Restriction map for
pFN21AB9747
##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AccIII AclI AcsI AflII AgeI
AhdI Alw44I AocI ApaLI ApoI
AscI AseI AsnI AspEI AviII
AvrII BamHI BbeI BbsI BcgI
BciVI BfrI BglII BlnI BpuAI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BsrDI BsrFI BsrGI
BssHII Bst1107I BstBI BstXI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco52I Eco81I EcoRI EcoRV EcoT22I
FseI FspI HgaI HincII HindII
HindIII HpaI KasI MfeI MflI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SapI
SbfI ScaI SexAI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Van91I XbaI
XhoI XhoII
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 157 GACGTC GACGT^C
Acc65I 1024 GGTACC G^GTACC
AccI 1417 GTCTAC GT^[AC][GT]AC
AcyI 157 GACGTC G[GA]^CG[CT]C
Aor51HI 57 AGCGCT AGC^GCT
Asp700I 231 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1024 GGTACC G^GTACC
BalI 543 TGGCCA TGG^CCA
BbrPI 649 CACGTG CAC^GTG
BclI 260 TGATCA T^GATCA
BfaI 130 CTAG C^TAG
BpmI 898 CTCCAG CTGGAG(16/14)
BsaAI 649 CACGTG [CT]AC^GT[GA]
BsaHI 157 GACGTC G[GA]^CG[CT]C
BsaWI 861 ACCGGA [AT]^CCGG[AT]
BseRI 31 GAGGAG GAGGAG(10/8)
BsmBI 1019 GAGACG CGTCTC(1/5)
BspHI 821 TCATGA T^CATGA
BspLU11I 1253 ACATGT A^CATGT
BstAPI 948 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1155 GGTGACC G^GT[ACGT]ACC
BstPI 1155 GGTGACC G^GT[ACGT]ACC
DrdI 1260 GACTTCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 543 TGGCCA [CT]^GGCC[GA]
Eco47III 57 AGCGCT AGC^GCT
EcoNI 463 CCTGCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1155 GGTGACC G^GT[ACGT]ACC
FbaI 260 TGATCA T^GATCA
Hin1I 157 GACGTC G[GA]^CG[CT]C
KpnI 1024 GGTACC GGTAC^C
MaeI 130 CTAG C^TAG
MluNI 543 TGGCCA TGG^CCA
MscI 543 TGGCCA TGG^CCA
MseI 596 TTAA T^TAA
PleI 985 GACTC GAGTC(4/5)
PmaCI 649 CACGTG CAC^GTG
PmlI 649 CACGTG CAC^GTG
RcaI 821 TCATGA T^CATGA
SfiI 36 GGCCTTCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 987 CTCAAG C^T[CT][GA]AG
StuI 35 AGGCCT AGG^CCT
TaqI 191 TCGA T^CGA
Tru9I 596 TTAA T^TAA
Tsp509I 717 AATT ^AATT
TthHB8I 191 TCGA T^CGA
XcmI 857 CCACACCGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 231 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 650 ACGTGT A^C[GA][CT]GT
1253 ACATGT
AlwI 1055 GATCC GGATC(4/5)
1089 GGATC
AlwNI 505 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
949 CAGGACCTG
AspHI 223 GAGCTC G[AT]GC[AT]^C
1383 GAGCTC
AspI 157 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
352 GACATTGTC
BbvCI 114 CCTCAGC CCTCAGC(-5/-2)
960 CCTCAGC
BglI 37 GCCTTCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
432 GCCAGGAAGGC
BsaBI 258 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
367 GATGACCATC
BsaI 661 GAGACC GGTCTC(1/5)
495 GGTCTC
BsgI 642 CTGCAC GTGCAG(16/14)
1291 GTGCAG
BsiHKAI 223 GAGCTC G[AT]GC[AT]^C
1383 GAGCTC
BsrBI 1376 CCGCTC CCGCTC(-3/-3)
1450 GAGCGG
BssSI 241 CACGAG CACGAG(-5/-1)
913 CACGAG
EarI 994 CTCTTC CTCTTC(1/4)
1074 CTCTTC
EcoT14I 737 CCAAGG C^C[AT][AT]GG
1494 CCAAGG
Ksp632I 994 CTCTTC CTCTTC(1/4)
1074 CTCTTC
MamI 258 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
367 GATGACCATC
NspI 1171 GCATGT [GA]CATG^[CT]
1253 ACATGT
PflFI 157 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
352 GACATTGTC
PpuMI 144 GGGACCC [GA]G^G[AT]CC[CT]
950 AGGACCT
PvuII 490 CAGCTG CAG^CTG
709 CAGCTG
SacI 223 GAGCTC GAGCT^C
1383 GAGCTC
StyI 737 CCAAGG C^C[AT][AT]GG
1494 CCAAGG
Tth111I 157 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
352 GACATTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AfaI AluI ApaI
AvaI AvaII BanI BanII BbvI
BcnI BlpI BmyI Bpu1102I BsaJI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI KspI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI TfiI
TseI Tsp45I TspRI XmaCI XmaI