672 1384 1141 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6185 AflIII A'CryG_T Cuts at: 1203 2194 5281 6258 1203 Size: 991 3087 977 1327 Fragments arranged by size: 3087 1327 991 977 AgeI A'CCGG_T Cuts at: 5213 5213 Size: 6382 AhdI GACnn_n'nnGTC Cuts at: 2455 3232 3262 5111 2455 Size: 777 30 1849 3726 Fragments arranged by size: 3726 1849 777 30 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6382 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3563 1184 Size: 2379 4003 AlwNI CAG_nnn'CTG Cuts at: 4125 5697 4125 Size: 1572 4810 ApaI G_GGCC'C Cuts at: 2820 2820 Size: 6382 ApaLI G'TGCA_C Cuts at: 2017 2264 4439 5595 2017 Size: 247 2175 1156 2804 Fragments arranged by size: 2804 2175 1156 247 ApoI r'AATT_y Cuts at: 1493 3691 3917 4069 6178 1493 Size: 2198 226 152 2109 1697 Fragments arranged by size: 2198 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4936 160 Size: 872 3904 1606 Fragments arranged by size: 3904 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6382 AvaI C'yCGr_G Cuts at: 1870 1943 2120 2844 3045 3295 3431 3707 1870 Size: 73 177 724 201 250 136 276 4545 Fragments arranged by size: 4545 724 276 250 201 177 136 73 BamHI G'GATC_C Cuts at: 3712 4079 3712 Size: 367 6015 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2734 2942 3470 3703 5158 Size: 325 217 627 947 208 528 233 1455 Cuts at: 5158 618 Size: 1842 Fragments arranged by size: 1842 1455 947 627 528 325 233 217 208 BanII G_rGCy'C Cuts at: 1391 2211 2645 2820 3045 3375 3431 3701 1391 Size: 820 434 175 225 330 56 270 4072 Fragments arranged by size: 4072 820 434 330 270 225 175 56 BbeI G_GCGC'C Cuts at: 2738 2738 Size: 6382 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2152 2531 928 Size: 971 253 379 4779 Fragments arranged by size: 4779 971 379 253 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3260 3294 4573 4607 1903 Size: 34 1323 34 1279 34 3678 Fragments arranged by size: 3678 1323 1279 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4274 5484 4274 Size: 1210 5172 BclI T'GATC_A Cuts at: 1548 1548 Size: 6382 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3059 4993 136 Size: 107 122 71 631 605 1387 1934 1525 Fragments arranged by size: 1934 1525 1387 631 605 122 107 71 BglII A'GATC_T Cuts at: 6377 6377 Size: 6382 BlpI GC'TnA_GC Cuts at: 3799 3799 Size: 6382 Bme1580I G_kGCm'C Cuts at: 1790 2021 2268 2820 3473 4443 5599 1790 Size: 231 247 552 653 970 1156 2573 Fragments arranged by size: 2573 1156 970 653 552 247 231 BmgBI CAC'GTC Cuts at: 1318 2802 1318 Size: 1484 4898 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2460 3115 4113 Size: 416 53 83 399 24 1043 655 998 Cuts at: 4113 5071 442 Size: 958 1753 Fragments arranged by size: 1753 1043 998 958 655 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6382 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3100 3274 3100 Size: 174 6208 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2678 2922 3086 3124 3130 3142 3292 3686 Size: 1439 244 164 38 6 12 150 394 Cuts at: 3686 5042 1239 Size: 1356 2579 Fragments arranged by size: 2579 1439 1356 394 244 164 150 38 12 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3446 4507 5372 5670 3446 Size: 1061 865 298 4158 Fragments arranged by size: 4158 1061 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2777 3629 5045 882 Size: 617 1278 852 1416 2219 Fragments arranged by size: 2219 1416 1278 852 617 BsaAI yAC'GTr Cuts at: 493 1204 4173 493 Size: 711 2969 2702 Fragments arranged by size: 2969 2702 711 BsaBI GATnn'nnATC Cuts at: 6376 6376 Size: 6382 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2553 2735 4189 4571 Size: 53 83 186 936 1020 182 1454 382 Cuts at: 4571 5206 275 Size: 635 1451 Fragments arranged by size: 1454 1451 1020 936 635 382 186 182 83 53 BsaWI w'CCGG_w Cuts at: 2397 3513 4815 5213 5487 5634 6045 2397 Size: 1116 1302 398 274 147 411 2734 Fragments arranged by size: 2734 1302 1116 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2219 2249 2384 2414 3083 Size: 30 165 30 816 30 135 30 669 Cuts at: 3083 3113 3128 3158 3608 3638 1178 Size: 30 15 30 450 30 3922 Fragments arranged by size: 3922 816 669 450 165 135 30 30 30 30 30 30 30 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2240 2297 2635 2741 2950 2953 2956 2959 Size: 1054 57 338 106 209 3 3 3 Cuts at: 2959 3089 3254 3287 3343 3439 3587 1186 Size: 130 165 33 56 96 148 3981 Fragments arranged by size: 3981 1054 338 209 165 148 130 106 96 57 56 33 3 3 3 BseYI C'CCAG_C Cuts at: 2041 2701 2888 3362 5585 6081 2041 Size: 660 187 474 2223 496 2342 Fragments arranged by size: 2342 2223 660 496 474 187 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2153 1163 Size: 573 417 5392 Fragments arranged by size: 5392 573 417 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2864 3832 4593 5621 1540 Size: 275 186 863 968 761 1028 2301 Fragments arranged by size: 2301 1028 968 863 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2021 2268 2607 2645 2938 4443 4528 5599 2021 Size: 247 339 38 293 1505 85 1071 2804 Fragments arranged by size: 2804 1505 1071 339 293 247 85 38 BsmI GAATG_Cn' Cuts at: 3893 3986 3893 Size: 93 6289 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2211 2268 2607 2645 2820 2938 Size: 399 231 190 57 339 38 175 118 Cuts at: 2938 3045 3375 3431 3473 3701 4443 4528 5599 Size: 107 330 56 42 228 742 85 1071 Cuts at: 5599 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 339 330 231 228 190 175 118 107 85 57 56 42 38 BspEI T'CCGG_A Cuts at: 6045 6045 Size: 6382 BspHI T'CATG_A Cuts at: 4271 6001 4271 Size: 1730 4652 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2361 3237 3737 844 Size: 1517 876 500 3489 Fragments arranged by size: 3489 1517 876 500 BsrBI CCG'CTC Cuts at: 1394 1823 2614 4269 1394 Size: 429 791 1655 3507 Fragments arranged by size: 3507 1655 791 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2132 4877 5051 65 Size: 1183 884 2745 174 1396 Fragments arranged by size: 2745 1396 1183 884 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2417 2712 2863 2989 3281 5026 5213 Size: 110 607 295 151 126 292 1745 187 Cuts at: 5213 1700 Size: 2869 Fragments arranged by size: 2869 1745 607 295 292 187 151 126 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6382 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6382 BssSI C'ACGA_G Cuts at: 4442 5454 4442 Size: 1012 5370 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6382 BstBI TT'CG_AA Cuts at: 5186 5186 Size: 6382 BstEII G'GTnAC_C Cuts at: 1184 2593 1184 Size: 1409 4973 BstXI CCAn_nnnn'nTGG Cuts at: 2656 2656 Size: 6382 BstYI r'GATC_y Cuts at: 1972 3456 3712 4079 4464 4481 5933 6019 6031 Size: 1484 256 367 385 17 1452 86 12 Cuts at: 6031 6377 1972 Size: 346 1977 Fragments arranged by size: 1977 1484 1452 385 367 346 256 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2076 2113 2388 2539 3216 3672 Size: 552 939 72 37 275 151 677 456 Cuts at: 3672 513 Size: 3223 Fragments arranged by size: 3223 939 677 552 456 275 151 72 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2033 487 Size: 683 56 807 4836 Fragments arranged by size: 4836 807 683 56 BtsI GCAGTG_nn' Cuts at: 3898 4692 4712 3898 Size: 794 20 5568 Fragments arranged by size: 5568 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6382 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6347 DraI TTT'AAA Cuts at: 3686 3686 Size: 6382 DraIII CAC_nnn'GTG Cuts at: 2146 2593 2146 Size: 447 5935 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5389 809 Size: 507 4073 1802 Fragments arranged by size: 4073 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2861 2865 2991 Size: 54 1425 50 275 18 1031 4 126 Cuts at: 2991 3580 3675 3829 4718 6347 8 Size: 589 95 154 889 1629 43 Fragments arranged by size: 1629 1425 1031 889 589 275 154 126 95 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2861 3829 1537 Size: 275 1049 968 4090 Fragments arranged by size: 4090 1049 968 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2903 3265 3626 4312 1353 Size: 1550 362 361 686 3423 Fragments arranged by size: 3423 1550 686 362 361 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4969 5343 5489 387 Size: 1064 138 3380 374 146 1280 Fragments arranged by size: 3380 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2643 2643 Size: 6382 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2678 2922 3086 3124 3130 3142 3292 Size: 393 1046 244 164 38 6 12 150 Cuts at: 3292 3304 3686 4445 5042 5829 6097 1239 Size: 12 382 759 597 787 268 1524 Fragments arranged by size: 1524 1046 787 759 597 393 382 268 244 164 150 38 12 12 6 EcoNI CCTnn'n_nnAGG Cuts at: 3464 3464 Size: 6382 EcoO109I rG'GnC_Cy Cuts at: 2817 2925 3061 3203 3226 2817 Size: 108 136 142 23 5973 Fragments arranged by size: 5973 142 136 108 23 EcoRI G'AATT_C Cuts at: 3691 4069 3691 Size: 378 6004 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2176 2317 2389 2770 2176 Size: 141 72 381 5788 Fragments arranged by size: 5788 381 141 72 FseI GG_CCGG'CC Cuts at: 2867 2867 Size: 6382 FspI TGC'GCA Cuts at: 2063 3647 4888 2063 Size: 1584 1241 3557 Fragments arranged by size: 3557 1584 1241 HaeII r_GCGC'y Cuts at: 1678 2738 2895 3348 3530 5529 1678 Size: 1060 157 453 182 1999 2531 Fragments arranged by size: 2531 1999 1060 453 182 157 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2441 2473 3083 3115 3146 Size: 32 135 32 869 32 610 32 31 Cuts at: 3146 3178 3218 3248 3250 3280 3547 3579 3608 Size: 32 40 30 2 30 267 32 29 Cuts at: 3608 3640 5023 5055 5097 5129 1373 Size: 32 1383 32 42 32 2626 Fragments arranged by size: 2626 1383 869 610 267 135 42 40 32 32 32 32 32 32 32 32 32 31 30 30 29 2 HincII GTy'rAC Cuts at: 669 1657 1938 3726 3972 669 Size: 988 281 1788 246 3079 Fragments arranged by size: 3079 1788 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6382 HpaI GTT'AAC Cuts at: 3972 3972 Size: 6382 KasI G'GCGC_C Cuts at: 2734 2734 Size: 6382 KpnI G_GTAC'C Cuts at: 3707 3707 Size: 6382 MfeI C'AATT_G Cuts at: 3981 3981 Size: 6382 MluI A'CGCG_T Cuts at: 2194 6258 2194 Size: 4064 2318 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3105 3159 3270 5496 5680 1305 Size: 1800 54 111 2226 184 2007 Fragments arranged by size: 2226 2007 1800 184 111 54 MscI TGG'CCA Cuts at: 10 64 1489 1832 3582 3677 10 Size: 54 1425 343 1750 95 2715 Fragments arranged by size: 2715 1750 1425 343 95 54 MslI CAynn'nnrTG Cuts at: 518 1166 2261 3588 4340 4699 4858 518 Size: 648 1095 1327 752 359 159 2042 Fragments arranged by size: 2042 1327 1095 752 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2115 3798 4475 5623 5868 1606 Size: 509 1683 677 1148 245 2120 Fragments arranged by size: 2120 1683 1148 677 509 245 NaeI GCC'GGC Cuts at: 2419 2714 2865 2991 3283 2419 Size: 295 151 126 292 5518 Fragments arranged by size: 5518 295 292 151 126 NarI GG'CG_CC Cuts at: 2735 2735 Size: 6382 NcoI C'CATG_G Cuts at: 513 1065 2004 3216 513 Size: 552 939 1212 3679 Fragments arranged by size: 3679 1212 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6382 NgoMIV G'CCGG_C Cuts at: 2417 2712 2863 2989 3281 2417 Size: 295 151 126 292 5518 Fragments arranged by size: 5518 295 292 151 126 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6382 NotI GC'GGCC_GC Cuts at: 3829 3829 Size: 6382 NspI r_CATG'y Cuts at: 1132 1229 3740 5285 1132 Size: 97 2511 1545 2229 Fragments arranged by size: 2511 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6382 PciI A'CATG_T Cuts at: 5281 5281 Size: 6382 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6382 PfoI T'CCnGG_A Cuts at: 2927 2927 Size: 6382 PmeI GTTT'AAAC Cuts at: 3686 3686 Size: 6382 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4451 4483 5964 5996 4451 Size: 32 1481 32 4837 Fragments arranged by size: 4837 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2925 3203 3226 2925 Size: 278 23 6081 Fragments arranged by size: 6081 278 23 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6382 PsiI TTA'TAA Cuts at: 3952 3952 Size: 6382 PspOMI G'GGCC_C Cuts at: 2816 2816 Size: 6382 PstI C_TGCA'G Cuts at: 830 3234 3443 3552 3657 3734 830 Size: 2404 209 109 105 77 3478 Fragments arranged by size: 3478 2404 209 109 105 77 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6382 SacI G_AGCT'C Cuts at: 2645 2645 Size: 6382 SacII CC_GC'GG Cuts at: 2116 2116 Size: 6382 SalI G'TCGA_C Cuts at: 1936 3724 1936 Size: 1788 4594 SanDI GG'GwC_CC Cuts at: 2925 2925 Size: 6382 SapI GCTCTTCn'nnn_ Cuts at: 2903 2903 Size: 6382 SbfI CC_TGCA'GG Cuts at: 3734 3734 Size: 6382 ScaI AGT'ACT Cuts at: 4630 6342 4630 Size: 1712 4670 SfcI C'TryA_G Cuts at: 826 1045 3230 3439 3548 3653 3730 4865 5546 Size: 219 2185 209 109 105 77 1135 681 Cuts at: 5546 5737 826 Size: 191 1471 Fragments arranged by size: 2185 1471 1135 681 219 209 191 109 105 77 SfiI GGCCn_nnn'nGGCC Cuts at: 3059 3059 Size: 6382 SfoI GGC'GCC Cuts at: 2736 2736 Size: 6382 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6382 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6382 SmaI CCC'GGG Cuts at: 1872 2846 3047 3297 3709 1872 Size: 974 201 250 412 4545 Fragments arranged by size: 4545 974 412 250 201 SmlI C'TyrA_G Cuts at: 820 1017 1943 3461 4486 5387 5649 820 Size: 197 926 1518 1025 901 262 1553 Fragments arranged by size: 1553 1518 1025 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4173 493 Size: 3680 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6382 SphI G_CATG'C Cuts at: 1132 3740 1132 Size: 2608 3774 SspI AAT'ATT Cuts at: 5 52 4306 5 Size: 47 4254 2081 Fragments arranged by size: 4254 2081 47 StuI AGG'CCT Cuts at: 1517 3098 3485 1517 Size: 1581 387 4414 Fragments arranged by size: 4414 1581 387 StyI C'CwwG_G Cuts at: 513 1065 2004 2547 2598 2649 2682 2721 2745 Size: 552 939 543 51 51 33 39 24 Cuts at: 2745 2808 2994 3199 3216 3821 513 Size: 63 186 205 17 605 3074 Fragments arranged by size: 3074 939 605 552 543 205 186 63 51 51 39 33 24 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4409 4426 4579 4764 5209 325 Size: 1255 2829 17 153 185 445 1498 Fragments arranged by size: 2829 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4628 6340 96 Size: 274 80 33 51 1443 2651 1712 138 Fragments arranged by size: 2651 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 6342 6374 2000 Size: 32 4310 32 2008 Fragments arranged by size: 4310 2008 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6382 XbaI T'CTAG_A Cuts at: 3718 3718 Size: 6382 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3061 3061 Size: 6382 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6382 XmaI C'CCGG_G Cuts at: 1870 2844 3045 3295 3707 1870 Size: 974 201 250 412 4545 Fragments arranged by size: 4545 974 412 250 201 XmnI GAAnn'nnTTC Cuts at: 4511 4511 Size: 6382 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4190 276 Size: 53 83 186 936 2656 2468 Fragments arranged by size: 2656 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SexAI SrfI SwaI