(Circular) (Six-base) MAPSORT of: pFN21AB9747.seq Check: 4803 from: 1 to: 6340 pFN21AB9747 6340 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2166 4150 278 Size: 53 83 186 936 630 1984 2468 Fragments arranged by size: 2468 1984 936 630 186 83 53 AccI GT'mk_AC Cuts at: 1937 3423 3683 1937 Size: 1486 260 4594 Fragments arranged by size: 4594 1486 260 Acc65I G'GTAC_C Cuts at: 3029 3661 3029 Size: 632 5708 AclI AA'CG_TT Cuts at: 1563 4467 4840 1563 Size: 2904 373 3063 Fragments arranged by size: 3063 2904 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2537 3252 4403 5787 6055 1632 Size: 905 715 1151 1384 268 1917 Fragments arranged by size: 1917 1384 1151 905 715 268 AfeI AGC'GCT Cuts at: 2064 2064 Size: 6340 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6143 AflIII A'CryG_T Cuts at: 1203 2655 3258 5239 6216 1203 Size: 1452 603 1981 977 1327 Fragments arranged by size: 1981 1452 1327 977 603 AgeI A'CCGG_T Cuts at: 5171 5171 Size: 6340 AhdI GACnn_n'nnGTC Cuts at: 5069 5069 Size: 6340 AleI CACnn'nnGTG Cuts at: 1166 2654 3417 1166 Size: 1488 763 4089 Fragments arranged by size: 4089 1488 763 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6340 AlwNI CAG_nnn'CTG Cuts at: 2515 2959 4083 5655 2515 Size: 444 1124 1572 3200 Fragments arranged by size: 3200 1572 1124 444 ApaI G_GGCC'C Cuts at: 2053 2060 2879 3201 3465 2053 Size: 7 819 322 264 4928 Fragments arranged by size: 4928 819 322 264 7 ApaLI G'TGCA_C Cuts at: 4397 5553 4397 Size: 1156 5184 ApoI r'AATT_y Cuts at: 1493 3649 3875 4027 6136 1493 Size: 2156 226 152 2109 1697 Fragments arranged by size: 2156 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4894 160 Size: 872 3862 1606 Fragments arranged by size: 3862 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6340 AvaI C'yCGr_G Cuts at: 1870 1943 2053 2093 2146 2153 3200 3579 3665 Size: 73 110 40 53 7 1047 379 86 Cuts at: 3665 1870 Size: 4545 Fragments arranged by size: 4545 1047 379 110 86 73 53 40 7 BamHI G'GATC_C Cuts at: 3670 4037 3670 Size: 367 5973 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2255 2888 3029 3661 5116 Size: 325 217 627 468 633 141 632 1455 Cuts at: 5116 618 Size: 1842 Fragments arranged by size: 1842 1455 633 632 627 468 325 217 141 BanII G_rGCy'C Cuts at: 1391 2053 2060 2100 2232 2482 2879 3143 3201 Size: 662 7 40 132 250 397 264 58 Cuts at: 3201 3392 3465 3659 1391 Size: 191 73 194 4072 Fragments arranged by size: 4072 662 397 264 250 194 191 132 73 58 40 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5369 BbvCI CC'TCA_GC Cuts at: 2120 2966 2120 Size: 846 5494 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4531 4565 1903 Size: 34 50 34 2510 34 3678 Fragments arranged by size: 3678 2510 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4232 5442 4232 Size: 1210 5130 BclI T'GATC_A Cuts at: 1548 2265 1548 Size: 717 5623 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2048 2443 4951 Size: 107 122 71 631 605 376 395 2508 Cuts at: 4951 136 Size: 1525 Fragments arranged by size: 2508 1525 631 605 395 376 122 107 71 BglII A'GATC_T Cuts at: 6335 6335 Size: 6340 BlpI GC'TnA_GC Cuts at: 2424 3137 3248 3757 2424 Size: 713 111 509 5007 Fragments arranged by size: 5007 713 509 111 Bme1580I G_kGCm'C Cuts at: 1790 2053 2060 2258 2879 2932 3201 3465 4401 Size: 263 7 198 621 53 269 264 936 Cuts at: 4401 5557 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 936 621 269 264 263 198 53 7 BmgBI CAC'GTC Cuts at: 1318 2872 1318 Size: 1554 4786 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3433 4071 5029 Size: 416 53 83 399 24 2016 638 958 Cuts at: 5029 442 Size: 1753 Fragments arranged by size: 2016 1753 958 638 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6340 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2888 5000 1239 Size: 1649 2112 2579 Fragments arranged by size: 2579 2112 1649 Bpu10I CC'TnA_GC Cuts at: 2120 2966 2120 Size: 846 5494 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2977 4465 5330 5628 2977 Size: 1488 865 298 3689 Fragments arranged by size: 3689 1488 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2506 2660 5003 882 Size: 617 1007 154 2343 2219 Fragments arranged by size: 2343 2219 1007 617 154 BsaAI yAC'GTr Cuts at: 493 1204 2656 4131 493 Size: 711 1452 1475 2702 Fragments arranged by size: 2702 1475 1452 711 BsaBI GATnn'nnATC Cuts at: 2267 2376 6334 2267 Size: 109 3958 2273 Fragments arranged by size: 3958 2273 109 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2163 4147 4529 5164 Size: 53 83 186 936 630 1984 382 635 Cuts at: 5164 275 Size: 1451 Fragments arranged by size: 1984 1451 936 635 630 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2866 4773 5171 5445 5592 6003 2866 Size: 1907 398 274 147 411 3203 Fragments arranged by size: 3203 1907 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6115 Fragments arranged by size: 6115 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 1186 Size: 865 5475 BseYI C'CCAG_C Cuts at: 2059 2297 2712 2850 3393 3464 5543 6039 2059 Size: 238 415 138 543 71 2079 496 2360 Fragments arranged by size: 2360 2079 543 496 415 238 138 71 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2632 3317 1163 Size: 573 896 685 4186 Fragments arranged by size: 4186 896 685 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3790 4551 5579 1540 Size: 275 186 1789 761 1028 2301 Fragments arranged by size: 2301 1789 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2232 3392 4401 4486 5557 2232 Size: 1160 1009 85 1071 3015 Fragments arranged by size: 3015 1160 1071 1009 85 BsmI GAATG_Cn' Cuts at: 3851 3944 3851 Size: 93 6247 BsmBI CGTCTCn'nnnn_ Cuts at: 3018 3018 Size: 6340 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2053 2060 2100 2232 2258 2482 2879 Size: 399 263 7 40 132 26 224 397 Cuts at: 2879 2932 3143 3201 3392 3465 3659 4401 4486 Size: 53 211 58 191 73 194 742 85 Cuts at: 4486 5557 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 397 263 224 211 194 191 132 85 73 58 53 40 26 7 BspEI T'CCGG_A Cuts at: 6003 6003 Size: 6340 BspHI T'CATG_A Cuts at: 2826 4229 5959 2826 Size: 1403 1730 3207 Fragments arranged by size: 3207 1730 1403 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2781 2967 3240 3695 844 Size: 1937 186 273 455 3489 Fragments arranged by size: 3489 1937 455 273 186 BsrBI CCG'CTC Cuts at: 1394 1823 3383 3457 4227 1394 Size: 429 1560 74 770 3507 Fragments arranged by size: 3507 1560 770 429 74 BsrDI GCAATG_nn' Cuts at: 65 1248 4835 5009 65 Size: 1183 3587 174 1396 Fragments arranged by size: 3587 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4984 5171 1700 Size: 110 3174 187 2869 Fragments arranged by size: 3174 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6340 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6340 BssSI C'ACGA_G Cuts at: 2246 2918 4400 5412 2246 Size: 672 1482 1012 3174 Fragments arranged by size: 3174 1482 1012 672 BstAPI GCAn_nnn'nTGC Cuts at: 794 2959 794 Size: 2165 4175 BstBI TT'CG_AA Cuts at: 5144 5144 Size: 6340 BstEII G'GTnAC_C Cuts at: 1184 3160 1184 Size: 1976 4364 BstYI r'GATC_y Cuts at: 1972 3670 4037 4422 4439 5891 5977 5989 6335 Size: 1698 367 385 17 1452 86 12 346 Cuts at: 6335 1972 Size: 1977 Fragments arranged by size: 1977 1698 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2181 2585 3362 513 Size: 552 1116 404 777 3491 Fragments arranged by size: 3491 1116 777 552 404 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2363 487 Size: 683 56 1137 4464 Fragments arranged by size: 4464 1137 683 56 BtsI GCAGTG_nn' Cuts at: 2637 2764 3007 3160 3856 4650 4670 2637 Size: 127 243 153 696 794 20 4307 Fragments arranged by size: 4307 794 696 243 153 127 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6340 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6305 DraI TTT'AAA Cuts at: 3644 3644 Size: 6340 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3271 5347 809 Size: 507 1955 2076 1802 Fragments arranged by size: 2076 1955 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2548 3787 4676 Size: 54 1425 50 275 18 718 1239 889 Cuts at: 4676 6305 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1239 889 718 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3787 1537 Size: 275 1975 4090 Fragments arranged by size: 4090 1975 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3005 3085 4270 1353 Size: 1652 80 1185 3423 Fragments arranged by size: 3423 1652 1185 80 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4927 5301 5447 387 Size: 1064 138 3338 374 146 1280 Fragments arranged by size: 3338 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2230 3390 2230 Size: 1160 5180 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2537 2888 3252 4403 5000 5787 6055 Size: 393 905 351 364 1151 597 787 268 Cuts at: 6055 1239 Size: 1524 Fragments arranged by size: 1524 1151 905 787 597 393 364 351 268 EcoNI CCTnn'n_nnAGG Cuts at: 2472 2472 Size: 6340 EcoO109I rG'GnC_Cy Cuts at: 2020 2050 2150 2876 2956 3197 3461 2020 Size: 30 100 726 80 241 264 4899 Fragments arranged by size: 4899 726 264 241 100 80 30 EcoRI G'AATT_C Cuts at: 3649 4027 3649 Size: 378 5962 FspI TGC'GCA Cuts at: 4846 4846 Size: 6340 HaeII r_GCGC'y Cuts at: 1678 2066 2114 3471 5487 1678 Size: 388 48 1357 2016 2531 Fragments arranged by size: 2531 2016 1357 388 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4981 5013 5055 5087 1373 Size: 32 135 32 3409 32 42 32 2626 Fragments arranged by size: 3409 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3684 3930 669 Size: 988 281 1746 246 3079 Fragments arranged by size: 3079 1746 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6340 HpaI GTT'AAC Cuts at: 3930 3930 Size: 6340 KpnI G_GTAC'C Cuts at: 3033 3665 3033 Size: 632 5708 MfeI C'AATT_G Cuts at: 3939 3939 Size: 6340 MluI A'CGCG_T Cuts at: 6216 6216 Size: 6340 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5454 5638 1305 Size: 4149 184 2007 Fragments arranged by size: 4149 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2550 10 Size: 54 1425 343 718 3800 Fragments arranged by size: 3800 1425 718 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2207 2261 2321 2654 2681 3353 3356 Size: 648 1041 54 60 333 27 672 3 Cuts at: 3356 3417 3534 4298 4657 4816 518 Size: 61 117 764 359 159 2042 Fragments arranged by size: 2042 1041 764 672 648 359 333 159 117 61 60 54 27 3 MspA1I CmG'CkG Cuts at: 1606 2124 2183 2497 2587 2716 3247 3364 3756 Size: 518 59 314 90 129 531 117 392 Cuts at: 3756 4433 5581 5826 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 531 518 392 314 245 129 117 90 59 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5788 NdeI CA'TA_TG Cuts at: 387 387 Size: 6340 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6340 NotI GC'GGCC_GC Cuts at: 3787 3787 Size: 6340 NspI r_CATG'y Cuts at: 1132 1229 3180 3262 3698 5243 1132 Size: 97 1951 82 436 1545 2229 Fragments arranged by size: 2229 1951 1545 436 97 82 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6340 PciI A'CATG_T Cuts at: 3258 5239 3258 Size: 1981 4359 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6340 PmeI GTTT'AAAC Cuts at: 3644 3644 Size: 6340 PmlI CAC'GTG Cuts at: 2656 2656 Size: 6340 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4409 4441 5922 5954 4409 Size: 32 1481 32 4795 Fragments arranged by size: 4795 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2150 2956 2150 Size: 806 5534 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6340 PsiI TTA'TAA Cuts at: 3910 3910 Size: 6340 PspOMI G'GGCC_C Cuts at: 2049 2056 2875 3197 3461 2049 Size: 7 819 322 264 4928 Fragments arranged by size: 4928 819 322 264 7 PstI C_TGCA'G Cuts at: 830 2013 2793 3155 3692 830 Size: 1183 780 362 537 3478 Fragments arranged by size: 3478 1183 780 537 362 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6340 PvuII CAG'CTG Cuts at: 2497 2716 2497 Size: 219 6121 SacI G_AGCT'C Cuts at: 2232 3392 2232 Size: 1160 5180 SacII CC_GC'GG Cuts at: 2184 2588 3365 2184 Size: 404 777 5159 Fragments arranged by size: 5159 777 404 SalI G'TCGA_C Cuts at: 1936 3682 1936 Size: 1746 4594 SanDI GG'GwC_CC Cuts at: 2150 2150 Size: 6340 SbfI CC_TGCA'GG Cuts at: 3692 3692 Size: 6340 ScaI AGT'ACT Cuts at: 4588 6300 4588 Size: 1712 4628 SfcI C'TryA_G Cuts at: 826 1045 2009 2789 3151 3289 3688 4823 5504 Size: 219 964 780 362 138 399 1135 681 Cuts at: 5504 5695 826 Size: 191 1471 Fragments arranged by size: 1471 1135 964 780 681 399 362 219 191 138 SfiI GGCCn_nnn'nGGCC Cuts at: 2048 2048 Size: 6340 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6340 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6340 SmaI CCC'GGG Cuts at: 1872 2055 2148 2155 3667 1872 Size: 183 93 7 1512 4545 Fragments arranged by size: 4545 1512 183 93 7 SmlI C'TyrA_G Cuts at: 820 1017 1943 2992 4444 5345 5607 820 Size: 197 926 1049 1452 901 262 1553 Fragments arranged by size: 1553 1452 1049 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4131 493 Size: 3638 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6340 SphI G_CATG'C Cuts at: 1132 3698 1132 Size: 2566 3774 SspI AAT'ATT Cuts at: 5 52 4264 5 Size: 47 4212 2081 Fragments arranged by size: 4212 2081 47 StuI AGG'CCT Cuts at: 1517 2042 1517 Size: 525 5815 StyI C'CwwG_G Cuts at: 513 1065 2742 3499 3779 513 Size: 552 1677 757 280 3074 Fragments arranged by size: 3074 1677 757 552 280 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2666 2726 2906 2996 4367 4384 4537 Size: 1255 1086 60 180 90 1371 17 153 Cuts at: 4537 4722 5167 325 Size: 185 445 1498 Fragments arranged by size: 1498 1371 1255 1086 445 185 180 153 90 60 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3183 4586 6298 Size: 274 80 33 51 1443 1206 1403 1712 Cuts at: 6298 96 Size: 138 Fragments arranged by size: 1712 1443 1403 1206 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6300 6332 6300 Size: 32 6308 Tth111I GACn'n_nGTC Cuts at: 1316 2165 2360 1316 Size: 849 195 5296 Fragments arranged by size: 5296 849 195 XbaI T'CTAG_A Cuts at: 3676 3676 Size: 6340 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2869 2869 Size: 6340 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6340 XmaI C'CCGG_G Cuts at: 1870 2053 2146 2153 3665 1870 Size: 183 93 7 1512 4545 Fragments arranged by size: 4545 1512 183 93 7 XmnI GAAnn'nnTTC Cuts at: 2240 4469 2240 Size: 2229 4111 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2164 4148 276 Size: 53 83 186 936 630 1984 2468 Fragments arranged by size: 2468 1984 936 630 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SapI SexAI SfoI SrfI SwaI